Agrociencia最新文献_第9页

REDUCTION OF CYANIDE CONTENT IN PHYLOSTACHIS AUREA BAMBOO SHOOTS: OPTIMIZING THE PROCESSING CONDITIONS OF TEMPERATURE, HEATING TIME, AND NaCl CONCENTRATION 降低金莲竹笋中氰化物含量:优化温度、加热时间和NaCl浓度

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-02-15 DOI: 10.47163/agrociencia.v57i1.2441

Norma Abril Borja-Zamora, Mauricio Mora-Tello, Gabriel Abraham Cardoso Ugarte

引用次数: 0

Artificial neural network models for prediction of standardized precipitation index in central Mexico 墨西哥中部标准化降水指数预测的人工神经网络模型

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-02-10 DOI: 10.47163/agrociencia.v57i1.2655

R. Magallanes-Quintanar, C. Galván-Tejada, J. Galván-Tejada, S. J. Méndez-Gallegos, F. Blanco-Macías, R. Valdez-Cepeda

{"title":"Artificial neural network models for prediction of standardized precipitation index in central Mexico","authors":"R. Magallanes-Quintanar, C. Galván-Tejada, J. Galván-Tejada, S. J. Méndez-Gallegos, F. Blanco-Macías, R. Valdez-Cepeda","doi":"10.47163/agrociencia.v57i1.2655","DOIUrl":"https://doi.org/10.47163/agrociencia.v57i1.2655","url":null,"abstract":"Some of the effects of climate change may be related to a change in patterns of rainfall intensity or rainfall scarcity. So, humanity is facing environmental challenges due to an increase in the occurrence of droughts. Forecasting of droughts based on cumulative influence of rainfall could be greatly beneficial for mitigating adverse consequences on water-sensitive sectors such as agriculture. Then, predictive models of drought indices could help in assessing water scarcity situations, droughts identification and their severity characterization. In this paper, we tested the feasibility of the Artificial Neural Network as a data-driven model for predicting the monthly Standardized Precipitation Index in 4 regions (Semi-desert, Highlands, Canyons and Mountains) of north-central México using predictive variable data from 1965 to 2004 as training data and simulated data for the period 2005-2014. The best model was found using the Hyperbolic Tangent as activation function and the Adaptive Moment Estimation (Adam) algorithm as optimization method. The best model was set to the following architecture: 26-12-1 network with 4 weights and 365 trainable parameters. Based on analysis of scatter plot between predicted and observed Standardized precipitation Index values for the test dataset, the Coefficient of Determination was between 0.84 and 0.88. In terms of quantitative statistics averaged over the test set, Artificial Network Model performed very well in predicting Standardized Precipitation Index at the four analyzed regions. This was verified by all-region average value of performance statistics Mean Absolute Error (0.0805), Mean Square Error (0.0144) and the Coefficient of Determination (0.8671). In a nutshell we summarize that the Artificial Network models developed and tested in this study had good prediction skills of the monthly Standardized Precipitation Index for stations and its drought-related properties in the study region.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79531276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PROXIMAL, THERMAL, AND STRUCTURAL CHARACTERIZATION OF STARCH EXTRACTED FROM TWO VARIETIES OF WHITE SORGHUM CULTIVATED IN MEXICO 从墨西哥栽培的两种白高粱中提取的淀粉的近端、热和结构特征

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-02-10 DOI: 10.47163/agrociencia.v57i1.2641

Jared Reyes Gallardo, Guadalupe C. Rodriguez Castillejos, Ricardo Navarro Cortez, Adriana Perales Torres, San Juana Alemán Castillo, Octelina Castillo Ruíz

