Asian Plant Research Journal最新文献

{"title":"Biochemical Characterization, Antimicrobial Potency, and Metallic Nanoparticle Generation from Tridax procumbens Extracts","authors":"Gbadamosi A. S., Oseni M. O., Adetukonbo B. A., Oseni O. A.","doi":"10.9734/aprj/2024/v12i4264","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i4264","url":null,"abstract":"Several studies have mainly focused primarily on seeking promising ways to solve major human diseases such as cancer, cardiovascular disease, and neuro-degenerative disease. In the quest for new antimicrobial and modifications of existing ones, interestingly, medicinal plants have emerged as the preferred and safest source of various medicines, as recommended by the World Health Organization (WHO). Plants and their bio-active compounds hold potential as antimicrobial agents to combat these diseases and are pioneers in the discovery of new medicines. Tridax procumbens is a highly promising species that produces secondary metabolites that have been reported to possess a variety of medicinal uses, including anti-anemic, anti-inflammatory, anti-diabetic, and anesthetic properties. This species has a long history of traditional use by different communities. This study aimed to investigate the medicinal properties of phytochemicals constituents and other biochemical components. The phytochemical screening, Vitamin C content antioxidants and antimicrobial properties as well as green synthesis of three metallic nanoparticles of the Tridax procumbens leaf extracts were determined using standard analytical procedures. The extracts of the leaves was found to contain medicinal important phytochemical constitutes; strong antioxidant and antimicrobial activities. The metallic nanoparticles were characterized with FTIR peaks around 4000, 3705, 2777, 1800, 1500 cm-1for AgNP, CuNP and ZnNP. Both the plant extracts and all the nanoparticles exhibited maximum inhibitory activity against the tested pathogens. Based on the results obtained in this study, this species could potentially serve as an effective, safe, and affordable source of treatment of certain ailments and drug development against major human diseases that pose threats worldwide.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"35 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141804280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-diabetic, Anti-cancer Potential and Anti HIV of Bitter Gourd (Momordica charantia) Extract: A Mini Review","authors":"Manjoo Rani","doi":"10.9734/aprj/2024/v12i4263","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i4263","url":null,"abstract":"Aims: To review the antidiabetic properties, pharmacological characteristics, and phytochemical research on Momordica Ccharantia (bitter gourd) and assess its potential as a therapeutic agent for diabetes and cancer prevention. \u0000Study Design: Review of existing literature and research studies, including pre-clinical trials and animal and in vitro studies. \u0000Methodology: Analysis of traditional uses, pharmacological data, and phytochemical research on M. Charantia. Evaluation of anti-diabetic and anti-HIV properties based on available studies. \u0000Results: \u0000 \u0000 Charantia has shown anti-diabetic and hypoglycemic effects in pre-clinical studies. \u0000Limited and unreliable clinical data due to poor research design and inadequate statistical power. \u0000Recent studies indicate potential anti-tumor, anti-diabetic, and anti-HIV properties. \u0000Plant extract components have demonstrated effectiveness in cancer prevention through immune function enhancement, induction of cell death, and inhibition of cancer-related processes. \u0000 \u0000Conclusion: M. Charantia holds promise as a therapeutic agent for diabetes management and cancer prevention. However, there is a need for better-designed clinical trials to confirm its efficacy and establish reliable clinical evidence.