Journal of physiological investigation最新文献_第6页

The Effect of High- and Low-power Holmium Laser Settings for Transurethral Lithotripsy in the Management of Adults with Ureteral Stone. 经尿道碎石术中高功率和低功率钬激光设置对治疗成人输尿管结石的效果。

Journal of physiological investigation Pub Date : 2024-05-01 Epub Date: 2024-06-21 DOI: 10.4103/ejpi.EJPI-D-24-00027

Yuhuan Wang, Jun Wang, Xiaoping Sun

{"title":"The Effect of High- and Low-power Holmium Laser Settings for Transurethral Lithotripsy in the Management of Adults with Ureteral Stone.","authors":"Yuhuan Wang, Jun Wang, Xiaoping Sun","doi":"10.4103/ejpi.EJPI-D-24-00027","DOIUrl":"10.4103/ejpi.EJPI-D-24-00027","url":null,"abstract":"Since there is insufficient evidence to determine the best treatment of transurethral laser lithotripsy (TLL) in ureteral stones, this study compared the effectiveness and safety of TLL using high-power (HP) (100 W) and low-power (LP) (20 W) laser settings. All patients with maximally sized ureteral stones who were planned for transurethral holmium laser lithotripsy were enrolled in this open study. One of the two laser setting groups-LP or HP-was allocated to each alternate patient. Using IBM SPSS Statistics 24, the treatment groups were compared for operating time, intraoperative and postoperative problems (up to 1 year), and rates of stone-free recovery. Welch tests were employed to compare continuous data, whereas Fisher's exact or Chi-square tests were used to assess categorical variables. At P < 0.05, statistical significance was established. A total of 207 individuals were included and preoperative data were comparable between the two groups. The HP group had a considerably greater ablation rate and a significantly shorter procedure duration (42.61 ± 11.74 min) than the LP group (78.56 ± 25.91 min) ( P = 0.025). The Overactive Bladder Symptom Score and International Prostate Symptom Score were considerably higher in the HP group than in the LP group. Treatment effectiveness was considerably impacted by the location of the ureteral stone, according to univariate and multivariate logistic regression models. A HP laser setting of up to 100 W greatly shortens the duration of the process for treating ureteral stones without raising the risk of problems.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":" ","pages":"153-160"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of miR-204-5p Alleviates Osteogenic Differentiation and Calcification of Human Aortic Vascular Smooth Muscle Cells by Targeting Calcium/Calmodulin-dependent Protein Kinase 1. 通过靶向钙/钙调蛋白依赖性蛋白激酶 1，过表达 miR-204-5p 可减轻人主动脉血管平滑肌细胞的成骨分化和钙化。

Journal of physiological investigation Pub Date : 2024-03-01 Epub Date: 2024-04-30 DOI: 10.4103/ejpi.EJPI-D-24-00003

Chunli Wang, Mingzhi Xu, Yafei Bai, Mingjiao Pan, Yonghui Qi, Ruman Chen

{"title":"Overexpression of miR-204-5p Alleviates Osteogenic Differentiation and Calcification of Human Aortic Vascular Smooth Muscle Cells by Targeting Calcium/Calmodulin-dependent Protein Kinase 1.","authors":"Chunli Wang, Mingzhi Xu, Yafei Bai, Mingjiao Pan, Yonghui Qi, Ruman Chen","doi":"10.4103/ejpi.EJPI-D-24-00003","DOIUrl":"10.4103/ejpi.EJPI-D-24-00003","url":null,"abstract":"Abstract: Vascular calcification (VC), a major complication in chronic kidney disease (CKD), is predominantly driven by osteoblastic differentiation. Recent studies have highlighted the crucial role of microRNAs in CKD's pathogenesis. Here, our research focused on the effects of miR-204-5p and its molecular mechanisms within VC. We initially found a notable decrease in miR-204-5p levels in human aortic vascular smooth muscle cells stimulated with inorganic phosphate, using this as a VC model in vitro. Following the overexpression of miR-204-5p, a decrease in VC was observed, as indicated by alizarin red S staining and measurements of calcium content. This decrease was accompanied by lower levels of the osteogenic marker, runt-related transcription factor 2, and higher levels of α-smooth muscle actin, a marker of contractility. Further investigation showed that calcium/calmodulin-dependent protein kinase 1 (CAMK1), which is a predicted target of miR-204-5p, promotes VC. Conversely, overexpressing miR-204-5p reduced VC by suppressing CAMK1 activity. Overexpressing miR-204-5p also effectively mitigated aortic calcification in an in vivo rat model. In summary, our research indicated that targeting the miR-204-5p/CAMK1 pathway could be a viable strategy for mitigating VC in CKD patients.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":"67 2","pages":"69-78"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between Uterine Adenomyosis and Infertility: Role of Axonemal Alteration in Apical Endometria. 子宫腺肌症与不孕之间的关系：子宫内膜顶端轴索改变的作用

