Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-05-01DOI: 10.1111/azo.12554
Anurupa Goswami, Dipali Devi
{"title":"Structural Characterisation of the Silk Gland of the Endemic Silkworm, Antheraea assamensis Helfer","authors":"Anurupa Goswami, Dipali Devi","doi":"10.1111/azo.12554","DOIUrl":"https://doi.org/10.1111/azo.12554","url":null,"abstract":"<div>\u0000 \u0000 <p>Silkworms spin the highly prized and versatile silk among all the silk-secreting insects. The silk gland of these silkworms is the most efficient protein synthesizing system yielding a good variety of silk cocoons. <i>Anthereaea assamensis</i> Helfer is an endemic and unique non-mulberry silkworm species belonging to the Brahmaputra valley of Assam and adjoining hilly areas. Herein this study, the silk gland was dissected out and divided into three distinctive parts: posterior (PSG), middle (MSG) and anterior (ASG) silk gland. Various microscopic techniques were adopted to understand the histological and ultrastructural components of the silk gland along with the flow of liquid silk in it. The fluorescence microscopic examination of the three silk gland parts showed nuclear ramification with an increasing pattern during the 4th and 5th instars. Light microscopic study of the PSG and MSG showed a tight secretory nuclear structure with a distinct epithelium and an unorganised, thin epithelial structure with large secretory vesicles, respectively. The ultrastructural study of the 5th instar matured larval PSG displayed a uniform and thick layer of basement membrane. MSG showed similar characteristics with the occurrence of sparsely distributed infoldings. This first comprehensive organisational study of the silk gland parts of <i>A. assamensis</i> Helfer revealed the structural features responsible for silk synthesis, storage and its secretion.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"257-269"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148102817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Integrative Insight Into the Taxonomic Validity and Morphological Variation in the Malay Puntius Complex (Cypriniformes: Cyprinidae)","authors":"Faezeh Yazdani Moghaddam, Ehsan Damadi, Nadiatul Hafiza Hassan, Amir Jahani Gharehsou","doi":"10.1111/azo.12556","DOIUrl":"https://doi.org/10.1111/azo.12556","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Puntius sensu lato</i> has many species but is seen as an artificial grouping for unrelated cyprinids. The correct taxonomic identification and taxonomic status of the <i>Puntius</i> complex have been challenged due to their minor morphological differences. This study investigates the morphological variation and taxonomic validity of 10 species within the Malay <i>Puntius</i> complex using an integrative approach. In the traditional analysis, a one-way analysis of variance (ANOVA) revealed that 28 out of 30 traits (20 morphometric and 10 meristic characters) were significant. Discriminant function analysis (DFA) indicated significant morphological variation among morphotypes, particularly in meristic traits. This analysis correctly classified 82.8% of the originally grouped and 74.5% of the cross-validated groups based on meristic characters. Truss analysis indicated that 14 out of 22 characters were significant (<i>p</i> < 0.05). The geometric morphometric analysis was largely consistent with the morphometric and truss analyses, confirming the validity of the distinct species. Molecular phylogenetic analyses of mitochondrial COI gene sequences confirmed the validity of all species. However, consistent with morphological data, they also supported the non-monophyly of the genus <i>Barbonymus</i>.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"270-282"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148119180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphology of the Female Reproductive System of the Water Scorpion Nepa cinerea Linnaeus, 1758 (Nepomorpha: Heteroptera)","authors":"Nurcan Özyurt Koçakoğlu, Hakan Özdamar, Hicret Arslan, Selami Candan","doi":"10.1111/azo.70003","DOIUrl":"https://doi.org/10.1111/azo.70003","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Nepa cinerea</i> is an aquatic predatory insect that plays a key role in the biological control of mosquito populations. This study investigates the morphological structure of the adult female reproductive system using stereomicroscopy, light microscopy and scanning electron microscopy. The reproductive system comprises two ovaries, lateral oviducts, a common oviduct, a spermatheca, and a genital chamber. Each ovary contains five telotrophic meroistic ovarioles, which are divided into four regions: a terminal filament, a germarium with trophocytes and