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141828294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoremediation of Arsenic (As), Lead (Pb), and Mercury (Hg) Contaminated Soil Using Sunflower (Helianthus annuus L.)","authors":"Lyka G. Dente, Flyndon Mark S. Dagalea, Ma. Lourdes C. Alvarez","doi":"10.9734/aprj/2024/v12i4262","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i4262","url":null,"abstract":"Phytoremediation is a cost-effective and environment-friendly approach to remediate or clean our environment using plants by accumulating contaminants. Helianthus annuus L., commonly named sunflower is known to be a hyperaccumulator of heavy metals from the soil. This study focuses on using sunflowers for the phytoremediation of heavy metal-contaminated soil, specifically Arsenic (As), Lead (Pb), ad Mercury (Hg). The researcher collected soil samples in the months of March – April 2024 at the Catarman Sanitary Landfill located at Sitio Banihit, Catarman, Northern Samar, and were analyzed for pH, Nitrogen (N), Phosphorous (P), and Potassium (K) test using a Soil Test Kit (STK) from the Bureau of Soils and Water Management – Department of Agriculture, and detected the presence of As, Pb, and Hg using qualitative analysis. The analysis showed that the soil is slightly acidic, has low nitrogen, high phosphorus, and sufficient potassium, that is an indication of heavy metal contamination, and is good for metal uptake and accumulation in plant. The researcher planted sunflower using the collected soil samples. Leaves were extracted after one (1) month and two (2) weeks, and tested for physical properties in terms of pH, density, and solubility. Results showed that sunflower leaves extract is slightly acidic with an average pH of 6.94., density of 1.00 g/ml, and shows a polar property. The plant sample was also analyzed using Colorimetric analysis for Arsenic, Flame AAS for Lead, and Cold Vapor AAS for Mercury. Findings revealed that the concentration of these three (3) heavy metals are below the detection limit. But in small concentrations, exposure to these heavy metals still poses a great threat to living organisms. Using sunflower plants of about four (4) months of more could further be tested for possible detection of considerable amount of heavy metal uptake.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"13 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141651371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Growth Stage-based Water Stress on Yield and Water Use Efficiency of Tomatoes (Solanum lycopersicon L.) in Semi-arid Regions of Tigray, Ethiopia","authors":"Ekubay Tesfay Gebreigziabher","doi":"10.9734/aprj/2024/v12i4261","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i4261","url":null,"abstract":"Water availability is a major concern in regions with limited water resources. Implementation of best irrigation water management methods can maximize crop yields and irrigation water use efficiency. An experiment was conducted in Laelay Koraro district, Tigray, Ethiopia for two consecutive years (2018 and 2019 off seasons) to investigate the effect of growth stage-based deficit irrigation on tomato yield, yield characteristics and water usage efficiency. The experiment used a randomized complete block design (RCBD) with three replications. The treatments were three irrigation levels (100%, 50%, and 25% of crop irrigation requirement) and four FAO-defined tomato growth stages (initial, developmental, mid, and late seasons). Data on agronomic parameters and irrigation water were collected and analyzed statistically. The results revealed that reducing irrigation amount by up to 75% during the development growth stage significantly decreased marketable yield by up to 66.5%. However, the highest water use efficiency (9.2kgm-3) was achieved by reducing irrigation amount by 75% at the end-growth stage of tomato. Treatments with the lowest water use efficiency (3.5kgm-3) were those receiving 75% less irrigation amount than the full requirement during the development growth stage. Irrigation deficit up to 75% of the full requirement during tomato development stages greatly affect marketable yield and water use efficiency. Therefore, the tomato crop is highly susceptible to water stress when receiving more than a 50% reduction in full irrigation requirement during its developmental growth stage.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"103 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141667194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro Anti-bacterial Activity of Methanol and Aqueous Crude Extracts of Horsfieldia iryaghedhi","authors":"Rmhkk Rajapaksha, Emn Fernando, Awmkk Bandara, N. Nelumdeniya, Arn Silva","doi":"10.9734/aprj/2024/v12i4259","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i4259","url":null,"abstract":"Aims: Over the past two decades, the rise of multidrug resistance (MDR) in bacteria has posed a significant threat to global health. The urgent need for new treatment alternatives has brought attention to the potential of plants, which harbor a wealth of unexplored phytochemicals with therapeutic properties. This study aims to evaluate the anti-bacterial efficacy