{"title":"PROXIMAL, THERMAL, AND STRUCTURAL CHARACTERIZATION OF STARCH EXTRACTED FROM TWO VARIETIES OF WHITE SORGHUM CULTIVATED IN MEXICO","authors":"Jared Reyes Gallardo, Guadalupe C. Rodriguez Castillejos, Ricardo Navarro Cortez, Adriana Perales Torres, San Juana Alemán Castillo, Octelina Castillo Ruíz","doi":"10.47163/agrociencia.v57i1.2641","DOIUrl":"https://doi.org/10.47163/agrociencia.v57i1.2641","url":null,"abstract":"Sorghum (Sorghum bicolor) is a starch source that may contain tannins in red varieties, so white grains are preferred as a better alternative. The hypothesis of this study was that white sorghum starch grown in Mexico has the necessary properties for industrial use. The objective was to evaluate the chemical, thermal and structural characteristics of starch extracted from white sorghum var. Mazatlan-16, and RB-Paloma. Protein, moisture, ash, fiber, fat, water absorption rate, and swelling capacity were evaluated. Thermal properties were determined by calorimetry and viscosity analysis; structural properties by Fourier transform infrared spectroscopy and X-ray diffraction. One-way analysis of variance and Student’s t-test (p ≤ 0.05) were performed. The starch extracted from Mazatlán-16 grain contained the highest moisture (5.47 %), protein (0.72 %) and crude fiber values, and no significant differences (p ≤ 0.05) were found for ash and fat content in both starch varieties. The water absorption rate and swelling power was higher for RB-Paloma variety starch (p ≤ 0.05). Regarding thermal properties, the maximum viscosity temperature was 80.55 and 89 °C for RB-Paloma and Mazatlan-16 starch, respectively; the peak gelatinization temperature was higher for Mazatlan-16 (72.28 °C) and showed differences (p ≤0.05) with RB-Paloma. Structural analysis revealed that the starches of both varieties correspond to the A-type crystalline pattern, with the presence of OH, CH in stretching, H2O and CH2 groups. The starches extracted from the two varieties showed properties suitable for use as a viable alternative in the food industry.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86020641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DESCRIPTIVE STUDY OF THE CAPACITY OF SIX HILLSIDE SOIL MANAGEMENT SYSTEMS IN THE CONTROL OF SURFACE RUNOFF 六种山坡土壤管理系统控制地表径流能力的描述性研究

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-01-30 DOI: 10.47163/agrociencia.v57i1.2408

Rigoberto Zetina Lezama, Sergio URIBE GOMEZ, Angel CAPETILLO BURELA, Nestor FRANCISCO NICOLAS, Carmen Aridai HERNANDEZ ESTRADA, Marco Antonio Reynolds-Chávez

{"title":"DESCRIPTIVE STUDY OF THE CAPACITY OF SIX HILLSIDE SOIL MANAGEMENT SYSTEMS IN THE CONTROL OF SURFACE RUNOFF","authors":"Rigoberto Zetina Lezama, Sergio URIBE GOMEZ, Angel CAPETILLO BURELA, Nestor FRANCISCO NICOLAS, Carmen Aridai HERNANDEZ ESTRADA, Marco Antonio Reynolds-Chávez","doi":"10.47163/agrociencia.v57i1.2408","DOIUrl":"https://doi.org/10.47163/agrociencia.v57i1.2408","url":null,"abstract":"On tropical hillsides, torrential rains cause surface runoff that removes soil particles, nutrients and agro-inputs. This process limits soil fertility, agrosystem productivity and the rural economy. A descriptive study was conducted on a hillside in Los Tuxtlas, Veracruz, Mexico, to study water runoff and its runoff coefficients in four modalities of the “milpa intercropped with fruit trees” (MIAF) system, traditional and zero tillage, under rainfed conditions. The systems were established with a systematic design of treatments, without repetitions, between 2003 and 2011: 1) Milpa intercropped in chicozapote (Manilkara zapota), with sediment filter and minimum tillage (MIAF-CH-CF-LM); 2) Milpa intercropped with Persian lemon (Citrus x latifolia), with sediment filter and minimum tillage (MIAF-L-CF-LM); 3) Milpa intercropped in carambolo (Averrhoa carambola), with sediment filter and minimum tillage (MIAF-C-CF-LM); 4) Milpa intercropped in carambolo, without sediment filter and zero tillage (MIAF-C-SF-L0); 5) Zero tillage with crop residue distribution over the surface (Lo); and 6) Minimum tillage with crop residue burning and tracking (LM-QR). From August 2016 to February 2018, a 50 m2 runoff lot was installed in each system. Surface runoff was measured every 24 h; runoff sheet and runoff coefficient. The MIAF.system reduced surface runoff by 46.13 % with respect to that obtained in LM-QR. The MIAF systems with sediment filter and zero tillage without filter presented similar runoff and runoff coefficient values. The MIAF-CH-CF-LM system recorded the lowest runoff value among systems with tillage and filter. The L0 system showed the lowest volume and runoff coefficient.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90605934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POST-HARVEST CHARACTERISTICS OF MANGO FRUITS (Mangifera sp.) FROM SOCONUSCO, CHIAPAS 恰帕斯州SOCONUSCO地区芒果果实(Mangifera sp.)的采后特征

