{"title":"Diagnostic informativeness of biopsy for chronic kidney disease in cats","authors":"O. Y. Ostrovskyi, L. Slivinska","doi":"10.32718/nvlvet11301","DOIUrl":"https://doi.org/10.32718/nvlvet11301","url":null,"abstract":"The biopsy is a diagnostic method that allows in vivo sampling of kidney tissue material for histopathological examination to identify structural changes in the organ. The study aims to study the informativeness of intravital diagnosis of stage II chronic kidney disease (according to IRIS) in cats based on the results of histological and histochemical studies of biopsies. A kidney biopsy was performed on two cats aged 6 and 8 years. Research methods: histological (hematoxylin and eosin, Heidenhain test) and histochemical (PAS reaction, Gomori (PAMS)). The diagnosis of chronic kidney disease was made based on history, clinical symptoms, and blood and urine test results. Based on the biopsy, acute extracapillary serous-desquamative glomerulonephritis was established in cats with II-stage chronic kidney disease, which was characterized by vacuolar dystrophy of renal tubule epitheliocytes, desquamation of glomerular capsule epithelium. In addition, lymphoplasmacytic infiltration of the renal stroma, thickening of the Shumlyansky-Bowman capsule with significant deposition of PAS-positive substances, the collapse of the glomerular loops, and focal atrophy of the proximal tubules of the kidney pronounced extra capillary desquamation of the glomerular epithelium, segmentation of the vascular glomerulus, and dystrophy of single tubules were noted. The detected changes in kidney biopsies of cats in the II stage of chronic kidney disease are characteristic of this pathology, which makes it possible to develop and carry out effective treatment of animals promptly and improve their quality of life.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"86 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Zaika, Z. Khomenko, S. Huralska, T. Kot, L. Bezditko
{"title":"Pathomorphological characteristics of cardiac hemangiosarcoma in a dog (a clinical case)","authors":"S. Zaika, Z. Khomenko, S. Huralska, T. Kot, L. Bezditko","doi":"10.32718/nvlvet11306","DOIUrl":"https://doi.org/10.32718/nvlvet11306","url":null,"abstract":"Hemangiosarcoma is a prevalent and lethal type of cancer in dogs. The tumour grows and spreads rapidly, often giving no warning signs to the owner until the sudden death of the dog. Hemangiosarcoma typically affects older dogs. A confirmed case study of an animal’s death shows that angiosarcoma mainly develops in dogs of a specific age and breed. The dog examined in this study was seven years old. Detecting cardiac hemangiosarcoma can be challenging, as X-ray exams may produce false-negative results, as seen in the current study. Pathological mitotic divisions are often the most recognizable sign of a heart tumour, which can be identified through an incisional biopsy. However, in our case, this was not feasible due to the patient’s rapid decline. The patient’s enlarged heart was visible on a chest X-ray, while an ultrasound revealed a pericardial effusion. Pericardiocentesis was performed, and 80 ml of hemorrhagic pericardial fluid was drained. Unfortunately, the results of the cytological examination of the fluid were negative, as confirmed by this study. Classic clinical signs of hemangiosarcoma are not present, except for abrupt and severe internal bleeding. The animal’s owner reported periodic lethargy or fatigue and shortness of breath as clinical signs. During our examination, we noted tachycardia and muted heart sounds as additional clinical indications. During the pathological examination, the changes found in the body are not specific and may differ in individual cases of death. An autopsy established the absence of visual metastatic foci and in other organs: blood filling of the spleen; blood filling and liver enlargement; speckled haemorrhages on the lung pleura, mucous membrane of the stomach and small intestine; the mucous membrane of the large intestine is covered with a large amount of mucus and multiple haemorrhages. Histopathological studies have established a cavernous hemangioma, a large cavity of various shapes, filled with blood and lined with endothelium, separated by thin connective tissue partitions. In places, the blood in the cavities coagulates, and the formed thrombotic masses yield to their organizing.