bioRxiv : the preprint server for biology最新文献_第9页

Proline 110 is necessary for maintaining a compact helical arrangement in caveolin-1. 脯氨酸110是维持小洞蛋白-1紧密螺旋排列所必需的。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.10.664188

Katrina Brandmier, Kerney Jebrell Glover

{"title":"Proline 110 is necessary for maintaining a compact helical arrangement in caveolin-1.","authors":"Katrina Brandmier, Kerney Jebrell Glover","doi":"10.1101/2025.07.10.664188","DOIUrl":"https://doi.org/10.1101/2025.07.10.664188","url":null,"abstract":"Caveolin-1 (Cav1) is an integral membrane protein essential for the formation of caveolae, plasma microdomains implicated in signal transduction and mechanoprotection. Cav1 is comprised of three major alpha helices, but the topology these helices adopt remains unclear. Proline 110 is located between helix 1 and helix 2, and is hypothesized to enable Cav1 to adopt an intramembrane turn crucial for the cytosolic topology of Cav1. To assess the structural role of Proline 110, we utilized Förster resonance energy transfer (FRET) between native tryptophan (W128) and site-specifically labeled dansyl fluorophores to monitor conformational changes induced by the mutation of Proline 110 to Alanine (P110A). Static light scattering confirmed that all FRET constructs behaved monomerically, ensuring intramolecular energy transfer measurements. Our results show a significant decrease in FRET efficiency upon the P110A mutation consistent with a large conformational change. These findings support the critical role of P110 in maintaining the native topology of Cav1 and highlights the structural sensitivity of the intramembrane turn.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dimerization and dynamics of angiotensin-I converting enzyme revealed by cryo-EM and MD simulations. 血管紧张素- 1转化酶的二聚化和动力学研究。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.01.09.632263

Jordan M Mancl, Xiaoyang Wu, Minglei Zhao, Wei-Jen Tang

{"title":"Dimerization and dynamics of angiotensin-I converting enzyme revealed by cryo-EM and MD simulations.","authors":"Jordan M Mancl, Xiaoyang Wu, Minglei Zhao, Wei-Jen Tang","doi":"10.1101/2025.01.09.632263","DOIUrl":"10.1101/2025.01.09.632263","url":null,"abstract":"Angiotensin-I converting enzyme (ACE) regulates the levels of disparate bioactive peptides, notably converting angiotensin-I to angiotensin-II and degrading amyloid beta. ACE is a heavily glycosylated dimer, containing 4 analogous catalytic sites, and exists in membrane bound and soluble (sACE) forms. ACE inhibition is a frontline, FDA-approved, therapy for cardiovascular diseases yet is associated with significant side effects, including higher rates of lung cancer. To date, structural studies have been confined to individual domains or partially denatured cryo-EM structures. Here we report the cryo-EM structure of the glycosylated full sACE dimer. We resolved four structural states at 2.99 to 3.65 Å resolution which are primarily differentiated by varying degrees of solvent accessibility to the active sites and reveal the full dimerization interface. We also employed all-atom molecular dynamics (MD) simulations and heterogeneity analysis in cryoSPARC, cryoDRGN, and RECOVAR to elucidate the conformational dynamics of sACE and identify key regions mediating conformational change. We identify differences in the mechanisms governing the conformational dynamics of individual domains that have implications for the design of domain-specific sACE modulators.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-activity Relationship for Diarylpyrazoles as Inhibitors of the Fungal Kinase Yck2. 二芳基吡唑类真菌激酶Yck2抑制剂的构效关系

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.12.664496

David J Shirley, Bonnie Yiu, Ikeer Mancera-Ortiz, Peter J Stogios, Zhongle Liu, Nicole Robbins, Luke Whitesell, Leah E Cowen, David H Drewry, Timothy M Willson

