npj antimicrobials and resistance最新文献

Metabolic state-driven nutrient-based approach to combat bacterial antibiotic resistance. 代谢状态驱动的基于营养的方法对抗细菌抗生素耐药性。

npj antimicrobials and resistance Pub Date : 2025-04-04 DOI: 10.1038/s44259-025-00092-5

Bo Peng, Hui Li, Xuan-Xian Peng

引用次数: 0

Evaluating the protective effects of Aurodox in a murine model of Shiga toxin-producing Escherichia coli. 评价氧化还原对产志贺毒素大肠杆菌小鼠模型的保护作用。

npj antimicrobials and resistance Pub Date : 2025-04-01 DOI: 10.1038/s44259-025-00094-3

Rebecca E McHugh, Liam M Rooney, David R Mark, Kabo R Wale, Megan Clapperton, Gail McConnell, Paul A Hoskisson, Gillian R Douce, Andrew J Roe

{"title":"Evaluating the protective effects of Aurodox in a murine model of Shiga toxin-producing Escherichia coli.","authors":"Rebecca E McHugh, Liam M Rooney, David R Mark, Kabo R Wale, Megan Clapperton, Gail McConnell, Paul A Hoskisson, Gillian R Douce, Andrew J Roe","doi":"10.1038/s44259-025-00094-3","DOIUrl":"10.1038/s44259-025-00094-3","url":null,"abstract":"Shiga Toxin-Producing E. coli (STEC) are a group of acute small intestine pathogens responsible for foodborne outbreaks of bloody diarrhoea. The expression of Shiga toxins (Stx) carried by STEC can initiate Haemolytic Uremic Syndrome (HUS), a major cause of acute renal failure in children. Here, we investigate the anti-virulence potential of Aurodox - a natural product of Streptomyces goldiniensis. Previously, we have shown that Aurodox downregulates the expression of the T3SS, inhibiting epithelial cell colonisation in vitro. Here, we use the Citrobacter rodentium DBS770 (Cr Stx2dact) model of STEC infection to demonstrate that Aurodox protects mice against Citrobacter rodentium-associated colonic hyperplasia and Stx-mediated renal injury. Given antibiotic-associated dysbiosis of the gut is associated with inflammation and the emergence of opportunistic pathogens, we examined the effect of Aurodox on the faecal bacteriome. We show that although the microbial community is altered following Aurodox treatment, changes are distinct from those associated with traditional antibiotic therapies.","PeriodicalId":520007,"journal":{"name":"npj antimicrobials and resistance","volume":"3 1","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repurposing antimicrobials with ultrasound-triggered nanoscale systems for targeted biofilm drug delivery. 用超声波触发的纳米级系统重新利用抗菌剂进行靶向生物膜药物输送。

npj antimicrobials and resistance Pub Date : 2025-04-01 DOI: 10.1038/s44259-025-00086-3

Victor Choi, Dario Carugo, Eleanor Stride

{"title":"Repurposing antimicrobials with ultrasound-triggered nanoscale systems for targeted biofilm drug delivery.","authors":"Victor Choi, Dario Carugo, Eleanor Stride","doi":"10.1038/s44259-025-00086-3","DOIUrl":"10.1038/s44259-025-00086-3","url":null,"abstract":"Chronic infections represent a major clinical challenge due to the enhanced antimicrobial tolerance of biofilm-dwelling bacteria. To address this challenge, an ultrasound-responsive nanoscale drug delivery platform (nanodroplets) is presented in this work, loaded with four different antimicrobial agents, capable of simultaneous biofilm disruption and targeted antimicrobial delivery. When loaded, a robust protective effect against clinically-derived MRSA and ESBL Gram-positive and Gram-negative planktonic isolates was shown in vitro. Upon application of therapeutic ultrasound, an average 7.6-fold, 44.4-fold, and 25.5-fold reduction was observed in the antibiotic concentrations compared to free drug required to reach the MBC, MBEC and complete persister eradication levels, respectively. Nanodroplets substantially altered subcellular distribution of encapsulated antimicrobials, enhancing accumulation of antimicrobials by 11.1-fold within the biofilm-residing bacteria's cytoplasm compared to treatment with unencapsulated drugs. These findings illustrate the potential of this multifunctional platform to overcome the critical penetration and localization limitations of antimicrobials within biofilms, opening potential new avenues in the treatment of chronic clinical infections.","PeriodicalId":520007,"journal":{"name":"npj antimicrobials and resistance","volume":"3 1","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting social traits for clinical applications in bacteria and viruses. 利用细菌和病毒的社会特征进行临床应用。

npj antimicrobials and resistance Pub Date : 2025-03-28 DOI: 10.1038/s44259-025-00091-6

Ashleigh S Griffin, Asher Leeks

引用次数: 0

Integrating socioeconomic deprivation indices and electronic health record data to predict antimicrobial resistance. 整合社会经济贫困指数和电子健康记录数据，预测抗菌药耐药性。

npj antimicrobials and resistance Pub Date : 2025-03-28 DOI: 10.1038/s44259-025-00090-7

Marlon I Diaz, Lauren N Cooper, John J Hanna, Alaina M Beauchamp, Tanvi A Ingle, Abdi D Wakene, Zachary Most, Trish Perl, Chaitanya Katterpalli, Tony Keller, Clark Walker, Christoph U Lehmann, Richard J Medford