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-01-27 DOI: 10.47163/agrociencia.v57i1.2546

Berenice Karina Flores-Hernández, M. L. Arévalo-Galarza, Rubén San Miguel-Chávez, S. Espinosa-Zaragoza, Noé Delfino Pérez-Díaz, V. H. Aguilar-Rincón

{"title":"POST-HARVEST CHARACTERISTICS OF MANGO FRUITS (Mangifera sp.) FROM SOCONUSCO, CHIAPAS","authors":"Berenice Karina Flores-Hernández, M. L. Arévalo-Galarza, Rubén San Miguel-Chávez, S. Espinosa-Zaragoza, Noé Delfino Pérez-Díaz, V. H. Aguilar-Rincón","doi":"10.47163/agrociencia.v57i1.2546","DOIUrl":"https://doi.org/10.47163/agrociencia.v57i1.2546","url":null,"abstract":"Mango (Mangifera indica L.) is the second most important tropical fruit in the world. In the Soconusco region of Chiapas, Mexico, there is a large number of mango genotypes with a broad range of shapes, flavors, and aromas, with the potential to increase its cultivation. Therefore, the post-harvest characteristics of three creole mango genotypes, ‘Manililla’, ‘Cuero’, and ‘Manzana’ were evaluated and compared with the variety ‘Ataúlfo’. Fifty fruits from each genotype were harvested and stored at 20 °C and 75 % relative humidity; quality characteristics (epidermis/seed/pulp ratio, weight loss, color, firmness, °Bx, titratable acidity, ascorbic acid, pectimethylesterase enzymatic activity, phenolic acid and flavonoid content in epidermis) were evaluated under a completely randomized design. The results showed that ‘Manililla’ and ‘Cuero’ fruits had a significantly higher weight loss, while ‘Manzana’ and ‘Ataúlfo’ were similar in pulp content. The creole genotypes ‘Cuero’ and ‘Manzana’ had an attractive yellow-orange color, with red shading on the shoulders and orange in the pulp. The SST/AT ratio in ‘Manililla’ and ‘Cuero’ (71.1 and 62.6, respectively), was significantly higher in relation to ‘Ataúlfo’ (39.1) due to low acidity. ‘Manzana’ had higher total sugar content (30.3 g 100 g-1) and a firmer texture after six days of storage (dda). ‘Cuero’ showed greater weight loss and higher ascorbic acid content, 32 % more than ‘Ataúlfo’. The pulp content of ‘Manzana’ fruits was similar to that of ‘Ataúlfo’, with less weight loss and greater firmness, making it appealing for marketing.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80617619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHALLENGES AND MANAGERIAL STRATEGIES OF COFFEE COOPERATIVES FROM THE HUATUSCO REGION IN MEXICO: A LEADERS' PERSPECTIVE 墨西哥华图斯科地区咖啡合作社的挑战与管理策略:领导者的视角

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-01-19 DOI: 10.47163/agrociencia.v56i8.2741

Margarita Velandia, C. Trejo-Pech, Benigno Rodríguez-Padrón, R. Servín-Juárez, C. Stripling