{"title":"Structure-activity Relationship for Diarylpyrazoles as Inhibitors of the Fungal Kinase Yck2.","authors":"David J Shirley, Bonnie Yiu, Ikeer Mancera-Ortiz, Peter J Stogios, Zhongle Liu, Nicole Robbins, Luke Whitesell, Leah E Cowen, David H Drewry, Timothy M Willson","doi":"10.1101/2025.07.12.664496","DOIUrl":"10.1101/2025.07.12.664496","url":null,"abstract":"Candida albicans is a growing global health threat, causing 1.5 million invasive infections and 1 million deaths annually. Yeast casein kinase 2 (Yck2) in C. albicans has emerged as an antifungal target of the kinase inhibitor LY364947 (LY). Herein, we report Yck2 structure-activity relationships for 3,4- and 3,4,5-substituted pyrazole analogs of LY. X-ray crystallography and in vitro profiling revealed the importance of the hinge-binding heterocycle for Yck2 inhibition and fungal kinome selectivity. A hydrogen-bond network between the inhibitor, a bound water molecule, and catalytic residues within the ATP pocket was identified as a key determinant of selectivity over other fungal and human kinases. Phenol analog 11 showed remarkable selectivity for Yck2 and Yck22 over all other C. albicans protein kinases. Several of the LY analogs, including 11, demonstrated improved antifungal activity. These findings provide a framework for translating human kinase inhibitors into highly selective antifungal Yck2 inhibitors.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of FMRP in microglia promotes degeneration of parvalbumin neurons and audiogenic seizures via progranulin insufficiency. 小胶质细胞中FMRP的缺失可促进小白蛋白神经元变性和通过蛋白前蛋白不足引起的听原性癫痫发作。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.09.663876

Heng Li, Jennifer Ja-Yoon Choi, Eric J Huang, Baoji Xu

{"title":"Loss of FMRP in microglia promotes degeneration of parvalbumin neurons and audiogenic seizures via progranulin insufficiency.","authors":"Heng Li, Jennifer Ja-Yoon Choi, Eric J Huang, Baoji Xu","doi":"10.1101/2025.07.09.663876","DOIUrl":"https://doi.org/10.1101/2025.07.09.663876","url":null,"abstract":"Fragile X syndrome (FXS) results from loss of FMR1-encoded FMRP and is associated with reduced density of parvalbumin (PV) neurons; however, the mechanism underlying this abnormality remains unknown. Here we report that microglial FMRP regulates PV neuron density through lysosomal function. Mice with Fmr1 deletion in microglia exhibited audiogenic seizures (AGS) and decreased PV neuron density in the cortex and AGS-associated inferior colliculus (IC). FMRP increased the expression of lysosomal genes in microglia, including the progranulin-encoding Grn gene. Its loss in microglia led to impaired lysosomal function and increased apoptosis in microglia and PV neurons. Furthermore, PV neuron density in the IC was reduced similarly in male Grn +/- , Fmr1 -/y , and Grn +/- ; Fmr1 -/y mice, and AAV8-mediated overexpression of progranulin rescued AGS and PV neuron loss in Fmr1 -/y mice. This indicates that progranulin insufficiency is a determinant for PV neuron loss in FXS and elevating progranulin is a therapeutic strategy for FXS.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perineuronal Net and Inhibitory Synapse Remodeling on Striatal Fast-spiking Interneurons by Chronic Alcohol Exposure. 慢性酒精暴露对纹状体快速尖峰中间神经元的神经网络和抑制性突触重塑。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.08.663744

Michael S Patton, Samuel H Sheats, Andreas B Wulff, Paige N McKeon, Jonathan W VanRyzin, Mary H Patton, Morgan Heckman, Allison N Siclair, Phillip H Iffland, Brian N Mathur

{"title":"Perineuronal Net and Inhibitory Synapse Remodeling on Striatal Fast-spiking Interneurons by Chronic Alcohol Exposure.","authors":"Michael S Patton, Samuel H Sheats, Andreas B Wulff, Paige N McKeon, Jonathan W VanRyzin, Mary H Patton, Morgan Heckman, Allison N Siclair, Phillip H Iffland, Brian N Mathur","doi":"10.1101/2025.07.08.663744","DOIUrl":"https://doi.org/10.1101/2025.07.08.663744","url":null,"abstract":"Alcohol use disorder is characterized by persistent drinking in the face of negative consequences. Such inflexible drinking requires dorsolateral striatum fast-spiking interneurons, which comprise roughly 1% of all striatal neurons. How chronic ethanol exposure affects fast-spiking interneuron physiology is poorly understood. We discover in mice that chronic ethanol exposure induced a dramatic loss of GABAergic, but not glutamatergic, synapses onto dorsolateral striatum fast-spiking interneuron somata and proximal dendrites where perineuronal nets, a subdivision of the extracellular matrix, are enriched. We found that chronic ethanol exposure degraded these perineuronal nets and that enzymatically degrading perineuronal nets similarly reduced GABAergic transmission onto dorsolateral striatum fast-spiking interneurons. Modeling the effect of alcohol, we find that silencing extrinsic GABAergic projections to the dorsolateral striatum increased voluntary ethanol consumption. Taken together, these data suggest chronic alcohol exposure remodels perineuronal nets and inhibitory synapses on fast-spiking interneurons to facilitate alcohol drinking.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular basis for cortical network aging in primates. 灵长类动物皮层网络老化的细胞基础。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.08.663725

