Biophotonics discovery最新文献

{"title":"Exploring racial differences in second-harmonic-generation-based prognostic indicators of metastasis in breast and colon cancer.","authors":"Tresa M Elias, Danielle E Desa, Edward B Brown, Showmick Paul, Gabriel A Ramirez, Bradley M Turner, Kelley Madden, Raul S Gonzalez, Anna Weiss, Edward B Brown","doi":"10.1117/1.bios.2.2.022703","DOIUrl":"10.1117/1.bios.2.2.022703","url":null,"abstract":"<p><strong>Significance: </strong>Second-harmonic generation (SHG) analysis of collagen internal structure and overall organization in the tumor microenvironment may enhance current metastasis prediction methods, which do not prognosticate with the same accuracy for patients of different races. For these optical tools to be clinically available, a multicenter trial is needed. We investigate if SHG-based prognostic signals vary with patient race, providing insight for designing such a trial.</p><p><strong>Aim: </strong>SHG imaging was performed on colon adenocarcinoma (CRC) and invasive ductal carcinoma (IDC) patient samples to derive two prognostic indicators. We assessed the association between these indicators and patient race.</p><p><strong>Approach: </strong>SHG images were analyzed as previously described to determine the forward- to backward-SHG scattering ratio (F/B) and fiber angle variability (FAV). Both prognostic measurements were compared between Black and White patients.</p><p><strong>Results: </strong>In the IDC cohort, F/B from the tumor-stroma interface differed significantly between demographic groups. For the CRC cohort, a trend was observed in the tumor-stroma interface and tumor bulk. FAV did not vary by race in either cohort.</p><p><strong>Conclusions: </strong>F/B variation with patient race suggests the relationship between F/B and metastatic outcome may vary with patient race. These findings highlight the potential need for race-specific prognostic algorithms to improve metastasis prediction for all patients.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144985247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free optical microscopy with artificial intelligence: a new paradigm in pathology.","authors":"Chiho Yoon, Eunwoo Park, Donggyu Kim, Byullee Park, Chulhong Kim","doi":"10.1117/1.BIOS.2.2.020901","DOIUrl":"10.1117/1.BIOS.2.2.020901","url":null,"abstract":"<p><strong>Significance: </strong>Pathological examination is essential for diagnosing diseases in tissues such as cancer but involves labor-intensive and time-consuming processes. Label-free optical microscopy has emerged as a promising alternative that offers the ability to visualize tissue structures without the need for histochemical staining. Further, the integration of artificial intelligence (AI) into label-free microscopy has the potential to streamline the overall pathological diagnostic process.</p><p><strong>Aim: </strong>We aim to review the use of AI-assisted label-free optical microscopy in revolutionizing pathological workflows.</p><p><strong>Approach: </strong>We examine the integration of AI with label-free optical microscopy techniques and assess its overall impact on the pathological workflow. We evaluate how AI enhances each stage of label-free pathology, including specimen preparation, label-free imaging, virtual staining, and diagnostic analysis.</p><p><strong>Results: </strong>Label-free optical microscopy with AI has significantly improved the entire pathological workflow. AI assists specimen preparation with high efficiency, enhances label-free imaging with high resolution and speed, and enables cost-effective virtual staining with high throughput and automatic diagnostic analysis with high accuracy.</p><p><strong>Conclusions: </strong>AI-aided label-free optical microscopy enhances diagnostic speed, accuracy, and specimen preservation, offering a transformative approach that could redefine traditional pathology workflows and improve clinical outcomes.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"2 2","pages":"020901"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell Raman spectroscopy detects pediatric focal cortical dysplasia.","authors":"Trang Tran, Frederick Dallaire, Joshua Sonnen, Romain Cayrol, Frédéric Leblond, Roy W R Dudley","doi":"10.1117/1.BIOS.2.1.015002","DOIUrl":"10.1117/1.BIOS.2.1.015002","url":null,"abstract":"<p><strong>Significance: </strong>Focal cortical dysplasia (FCD) type II is the leading cause of drug-resistant focal epilepsy in children. While surgical resection offers the only definitive cure, its success is hindered by the challenge of precisely identifying the lesion and its boundaries. Despite advancements in neuroimaging, FCD type II often remains elusive, complicating surgical planning and outcome optimization. Enhanced detection methods are crucial to improving the precision of resection and, ultimately, achieving seizure freedom in affected patients.</p><p><strong>Aim: </strong>Advanced techniques for detecting FCD type II margins during surgery are critically needed to enhance postoperative outcomes. Spontaneous Raman spectroscopy is a label-free optical method that allows the characterization of the tissue's biochemical composition. The goal of this proof-of-concept study was to compare-in pediatric patients-the spectral signature of abnormal cells in FCD tissue with cells associated with the normal cortex.