{"title":"CHALLENGES AND MANAGERIAL STRATEGIES OF COFFEE COOPERATIVES FROM THE HUATUSCO REGION IN MEXICO: A LEADERS' PERSPECTIVE","authors":"Margarita Velandia, C. Trejo-Pech, Benigno Rodríguez-Padrón, R. Servín-Juárez, C. Stripling","doi":"10.47163/agrociencia.v56i8.2741","DOIUrl":"https://doi.org/10.47163/agrociencia.v56i8.2741","url":null,"abstract":"Coffee cooperatives from the Huatusco region in Mexico have played an important role in helping coffee farmers in this region maintain economic viability in the midst of Mexico’s economic crisis and the international crisis of the coffee sector over time. While these types of organizations have played an important role, not all of these organizations have survived over time. Using information from interviews with leaders of five longeval coffee producer cooperatives in the Huatusco coffee region, this study tries to identify factors that could explain the longevity and long-term sustainability of these organizations. Additionally, using information from these interviews, we evaluate challenges that could put at risk the long-term sustainability of these associations and strategies to overcome these challenges from the cooperative’s leadership perspective. Findings from this study suggest the motivations behind the creation of these producer associations that goes beyond accessing government resources, an institutional structure where there is a democratic process behind the election of leaders, leadership positions have a definite term, and association strategies to increase member engagement could explain the longevity of these organization. Regarding challenges the cooperatives would be facing in the future, the aging population of growers is perceived by cooperative leaders as one of the major challenges they will be facing in the future. Increasing youth involvement in the cooperatives through training and educational activities was perceived by cooperative leaders as a strategy to overcome this challenge.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81783469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GRAIN YIELD STABILITY AND BIOCHEMICAL NUTRIENT CONTENT IN WHITE CORN EXPERIMENTAL CROSSES 白玉米试验杂交籽粒产量稳定性及生化养分含量

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-01-16 DOI: 10.47163/agrociencia.v56i8.2423

F. Sánchez-Ramírez, M. Mendoza-Castillo, C. G. Mendoza-Mendoza, A. Delgado-Alvarado, Etzael Nuñez-Terrones

{"title":"GRAIN YIELD STABILITY AND BIOCHEMICAL NUTRIENT CONTENT IN WHITE CORN EXPERIMENTAL CROSSES","authors":"F. Sánchez-Ramírez, M. Mendoza-Castillo, C. G. Mendoza-Mendoza, A. Delgado-Alvarado, Etzael Nuñez-Terrones","doi":"10.47163/agrociencia.v56i8.2423","DOIUrl":"https://doi.org/10.47163/agrociencia.v56i8.2423","url":null,"abstract":"In Mexico, about half of white corn production is used for human consumption. In a breeding program, in addition to increasing the yield and genotypes stability, the aim is to improve grain quality. Assuming that there is genetic variation among the elite genotypes evaluated in this research, at least one genotype was considered to be outstanding for its high productive potential and desirable characteristics for grain quality. The objective of this work was to determine the genetic variation present in experimental hybrids in terms of yield potential and grain quality, as well as to identify the most outstanding genotypes. In 2016, twenty white corn experimental crosses were evaluated under a randomized block design with three replications in two locations of the ecological area of the High Valleys of Mexico and one location in a transitional area. Yield stability was determined using the additive main effects and multiplicative interaction model. Grain quality was evaluated through starch, protein and oil content. Most of the crosses had higher yield and stability than the controls (commercial hybrids). Likewise, nutrient content varied among them for each biochemical compound; oil concentration ranged from 3.5 to 6.4 g 100 g-1 dry matter (ms); protein, from 5.5 to 9.8 g 100 g-1 ms; and starch, from 72.6 to 96.6 g 100 g-1 ms. Based on their phenotypic stability, yield and grain quality, we observed that crosses 1213, 1217, 1218, 1314, 1418, and 1812 were outstanding, which can be used to produce grain with promising starch and oil content. In general, we infer that the crosses showed potential for starch utilization and tortilla production.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88941278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GRAZING BEHAVIOR OF NEW ZEALAND HOLSTEIN COWS WITH ACCESS TO SHADE 有树荫的新西兰荷斯坦奶牛的放牧行为