Melina Tsotras, Joey A Charbonneau, Claude Lepage, Jeffrey L Bennett, Jelle Veraart, Alan C Evans, Eliza Bliss-Moreau, Erika P Raven

{"title":"Cellular basis for cortical network aging in primates.","authors":"Melina Tsotras, Joey A Charbonneau, Claude Lepage, Jeffrey L Bennett, Jelle Veraart, Alan C Evans, Eliza Bliss-Moreau, Erika P Raven","doi":"10.1101/2025.07.08.663725","DOIUrl":"10.1101/2025.07.08.663725","url":null,"abstract":"Large-scale brain networks are vulnerable to change with aging and become dysregulated. How these networks are altered at the cellular level remains unclear owing to challenges of bridging data across scales. Here, we integrate in vivo cortical similarity networks with whole brain spatial transcriptomics to characterize the aging brain in a lifespan cohort of macaques (N=64, ages 1-26 years). Deep-layer excitatory neurons and oligodendrocytes emerged as dominant correlates of cortical similarity, linking infragranular cell type composition to macroscopic network structure. Age-related declines in network strength were most pronounced in transmodal networks, including default mode and limbic, and aligned with regions enriched in inhibitory and glial cell types. Parvalbumin-enriched chandelier cells showed the strongest association with regional vulnerability, suggesting a role in network disconnection. Cell-type enrichment was conserved across species, with both human and macaque transcriptomic data aligning with the cortical functional hierarchy. These findings uncover a cellular basis for cortical network aging and highlight the value of imaging-transcriptomic integration across scales.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel microdroplet based glioblastoma drug screening platform. 基于水凝胶微滴的胶质母细胞瘤药物筛选平台。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.08.663758

Brittany A Payan, Annika Carrillo Diaz De Leon, Tejasvi Anand, Gunnar B Thompson, Ana Mora-Boza, Vishnu V Krishnamurthy, Andrés J García, Brendan A C Harley

{"title":"Hydrogel microdroplet based glioblastoma drug screening platform.","authors":"Brittany A Payan, Annika Carrillo Diaz De Leon, Tejasvi Anand, Gunnar B Thompson, Ana Mora-Boza, Vishnu V Krishnamurthy, Andrés J García, Brendan A C Harley","doi":"10.1101/2025.07.08.663758","DOIUrl":"10.1101/2025.07.08.663758","url":null,"abstract":"Glioblastoma is the most common primary malignant brain tumor with a five-year survival rate less than 5%. The standard of care involves surgical resection followed by treatment with the alkylating agent temozolomide (TMZ). GBM cells that evade surgery eventually become resistant to TMZ and lead to recurrence of tumors in patients. With only four drugs currently FDA-approved for GBM treatment, there is a need for a clinically relevant model capable of accelerating the identification of new therapies. Microgels are microscale (~10-1,000 μm) hydrogel particles that can be used to encapsulate cells in a tailorable 3D matrix. Microdroplets offer short diffusion lengths relative to conventional hydrogel constructs (>1 mm) to limit spatial distributions of hypoxia and potentially screen therapeutics in a controlled and physiologically relevant environment. Here, we establish a method to encapsulate GBM cells in gelatin and polyethylene glycol (PEG) microgels. We show that microgel composition can affect cell morphology and further, that collections of GBM-laden hydrogels can be used to quantify the effect of single vs. metronomic doses of TMZ. GBM metabolic activity is maintained in microgel culture and GBM cells display drug response kinetics similar to previously established literature using macro-scale hydrogel constructs. Finally, we show microgels can be integrated with a liquid handler to enable high-throughput screening using cell-laden microgels.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRIMPOL promotes replication fork progression but not double strand break formation in FBH1-deficient cells in response to hydroxyurea. PRIMPOL促进fbh1缺陷细胞对羟基脲的复制叉进展，但不促进双链断裂的形成。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.08.663736

Joshua L Turner, Georgia Moore, Jennifer M Mason

引用次数: 0

Spatially resolved mapping of monoacylglycerol lipase activity in the brain. 脑内单酰基甘油脂肪酶活性的空间分辨图谱。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.08.663730

Daan van der Vliet, Alex X Y Klinkenberg, Rik Platte, Kieran Higgins, Susanne Prokop, Mirjam C W Huizenga, Lars Kraaijevanger, Noëlle van Egmond, Verena M Straub, Maarten H P Kole, Pal Pacher, István Katona, Inge Huitinga, Mario van der Stelt