Journal of physiological investigation Pub Date : 2024-03-01 Epub Date: 2024-04-30 DOI: 10.4103/ejpi.EJPI-D-24-00032

Khaleque N Khan

{"title":"Association between Uterine Adenomyosis and Infertility: Role of Axonemal Alteration in Apical Endometria.","authors":"Khaleque N Khan","doi":"10.4103/ejpi.EJPI-D-24-00032","DOIUrl":"10.4103/ejpi.EJPI-D-24-00032","url":null,"abstract":"Abstract: Uterine adenomyosis is an estrogen-dependent chronic inflammatory condition and may cause painful symptoms, abnormal uterine bleeding, and/or subfertility/infertility. It is characterized by the presence of endometrial glands and stroma within the myometrium causing enlargement of the uterus as a result of reactive hyperplastic and/or hypertrophic change of the surrounding myometrium. Similar to endometriosis, adenomyosis has a negative impact on female fertility. Abnormal uterotubal sperm transport, tissue inflammation, and the toxic effect of chemical mediators have been proposed as contributing factors. Inflammation-induced damage of the mucosal cilia in the fallopian tube has been reported. Besides other proposed mechanisms, our most recent study with transmission electron microscopy analysis indicated that microvilli damage and an axonemal alteration in the apical endometria occur in response to endometrial inflammation. This may be involved in the negative fertility outcome in women with adenomyosis. We present a critical analysis of the literature data concerning the mechanistic basis of infertility in women with adenomyosis and its impact on fertility outcome.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":"67 2","pages":"57-63"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Buyang Huanwu Decoction Alleviates Atherosclerosis by Regulating gut Microbiome and Metabolites in Apolipoprotein E-deficient Mice fed with High-fat Diet. 步阳黄酒煎剂通过调节高脂饮食载脂蛋白 E 缺乏小鼠的肠道微生物组和代谢物缓解动脉粥样硬化

Journal of physiological investigation Pub Date : 2024-03-01 Epub Date: 2024-04-30 DOI: 10.4103/ejpi.EJPI-D-23-00031

Qun Yu, Yilin Zhang, Wenyun Zeng, Yingxin Sun, Xiaolu Zhang, Lin Guo, Yue Zhang, Bin Yu, Maojuan Guo, Yu Wang, Huhu Li, Yanrong Suo, Xijuan Jiang, Lili Song

{"title":"Buyang Huanwu Decoction Alleviates Atherosclerosis by Regulating gut Microbiome and Metabolites in Apolipoprotein E-deficient Mice fed with High-fat Diet.","authors":"Qun Yu, Yilin Zhang, Wenyun Zeng, Yingxin Sun, Xiaolu Zhang, Lin Guo, Yue Zhang, Bin Yu, Maojuan Guo, Yu Wang, Huhu Li, Yanrong Suo, Xijuan Jiang, Lili Song","doi":"10.4103/ejpi.EJPI-D-23-00031","DOIUrl":"10.4103/ejpi.EJPI-D-23-00031","url":null,"abstract":"Abstract: The traditional Chinese herbal prescription Buyang Huanwu decoction (BHD), effectively treats atherosclerosis. However, the mechanism of BHD in atherosclerosis remains unclear. We aimed to determine whether BHD could alleviate atherosclerosis by altering the microbiome-associated metabolic changes in atherosclerotic mice. An atherosclerotic model was established in apolipoprotein E-deficient mice fed high-fat diet, and BHD was administered through gavage for 12 weeks at 8.4 g/kg/d and 16.8 g/kg/d. The atherosclerotic plaque size, composition, serum lipid profile, and inflammatory cytokines, were assessed. Mechanistically, metabolomic and microbiota profiles were analyzed by liquid chromatography-mass spectrometry and 16S rRNA gene sequencing, respectively. Furthermore, intestinal microbiota and atherosclerosis-related metabolic parameters were correlated using Spearman analysis. Atherosclerotic mice treated with BHD exhibited reduced plaque area, aortic lumen occlusion, and lipid accumulation in the aortic root. Nine perturbed serum metabolites were significantly restored along with the relative abundance of microbiota at the family and genus levels but not at the phylum level. Gut microbiome improvement was strongly negatively correlated with improved metabolite levels. BHD treatment effectively slows the progression of atherosclerosis by regulating altered intestinal microbiota and perturbed metabolites.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":"67 2","pages":"88-102"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between Regular Exercise and Urinary Uric Acid Excretion in Chinese Adults: A Cross-sectional Study. 中国成年人经常运动与尿酸排泄之间的关系：一项横断面研究

