Medical mycology journal最新文献_第2页

Genomic Analysis of Terbinafine Resistance in Microsporum canis Isolated from a Feline Dermatophytosis. 猫皮癣犬小孢子虫对特比萘芬耐药性的基因组分析。

Medical mycology journal Pub Date : 2025-01-01 DOI: 10.3314/mmj.24-00024

Honoka Nojo, Akira Watanabe, Koichi Makimura, Rui Kano

{"title":"Genomic Analysis of Terbinafine Resistance in Microsporum canis Isolated from a Feline Dermatophytosis.","authors":"Honoka Nojo, Akira Watanabe, Koichi Makimura, Rui Kano","doi":"10.3314/mmj.24-00024","DOIUrl":"10.3314/mmj.24-00024","url":null,"abstract":"Terbinafine (TBF)-resistant anthropophilic dermatophytes have recently been isolated from human patients around the world. Almost all TBF-resistant strains of dermatophytes have the amino acid substitution in the squalene epoxidase (SQLE) gene. In this study, we performed whole genome sequencing of a TBF-resistant Microsporum canis strain (designated 47C) to clarify the mechanisms underlying TBF- resistance conferred by genetic mutations. Strain 47C, isolated from a cat with feline dermatophytosis in China in 2018, was previously identified as the first TBF-resistant M. canis. Approximately 2.5 µg of genomic DNA sample was extracted from growing mycelium of 47C and sequenced using a PacBio Sequel IIe system. We mapped the 23.2 Mb genome against the reference M. canis CBS 113480 strain (assembly ASM15114v1 in GenBank) and identified 1,455 genetic variations, including substitutions, insertions and deletions (INDELs). Our analysis initially focused on whether mutations existed in the SQLE gene, which is known to encode SQLE. We discovered a T→C mutation at 1183 bp (F395L mutation) in the putative SQLE gene of strain 47C. In contrast, seven TBF-susceptible strains exhibited no mutations in their SQLE genes. The F395L mutation likely reduces the affinity of TBF for SQLE in M. canis, similar to the F397L mutation found in TBF-resistant Trichophyton rubrum and Trichophyton interdigitale.","PeriodicalId":520314,"journal":{"name":"Medical mycology journal","volume":"66 1","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A Case of Phaeohyphomycosis Caused by Exophiala xenobiotica in an Immunocompromised Host]. [1例免疫功能低下的宿主由外生菌引起的褐丝酵母菌病]。

Medical mycology journal Pub Date : 2025-01-01 DOI: 10.3314/mmj.24-00013

Reiko Saito, Tomomichi Shimizu, Akio Kondoh, Takashi Matsuyama, Akitoshi Hisada, Takashi Yaguchi, Tomotaka Sato, Tomotaka Mabuchi

引用次数: 0

Report on the 8th Congress of the Asia-Pacific Society for Medical Mycology (APSMM 2024). 第8届亚太医学真菌学学会（APSMM 2024）大会报告。

Medical mycology journal Pub Date : 2025-01-01 DOI: 10.3314/mmj.25.003

Takashi Sugita, Ryoji Tsuboi, Kazutoshi Harada, Koichi Izumikawa, Yoshitsugu Miyazaki, Kazutoshi Shibuya, Ruoyu Li

引用次数: 0

CRISPR-Cas9 RNP-Mediated Deletion of ERG25 in Non-albicans Candida Species, Including Candida auris. CRISPR-Cas9 rnp介导的非白色念珠菌（包括耳念珠菌）ERG25缺失

Medical mycology journal Pub Date : 2025-01-01 DOI: 10.3314/mmj.24-00023

Michiyo Okamoto, Kaname Sasamoto, Azusa Takahashi-Nakaguchi, Fujiang Zhao, Masashi Yamaguchi, Hiroji Chibana