of methanol and aqueous extracts from the leaves and bark of Horsfieldia iryaghedhi In vitro. \u0000Methodology: Aqueous and methanol extracts were obtained from the cold maceration method. In vitro anti-bacterial activity of methanol and aqueous leaf, bark, and combination extracts were determined against gram-negative bacteria Escherichia coli (ATCC® 25922) and gram-positive bacteria Staphylococcus aureus (ATCC® 25923). The anti-bacterial assay for different concentrations of each extract was conducted through the well-diffusion method, with Gentamycin serving as the positive control. \u0000Results: Methanol leaf and combination extracts of Horsfieldia iryaghedhi have shown a positive anti-bacterial response at their highest concentrations of 1000µg/mL and 500µg/mL against gram-positive bacteria Staphylococcus aureus while none of the extracts showed anti-bacterial activity against gram-negative E. coli at the experimented concentrations. \u0000Conclusion: The study concludes that methanol extracts of H.iryaghedhi should be further analyzed for their anti-bacterial activity, and there could be potential lead molecules that can be developed as antibiotics.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"49 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141353457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on the Nutritional, Minerals, Mineral Ratio, Anti-nutritional, Molar Ratio and Antioxidant Compositions of Four Selected Leafy Wild Vegetables in Ado-Ekiti, Ekiti State, Nigeria","authors":"Ayeni M. Janet, Oyeyemi S. Dele, Olaleye T. Favour, Adeleke B. Gladys","doi":"10.9734/aprj/2024/v12i4258","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i4258","url":null,"abstract":"The study investigated the nutritional, mineral Anti-nutritional factors, molar ratios and antioxidants compositions in the leaves of four selected wild edible vegetables (Piper guinensis, Piper umbelatum, Celosia argentia and Jatropha tajorensis) collected in Ado Ekiti, Ekiti State, Nigeria. The proximate, minerals and anti-nutrient compositions of the samples were determined using standard procedures. Also antioxidant activity of the samples were detected using standard assays such as 1, 1, diphenyl-2-2 picrylhydrazyl (DDPH) and Ferric Reducing Antioxidant Potential (FRAP).The mineral ratio, molar ratio and Mineral Safety Index were calculated from mineral results. The results of the proximate compositions for the four wild vegetables for moisture content,  ash, crude fat, crude fiber, crude protein and carbohydrate ranged from 11.16 - 12.15%, 2.76-4.03%, 4.07 -7.31%, 13.33-16.73%, 7.36 – 10.09%, and 52.09 -58.22% respectively,. The results of the mineral composition revealed high content of K, moderate content of Ca, P, and low contents of Mg, Fe, Zn, Cu and very low concentrations of Pb and Ni. Cd was absent in all the samples investigated. Zn/Cu and Fe/Cu ratios indicated low copper load. Antinutrients factors in the vegetable were low with the exception of phytate which can be reduced to non-toxic level through boiling or cooking. The calculated mole ratio for [Phy][Zn], [Ca]/[Phy], [Phy]/[Fe] and [Ca][Phy]/[Zn] conformed with the standard values. The results of the antioxidant properties showed that the wild vegetables are good natural antioxidant sources. The assessment of the vegetables showed that they are rich sources of crude protein, crude fiber and carbohydrate with high energy calories. The mineral ratios and mineral safety index suggested that the minerals could not pose any mineral load to human body. Hence, the vegetables could be explored as supplement diet for man. The vegetables could provide the needed nutrition health benefit and also help in addressing the problem of food security in Nigeria.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":" 84","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-constituents of the Dichloromethane Extracts of the Bulbs of Selected Genera in the Family Amaryllidaceae","authors":"M. E. Bassey, I. Johnny, Ogechi Queeneth Iwu, O. T. Umoh, Augustine Ogenekevwe Ochuko","doi":"10.9734/aprj/2024/v12i3255","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i3255","url":null,"abstract":"The analysis of phytoconstituents present in the dichloromethane extract of the bulb of Hippeastrum vittatum (L’Her.) Herb., Proiphys amboinensis (L.) Herb., Urceolina amazonica (Linden) Christenh. & Byng., Hymenocallis littoralis (Jacq.) Salisb., Crinum jagus (J. Thomps.) Dandy and Zephyranthes carinata Herb. by Gas Chromatography-Mass Spectometry (GC-MS) analysis