Journal of physiological investigation Pub Date : 2024-03-01 Epub Date: 2024-04-30 DOI: 10.4103/ejpi.EJPI-D-24-00001

Wenjuan Wang, Xiaoying Zhou, Yu Liu, Miaomiao Sang, Zilin Sun, Shanhu Qiu

{"title":"Association between Regular Exercise and Urinary Uric Acid Excretion in Chinese Adults: A Cross-sectional Study.","authors":"Wenjuan Wang, Xiaoying Zhou, Yu Liu, Miaomiao Sang, Zilin Sun, Shanhu Qiu","doi":"10.4103/ejpi.EJPI-D-24-00001","DOIUrl":"10.4103/ejpi.EJPI-D-24-00001","url":null,"abstract":"Abstract: Regular exercise decreases the risk of hyperuricemia, which is related to reduced urinary uric acid excretion (UUA/UAE). However, few studies have assessed the association between regular exercise and UAE. We conducted this study to investigate their associations in the general population. This was a cross-sectional study that involved a total of 5513 Chinese community-dwellers, who reported data on physical exercise. Fasting morning spot urine was used to measure UUA and creatinine. UAE was primarily assessed by UUA to creatinine ratio (UCr) and secondly by excretion of uric acid per volume of glomerular filtration (EurGF) and fractional excretion of uric acid (FEua). Linear regression analysis was used to assess their associations. Of the included participants, their mean age was 55.1 ± 13.0 years, and 1984 (36.0%) undertook regular exercise. Compared with participants with regular exercise, those with irregular exercise or no exercise had higher UUA/UCr (0.36 ± 0.15 vs. 0.39 ± 0.15, P < 0.001), EurGF (0.23 ± 0.10 vs. 0.24 ± 0.11 mg/dL, P < 0.001), and FEua (0.07 ± 0.04 vs. 0.08 ± 0.04, P = 0.006). These associations remained significant after controlling for multivariable including serum uric acid (SUA) or upon the exclusion of participants with renal dysfunction or urinary tract infection. In conclusion, regular exercise might be associated with decreased UAE, independent of SUA, in Chinese community-dwellers.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":"67 2","pages":"64-68"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Central Oxytocin in Autonomic Regulation. 中枢催产素在自主神经调节中的作用。

Journal of physiological investigation Pub Date : 2024-01-01 Epub Date: 2024-02-28 DOI: 10.4103/EJPI.EJPI-D-23-00037