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"91 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of harmful gases and bioaerosols of pig farms: a review of the existing literature","authors":"P. B. Grigorash, Y. Horiuk","doi":"10.32718/nvlvet11304","DOIUrl":"https://doi.org/10.32718/nvlvet11304","url":null,"abstract":"From the ecological and economic point of view, intensive pig farming is drawing more and more attention due to the appearance of harmful substances, which significantly increases the risks of air, water and soil pollution. In addition, the optimal criteria for the content of harmful gases, dust and microorganisms in the environment of pig farms are currently not clearly defined. In order to better understand the interaction of harmful emissions and aerosols, the article reviews data from the literature on their main components, concentrations, and interactions. The results showed that the main pollutants in pig farming are harmful gases (ammonia (NH3), carbon dioxide (CO2), methane (CH4), nitrogen oxide (N2O), hydrogen sulfide (H2S), which are released from manure. The presence of even small concentrations of harmful gases in farm air can cause respiratory and cardiovascular system disorders not only in animals, but also in humans. Along with harmful gases, it is important to control the emission of solid particles and dust from livestock premises, which can form aerosols. Microbial aerosols in pig houses contain bacteria, fungi, viruses, which mainly come from the animals themselves, manure or service personnel. The bioaerosol includes the Firmicutes and Bacteroidetes bacterial divisions and their derivatives. In addition, the content of potentially dangerous bacteria in the bioaerosol can reach up to 40 %. A major concern causes the presence of a large number of antibiotic-resistant microorganisms in the air of these farms (including MRSA). The existing strategies and methods to combat these problems are still imperfect and need to be refined. Currently, the interaction of harmful gases, dust, solid particles and microorganisms is not taken into account, which can increase the toxic effect of each other on the animal's body. Therefore, there is a need for a better understanding of these interactions in order to improve the strategy for improving the microclimate conditions by correcting the microbiota, finding and developing biological preparations that contain natural bacteria capable of neutralizing odors and disinfecting livestock premises.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"24 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Dubova, L. Kovalova, P. Kovalov, Yu. V. Kovalchuk, V. V. Karpyuk
{"title":"The shrapnel-crushed wounds in dogs: status evaluation and therapy for wound healing","authors":"O. Dubova, L. Kovalova, P. Kovalov, Yu. V. Kovalchuk, V. V. Karpyuk","doi":"10.32718/nvlvet11313","DOIUrl":"https://doi.org/10.32718/nvlvet11313","url":null,"abstract":"Determining the clinical and laboratory status of animals with crushed wounds as a result of the traumatic effect of ammunition fragments, as well as the wound healing effect of ecologically safe drugs based on natural beekeeping products is of great importance under the conditions of the animals' stay in the war zone and territories subject to rocket-bomb and artillery fire. The aim of the research is to study the clinical condition of dogs with shrapnel-crushed wounds, observing its dynamics during systemic therapy and evaluating the therapeutic effect of wound healing using the universal biological ointment Unibiol®. During the research, various physical, hematological, biochemical, hemorheological, hemodynamic, and statistical methods were employed. Six animals with shrapnel-crushed wounds on their limbs and groin were analyzed. It was established that due to the traumatic factor, injuries in dogs are characterized by extensive tissue destruction and deconstruction. The overall condition of the animals, based on clinical signs, is described as toxemic-septic. Hematological changes such as anemia, leukocytosis, thrombocytopenia, left shift in the nucleus, monocytosis, and lymphocytosis indicate a systemic inflammatory reaction and a septic state in the dogs' bodies. Biochemical studies revealed hypoalbuminemia leading to hypoproteinemia, creatinine elevation, uremia, elevated levels of indicator transaminases, suggesting multi-organ failure. A significant decrease in circulating blood volume, increased spontaneous platelet aggregation, characterizes a moderate traumatic circulatory shock, while a 5-fold increase in the circulation of D-dimer levels indicates a significant potential for disseminated intravascular coagulation syndrome. Following infusion and antibiotic therapy, wound sanitation over 5 days led to a significant restoration of the clinical-laboratory status of the injured dogs, setting the stage for Unibiol® ointment wound-healing therapy. As a result of the treatment conducted over 21 days, the wound defect healed with the formation of complete granulation tissue and an elastic scar. The clinical trials conducted allow recommending Unibiol® ointment for effective and environmentally safe healing of severe shrapnel-crushed wounds.