was carried out using standard methods. The Results of the GC-MS analysis of Hippeastrum vittatum, Proiphys amboinensis, Urceolina amazonica, Hymenocallis littoralis, Crinum jagus and Zephyranthes carinata revealed the presence of 50, 66, 61, 57, 56 and 61 peaks indicating the presence of the number of phytochemical constituents respectively. Phytol was present in Hymennocallis littoralis (0.41%) and Urceolina amazonica (0.79%) only. N-hexadecanoic acid, Di mevalonic acid lactone, Hexadecanoic acid methyl ester and Stigmasterol were present in all. Apiol and Xanthoxylin were absent in H. littoralis and P. amboniensis respectively. Oleic acid was present in Z. carinata (2.01%), P. amboniensis (1.85%) and U. amazonica (2.03%). Sakuranin was found in H. littoralis (3.42%), C. jagus (1.38%) and U. amazonica (4.43%). Linoleic acid ethyl ester was present in P. amboniensis (1.49%), U. amazonica (0.16%), H. littoralis (11.91%)  and Z. carinata (0. 8 8%).  The major components that delimited the species were as follows; In Hymenocallis littoralis was Liriodendromin (14.03%), Linoleic acid ethyl ester (11.91%) and Ethyl oleate (9.38%); in Zephranthes carinata; Phthalic acid, 4-methoxybenzyl methyl ester (15.34%) Gatanthamine, 3-O-acetyl-1, 2, dihydro (8.87%) Stigmasterol (5.87%); in Hippeastrum vittatum; N-benzyl-2-[1-(4-methoxyl-phenyl 1) – 1 H-tetrazo (14.94%),  Benzaminde, 2-amino-N-(4-ethoxyphenyl) (12.65%) and  1-(5-methyl-2-hydroxyphenyl)-3-phenyl propane (10.49%); in Crinum jagus; 2H-1-Benzopyran-7-ol, 3,4-dihydro-3-(2-hyrdo) (19.87%),  Benzamide, 2-amino-N- (4-ethoxyphenyl) (9.92%) and Stigmasterol (4.04%); in Proiphys amboniensis; Gamma-sitosterol (16.98%), Stigmasterol (11.49%) and N-Hexadecanoic acid(5.05%); in U. amazonica; 3-Furanacetic acid, 4-hexyl-2, 5-dihydro-2, 5 (12.73%), Benzamide, 2-amino-N- (4-ethoxyphenyl) (8.18%) and Phenyl, 2-(3,4-dihydro 2-methoxyl-2H-1-benzopy) (6.40%). The presence of various bioactive compounds may be responsible for the application of these species in the treatment and management of various ailments in folklore medicine. However, in vitro and in vivo studies, isolation of individual phytoconstituents and their mechanism of action may proceed to find a novel drug or lead compound for use as medicine.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":" 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141001239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Trypsin Inhibitory Activity of Vigna unguiculata ssp. Sesquipedalis (Hawari Mae) Seeds Grown in Sri Lanka was Evaluated","authors":"YN Wickramaratne, Saadsd Peiris, Dmhsk Doranegoda, Aalt Ampemohotti, Fass Pillay, Kdkp Kumari, Arn Silva","doi":"10.9734/aprj/2024/v12i3254","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i3254","url":null,"abstract":"Aims: This study aimed to screen the entire seed sample of Vigna unguiculata ssp sesquipedalis (Hawari mae) for serine protease inhibitory activity. Additionally, the study explored the impact of various factors such as temperature, pH, metal ions, detergent, oxidizing and reducing agents on the inhibitory activity.\u0000Methodology: A batch of Hawari Mae seeds was obtained from the Plant Genetic Resources Center in Sri Lanka. A concentration gradient of aqueous seed extracts was tested for Serine Inhibitory Activity (SIA). To carry out the SIA assay, seed extracts were mixed with trypsin in phosphoric acid buffer (pH 7.6) and casein was added as the substrate. The samples were then incubated at 37°C and trichloroacetic acid was used to stop the reaction. The supernatants were checked for absorbance at 280 nm and the percentage of serine inhibitory activity (SIA%) was calculated. All experiments were conducted three times to ensure accuracy. The inhibitory activities were tested under various conditions, including different temperatures, pH levels, metal ions, detergents, and oxidizing and reducing agents.\u0000Results: The crude extract containing 10% of the substance showed the highest level of trypsin inhibitory activity at 96.03±0.005%. This particular extract was selected for further analysis. The study revealed that the enzyme inhibition activity was highest at a temperature of 37°C (95.42±0.006%) and a pH of 7.6 (95.71±0.003%). It was observed that all tested metal ions, except Cu2+, significantly reduced (P<0.05) the trypsin inhibitory activity in Hawari mae. The presence of the detergent Triton X-100 did not significantly affect (P>0.05) the trypsin inhibitory activity of Hawari mae. However, the presence of DMSO and dithiothreitol significantly (P<0.05) reduced its activity.