</p><p><strong>Approach: </strong>A Raman microspectroscopy imaging workflow was developed and applied to 70 surgical specimens from 30 focal epilepsy patients diagnosed with FCD type II. Raman spectra from individual cells were recorded from FCD type II specimens (dysmorphic neurons and balloon cells) and normal brains (neurons). Machine learning models (support vector machines) were trained, validated, and tested to distinguish FCD tissue from the normal brain as well as to distinguish between two disease subtypes, i.e., FCD types IIa and IIb.</p><p><strong>Results: </strong>A total of 1420 single-cell spectra were acquired and spectral differences determined between FCD type II and normal cortex, as well as between FCD type IIa and type IIb. Machine learning distinguished FCD type II from the normal cortex with 96% accuracy, 100% sensitivity, and 95% specificity. FCD types IIa and IIb specimens were distinguished with 92% accuracy, 100% sensitivity, and 86% specificity.</p><p><strong>Conclusions: </strong>The Raman spectroscopy signature of single cells associated with FCD tissue was established. This provides credence to the hypothesis that Raman spectroscopy as a technique-if implemented using a fiber optics system-has the potential for safely optimizing the extent of FCD type II resection in pediatric focal epilepsy surgery. In addition, this technique provides insights into multiple biochemical alterations within dysplastic tissues, which may contribute to the underlying mechanisms of epileptogenesis.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"2 1","pages":"015002"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved performance and design of a low-cost laparoscope to enable laparoscopic surgery in low-income countries.","authors":"Anne Christine Barnes, Michele L Kaluzienski, Jason Chen, Tri T Quang, Talya Simcox, Paula Kworekwa, Rebecca Kaaya, Robert Ssekitoleko, Kevin Aroom, John Rzasa, Tamara Fitzgerald, Jenna L Mueller","doi":"10.1117/1.BIOS.2.2.022302","DOIUrl":"10.1117/1.BIOS.2.2.022302","url":null,"abstract":"<p><strong>Significance: </strong>Laparoscopy has become the standard of care for surgery in the chest and abdomen but is typically unavailable in low- and middle-income countries (LMICs). This inaccessibility is partly due to a high initial equipment purchase price, ongoing maintenance costs, unreliable electricity, shortage of biomedical technicians, and limited sterilization facilities.</p><p><strong>Aim: </strong>To address these challenges, a low-cost, durable, reusable laparoscopic system (KeyScope) was designed for use in LMICs.</p><p><strong>Approach: </strong>Through an iterative human-centered design approach, the performance of the KeyScope was optimized by comparing standard image quality metrics to a commercially available standard-of-care (SOC) laparoscope.</p><p><strong>Results: </strong>The latest version of the KeyScope has comparable or better resolving power, lens distortion, field of view, depth of field, and color reproduction accuracy to a SOC laparoscope (Precision Ideal Eyes HD Laparoscope, Stryker) at working distances commonly used during laparoscopic surgery (3 to 13 cm). Interference from electrocautery was eliminated by shielding the camera ground from the housing ground. Finally, the entire KeyScope is equipped for production and implementation in sub-Saharan Africa, as the device can be submerged in Cidex and can be easily assembled in Uganda in under 1 h.</p><p><strong>Conclusion: </strong>These results suggest that the KeyScope has achieved the performance criteria needed for surgical care in LMICs.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"2 2","pages":"022302"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid diffuse optical appraisal of peripheral and cerebral changes in critically ill patients receiving red blood cell transfusion.","authors":"Susanna Tagliabue, Anna Rey-Perez, Lourdes Exposito, Andrés F Jimenez, Sara Valles Angulo, Federica Maruccia, Jonas B Fischer, Michal Kacprzak, Maria A Poca, Turgut Durduran","doi":"10.1117/1.BIOS.2.1.015001","DOIUrl":"10.1117/1.BIOS.2.1.015001","url":null,"abstract":"<p><p><b>Significance</b>: Red blood cell transfusions (RBCTs) to restore hemoglobin concentration and hematocrit values in anemic patients are debated in critical illness. <b>Aim</b>: We used non-invasive hybrid diffuse optics (DO) to study RBCT's effect on the brain and peripheral muscle evaluated in critically ill patients. <b>Approach</b>: Critically ill patients admitted to the neurotrauma intensive care unit of the Vall d'Hebron University Hospital that required blood transfusion were included in the study and continuously monitored by DO methods alongside time-specific blood gas samples. <b>Results</b>: In 14 patients, blood-derived hemoglobin, hematocrit, and DO-derived metabolic rate of oxygen, oxy- and total hemoglobin significantly increased after RBCT. Oxygen extraction fraction significantly decreased. Blood flow significantly increased only in muscles. Deoxy-hemoglobin concentration remained unchanged. No significant differences were found between cerebral and peripheral signals post-RBCT, except for the metabolic rate of oxygen (higher in muscle). <b>Conclusion</b>: Hybrid DO monitoring quantifies cerebral and peripheral microvascular changes during red blood cell transfusion, enabling insights into oxygen metabolism and advancing critical care management.