early-stage oocytes, a vitellarium containing previtellogenic, vitellogenic, and choriogenic oocytes, and a long pedicel. Choriogenic oocytes display distinct endochorion and exochorion layers. Each pedicel contains 2–3 pale yellow, oval eggs with brown respiratory horns. Pedicels connect to the common oviduct via the lateral oviducts. The spermatheca, composed of a distal reservoir and a proximal duct, also opens into the common oviduct. The reservoir is cuticle-lined and surrounded by longitudinal muscles and glandular cells. Features such as ovariole number and the presence of respiratory horns differ among aquatic heteropterans. These findings offer valuable insights into the comparative morphology of female reproductive systems in aquatic Heteroptera.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"377-383"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148126070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-07-15DOI: 10.1111/azo.70001
Karmine Pasinatto, Alexandre O. Almeida, Mariana Terossi
{"title":"First Zoeal Stage of the Snapping Shrimp Alpheus Fabricius, 1798 (Caridea: Alpheidae): New Description of Alpheus bouvieri A. Milne-Edwards, 1878 and Literature Review","authors":"Karmine Pasinatto, Alexandre O. Almeida, Mariana Terossi","doi":"10.1111/azo.70001","DOIUrl":"https://doi.org/10.1111/azo.70001","url":null,"abstract":"<p>Based on adult morphological characters, the more than 300 species of <i>Alpheus</i> were divided into seven informal species groups, but some groups are not supported by molecular data. The aim here was to describe the zoea I of <i>Alpheus bouvieri</i>, and to do a review of the larval descriptions available in the literature, analysing whether the morphological groups proposed for adults are supported by larval data. The new larvae were obtained from females sampled in Pernambuco and Rio Grande do Norte, Brazil. <i>Alpheus bouvieri</i> zoea I shares a combination of characters with most larvae in the genus, but with some diagnostic characteristics: endopod of the maxillule and maxilla with 3 and 4 setae, respectively, the base of the first maxilliped with 9 setae, endopod of the second and third maxilliped with 5 and 6 segments, respectively. In our review, 23 species were analysed from five groups: <i>edwardsi</i> (thirteen), <i>sulcatus</i> (four), <i>brevirostris</i> (three), <i>macrocheles</i> (two), and <i>obesomanus</i> (one) group; the zoea I of species from <i>diadema</i> and <i>crinitus</i> groups are unknown. Based on this review, many issues regarding mistakes in the zoea I description of <i>Alpheus</i> are pointed out, and the hypothesis that the morphological groups proposed for adults are supported by larval data has been rejected.</p>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"361-376"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/azo.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148110241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphology of the Female Reproductive System of the Water Scorpion Nepa cinerea Linnaeus, 1758 (Nepomorpha: Heteroptera)","authors":"Nurcan Özyurt Koçakoğlu, Hakan Özdamar, Hicret Arslan, Selami Candan","doi":"10.1111/azo.70003","DOIUrl":"https://doi.org/10.1111/azo.70003","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Nepa cinerea</i> is an aquatic predatory insect that plays a key role in the biological control of mosquito populations. This study investigates the morphological structure of the adult female reproductive system using stereomicroscopy, light microscopy and scanning electron microscopy. The reproductive system comprises two ovaries, lateral oviducts, a common oviduct, a spermatheca, and a genital chamber. Each ovary contains five telotrophic meroistic ovarioles, which are divided into four regions: a terminal filament, a germarium with trophocytes and early-stage oocytes, a vitellarium containing previtellogenic, vitellogenic, and choriogenic oocytes, and a long pedicel. Choriogenic oocytes display distinct endochorion and exochorion layers. Each pedicel contains 2–3 pale yellow, oval eggs with brown respiratory horns. Pedicels connect to the common oviduct via the lateral oviducts. The spermatheca, composed of a distal reservoir and a proximal duct, also opens into the common oviduct. The reservoir is cuticle-lined and surrounded by longitudinal muscles and glandular cells. Features such as ovariole number and the presence of respiratory horns differ among aquatic heteropterans. These findings offer valuable insights into the comparative morphology of female reproductive systems in aquatic Heteroptera.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"377-383"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148126071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-07-02DOI: 10.1111/azo.70000
