Crop health最新文献

{"title":"Insect‒microbe symbiosis-based strategies offer a new avenue for the management of insect pests and their transmitted pathogens.","authors":"Chao Lv, Yan-Zhen Huang, Jun-Bo Luan","doi":"10.1007/s44297-024-00038-9","DOIUrl":"10.1007/s44297-024-00038-9","url":null,"abstract":"<p><p>With the continuous growth of global agricultural production, pest control has become a critical factor in ensuring crop health and increasing agricultural output. In view of the safety of food and ecology, the development of more environmentally friendly and sustainable approaches for pest management is desirable. All insects are colonized by microorganisms on the insect cuticle or in the body. These resident microorganisms can promote insect fitness, impact the transmission of plant pathogens, or protect insects against natural enemies and adverse environments. Thus, insect‒microbe symbiosis-based strategies provide a new avenue for the management of insect pests and their transmitted pathogens. This review summarizes developments in the field of pest control approaches based on insect‒microbe symbiosis and proposes future directions. First, we introduce insect symbiotic microorganisms and their functions. This review discusses the application of insect-microbe symbiosis-based pest control strategies, including the application of native or engineered symbionts, the utilization of bioactive substances produced by symbiotic microorganisms, and the development of an insect symbiosis disruption strategy. Despite the great potential of this novel pest-control strategy, many challenges remain, such as the stability of symbiotic bacteria, their environmental adaptability, and their impact on non-target organisms. Finally, the review concludes by suggesting future directions, including improving the targeting specificity of symbiotic bacteria, enhancing their environmental adaptability, and developing integrated pest management strategies that combine this means with others to achieve more sustainable and effective pest control.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"18"},"PeriodicalIF":0.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Agrobacterium-mediated soybean transformation efficiency with an auxiliary solution.","authors":"Luying Chen, Liang Wang, Lei Zhang, Yongguang Li, Shaojie Han","doi":"10.1007/s44297-024-00037-w","DOIUrl":"10.1007/s44297-024-00037-w","url":null,"abstract":"<p><p>Soybean is a crucial source of oil, protein, and biofuel, necessitating efficient transformation systems for advancing research. Agrobacterium-mediated transformation is currently the primary method used in the soybean transformation industry and scientific research. However, the low efficiency and genotype dependency of this technology leave significant room for improvement. This study aimed to enhance soybean transformation efficiency by generating and validating three reporter vectors (ZsGreen, TdTomato, and Ruby) and using Agrobacterium Auxiliary Solution (AAS) containing Silwet L-77 and hormone mixtures. Our findings demonstrate that AAS significantly improves hairy root transformation rates. Specifically, this combination increased total root and cotyledon transformation efficiencies compared to the control. We also found that larger vectors like Ruby reduced transformation efficiency compared to smaller markers like GFP and RFP. Furthermore, AAS slightly reduced the co-transformation rate of two separate vectors compared to single vector transformations. Additionally, AAS enhanced soybean hypocotyl transformation rates across various varieties, consistently increasing positive root and explant efficiencies. Notably, transformation rates varied significantly between varieties, with Forrest differing from Williams 82 and Dongnong 50. This research highlights the importance of auxiliary agents and vector size in optimizing soybean transformation, providing insights for future advancements in genetic modification and biotechnology.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The secreted protein FonCHRD is essential for vegetative growth, asexual reproduction, and pathogenicity in watermelon Fusarium wilt fungus.","authors":"Jiajun Lou, Jiajing Wang, Shanshan Zeng, Xiaohui Xiong, Mengmeng Guo, Dayong Li, Fengming Song","doi":"10.1007/s44297-024-00036-x","DOIUrl":"10.1007/s44297-024-00036-x","url":null,"abstract":"<p><p>Fungal pathogens often secrete numerous effectors to interfere with and/or suppress plant immunity to promote their infection. Watermelon Fusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f. sp. niveum (Fon), is one of the devastating diseases that severely affect the watermelon industry. Here, we report the function of a candidate effector protein, FonCHRD, in Fon. FonCHRD harbors a chordin (CHRD) domain of unknown function and has a signal peptide with secretion activity. FonCHRD shows a relatively high expression level in Fon marcoconidia and is inducible by watermelon root tissues. Phenotypic analysis of the targeted deletion mutant revealed that FonCHRD plays roles in vegetative growth, asexual reproduction, and conidial morphology of Fon, while it is not involved in spore germination as well as cell wall, oxidative and salt stress responses. Deletion of FonCHRD impaired the ability to colonize and spread within host plants, significantly reducing its virulence on watermelon. FonCHRD is distributed across multiple compartments of plant cells but can target to the apoplast space in plants. FonCHRD inhibits the INF1- and Bcl2-associated X protein-triggered cell death and defense gene expression in transiently expressed Nicotiana benthamiana leaves. These findings suggest that FonCHRD is essential for Fon pathogenicity by modulating invasive growth and spreading abilities as well as by suppressing plant immune responses.