Sheng-Feng Tsai, Yu-Min Kuo

{"title":"The Role of Central Oxytocin in Autonomic Regulation.","authors":"Sheng-Feng Tsai, Yu-Min Kuo","doi":"10.4103/EJPI.EJPI-D-23-00037","DOIUrl":"10.4103/EJPI.EJPI-D-23-00037","url":null,"abstract":"Oxytocin (OXT), a neuropeptide originating from the hypothalamus and traditionally associated with peripheral functions in parturition and lactation, has emerged as a pivotal player in the central regulation of the autonomic nervous system (ANS). This comprehensive ANS, comprising sympathetic, parasympathetic, and enteric components, intricately combines sympathetic and parasympathetic influences to provide unified control. The central oversight of sympathetic and parasympathetic outputs involves a network of interconnected regions spanning the neuroaxis, playing a pivotal role in the real-time regulation of visceral function, homeostasis, and adaptation to challenges. This review unveils the significant involvement of the central OXT system in modulating autonomic functions, shedding light on diverse subpopulations of OXT neurons within the paraventricular nucleus of the hypothalamus and their intricate projections. The narrative progresses from the basics of central ANS regulation to a detailed discussion of the central controls of sympathetic and parasympathetic outflows. The subsequent segment focuses specifically on the central OXT system, providing a foundation for exploring the central role of OXT in ANS regulation. This review synthesizes current knowledge, paving the way for future research endeavors to unravel the full scope of autonomic control and understand multifaceted impact of OXT on physiological outcomes.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":"67 1","pages":"3-14"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty Acid Binding Protein-4 Silencing Inhibits Ferroptosis to Alleviate Lipopolysaccharide-induced Injury of Renal Tubular Epithelial Cells by Blocking Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling. 脂肪酸结合蛋白-4沉默通过阻断Janus激酶2/信号转导和转录激活因子3的信号传导，抑制铁凋亡以缓解脂多糖诱导的肾小管上皮细胞损伤。

Journal of physiological investigation Pub Date : 2024-01-01 Epub Date: 2024-02-28 DOI: 10.4103/EJPI.EJPI-D-23-00027

Suo Xu, Jiye Luo, Yanli Wang, Xiaobing Chen

{"title":"Fatty Acid Binding Protein-4 Silencing Inhibits Ferroptosis to Alleviate Lipopolysaccharide-induced Injury of Renal Tubular Epithelial Cells by Blocking Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling.","authors":"Suo Xu, Jiye Luo, Yanli Wang, Xiaobing Chen","doi":"10.4103/EJPI.EJPI-D-23-00027","DOIUrl":"10.4103/EJPI.EJPI-D-23-00027","url":null,"abstract":"Sepsis-induced kidney injury (SAKI) has been frequently established as a prevailing complication of sepsis which is linked to unfavorable outcomes. Fatty acid-binding protein-4 (FABP4) has been proposed as a possible target for the treatment of SAKI. In the current work, we aimed to explore the role and underlying mechanism of FABP4 in lipopolysaccharide (LPS)-induced human renal tubular epithelial cell damage. In LPS-induced human kidney 2 (HK2) cells, FABP4 expression was tested by the reverse transcription-quantitative polymerase chain reaction and Western blot. Cell counting kit-8 method assayed cell viability. Inflammatory levels were detected using the enzyme-linked immunosorbent assay. Immunofluorescence staining measured the nuclear translocation of nuclear factor kappa B p65. Thiobarbituric acid-reactive substances assay and C11 BODIPY 581/591 probe were used to estimate the level of cellular lipid peroxidation. Fe2+ content was examined by the kit. In addition, the expression of proteins related to inflammation-, ferroptosis- and Janus kinase 2 (JAK2)/signal transducer, and activator of transcription 3 (STAT3) signaling was detected by the Western blot analysis. The results revealed that FABP4 was significantly upregulated in LPS-treated HK2 cells, the knockdown of which elevated the viability, whereas alleviated the inflammation and ferroptosis in HK2 cells challenged with LPS. In addition, down-regulation of FABP4 inactivated JAK2/STAT3 signaling. JAK2/STAT3 stimulator (colivelin) and ferroptosis activator (Erastin) partially restored the effects of FABP4 interference on LPS-triggered inflammation and ferroptosis in HK2 cells. Together, FABP4 knockdown inhibited ferroptosis to alleviate LPS-induced injury of renal tubular epithelial cells through suppressing JAK2/STAT3 signaling.","PeriodicalId":519921,"journal":{"name":"Journal of physiological investigation","volume":"67 1","pages":"47-56"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central Channelopathies in Obesity. 肥胖症的中央通道病变

Journal of physiological investigation Pub Date : 2024-01-01 Epub Date: 2024-02-28 DOI: 10.4103/ejpi.EJPI-D-23-00029

Athena Hsu Li, Yi-Ying Kuo, Shi-Bing Yang, Pei-Chun Chen

引用次数: 0

The Inauguration of the Journal of Physiological Investigation. 生理学研究》杂志创刊。

Journal of physiological investigation Pub Date : 2024-01-01 Epub Date: 2024-02-28 DOI: 10.4103/ejpi.EJPI-D-24-00007

Shaw-Jenq Tsai

引用次数: 0