IF 0.3 4区农林科学

Agrociencia Pub Date : 2023-01-11 DOI: 10.47163/agrociencia.v56i8.2446

Marisol Ortiz-Texon, Rodolfo Ramírez-Valverde, M. E. Ortega-Cerrilla, Lucía Améndola-Saavedra, J. Burgueño-Ferreira, R. Améndola-Massiotti

{"title":"GRAZING BEHAVIOR OF NEW ZEALAND HOLSTEIN COWS WITH ACCESS TO SHADE","authors":"Marisol Ortiz-Texon, Rodolfo Ramírez-Valverde, M. E. Ortega-Cerrilla, Lucía Améndola-Saavedra, J. Burgueño-Ferreira, R. Améndola-Massiotti","doi":"10.47163/agrociencia.v56i8.2446","DOIUrl":"https://doi.org/10.47163/agrociencia.v56i8.2446","url":null,"abstract":"Access to shade decreases heat stress of grazing dairy cows, but may reduce their grazing time. It was hypothesized that access to shade would alter grazing behavior of New Zealand Holstein cows. The objective was to evaluate the distribution of time use of cows with and without access to shade in a temperate sub-humid climate with summer rainfall, where the mean annual temperature varies between 12 (January) and 18 °C (May). During the warm (May) and cool (October) seasons of 2017, two lots of nine lactating New Zealand Holstein cows grazed in two treatments, with (S) and without (NS) shade access. Behavior was recorded every 10 min for 5 d per period. The response variables were: grazing times; rumination standing, prostrate and total; resting standing, prostrate and total. The data were classified as diurnal (07:00 to 19:00 h) and nocturnal (19:00 to 07:00 h); the former were grouped into four shifts according to Temperature and Humidity Index (TIH) and solar radiation. It was analyzed with a general linear model and the MIXED procedure. In the warm season (19 °C. precipitation of 44.3 mm) cows with access to shade grazed 16 % less time (p ≤ 0.05) in the shifts of higher THI and solar radiation, being higher the percentage of shade use. During the cool season (16 °C, precipitation 62.0 mm) at times of higher THI (71.0) and solar radiation (880.5 Wm-2) S cows ruminated standing 44 % longer (p ≤ 0.05), with total rumination time 30.7 % longer (p ≤ 0.05) than NS; the latter rested standing 22.5 % longer (p ≤ 0.05). Access to shade modified the behavior of New Zealand Holstein cows grazing in the temperate climate of Mexico.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81083735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFFECT OF TEMPERATURE AND ULTRAVIOLET RADIATION ON GROWTH AND PATHOGENICITY OF Metarhizium anisopliae 温度和紫外线辐射对绿僵菌生长和致病性的影响

IF 0.3 4区农林科学

Agrociencia Pub Date : 2022-12-20 DOI: 10.47163/agrociencia.v56i8.2477

María Elena Márquez-Gutiérrez, Evelyn Pérez-Lezcano, Y. Baró-Robaina, Francisco Paúl Gámez Vázquez, C. Aguirre-Mancilla, Gerardo Armando Aguado Santacruz