{"title":"CRISPR-Cas9 RNP-Mediated Deletion of ERG25 in Non-albicans Candida Species, Including Candida auris.","authors":"Michiyo Okamoto, Kaname Sasamoto, Azusa Takahashi-Nakaguchi, Fujiang Zhao, Masashi Yamaguchi, Hiroji Chibana","doi":"10.3314/mmj.24-00023","DOIUrl":"https://doi.org/10.3314/mmj.24-00023","url":null,"abstract":"The incidence of infections caused by non-albicans Candida (NAC) species, including Candida glabrata and Candida tropicalis, has recently increased. Furthermore, Candida auris, a multidrug-resistant yeast, poses a serious threat to global health. The development of novel antifungal agents with alternative mechanisms of action is necessary to combat these fungi. However, genetic analyses of the virulence factors in these NAC species are insufficient for this purpose. Recent advancements in the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system have facilitated enhanced the genetic analysis of NAC species. The RNP-based system, in which the Cas9-gRNA complex is assembled in vitro and introduced into cells, offers a simplified approach to genetic modification, eliminating the need for species-specific plasmids. Previous our research identified the ERG25 gene, which encodes C-4 sterol methyl oxidase, as a promising antifungal target in C. glabrata. This study demonstrated deletion of the ERG25 homolog in C. glabrata and C. auris using an RNP-based CRISPR-Cas9 system. The deletion of ERG25 in C. auris and C. glabrata indicated that Erg25 is crucial for the survival of these pathogenic yeasts within the host. Furthermore, we have successfully deleted the ERG25 alleles in C. tropicalis and Candida parapsilosis, demonstrating the effectiveness of using both the CRISPR-Cas9 and Cre-loxP systems in these species for the first time.","PeriodicalId":520314,"journal":{"name":"Medical mycology journal","volume":"66 2","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of Collaboration Between Microbiology Laboratories, Pathology Departments, Dermatologists, and Clinical Mycologists in the Diagnosis of Fungal Infectious Diseases Through the Accurate Detection of Fungi. 微生物实验室、病理部门、皮肤科医生和临床真菌学家通过真菌的准确检测在真菌感染性疾病诊断中的重要性。

Medical mycology journal Pub Date : 2025-01-01 DOI: 10.3314/mmj.25.002

Shigeki Misawa

{"title":"Importance of Collaboration Between Microbiology Laboratories, Pathology Departments, Dermatologists, and Clinical Mycologists in the Diagnosis of Fungal Infectious Diseases Through the Accurate Detection of Fungi.","authors":"Shigeki Misawa","doi":"10.3314/mmj.25.002","DOIUrl":"10.3314/mmj.25.002","url":null,"abstract":"Fungal detection by microbiology testing is essential for diagnosing fungal infections. In routine clinical microbiology, limited specimens are automatically covered for fungi; hence, detection of fungi from many other clinical specimens depends on requests from clinicians. Microbiology laboratories should collaborate with clinicians to build a system to ensure that tests can be requested. Microbiology laboratories determine culture conditions based on the epidemiology of fungal infections by specimen types. If a fungus is detected on microscopic examination, it is highly significant as a causative, and if detected on pathological examination, it can be additionally differentiated from other fungal species. Microbiology laboratories, clinicians, and pathology departments must share information and improve fungal detection. When a small number of fungi are detected in culture, clinical backgrounds are essential for interpretation, and collaboration with a clinician and assistance from a dermatologist or clinical mycologist can make the diagnosis more certain.Collaboration with microbiology laboratories, pathology departments, dermatologists, and clinical mycologists can help clinicians and lead to diagnostic support for fungal infections.","PeriodicalId":520314,"journal":{"name":"Medical mycology journal","volume":"66 2","pages":"75-85"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Case of Invasive Rhinosinusitis Caused by Penicillium brasilianum. 巴西青霉引起侵袭性鼻窦炎1例。

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.24-00015

Yuki Hirao, Hiroshi Morioka, Shinya Ozaki, Makoto Kawachi, Sayaka Ban, Takashi Yaguchi, Akira Watanabe

引用次数: 0

A Case of Tinea Barbae due to Trichophyton mentagrophytes Presenting as a Tumor. 由毛癣菌引起的斑癣以肿瘤表现1例。

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.24-00018

Hiroshi Tanabe

{"title":"A Case of Tinea Barbae due to Trichophyton mentagrophytes Presenting as a Tumor.","authors":"Hiroshi Tanabe","doi":"10.3314/mmj.24-00018","DOIUrl":"10.3314/mmj.24-00018","url":null,"abstract":"I report a case of tinea barbae presenting as a tumor in the philtrum of a man in his thirties with comorbid alcoholic liver disease. The patient also had tinea on the auricles, neck, and feet, with direct microscopy confirming the presence of dermatophytes at all sites. A history of multiple pet ownership was noted. The causative organism in the philtrum was identified as the zoophilic fungus Trichophyton mentagrophytes, while the tinea on the feet was caused by the anthropophilic fungus Trichophyton interdigitale ITS genotype II. Despite abnormal liver enzymes, the patient was cured after a three-month course of oral terbinafine following cessation of alcohol intake. This case, encountered by the author approximately 20 years ago, was initially identified as Arthroderma vanbreuseghemii using PCR-RFLP analysis of the ITS region of rRNA genes. Recent sequencing analysis of preserved strains reidentified the organism as T. mentagrophytes. The alignment of this strain showed a query cover of 100% and a percent identity (Per.ident) of 99.84%, matching with T. mentagrophytes ITS genotype VII. As a dermatologist, it is crucial to continuously monitor the evolving taxonomy of fungi and its clinical implications.","PeriodicalId":520314,"journal":{"name":"Medical mycology journal","volume":"65 4","pages":"93-98"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dermoscopic Examination of Tinea Nigra on the Plantar Foot Unveils a Parallel Ridge Pattern Mimicking Acral-Lentiginous Melanoma. 足底黑癣的皮肤镜检查显示平行脊型，酷似肢端色斑黑色素瘤。