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"92 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lakhman, T. Romanishina, M. V. Zastulka, V. Behas, O. Galatiuk
{"title":"Resistance of a mixed bacterial culture isolated from bee dysbiosis to disinfectant in laboratory conditions","authors":"A. Lakhman, T. Romanishina, M. V. Zastulka, V. Behas, O. Galatiuk","doi":"10.32718/nvlvet11308","DOIUrl":"https://doi.org/10.32718/nvlvet11308","url":null,"abstract":"Disinfection is one of the components of preventive actions to prevent disease in animals by infectious agents. Through the elimination of agents of infectious diseases in the environment, it is able to interrupt the mechanisms of the epizootic process. For the development of beekeeping and obtaining quality and products, it is necessary to carry out activities in apiaries, which include disinfection. The introduction of new disinfectants in the field is possible after preliminary laboratory tests. The aim of our research objective to investigate the resistance of a mixed bacterial culture isolated from bees with the symptoms of dysbiosis to disinfectant under laboratory conditions. Bacteriological investigations carried out in Petri dishes using meat-peptone agar and Mueller-Hinton agar. The disinfectant diluted with distilled water at concentrations of 0.25 %, 0.5 %, 1,25 % and 2,5 %. On the Mueller Hinton agar, the bacterial elimination zones recorded at 1.25 % (11,6 ± 0,45 mm) and 2.5 % (12,4 ± 0,27 mm) concentration of disinfectant at 1 day of investigation, and 7,4 ± 0,27 mm (1,25 %), 11,2 ± 0,42 mm (2.5 %) at 72 hours of the experiment, respectively. In addition, the bacterial growth inhibition effect increased with experimental time and ranged from 24.0 ± 0.35 mm (0.5 %) to 28.2 ± 0.22 mm (2.5 %) on day 3 of the study. Bacteriostatic and bactericidal effects appeared to be less pronounced on meat-peptone agar. The highest diameters of inhibition of the processes of reproduction and development of microorganisms from mixed microbial association on this medium were recorded at 1.25 % and 2.5 % concentrations of the disinfectant at the 3rd day of the investigation with diameters of the zones of inhibition of growth 15.6 ± 0.45 mm and 26.4 ± 0.27 mm, respectively. The specificity of diffusion of the investigated active substance from the discs into the agar medium thickness was also noted. This effect has a varying effect on infectious agents in in vitro experiments. Thus bacteriological laboratory experiments should be performed on several culture media in purpose of obtaining accurate results. The analysis of the obtained results indicates the demonstration of antimicrobial effect of the investigated disinfectant against mixed bacterial culture in the form of bactericidal and bacteriostatic actions. Therefore, a promising direction of research for veterinary doctors and apiary business owners is the search for effective and efficient disinfectants. Laboratory investigation of the activity of a remedy are the first step before it is approbation.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"83 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological characteristics of the kidneys of mature dogs (Canis Lupus Familiaris L., 1758)","authors":"Z. Khomenko, I. Sokulskyi, S. Zaika","doi":"10.32718/nvlvet11309","DOIUrl":"https://doi.org/10.32718/nvlvet11309","url":null,"abstract":"Each biological species of animals is unique in its structure, which is due to the uniqueness of ecological habitats and the peculiarities of adaptation of homeostasis mechanisms to the influence of environmental factors. Subtle differences in the structural and functional components of organs found in different species of animals create a clear architecture for understanding the depth of biological processes. Research of species, breed and age macro- and microanatomical structures and their morphometric indicators of organs and systems should be considered as urgent tasks of modern morphology. The new data on the morphological indicators