In Vitro and In Vivo Effect of Amitraz on Honey Bee Drone Sperm Quality

IF 1.3 4区 生物学 Q4 ANATOMY & MORPHOLOGY
Acta Zoologica Pub Date : 2025-05-01 DOI:10.1111/azo.12555
Janice Faith Murray, Gerhard van der Horst, Mike Allsopp, Retha Christina Magrietha Kotzé
{"title":"In Vitro and In Vivo Effect of Amitraz on Honey Bee Drone Sperm Quality","authors":"Janice Faith Murray,&nbsp;Gerhard van der Horst,&nbsp;Mike Allsopp,&nbsp;Retha Christina Magrietha Kotzé","doi":"10.1111/azo.12555","DOIUrl":null,"url":null,"abstract":"<p>Honey bee hive infestation by the ectoparasitic mite, <i>Varroa destructor</i>, has been a major cause of declining populations. Treating such infestations is important for sustainability. Knowledge about the sub-lethal effects of commonly used acaricides on drone reproductive health, particularly sperm quality, is limited. The effect of in vitro and in vivo acaricide (amitraz) exposure was evaluated using Cape honey bee (<i>Apis mellifera capensis</i>) drone body size and sperm quality. For the in vitro study, semen samples were exposed to three concentrations of amitraz (125, 250, 500 μM). The in vivo study entailed in-hive Apivar treatment as per commercial recommendations. Adult drones were harvested following three weeks of treatment. Drone body weight (determined in the in vivo study only) was significantly higher in amitraz-exposed drones. No correlations were found between drone weight, sperm volume or concentration. In both studies, amitraz exposure had statistically insignificant effects on sperm motility and structure, while vitality significantly decreased in the 500 μM amitraz-exposed in vitro group. Sperm structure remained unaffected in both studies. This study highlighted the absence of sub-lethal effects of high doses of amitraz on sperm functionality. When used as advised, amitraz exposure does not have a detrimental effect on drone sperm quality.</p>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"106 4","pages":"493-502"},"PeriodicalIF":1.3000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/azo.12555","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Zoologica","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/azo.12555","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Honey bee hive infestation by the ectoparasitic mite, Varroa destructor, has been a major cause of declining populations. Treating such infestations is important for sustainability. Knowledge about the sub-lethal effects of commonly used acaricides on drone reproductive health, particularly sperm quality, is limited. The effect of in vitro and in vivo acaricide (amitraz) exposure was evaluated using Cape honey bee (Apis mellifera capensis) drone body size and sperm quality. For the in vitro study, semen samples were exposed to three concentrations of amitraz (125, 250, 500 μM). The in vivo study entailed in-hive Apivar treatment as per commercial recommendations. Adult drones were harvested following three weeks of treatment. Drone body weight (determined in the in vivo study only) was significantly higher in amitraz-exposed drones. No correlations were found between drone weight, sperm volume or concentration. In both studies, amitraz exposure had statistically insignificant effects on sperm motility and structure, while vitality significantly decreased in the 500 μM amitraz-exposed in vitro group. Sperm structure remained unaffected in both studies. This study highlighted the absence of sub-lethal effects of high doses of amitraz on sperm functionality. When used as advised, amitraz exposure does not have a detrimental effect on drone sperm quality.

Abstract Image

咪唑嗪对蜜蜂雄蜂精子质量的体内外影响
蜜蜂种群数量下降的一个主要原因是蜜蜂被寄生性螨(瓦螨破坏者)侵入蜂巢。处理这种虫害对可持续发展很重要。关于常用杀螨剂对无人机生殖健康,特别是精子质量的亚致死效应的了解有限。利用角蜂(Apis mellifera capensis)的雄蜂体型和精子质量来评价体内和体外杀螨剂(amitraz)暴露的影响。在体外研究中,精液样本暴露于三种浓度(125、250、500 μM)的阿米特拉兹中。根据商业建议,体内研究需要在蜂巢内进行Apivar治疗。经过三周的处理,收获了成虫。暴露于氨咪唑的无人机体重(仅在体内研究中确定)明显更高。没有发现雄蜂体重、精子体积或浓度之间的相关性。在两项研究中,阿米特拉兹暴露对精子活力和结构的影响均无统计学意义,而500 μM阿米特拉兹暴露组精子活力显著降低。在两项研究中,精子结构未受影响。这项研究强调了高剂量阿米特拉茨对精子功能没有亚致死效应。如果按照建议使用,阿米特拉兹不会对雄蜂精子质量产生有害影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Acta Zoologica
Acta Zoologica 生物-动物学
CiteScore
2.90
自引率
8.30%
发文量
35
审稿时长
>12 weeks
期刊介绍: Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信