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.24-00010

Mako Saikawa, Utako Kimura, Yuka Ikari, Ayaka Miyata, Takahide Kaneko, Masataro Hiruma, Takashi Yaguchi, Masaru Tanaka, Kenji Takamori, Yasushi Suga

{"title":"Dermoscopic Examination of Tinea Nigra on the Plantar Foot Unveils a Parallel Ridge Pattern Mimicking Acral-Lentiginous Melanoma.","authors":"Mako Saikawa, Utako Kimura, Yuka Ikari, Ayaka Miyata, Takahide Kaneko, Masataro Hiruma, Takashi Yaguchi, Masaru Tanaka, Kenji Takamori, Yasushi Suga","doi":"10.3314/mmj.24-00010","DOIUrl":"https://doi.org/10.3314/mmj.24-00010","url":null,"abstract":"A man in his thirties patient visited his previous physician with the chief complaint of a pigmented macule on the sole of his left foot that had steadily grown over the past two years. A dermoscopic examination of the lesion showed a parallel ridge pattern (PRP); therefore, acral-lentiginous melanoma (ALM) was suspected, and the patient was referred to our hospital for a more detailed examination. On closer inspection, PRP had a well-defined and irregular border with no variation in color, which is atypical for ALM which is characterized by asymmetrical structure and variation in colors. Therefore, a skin biopsy was performed for a definite diagnosis, and the histopathological findings showed mycelia in the horny layers. Fungal culture revealed Hortaea werneckii, and the patient was diagnosed with tinea nigra (TN). Although TN is a rare fungal infection in Japan, we emphasize that this fungal infection should be noted as a mimic of ALM and should be considered when PRP is detected on the palms and soles by dermoscopic examination.","PeriodicalId":520314,"journal":{"name":"Medical mycology journal","volume":"65 4","pages":"103-106"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overview of Fungi Classified as Risk Group 3 by the Japanese Society for Medical Mycology. 日本医学真菌学学会分类为风险组3的真菌概述。

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.24.008

Masato Tashiro, Takahiro Takazono, Koichi Izumikawa

{"title":"Overview of Fungi Classified as Risk Group 3 by the Japanese Society for Medical Mycology.","authors":"Masato Tashiro, Takahiro Takazono, Koichi Izumikawa","doi":"10.3314/mmj.24.008","DOIUrl":"10.3314/mmj.24.008","url":null,"abstract":"Fungi are categorized into Risk or Hazard Groups, depending on their potential to harm humans, with groupings ranging from 1 (least risky) to 4 (most risky). Similarly, Biosafety or Containment Levels ranging from 1 to 4 denote the level of control over microorganisms in laboratories. This review focuses on fungi categorized as Risk Group 3 by the Biosafety Committee of the Japanese Society for Medical Mycology. One such fungus is Blastomyces dermatitidis, which is primarily found in North America and causes blastomycosis, a condition that can result in a pulmonary or disseminated disease. It is classified as Risk Group 3, as substantiated by 11 laboratory-acquired infections and two fatalities. Cladophialophora bantiana, commonly found in Asia, causes brain abscesses and is also classified as Risk Group 3 due to a high mortality rate of 65.2%. Coccidioides immitis/posadasii, found in the Americas, can cause severe respiratory conditions with the potential for dissemination. Their Risk Group 3 classification is substantiated by a history of laboratory-acquired infections. Histoplasma capsulatum, which is found globally, causes a range of infections, from asymptomatic to fatal. Its classification as Risk Group 3 is substantiated by 81 laboratory-acquired infections and one fatality. Rhinocladiella mackenziei, which primarily affects the nervous system, has a mortality rate of 92.5% and is also classified as Risk Group 3. These fungi necessitate strict containment measures in laboratories owing to their high mortality rates or reported cases of laboratory-acquired infections and fatalities, emphasizing the critical need for heightened awareness and stringent safety protocols.","PeriodicalId":520314,"journal":{"name":"Medical mycology journal","volume":"65 4","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Silver Staining in Artificial Cytology Samples of Filamentous Fungi. 丝状真菌人工细胞学样品银染色方法的优化。

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.24-00009

Kazuki Amemiya, Sota Sadamoto, Ken Miyazawa, Minoru Shinozaki, Sayaka Ban, Yoshitsugu Miyazaki, Somay Yamagata Murayama, Kazutoshi Shibuya, Naobumi Tochigi

引用次数: 0