Christopher G. Vopal, Zachary J. Loughman
{"title":"Life History of Tug Valley Crayfish Cambarus hatfieldi Loughman, Fagundo, Lau, Welsh & Thoma 2013 (Decapoda: Astacoidea: Cambaridae) in Southwestern West Virginia, USA","authors":"Christopher G. Vopal, Zachary J. Loughman","doi":"10.1111/azo.70000","DOIUrl":"https://doi.org/10.1111/azo.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>Life history data for <i>Cambarus hatfieldi</i> Loughman, Fagundo, Lau, Welsh, & Thoma, 2013 were collected from March through December 2018 from Dry Fork at War, West Virginia, USA. <i>Cambarus hatfieldi</i> is syntopic with <i>Cambarus callainus</i> Thoma, Loughman & Fetzner, 2014 through part of its range, with the latter having been listed as threatened by United States Fish & Wildlife Service under the Endangered Species Act in 2016. No ovigerous females with pleopodal eggs were collected; however, mean ovarian egg diameter peaked in July, which correlated with ovigerous females from the West Liberty Crayfish Collection which were collected in August 2009 at nearby Barrenshe Creek. Mature male total carapace length (TCL) ranged from 29.3 to 52.6 mm and mature female TCL ranged from 29.7 to 48.1 mm. Morphometric analysis provided evidence of form change in female <i>C. hatfieldi</i>. Relative abundance of form I males to form II males was greater in March, April, May, October and December; declining below form II going into the summer. Six size classes, or cohorts, were determined by analysis of peaks on monthly histograms of TCL. <i>Cambarus hatfieldi</i> young of the year (YOY) appear to enter the population around October, with delayed growth until Spring.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"348-360"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148129210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-07-02DOI: 10.1111/azo.70000
Christopher G. Vopal, Zachary J. Loughman
{"title":"Life History of Tug Valley Crayfish Cambarus hatfieldi Loughman, Fagundo, Lau, Welsh & Thoma 2013 (Decapoda: Astacoidea: Cambaridae) in Southwestern West Virginia, USA","authors":"Christopher G. Vopal, Zachary J. Loughman","doi":"10.1111/azo.70000","DOIUrl":"https://doi.org/10.1111/azo.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>Life history data for <i>Cambarus hatfieldi</i> Loughman, Fagundo, Lau, Welsh, & Thoma, 2013 were collected from March through December 2018 from Dry Fork at War, West Virginia, USA. <i>Cambarus hatfieldi</i> is syntopic with <i>Cambarus callainus</i> Thoma, Loughman & Fetzner, 2014 through part of its range, with the latter having been listed as threatened by United States Fish & Wildlife Service under the Endangered Species Act in 2016. No ovigerous females with pleopodal eggs were collected; however, mean ovarian egg diameter peaked in July, which correlated with ovigerous females from the West Liberty Crayfish Collection which were collected in August 2009 at nearby Barrenshe Creek. Mature male total carapace length (TCL) ranged from 29.3 to 52.6 mm and mature female TCL ranged from 29.7 to 48.1 mm. Morphometric analysis provided evidence of form change in female <i>C. hatfieldi</i>. Relative abundance of form I males to form II males was greater in March, April, May, October and December; declining below form II going into the summer. Six size classes, or cohorts, were determined by analysis of peaks on monthly histograms of TCL. <i>Cambarus hatfieldi</i> young of the year (YOY) appear to enter the population around October, with delayed growth until Spring.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"348-360"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148129211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-06-06DOI: 10.1111/azo.12559
Qian-Ru Shen, Jing-Bang Liu, Pan-Pan Zhang, Jose Vicente Pérez Santa-Rita, Gui-Lin Hu
{"title":"Comparative Egg Morphology of Four Species of Erebidae (Lepidoptera) From China","authors":"Qian-Ru Shen, Jing-Bang Liu, Pan-Pan Zhang, Jose Vicente Pérez Santa-Rita, Gui-Lin Hu","doi":"10.1111/azo.12559","DOIUrl":"https://doi.org/10.1111/azo.12559","url":null,"abstract":"<div>\u0000 \u0000 <p>The chorionic sculpture of the eggs of <i>Euproctis pulverea</i> (Leech, 1888), <i>Lymantria dissoluta</i> Swinhoe, 1903, <i>Arctornis nivea</i> Chao, 1987, and <i>Spilarctia subcarnea</i> (Walker, 1855), was examined and described in detail by utilising light and scanning electron microscopy. <i>Euproctis pulverea</i> could be distinguished from <i>L. dissoluta</i> by the egg densely covered with hairs and the sculpture only displayed in the micropylar area. <i>Arctornis nivea</i> is unique in that the apex of the egg is dish-shaped and the bottom is smoothed, and the micropyles opening five star-shaped. <i>Spilarctia subcarnea</i> is characterised by four or five star-shaped micropyles of the egg chorion, concaved primary cells with intertwined fibres, and numerous aeropyles expressed on the ridges. We briefly discussed the significance of egg chorionic morphology in taxonomic and systematic studies in Erebidae.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"328-336"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148131380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-05-01DOI: 10.1111/azo.12554