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"16"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mating disruption of the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae) on greenhouse tomatoes.","authors":"Junxia Huang, En Wu, Chunxi Yang, Xiangyu Han, Jinghang Zhang, Mengyu Cao, Fengzhi Deng, Qianshuang Guo, Yongjun Du","doi":"10.1007/s44297-024-00035-y","DOIUrl":"10.1007/s44297-024-00035-y","url":null,"abstract":"<p><p>Mating disruption is an important component of the integrated management system for the tomato leaf miner Tuta absoluta (Meyrick). This study showed that the dosage in the tubing dispensers is closely related to their attractiveness. E3Z8Z11-14:Ac and the binary mixture of E3Z8Z11-14:Ac and E3Z8-14:Ac at a dosage of 80 mg and a placement density of 900 polyethylene (PE) tube dispensers/ha significantly reduced the numbers of T. absoluta adults, larvae and damaged tomato leaves. When the adult density was low in the greenhouse, the relationship between the placement density of dispensers (x) and the number of moths caught (y) was y=310.6-1.06x+0.0008x², which is a typical competitive mating disruption. However, when the adult density was high, the regression equation was y=-1.112x+959.4. Both passive and active dispensers significantly reduced the damage rate and larval population, but the 900 tube dispensers/ha and active aerosol dispensers had the best control results. The effects of the competitive passive dispensers and the placement density of dispensers were influenced by the adult density. At high density, 900 tube dispensers/ha were required.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel highly antifungal compound ZJS-178 targeting myosin I inhibits the endocytosis and mycotoxin biosynthesis of Fusarium graminearum.","authors":"Qiaowan Chen, Chang Liu, Hao Qi, Ningjie Wu, Zunyong Liu, Qin Tian, Xiao-Xiao Zhu, Xiangdong Li, Yun Chen, Zhonghua Ma","doi":"10.1007/s44297-024-00034-z","DOIUrl":"10.1007/s44297-024-00034-z","url":null,"abstract":"<p><p>Chemical fungicides remain a primary tool for managing Fusarium head blight (FHB) due to the lack of resistant wheat cultivars. In this study, we synthesized 101 2-cyanoacrylate compounds and identified ZJS-178 as particularly effective against Fusarium graminearum (the causal agent of FHB), inhibiting both hyphal growth and conidial germination. Notably, ZJS-178 is specific to Fusarium species, with no impact on other fungi. It also shows no cross-resistance with other groups of fungicides, suggesting a unique mode of action. Mechanistic studies revealed that ZJS-178 targets myosin I in F. graminearum, inhibiting ATPase activity and disrupting endocytosis. Furthermore, ZJS-178 impairs toxisome formation, leading to reduced production of the mycotoxin deoxynivalenol (DON). These findings position ZJS-178 as a promising antifungal agent that could pave the way for developing a novel fungicide to manage FHB and other Fusarium-related diseases.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of a novel whitefly- and aphid-transmitted polerovirus on rice plants with dwarfing and fewer tillering symptoms.","authors":"Jiaping Yu, Ming Zeng, Yali Zhou, Jirui Wang, Xueping Zhou, Jianxiang Wu","doi":"10.1007/s44297-024-00033-0","DOIUrl":"10.1007/s44297-024-00033-0","url":null,"abstract":"<p><p>Rice is the most important cereal grain worldwide and is cultivated mainly in Asian countries. Rice viral disease epidemics caused by insect-borne viruses are a major threat to rice production. However, to date, how many unknown viruses naturally infect rice plants in the field remains vague. Here, we identified a novel polerovirus tentatively named rice dwarf polerovirus (RDPV) via RNA-seq technology in rice plants showing dwarfing and fewer tillering symptoms collected from paddy fields in Hainan Province, China. The RDPV genome consists of a positive-sense, single-stranded RNA molecule that is 5832 nt in size and is deduced to encode seven viral proteins. Phylogenetic analyses based on the full-length genome sequence and RNA-dependent RNA polymerase (RdRp) amino acid sequence revealed that RDPV is in an evolutionary branch with known viruses in the Polerovirus genus. Interestingly, virus transmission experiments demonstrated that RDPV can be transmitted by two completely different vectors, i.e., whiteflies and aphids, from diseased rice plants to healthy rice seedlings. Furthermore, we constructed an infectious cDNA clone of RDPV and found that it can infect Nicotiana benthamiana plants via Agrobacterium-infiltrated inoculation. Thus, we concluded that RDPV is a novel whitefly- and aphid-transmitted rice polerovirus. These findings contribute to expanding our knowledge of rice polerovirus diversity, and developing efficient strategies to manage this rice viral disease.