of organs discovered at the macro- and micro-levels make a certain contribution to the age-related, breed-related, comparative, experimental and clinical morphology of mammals. Urinary organs in the body of mammals play one of the leading roles in maintaining the physiological and biochemical status of the organism and homeostasis as a whole, which largely depends directly on the morphological and functional state of these organs. Increasingly, there is a need for comprehensive scientific studies of various departments of the urinary organs. The scientific article uses material that is a fragment of the planned research work of the department of normal and pathological morphology, hygiene and expertise “Development, morphology and histochemistry of animal organs in normal and pathological conditions”, state registration number 0120U100796. The article contains the results of the morphometric characteristics of the kidneys of sexually mature dogs – Canis lupus familiaris L., 1758. During the implementation of this work, complex research methods were applied: anatomical, histological, morphometric and statistical. For histological examination, a fragment of kidneys was selected, they were fixed in 10 % neutral formalin solution, Carnois and Buena fluid. In the future, dehydration was carried out in alcohols of increasing concentration and the procedure for sealing the organ was carried out according to generally accepted methods. Using a sled microtome, histological sections with a thickness of 7–10 μm were obtained. Processing of the results of histological sections was examined under a “Micros” light microscope with a digital camera at a magnification of × 100–400. The morphological study of the structural organs of the urinary system was studied on histological sections by the method of light microscopy in accordance with the requirements of the international principles of the “European Convention for the Protection of Vertebrate Animals Used in Experiments and Other Scientific Purposes” (Strasbourg, 1986), by the decision of the First National Congress on Bioethics (M. Kyiv, 2001), Law of Ukraine No. 692 “On the Protection of Animals from Cruel Treatment” (3447-IV) dated February 21, 2006. According to the histological structure, the kidneys of dogs are represented by renal corpuscles and renal tubules, which ","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Dunaievska, I. Sokulskiy, L. Horalskyi, M. L. Radzikhovskyi, B. Gutyj
{"title":"Peculiarities of morphological indicators of quail spleen (Coturnix japonica Temminck et Schlegel, 1849) when using protein-vitamin mineral supplements","authors":"O. Dunaievska, I. Sokulskiy, L. Horalskyi, M. L. Radzikhovskyi, B. Gutyj","doi":"10.32718/nvlvet11303","DOIUrl":"https://doi.org/10.32718/nvlvet11303","url":null,"abstract":"In most countries of the world, poultry farming is one of the main branches of agriculture, which provides the population with high-quality food products. To date, the positive dynamics of the growth of such a branch of industrial poultry farming as quail breeding has been noted. Quails have a complex of excellent economic and productive advantages compared to other agricultural birds – high body temperature, intensive metabolism in the body, small size, precocity, high egg productivity. The question of increasing the productivity of quail breeding, as a branch of precocious animal husbandry, has always occupied the attention of biologists, morphologists, physiologists, geneticists and technologists. This topical problem has various scientific aspects: morphological, histochemical, physiological, genetic, etc. Special attention in the conducted research is required to study the impact of additives on the body of quails, the absence of negative effects and the production of safe products for humans. We chose the spleen as a biomarker, because the spleen is an important peripheral organ of immune protection, sensitive to the action of various factors, whose morphofunctional indicators determine the magnitude and nature of the impact. The purpose of the research was to determine the effect of modern protein-vitamin mineral supplements, which are advertised and offered by producers in the poultry industry to stimulate the growth and development of the bird, on the quail body. In the studies, the features of the microscopic structure and morphometry of the spleen of control and experimental animals were used as sensitive characteristics of the action of additives. The proposed methods are aimed at researching the impact of