{"title":"EFFECT OF TEMPERATURE AND ULTRAVIOLET RADIATION ON GROWTH AND PATHOGENICITY OF Metarhizium anisopliae","authors":"María Elena Márquez-Gutiérrez, Evelyn Pérez-Lezcano, Y. Baró-Robaina, Francisco Paúl Gámez Vázquez, C. Aguirre-Mancilla, Gerardo Armando Aguado Santacruz","doi":"10.47163/agrociencia.v56i8.2477","DOIUrl":"https://doi.org/10.47163/agrociencia.v56i8.2477","url":null,"abstract":"The efficacy of entomopathogenic fungi in the field depends on the influence of various environmental factors, including temperature and level of exposure to UV light. It is hypothesized that temperature and level of UV light exposure of Metarhizium anisopliae (Hypocreales, Clavicipiceae) may affect its efficiency in controlling the sweetpotato weevil (Cylas formicarius, Fabricius; Coleoptera, Brentidae). The objective of this study was to evaluate the influence of temperature and ultraviolet light radiation on the growth and pathogenicity of different native strains of Cuban origin of the M. anisopliae Sorokin complex with biological activity against C. formicarius. Colony diameter was measured at 28, 30, 32 and 34 °C temperature and exposed to ultraviolet light (254 nm) for 10, 15, 20, 30, 30, 60 and 120 minutes. Under laboratory conditions, the percentage germination of conidia of strains LBMa-11, LBM-30, LBM-41 and LBM-146 was determined, as well as their pathogenic capacity by means of bioassays with adults of C. formicarius in terms of the percentage of cumulative mortality and the mean lethal time (TL50). Results proved that temperature and UV light affect the ability of M. anisopliae to control C. formicarius. A delay in cumulative mortality was detected for all strains exposed to UV light, with an increase in TL50. The optimum temperature range was between 28 and 30 °C. The strain LBM-127 showed the highest sensitivity to temperature, LBMa-11 the highest tolerance to UV light, and LBM-146 was the most virulent according to Probit regression analysis.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83794281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NATIVE BACTERIA IN RASPBERRY CROWN GALL REDUCE THE SEVERITY OF Agrobacterium tumefaciens 树莓树冠瘿中的原生细菌可降低农杆菌的严重程度

IF 0.3 4区农林科学

Agrociencia Pub Date : 2022-12-15 DOI: 10.47163/agrociencia.v56i8.2871

Elizabeth Sánchez Jiménez, Sergio Aranda Ocampo, Daniel L. Ochoa Martínez, Dimas Mejiía Sánchez

{"title":"NATIVE BACTERIA IN RASPBERRY CROWN GALL REDUCE THE SEVERITY OF Agrobacterium tumefaciens","authors":"Elizabeth Sánchez Jiménez, Sergio Aranda Ocampo, Daniel L. Ochoa Martínez, Dimas Mejiía Sánchez","doi":"10.47163/agrociencia.v56i8.2871","DOIUrl":"https://doi.org/10.47163/agrociencia.v56i8.2871","url":null,"abstract":"Native bacterial populations in crown galls caused by Agrobacterium tumefaciens may harbour bacteria of interest for biocontrol of this pathogen. In this study, we explored the density of native bacterial populations in crown galls of raspberry (Rubus ideaus) and evaluated their in vitro and in vivo antagonism against A. tumefaciens. Bacteria morphologically similar to A. tumefaciens were isolated from six gall samples and identified by virD2 gene sequencing. Bacterial population density was calculated by direct plate count on nutrient agar and R2A media. The in vitro antagonism efficiency index against A. tumefaciens of the most frequent bacteria was evaluated by dual confrontation on nutrient agar medium, and in vivo by inoculation of 1.5 x 108 UFC mL-1 in the root of tomato (Solanum lycopersiucm) plants under greenhouse conditions. By direct sequencing and biovar characterization, it was identified as A. tumefaciens biovar 1 in raspberry galls. Native bacterial populations in galls have variable density and their diversity is limited. By partial amplification of the 16S rRNA gene, 13 strains were identified with the highest frequency in the genera Pseudomonas (61.5 %), Bacillus (15.3 %), Alcaligenes (15.3 %) and Delftia (7.6 %). Among these, Alcaligenes faecalis showed the highest in vitro antagonism index (p ≤ 0.05) against A. tumefaciens, followed by Delftia sp. and Pseudomonas citronellolis. In vivo inoculation of tomato plants with these antagonists against Agrobacterium tumefaciens did not prevent infection; however, Alcaligenes faecalis significantly reduced (p ≤ 0.05) the severity of plant stem tumours. A. faecalis is the most efficient antagonist in vitro and in vivo against A. tumefaciens.","PeriodicalId":50836,"journal":{"name":"Agrociencia","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85179041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0