\u0000Conclusion: Significant trypsin inhibitory activity was observed in the seed extract of Hawari mae. Further studies on the effects of various physio-chemical parameters can aid in the purification and isolation of trypsin inhibitors.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"91 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140677154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on the Chemical Constituents, Anti-inflammatory and Anti-oxidant Agents from Combretum Genus","authors":"Jude Ibeabuchi Ali, F. K. Asogwa, C. O. Ugwu, Hannah Ndidiamaka Okorie, T. Ujam, O. Obidiegwu, Festus BC Okoye","doi":"10.9734/aprj/2024/v12i3253","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i3253","url":null,"abstract":"Many diseases are treated using medicinal plants because they contain components that have therapeutic properties. The prevention and treatment of diseases linked to oxidative stress depend heavily on antioxidants. With over 20 genera and 600 species, the Combretaceae family is a vast group of plants that is distributed tropically throughout the world, with centers of diversity in Africa and Asia. In traditional medicine, certain species of Combretum are widely used as diuretics and to treat bleeding, diarrhea, infections, diabetes, malaria, and inflammation. This work is a review of Combretum species that have been assessed for their biological activity. It is imperative that more research be done on Combretum species in order to generate novel therapeutic options that could potentially enhance the well-being of individuals with a range of ailments.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"12 s3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140693005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profile and Antibacterial Effects of Zingiber Officinale Root Extract on Some Enteric Bacterial Pathogens","authors":"George Okechukwu Anyanwu, J. Dike-Ndudim, Dennis Chimezie Nwosu, Comfort Chinyere Anyanwu, Chinatu Onyeakolam Ahiara, Ogbonnaya Cornelius Chima, Festus Chidi Emengaha, Augustine Uchechukwu Obi, Isaac Onyeke Onuh, Chizaram Winners Ndubueze","doi":"10.9734/aprj/2024/v12i3252","DOIUrl":"https://doi.org/10.9734/aprj/2024/v12i3252","url":null,"abstract":"The majority of Africans today depend either totally or partially on medicinal plants for the treatment of various diseases. In some rural communities, ethno-medicine is sometimes the only form of healthcare. Therefore, this work aimed to determine the phytochemical constituents and antibacterial potentials of Z. officinale, which is one of the medicinal plants used by some people. The active ingredients of the plant were first extracted using water and ethanol as solvents. This was followed by the phytochemical analysis of the extracts. Furthermore, the antibacterial effects of aqueous and ethanolic extracts of Z. officinale on Escherichia coli, Salmonella typhi and Shigella dysenteriae isolated from faeces of gastroenteritis patients were evaluated using the agar diffusion technique (punch method). Additionally, a two-fold tube dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts. The mean values of zones of inhibition obtained were statistically analyzed using ANOVA. The least significant difference was determined according to the LSD test at P<0.05. Phytochemical analysis revealed the presence of saponin, alkaloids, flavonoids, and tannins. The antibacterial results showed that both the aqueous and ethanolic extracts have antibacterial effects against all the test organisms but at varying degrees. At the 500mg/ml concentration, the ethanolic extract of Zingiber officinale produced a zone of inhibition of 21.00b against S. dysenteriae and 20.00b against E. coli and S. typhi. On the other hand, the 500mg/ml concentration of the aqueous extract had a zone of inhibition of 20.00b against S. dysenteriae, 16.00b against E. coli and 10.00b against S. typhi. The findings from this study lend credence to the claims that Z. officinale extracts possess antibacterial potentials. Also, the higher potency in ethanolic than in aqueous extract suggests that the potency might be dose and solvent dependent. Conclusively, owing to the findings from this study, the active ingredients of Z. officinale could be harnessed and employed in the development of novel antibacterial therapies.","PeriodicalId":512286,"journal":{"name":"Asian Plant Research Journal","volume":"69 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140694558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}