Anurupa Goswami, Dipali Devi
{"title":"Structural Characterisation of the Silk Gland of the Endemic Silkworm, Antheraea assamensis Helfer","authors":"Anurupa Goswami, Dipali Devi","doi":"10.1111/azo.12554","DOIUrl":"https://doi.org/10.1111/azo.12554","url":null,"abstract":"<div>\u0000 \u0000 <p>Silkworms spin the highly prized and versatile silk among all the silk-secreting insects. The silk gland of these silkworms is the most efficient protein synthesizing system yielding a good variety of silk cocoons. <i>Anthereaea assamensis</i> Helfer is an endemic and unique non-mulberry silkworm species belonging to the Brahmaputra valley of Assam and adjoining hilly areas. Herein this study, the silk gland was dissected out and divided into three distinctive parts: posterior (PSG), middle (MSG) and anterior (ASG) silk gland. Various microscopic techniques were adopted to understand the histological and ultrastructural components of the silk gland along with the flow of liquid silk in it. The fluorescence microscopic examination of the three silk gland parts showed nuclear ramification with an increasing pattern during the 4th and 5th instars. Light microscopic study of the PSG and MSG showed a tight secretory nuclear structure with a distinct epithelium and an unorganised, thin epithelial structure with large secretory vesicles, respectively. The ultrastructural study of the 5th instar matured larval PSG displayed a uniform and thick layer of basement membrane. MSG showed similar characteristics with the occurrence of sparsely distributed infoldings. This first comprehensive organisational study of the silk gland parts of <i>A. assamensis</i> Helfer revealed the structural features responsible for silk synthesis, storage and its secretion.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"257-269"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148102616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta ZoologicaPub Date : 2026-06-01Epub Date: 2025-06-10DOI: 10.1111/azo.12560
Nurcan Özyurt Koçakoğlu, Üzeyir Çağlar, Hicret Arslan, Selami Candan
{"title":"Histological and Electron Microscopy Observations on the Alimentary Canal and Malpighian Tubules of the Strawberry Pest Coraebus elatus (Fabricius, 1787) (Coleoptera, Buprestidae)","authors":"Nurcan Özyurt Koçakoğlu, Üzeyir Çağlar, Hicret Arslan, Selami Candan","doi":"10.1111/azo.12560","DOIUrl":"https://doi.org/10.1111/azo.12560","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Coraebus elatus</i> (Fabricius, 1787) (Coleoptera, Buprestidae) is known as a strawberry pest, making it an essential target for pest control strategies. Despite its impact, no studies have examined the anatomical and histological structures of the alimentary canal and Malpighian tubules of <i>C. elatus</i>. Therefore, this study aimed to describe these structures in detail, which could provide insights into potential control methods. Ten individuals were collected and histologically processed, with observations made using stereo, light and electron microscopies. The alimentary canal is divided into three sections: the foregut, midgut and hindgut. The foregut comprises the pharynx, oesophagus, crop and proventriculus. A gastric caeca pair lies at the proximal anterior end of the midgut. The midgut is the longest portion. The Malpighian tubules, whose proximal ends open into the midgut and hindgut, have distal ends that connect to the colon wall. The hindgut is further subdivided into the ileum, colon and rectum. The rectum, the final segment of the alimentary canal, has a thick muscular wall and a more pronounced intima layer than the other hindgut regions. Cross sectional SEM images show six rectal pads where the final absorption of nutrients occurs. These findings may lead to new strategies for controlling this agricultural pest. Understanding these structures is crucial for further research.</p>\u0000 </div>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"107 3","pages":"337-347"},"PeriodicalIF":1.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148105454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}