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"2 1","pages":"015001"},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical imaging provides flow-cytometry-like single-cell level analysis of HIF-1<i>α</i>-mediated metabolic changes in radioresistant head and neck squamous carcinoma cells.","authors":"Jing Yan, Carlos Frederico Lima Goncalves, Pranto Soumik Saha, Cristina M Furdui, Caigang Zhu","doi":"10.1117/1.bios.2.1.012702","DOIUrl":"10.1117/1.bios.2.1.012702","url":null,"abstract":"<p><strong>Significance: </strong>Radioresistance remains a significant problem for head and neck squamous cell carcinoma (HNSCC) patients. To mitigate this, the cellular and molecular pathways used by radioresistant HNSCC that drive recurrence must be studied.</p><p><strong>Aim: </strong>We aim to demonstrate optical imaging strategies to provide flow cytometry-like single-cell level analysis of hypoxia-inducible factor 1-alpha (HIF-1α)-mediated metabolic changes in the radioresistant and radiosensitive HNSCC cells but in a more efficient, cost-effective, and non-destructive manner. Through both optical imaging and flow cytometry studies, we will reveal the role of radiation-induced HIF-1α overexpression and the following metabolic changes in the radioresistance development for HNSCC.</p><p><strong>Approach: </strong>We optimized the use of two metabolic probes: 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG) (to report glucose uptake) and Tetramethylrhodamine ethyl ester (TMRE) (to report mitochondrial membrane potential) with both a standard fluorescence microscope and a flow cytometry device, to report the changes in metabolism between radioresistant (rSCC-61) and radiosensitive (SCC-61) HNSCC cell lines under radiation stresses with or without HIF-1<i>α</i> inhibition.</p><p><strong>Results: </strong>We found that the matched HNSCC cell lines had different baseline metabolic phenotypes, and their metabolism responded differently to radiation stress along with significantly enhanced HIF-1<i>α</i> expressions in the rSCC-61 cells. HIF-1<i>α</i> inhibition during the radiation treatment modulates the metabolic changes and radio-sensitizes the rSCC-61 cells. Through these studies, we demonstrated that a standard fluorescence microscope along with proper image processing methods can provide flow cytometry-like single-cell level analysis of HIF-1<i>α</i>-mediated metabolic changes in the radioresistant and radiosensitive HNSCC cells.</p><p><strong>Conclusions: </strong>Our reported optical imaging strategies may enable one to study the role of metabolism reprogramming in cancer therapeutic resistance development at the single-cell level in a more efficient, cost-effective, and non-destructive manner. Our understanding of radiation resistance mechanisms using our imaging methods will offer opportunities to design targeted radiotherapy for improved treatment outcomes for HNSCC patients.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal, label-free imaging to elucidate radiation resistance mechanisms.","authors":"Irene Georgakoudi","doi":"10.1117/1.BIOS.1.3.030502","DOIUrl":"10.1117/1.BIOS.1.3.030502","url":null,"abstract":"<p><p>The commentary remarks on recent work that demonstrates the power of multimodal, label-free imaging to elucidate radiation resistance mechanisms and provides a useful method for plotting multivariable imaging data, uncovering metabolic heterogeneity bulk analyses would miss.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"1 3","pages":"030502"},"PeriodicalIF":0.0,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen remodeling in murine melanoma therapy response through second-harmonic generation imaging.","authors":"Mihaela Balu","doi":"10.1117/1.BIOS.1.3.030501","DOIUrl":"10.1117/1.BIOS.1.3.030501","url":null,"abstract":"<p><p>The study by Heaton et al. marks a significant advancement in understanding the role of collagen remodeling within the melanoma tumor microenvironment during immunotherapy. Using in vivo second-harmonic generation imaging, the authors quantitatively tracked dynamic changes in collagen morphology in a preclinical melanoma model, revealing a shift toward a healthier phenotype associated with treatment. These findings enhance our understanding of tumor extracellular matrix dynamics and highlight the potential of optical imaging technologies to guide and optimize cancer immunotherapy. This commentary will explore these findings, contextualize them within the broader field of tumor immunology, and discuss their implications for improving immunotherapy strategies in melanoma and other cancers.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"1 3","pages":"030501"},"PeriodicalIF":0.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Publisher's Note: Optical coherence tomography to identify upper airway obstruction sites in an apneic patient.","authors":"Joseph C Jing, Khodayar Goshtasbi, Yong Wang, Jason J Chen, Erica Su, Ellen M Hong, Katelyn D Dilley, Yan Li, Frances B Lazarow, Anthony Chin Loy, David Shamouelian, Said E Elghobashi, Zhongping Chen, Brian J F Wong","doi":"10.1117/1.BIOS.1.3.039802","DOIUrl":"10.1117/1.BIOS.1.3.039802","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1117/1.BIOS.1.3.035002.].</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"1 3","pages":"039802"},"PeriodicalIF":0.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Investigating the relationship between hypoxia, hypoxia-inducible factor 1, and the optical redox ratio in response to radiation therapy.","authors":"Jesse D Ivers, Nagavenkatasai Puvvada, Charles M Quick, Narasimhan Rajaram","doi":"10.1117/1.BIOS.1.3.039801","DOIUrl":"10.1117/1.BIOS.1.3.039801","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1117/1.BIOS.1.1.015003.].</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"1 3","pages":"039801"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}