{"title":"Spatially resolved mapping of monoacylglycerol lipase activity in the brain.","authors":"Daan van der Vliet, Alex X Y Klinkenberg, Rik Platte, Kieran Higgins, Susanne Prokop, Mirjam C W Huizenga, Lars Kraaijevanger, Noëlle van Egmond, Verena M Straub, Maarten H P Kole, Pal Pacher, István Katona, Inge Huitinga, Mario van der Stelt","doi":"10.1101/2025.07.08.663730","DOIUrl":"https://doi.org/10.1101/2025.07.08.663730","url":null,"abstract":"Visualizing signaling systems in the brain with high spatial resolution is critical to understand brain function and to develop therapeutics. Especially enzymes are often regulated on the post-translational level, resulting in a disconnect between protein levels and activity. Conventional antibody-based methods have limitations, including potential cross reactivity and the inability of antibodies to discriminate between active and inactive enzyme states. Monoacylglycerol lipase (MAGL), an enzyme degrading the neuroprotective endocannabinoid 2-arachidonoylglycerol, is the target of inhibitors currently in clinical trials for the treatment of several neurological disorders. To support translational and (pre)clinical studies and fully realize the therapeutic opportunities of MAGL inhibitors, it is essential to map the spatial distribution of MAGL activity throughout the brain in both health and disease. Here, we introduce selective fluorescent activity-based probes for MAGL enabling direct visualization of its enzymatic activity in lysates, cultured cells and tissue sections. We show that oxidative stress, which inactivates MAGL through the oxidation of regulatory cysteines, reduces probe labeling, thereby validating the probes activity-dependence. Extending this approach, we developed an activity-based histology protocol to visualize MAGL activity in fresh-frozen mouse and human brain tissues. This approach revealed robust MAGL activity in astrocytes and presynaptic terminals within the mouse hippocampus, and further allows detection of MAGL activity in the human cerebral cortex. Collectively, these findings establish selective activity-based probes as powerful tools mapping MAGL activity with high spatial resolution across mammalian brain tissue.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural modeling reveals viral proteins that manipulate host immune signaling. 结构建模揭示了操纵宿主免疫信号的病毒蛋白。

bioRxiv : the preprint server for biology Pub Date : 2025-07-12 DOI: 10.1101/2025.07.12.664507

Nitzan Tal, Romi Hadari, Renee B Chang, Ilya Osterman, Roy Jacobson, Erez Yirmiya, Nathalie Bechon, Dina Hochhauser, Miguel López Rivera, Barak Madhala, Jeremy Garb, Tanita Wein, Philip Kranzusch, Gil Amitai, Rotem Sorek

{"title":"Structural modeling reveals viral proteins that manipulate host immune signaling.","authors":"Nitzan Tal, Romi Hadari, Renee B Chang, Ilya Osterman, Roy Jacobson, Erez Yirmiya, Nathalie Bechon, Dina Hochhauser, Miguel López Rivera, Barak Madhala, Jeremy Garb, Tanita Wein, Philip Kranzusch, Gil Amitai, Rotem Sorek","doi":"10.1101/2025.07.12.664507","DOIUrl":"https://doi.org/10.1101/2025.07.12.664507","url":null,"abstract":"Immune pathways that use intracellular nucleotide signaling are common in animals, plants and bacteria. Viruses can inhibit nucleotide immune signaling by producing proteins that sequester or cleave the immune signals. Here we analyzed evolutionarily unrelated signal-sequestering viral proteins, finding that they share structural and biophysical traits in their genetic organization, ternary structures and binding pocket properties. Based on these traits we developed a structure-guided computational pipeline that can sift through large phage genome databases to unbiasedly predict phage proteins that manipulate bacterial immune signaling. Numerous previously uncharacterized proteins, grouped into three families, were verified to inhibit the bacterial Thoeris and CBASS signaling systems. Proteins of the Sequestin and Lockin families bind and sequester the TIR-produced signaling molecules 3'cADPR and His-ADPR, while proteins of the Acb5 family cleave and inactivate 3'3'-cGAMP and related molecules. X-ray crystallography and structural modeling, combined with mutational analyses, explain the structural basis for sequestration or cleavage of the immune signals. Thousands of these signal-manipulating proteins were detected in phage protein databases, with some instances present in well-studied model phages such as T2, T4 and T6. Our study explains how phages commonly evade bacterial immune signaling, and offers a structure-guided analytical approach for discovery of viral immune-manipulating proteins in any database of choice.","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0