{"title":"Single molecular functional assay of ferritin arrays","authors":"Mariko Yamaki","doi":"10.1016/S0065-227X(97)89640-2","DOIUrl":"10.1016/S0065-227X(97)89640-2","url":null,"abstract":"<div><p><em>In situ</em> functional assay of each ferritin molecule in single-layer 2D arrays for horse spleen apoferritin and recombinant horse L- and human H-apoferritins was conducted by observing the iron-cores formed in the arrays by TEM. The study of the time-course, pH-dependence, and temperature-dependence of the function confirmed the iron-core formation to be due to the native function of apoferritins in array. Dark-field TEM imaging revealed that there was crystallinity in the cores in the array of recombinant human H-apoferritin. This iron-core formation was perfectly preserved in the array even after 3 months of storage at room temperature and low humidity. Moreover, about 50% of the function was found to remain in the array after it was exposed to 150 ° C <em>in vacuum</em> for 1 hr.</p></div>","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"34 ","pages":"Pages 191-203"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0065-227X(97)89640-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20149824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Notice to authors","authors":"","doi":"10.1016/S0065-227X(97)89627-X","DOIUrl":"https://doi.org/10.1016/S0065-227X(97)89627-X","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"34 ","pages":"Page ix"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0065-227X(97)89627-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134651253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ribosome recycling by ribosome recycling factor (RRF) — An important but overlooked step of protein biosynthesis","authors":"Laszlo Janosi, Hiroto Hara, Shijie Zhang, Akira Kaji","doi":"10.1016/0065-227X(96)84743-5","DOIUrl":"10.1016/0065-227X(96)84743-5","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"32 ","pages":"Pages 121-201"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0065-227X(96)84743-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19753223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Origin and early evolution of eukaryotes inferred from the amino acid sequences of translation elongation factors 1α/Tu and 2/G","authors":"Tetsuo Hashimoto, Masami Hasegawa","doi":"10.1016/0065-227X(96)84742-3","DOIUrl":"10.1016/0065-227X(96)84742-3","url":null,"abstract":"<div><p>Phylogenetic placements of archaebacteria and protozoa are important in understanding the origin and early evolution of eukaryotes. These problems have been analyzed mainly by comparisons of small subunit ribosomal RNA (SrRNA) sequences. However, the SrRNA phylogeny may sometimes be unreliable, especially when base compositions are biased among species. Because it is difficult to take full account of the bias in inferring the SrRNA tree, alternative examinations using protein sequence data have been very much desired. We analyzed the phylogenetic relationship among eukaryotes, archaebacteria, and eubacteria by the ML method of protein phylogeny using amino acid sequence data of EF-1α/Tu and 2/G. The unrooted tree analyses of both the EF-1α/Tu and 2/G consistently demonstrated that the ‘eocyte’ tree, in which archaebacteria are not monophyletic but eocytes are closer to eukaryotes than to other archaebacteria, is very likely. Further analysis using a composite tree of EF-1α/Tu and 2/G suggested that archaebacteria are closer to eukaryotes than to eubacteria but are not monophyletic. These results clearly support the hypothesis that eukaryotes have evolved from the eocyte-like organism. We also analyzed a protozoan phylogeny including mitochondrion-lacking species by the ML method using EF-1α and EF-2 data sets, and demonstrated (a) that two mitochondrion-lacking species, <em>G. plecoglossi</em> (Microsporidians) and <em>G. lamblia</em> (Diplomonads) probably represent the first and the second earliest offshoots of eukaryotes, respectively; (b) that <em>Trypanosoma</em> is not likely to have diverged next to <em>Giardia</em> as suggested by the SrRNA tree, but shows high affinity with higher eukaryotes; and (c) that protein phylogeny would give a robust estimation because amino acid compositions of conservative proteins do not differ significantly among species.</p></div>","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"32 ","pages":"Pages 73-120"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0065-227X(96)84742-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19753222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microscopic analysis of the elastic properties of connectin/titin and nebulin in myofibrils.","authors":"S Ishiwata, K Yasuda, Y Shindo, H Fujita","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"33 ","pages":"135-42"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19886542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Proteolytic cleavage of connectin/titin","authors":"Atsushi Suzuki , Ken Kim , Yoshihide Ikeuchi","doi":"10.1016/0065-227X(96)81663-7","DOIUrl":"10.1016/0065-227X(96)81663-7","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"33 ","pages":"Pages 53-64"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0065-227X(96)81663-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53633103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of connectin/titin and nebulin in striated muscles of chicken.","authors":"Y Shimada, M Komiyama, S Begum, K Maruyama","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"33 ","pages":"223-33"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19886550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"cDNA sequence of rabbit cardiac titin/connectin.","authors":"M L Greaser, M G Sebestyen, J D Fritz, J A Wolff","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"33 ","pages":"13-25"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19886651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Robert Horowits, Gang Luo, Jian Q. Zhang, Amy H. Herrera
{"title":"Nebulin and nebulin-related proteins in striated muscle","authors":"Robert Horowits, Gang Luo, Jian Q. Zhang, Amy H. Herrera","doi":"10.1016/0065-227X(96)81670-4","DOIUrl":"10.1016/0065-227X(96)81670-4","url":null,"abstract":"","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"33 ","pages":"Pages 143-150"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0065-227X(96)81670-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53633385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}