{"title":"Integrating socioeconomic deprivation indices and electronic health record data to predict antimicrobial resistance.","authors":"Marlon I Diaz, Lauren N Cooper, John J Hanna, Alaina M Beauchamp, Tanvi A Ingle, Abdi D Wakene, Zachary Most, Trish Perl, Chaitanya Katterpalli, Tony Keller, Clark Walker, Christoph U Lehmann, Richard J Medford","doi":"10.1038/s44259-025-00090-7","DOIUrl":"https://doi.org/10.1038/s44259-025-00090-7","url":null,"abstract":"We developed machine learning models to predict the presence of AMR organisms in blood cultures obtained at the first patient encounter, offering a new and inspiring direction for antimicrobial resistance management. Three supervised machine learning classifiers were used: penalized logistic regression, random forest, and XGBoost, which were used to classify five AMR organisms: ESBL, CRE, AmpC, MRSA, and VRE. The random forest and XGBoost models performed best, with AUC-ROC values of 0.70 and 92.9% negative predictive value, respectively. The multi-class random forest model's AUC-ROC values ranged from 0.80-0.95. Our models highlight how the combination of ADI and SVI increased the predictive power. This approach could reduce costs and mitigate the global public health threat posed by antibiotic-resistant infections. Machine learning techniques can predict antimicrobial-resistant infections in suspected cultures using patient data from EHRs, enabling clinicians to make targeted prescribing decisions and mitigate resistance development.","PeriodicalId":520007,"journal":{"name":"npj antimicrobials and resistance","volume":"3 1","pages":"21"},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How AI can help us beat AMR. 人工智能如何帮助我们战胜抗生素耐药性。

npj antimicrobials and resistance Pub Date : 2025-03-13 DOI: 10.1038/s44259-025-00085-4

Autumn Arnold, Stewart McLellan, Jonathan M Stokes

引用次数: 0

Innovative perspectives on the discovery of small molecule antibiotics. 小分子抗生素发现的创新视角。

npj antimicrobials and resistance Pub Date : 2025-03-13 DOI: 10.1038/s44259-025-00089-0

Silvia T Cardona, A S M Zisanur Rahman, Julieta Novomisky Nechcoff

引用次数: 0

R-pyocins as targeted antimicrobials against Pseudomonas aeruginosa. R-pyocins作为铜绿假单胞菌的靶向抗菌剂。

npj antimicrobials and resistance Pub Date : 2025-02-28 DOI: 10.1038/s44259-025-00088-1

Madeline Mei, Isaac Estrada, Stephen P Diggle, Joanna B Goldberg

引用次数: 0

Aspergillus fumigatus mitogenomes and their influence on azole-resistant and -susceptible populations. 曲霉有丝分裂基因组及其对唑类抗性和易感人群的影响。

npj antimicrobials and resistance Pub Date : 2025-02-27 DOI: 10.1038/s44259-025-00083-6

Endrews Delbaje, Laís Pontes, Johanna Rhodes, Jacob Steenwyk, Ling Lu, Thaila F Dos Reis, Antonis Rokas, Gustavo H Goldman

{"title":"Aspergillus fumigatus mitogenomes and their influence on azole-resistant and -susceptible populations.","authors":"Endrews Delbaje, Laís Pontes, Johanna Rhodes, Jacob Steenwyk, Ling Lu, Thaila F Dos Reis, Antonis Rokas, Gustavo H Goldman","doi":"10.1038/s44259-025-00083-6","DOIUrl":"10.1038/s44259-025-00083-6","url":null,"abstract":"The role of the fungal mitochondria goes far beyond energy metabolism. The genomes of 318 Aspergillus fumigatus clinical and environmental isolates from different geographic origins were analyzed aiming to study the mitochondrial sequences from populations sensitive and resistant to azoles. Our results show that A. fumigatus mitogenomic sequences are very conserved and only show variation in small intergenic regions and one intronic sequence in the cox3 gene. Furthermore, a genome-wide association analysis of accessory mitochondrial genes revealed potential mitochondria-based genotypes that may interact synergistically with the ergosterol biosynthesis pathway to confer the resistant phenotype. This includes a mutation in the AMID-like mitochondrial oxidoreductase (aifA, AFUA_3G01290) and the absence of the mitochondrial carrier protein (pet8, AFUA_8G01400). Deletion of these genes did not change the azole-susceptibility but increased the azole-persistence, suggesting mitochondrial genes could be involved in azole-persistence. Our work opens new hypotheses for the involvement of mitochondria in A. fumigatus azole-resistance.","PeriodicalId":520007,"journal":{"name":"npj antimicrobials and resistance","volume":"3 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advantages and limitations of large language models for antibiotic prescribing and antimicrobial stewardship. 用于抗生素处方和抗菌药物管理的大型语言模型的优点和局限性。

npj antimicrobials and resistance Pub Date : 2025-02-27 DOI: 10.1038/s44259-025-00084-5

Daniele Roberto Giacobbe, Cristina Marelli, Bianca La Manna, Donatella Padua, Alberto Malva, Sabrina Guastavino, Alessio Signori, Sara Mora, Nicola Rosso, Cristina Campi, Michele Piana, Ylenia Murgia, Mauro Giacomini, Matteo Bassetti

引用次数: 0