Spectroscopy-An International Journal最新文献_第3页

Quantitative Evaluation of Acetaminophen in Oral Solutions by Dispersive Raman Spectroscopy for Quality Control 用色散拉曼光谱定量评价口服液中对乙酰氨基酚的质量控制

Spectroscopy-An International Journal Pub Date : 2012-06-13 DOI: 10.1155/2012/108041

V. Bório, Rubens Vinha, R. Nicolau, H. P. M. D. Oliveira, C. J. Lima, L. Silveira

{"title":"Quantitative Evaluation of Acetaminophen in Oral Solutions by Dispersive Raman Spectroscopy for Quality Control","authors":"V. Bório, Rubens Vinha, R. Nicolau, H. P. M. D. Oliveira, C. J. Lima, L. Silveira","doi":"10.1155/2012/108041","DOIUrl":"https://doi.org/10.1155/2012/108041","url":null,"abstract":"This work used dispersive Raman spectroscopy to evaluate acetaminophen in commercially available formulations as an analytical methodology for quality control in the pharmaceutical industry. Raman spectra were collected using a near-infrared dispersive Raman spectrometer (830 nm, 50 mW, 20 s exposure time) coupled to a fiber optic probe. Solutions of acetaminophen diluted in excipient (70 to 120% of the commercial concentration of 200 mg/mL) were used to develop a calibration model based on partial least squares (PLSs) applied to Raman spectra of solutions and, subsequently, obtain linearity, accuracy, precision (repeatability), and sensitivity of the method using the near-infrared spectroscopy (NIRS) as a gold standard method. This model was used to predict the acetaminophen concentration in commercial samples from different lots of acetaminophen formulations (200 mg/mL) with a PLS-prediction error of about 0.6%. Commercial medicines had PLS predicted concentrations errors below 2.5%, whereas NIRS had an error of about 3.7% compared to the label concentration. It has been demonstrated the applicability of Raman spectroscopy with fiber probe for quality control in pharmaceutical industry of commercial formulations.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"1 1","pages":"215-228"},"PeriodicalIF":0.0,"publicationDate":"2012-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79218496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Role of Vibrational Spectroscopy in Stem Cell Research 振动光谱在干细胞研究中的作用

Spectroscopy-An International Journal Pub Date : 2012-06-03 DOI: 10.1155/2012/513286

C. Aksoy, F. Severcan

{"title":"Role of Vibrational Spectroscopy in Stem Cell Research","authors":"C. Aksoy, F. Severcan","doi":"10.1155/2012/513286","DOIUrl":"https://doi.org/10.1155/2012/513286","url":null,"abstract":"Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the stem cells have some unwanted effects on the properties of stem cells, and these methods also do not provide real-time information about cellular conditions. These challenges enforce the usage of nondestructive, rapid, sensitive, high quality, label-free, cheep, and innovative chemical monitoring methods. In this context, vibrational spectroscopy provides promissing alternative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Raman and infrared spectroscopy and imaging can be used as a new complimentary spectroscopic approaches to gain new insight into stem cell reseaches for future therapeutic and regenerative medicines. In this paper, recent developments in applications of vibrational spectroscopy techniques for stem cell characterization and identification are presented.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"25 1","pages":"167-184"},"PeriodicalIF":0.0,"publicationDate":"2012-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81667242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin 氯霉素与人神经球蛋白相互作用的多光谱研究

Spectroscopy-An International Journal Pub Date : 2012-06-03 DOI: 10.1155/2012/192591

Lei Huang, Lianzhi Li, Haili Li, Chaohui Gao, Hui Cui, Xiangshi Tan

引用次数: 1

Determination of Collagen Fibers Arrangement in Bone Tissue by Using Transformations of Raman Spectra Maps 用拉曼光谱图变换测定骨组织中胶原纤维排列

Spectroscopy-An International Journal Pub Date : 2012-05-28 DOI: 10.1155/2012/261487

T. Buchwald, M. Kozielski, M. Szybowicz

引用次数: 21

Time-resolved surface-enhanced resonance Raman spectro-electrochemistry of heme proteins 血红素蛋白的时间分辨表面增强共振拉曼光谱电化学

Spectroscopy-An International Journal Pub Date : 2010-01-01 DOI: 10.3233/SPE-2010-0414

Marc Grosserueschkamp, C. Nowak, W. Knoll, R. Naumann

引用次数: 4

Magnetization‒transfer 31P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics 体内生化交换的磁化转移31P核磁共振:在肌酸激酶动力学中的应用

Spectroscopy-An International Journal Pub Date : 2002-01-01 DOI: 10.1155/2002/326454

H. Möller, D. Wiedermann

引用次数: 3

Cyclization reactions of IMM-125 and oxidation of cyclosporin A amino-acid 1 in the alpha position of the double bond lead to the loss of in vitro immunosuppressive activity IMM-125的环化反应和双键α位置环孢素A氨基酸1的氧化导致体外免疫抑制活性丧失

Spectroscopy-An International Journal Pub Date : 2000-01-01 DOI: 10.1155/2000/491283

R. Dieden, D. Latinne, C. Baldari, Nicoletta Maton, A. Aubry, R. Verbeeck, M. Zurini, G. Lhoëst

{"title":"Cyclization reactions of IMM-125 and oxidation of cyclosporin A amino-acid 1 in the alpha position of the double bond lead to the loss of in vitro immunosuppressive activity","authors":"R. Dieden, D. Latinne, C. Baldari, Nicoletta Maton, A. Aubry, R. Verbeeck, M. Zurini, G. Lhoëst","doi":"10.1155/2000/491283","DOIUrl":"https://doi.org/10.1155/2000/491283","url":null,"abstract":"Cyclosporin A (CsA) and IMM-125, a hydroxyethyl derivative of D-serine CsA, are cyclic undecapeptides of molecular weight 1201.8 and 1261.8, respectively. The main metabolites still possessing the undecapeptide structure were found to be compounds resulting from the biotransformation of amino acids 4, 9 and 1. Under the influence of the hepatic cytochrome P-450-dependent monooxygenase system, CsA and IMM-125 amino acid 1 are metabolized to a mono-hydroxylated compound (metabolite M-17) and to a dihydrodiol. A metabolite M18 was found to be the result of a non-enzymic intramolecular formation of a tetrahydrofuran derivative from metabolite M17. Since the existence of a CsA dihydrodiol was reported and since epoxides are considered as the dihydrodiol precursors, the aim of the present work was to prove that the same non-enzymic intramolecular formation of a tetrahydrofuran ring could occur by nucleophilic attack of the amino-acid 1 beta -hydroxy group at the epsilon -position of the freshly formed epoxide by reaction of IMM-125 with m-chloroperbenzoic acid and cyclosporin A with selenium oxide. The immunosuppressive activity of the compounds, as measured by the mixed lymphocyte reaction and by the luciferase activity of a Jurkat-T-cell line stably transfected with the NF-AT/luc reporter plasmid, was found negligible for IMM-125 compared to the parent drug as well as for the cyclosporin A derivative. Structures of the IMM-125 and CsA derivatives were elucidated by electrospray mass-spectrometry and NMR spectroscopy.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"1 1","pages":"215-228"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74491105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0