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"13"},"PeriodicalIF":0.0,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The discovery of a new lifespan-extending gene in insects","authors":"Jacob L. Steenwyk","doi":"10.1007/s44297-024-00032-1","DOIUrl":"https://doi.org/10.1007/s44297-024-00032-1","url":null,"abstract":"","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"80 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141810270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose-montmorillonite hydrochar composite activating peroxymonosulfate for sulfentrazone rapid degradation and phytotoxicity alleviation to rice","authors":"Huan Yi, Guanghua Mo, Xuguo Zhou, A. Merchant, Hailin Cai, Yaping Tao, Kailin Liu, Guolan Ma, Chunxia Ding, Xiangying Liu","doi":"10.1007/s44297-024-00031-2","DOIUrl":"https://doi.org/10.1007/s44297-024-00031-2","url":null,"abstract":"","PeriodicalId":519973,"journal":{"name":"Crop health","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141675115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prostaglandin A3 regulates the colony development of <i>Odontotermes formosanus</i> by reducing worker proportion.","authors":"Qihuan Zhou, Ting Yu, Wuhan Li, Raghda Nasser, Nooney Chidwala, Jianchu Mo","doi":"10.1007/s44297-024-00030-3","DOIUrl":"10.1007/s44297-024-00030-3","url":null,"abstract":"<p><p>Subterranean termites cause significant economic losses worldwide due to their destruction of agricultural and forest plants. In the past, soil termiticides were commonly used to control subterranean termites because they were effective and affordable. However, due to growing environmental concerns, these harmful substances have become less popular as they cause damage to non-target organisms and lead to environmental contamination. Baits crafted from plants and other easily metabolized compounds serve as excellent alternatives. In this study, we gathered branches from the promising plant, <i>Magnolia grandiflora</i> L. (MGL), along with branches from five other tree species that are potential food for termites. These branches were used as food to observe the population growth of <i>Odontotermes formosanus</i>. Additionally, a mix of branches from all six species was used to feed the control group (MIX). The study results showed that MGL nutrition significantly inhibited worker development, resulting in a significantly lower worker-to-soldier ratio (WSR). Furthermore, LC‒MS/MS analysis revealed that the level of prostaglandin A3 (PGA₃) in workers significantly increased when they were under MGL nutrition. Additionally, ICP-MS analysis indicated a significant increase in calcium concentrations in the branches of MGL and combs under MGL nutrition. Moreover, there was a significant increase in peroxidase (POD) activity in workers under MGL nutrition. These findings suggest that the inhibitory effect of MGL nutrition on worker development may be due to excessive PGA₃ synthesis, as Ca²⁺ and POD are involved in the synthesis process of PGs in insects. Subsequent verification experiments strongly support this hypothesis, as the WSR of colonies fed PGA₃-added MIX was significantly lower than that of the MIX alone. This study introduces a new concept for developing environmentally friendly biological control methods for <i>O. formosanus</i> and sheds light on the potential role of PGs in termite development.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s44297-024-00030-3.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"2 1","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional, eco-friendly, and starch-based nanocarriers with sustained release of carvacrol for persistent control of tomato gray mold.","authors":"Wenxuan Shang, Qiuyu Xiong, Zhengang Xie, Jingli Cheng, Bin Yu, Haonan Zhang, Yehua Su, Jinhao Zhao","doi":"10.1007/s44297-023-00014-9","DOIUrl":"10.1007/s44297-023-00014-9","url":null,"abstract":"<p><p>Carvacrol (Car) is a promising botanical fungicide. However, the volatile and easily oxidizable characterization of Car hinders its practical application. Herein, an eco-friendly, starch-based nanopesticide (Car@ACS) was successfully constructed by encapsulating of Car within acetylated corn starch, enabling sustained release to prolong its efficacy. In vitro release experiments showed that the release of Car from commercial carvacrol soluble concentrate (Car SL) was 98.4% within 4 h, while that of Car@ACS released 36% within 4 h and 79% within 48 h, demonstrating the endowed sustained release property of the starch nanoparticles. Meanwhile, in vitro antimicrobial activity experiments revealed the fungicidal ability of the prepared Car@ACS and carvacrol technical (Car Tech) with the same concentration of active ingredient to be comparable Botrytis cinerea. In the 7-day pot experiment, the efficacy of the Car@ACS group (56.9%) against B. cinerea was significantly greater than that of the Car SL group (33.3%). In addition, the Car@ACS formulation improved the foliar retention of Car compared to Car SL in hydrophilic cucumber and hydrophobic peanut leaves. Finally, biosafety tests indicated that Car@ACS did not significantly affect the germination or growth of tomato seedlings. Therefore, the advantages of long-lasting disease control properties, improved foliar retention and eco-friendliness indicate that Car@ACS has significant potential to facilitate the application of volatile botanical fungicides.</p>","PeriodicalId":519973,"journal":{"name":"Crop health","volume":"1 1","pages":"13"},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}