protein-vitamin mineral supplements on the relevant industry. So, for 21 days, mineral supplements “Multilife”, “Missy” and “Standard Agro” were added to the main ration of quails. Using macrometric methods, the body weight of quails, the weight of the spleen (absolute and relative) were determined. For histological studies, pieces of the spleen were fixed in a 10 % formalin solution, and after embedding in paraffin, histological sections were prepared from it, which after deparaffinization were stained with hematoxylin and eosin, according to the methods of Brachet and Van Gieson. For electron microscopic examination, the spleen was immediately fixed with a 2.5 % solution of glutaraldehyde in a phosphate buffer with additional fixation in a 1 % solution of osmium tetroxide according to Caulfield. For the first time, the ultrastructural organization of the spleen of Japanese quail was investigated after feeding protein-vitamin mineral supplements, which may indicate the absence of their negative impact on the structural and functional state of the spleen. Important morphological structural indicators of the morphofunctional state of the spleen were determined: the relative area of the white pulp, red pulp, lymphoid nodules, periarte","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"36 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140435403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shevchenko, O. V. Zhurenko, D. Feshchenko, O. Dubova, O. Zghozinska
{"title":"Detailing the chemical composition of roasted soybeans and the effect for its use in the diet of dairy cows","authors":"A. Shevchenko, O. V. Zhurenko, D. Feshchenko, O. Dubova, O. Zghozinska","doi":"10.32718/nvlvet11312","DOIUrl":"https://doi.org/10.32718/nvlvet11312","url":null,"abstract":"Roasted soybeans are a complete source of indigestible protein in the rumen, have high sanitary and hygienic indicators, are devoid of non-nutritional substances of ordinary raw soybeans. The SoyPass and RaPass technology provides all the advantages of roasting soybeans and creates prerequisites for prolonged storage of beans for up to two years without prior drying. The introduction of full-fat flattened soybeans roasted by the Roast-A-Matic roaster into the diet of dairy cows leads to an increase in their milk productivity. Certified results of chemical research show that after frying, the percentage of crude protein significantly increased (by 2.92 points, P < 0.05) and the content of total digestible nutrients (by 8.1 %, P < 0.05), urease activity decreased to 0.06 ± 0.007 units of pH. The biological value of soy protein after frying corresponds to the indicators of the reference protein. Two groups of cows were involved in the study: № 1 – high-yielding cows with a daily milk yield of 30.69–35.96 L (n = 307) and № 2 – medium- and low-yielding cows with a yield of 19.05–28.88 L (n = 377). Group № 1 was given 300 g of roasted soybeans (111 g of protein) daily in their diet instead of 250 g of a high-protein supplement (200 g of protein); group No. 2 – gradually replaced 230 g of the supplement (184 g of protein) and 1500 g of 51 % meal (765 g of protein) with 1700 g of roasted soybeans (629 g of protein). In both groups, the 65-day observation period should be divided into 2 periods: 1) a 30-day period of intensive growth with a rapid increase in milk yield (up to +24.2–25.0 %); 2) the period of stabilization (from the 31st to the 65th day), characterized by some fluctuations in the % increase in milk, but in general by reliable maximum values (up to +25.0–36.7 %). The maximum daily volume in group № 1 reached 41.83 ± 1.04 and 41.83 ± 0.3 L (Р < 0.01) on the 32nd and 38th days, respectively. At the same time, the range of the indicators of the individual yield in the group ranged from 10.7 to 11.7 L. Similar indicators for group № 2 were: 32.56 ± 2.74 L (Р < 0.025) on the 58th day and 10.4–23.8 L. So, in the first 30 days of feeding full-fat fried soybeans, there is a rapid but unstable (with fluctuating peaks) increase in milk productivity of cows. During the 2nd month of soy consumption, the maximum milk yield is reached, when the increase in added milk is not rapid, but stable. Thus, it is advisable to introduce 1.5–2.0 kg/day of roasted soybeans (equivalent to 0.3–0.4 kg of fat/day) into the diet of dairy cows.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"5 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Buchkovska, O. Chechet, V. Kovalenko, O. Vishchur, V. Baranov, S. Zakharin, M. Asanova, M. V. Kuchynskyi, B. Gutyj
{"title":"Research on the properties of the biocidal drug “Iodosan”","authors":"H. Buchkovska, O. Chechet, V. Kovalenko, O. Vishchur, V. Baranov, S. Zakharin, M. Asanova, M. V. Kuchynskyi, B. Gutyj","doi":"10.32718/nvlvet11307","DOIUrl":"https://doi.org/10.32718/nvlvet11307","url":null,"abstract":"The use of various active substances in the composition of biocides can affect specific chemical properties. Their compatibility, solubility, stability, and corrosion activity are investigated against the background of their ability to affect pathogenic microorganisms. Due to the obtained results, the manufacturer can offer their use in specific conditions (aerosol or wet use, the possibility of use on metal structures, etc.). This publication presents the results of the study of the physicochemical properties of the biocidal agent “Iodosan”, developed by the employees of State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, based on iodine and lactic acid, dark yellow, with a moderate specific smell of iodine. The starting solution of the product is straightforward to slightly yellowish liquid with a mild iodine smell; after diluting it in tap water, the color of the water remains transparent. The agent is mixed with water to prepare a working solution, “Iodosan”, for wet and aerosol disinfection of objects. The biocidal agent “Iodosan” solution in concentrations of 0.2‒0.5 % has low corrosion activity against the treated test objects. According to research results, the most insufficient corrosion activity of the drug “Iodosan” for aluminum is observed at its concentration of 0.1‒0.5 %, steel, and galvanized steel ‒ 0.1‒0.2 %. Studies have shown that the disinfectant “Iodosan”has a surface tension of 62.03 mN/m at 20 °C. This proved that the drug has a good wetting ability, which affects the disinfecting properties. In working concentrations, the drug has a pH of 5.8. The research results confirmed the effectiveness of the biocidal preparation “Iodosan” for treating premises and equipment surfaces in optimal bactericidal concentrations, and it is recommended for use in production.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Babkina, O. Haidei, I. Oleksiienko, S. Shuliak, O. Chechet, B. Gutyj, O. Krushelnytska
{"title":"Analysis of the results of study allergens in food in 2021","authors":"M. Babkina, O. Haidei, I. Oleksiienko, S. Shuliak, O. Chechet, B. Gutyj, O. Krushelnytska","doi":"10.32718/nvlvet11302","DOIUrl":"https://doi.org/10.32718/nvlvet11302","url":null,"abstract":"One of the biggest problems in the world is allergy, the clinical manifestations of which can be of varying degrees of severity – from dermatitis to Quincke's edema and anaphylactic shock. More than 250 million people suffer from food allergies. It has been scientifically proven that more than 70 food products can cause allergies. The main allergens are products of plant and animal origin, such as nuts, cereals, fish and seafood, eggs, etc. Celiac disease is one of the manifestations of allergy or individual intolerance to cereal plants. This genetic, autoimmune disease manifests itself due to the consumption of gluten-containing products occurs with a frequency of 1:3000. According to the current Ukrainian legislation, product manufacturers are obliged to indicate on the packaging of products information about the presence of food allergens, but very often they do not do this, misleading buyers and putting people who have such a problem at risk. Considering the above, there is a need to conduct state control of food products for the presence of allergens to control manufacturers regarding the labeling of allergens. The work aims to analyze the results of real-time state control of allergens in food products by PCR. The analysis of the studies of allergens in food products showed that 25.4 % contained allergens not indicated on the product packaging. This means insufficient control by manufacturers and severely violates current legislation. According to the results of the State Allergen Control Program, undeclared allergens were found in 25.4 % of food products from the total number of tested samples: in 9.3 % of sweets, 6.9 % of bakery products, 2.3 % of beverages, 6.9 % of precise products. Considering the urgency of the problem, there is a need to develop and implement a state program for monitoring allergens in food products in order to control the requirements of the current legislation.","PeriodicalId":510038,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"69 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}