Paloma Helena Sanches da Silva, Pedro Antônio Bronhara Pimentel, Luana Caetano Machado, Nathalia Estevão Caixeta, Paula Izabella Alves da Rocha, Bruno Ferrante, Patrícia Maria Coletto Freitas, Rodrigo Dos Santos Horta
{"title":"Bronchopulmonary Segmentation in Humans and Dogs: A Comparative Narrative Review Highlighting Anatomical Gaps and Surgical Implications.","authors":"Paloma Helena Sanches da Silva, Pedro Antônio Bronhara Pimentel, Luana Caetano Machado, Nathalia Estevão Caixeta, Paula Izabella Alves da Rocha, Bruno Ferrante, Patrícia Maria Coletto Freitas, Rodrigo Dos Santos Horta","doi":"10.1111/ahe.70113","DOIUrl":"10.1111/ahe.70113","url":null,"abstract":"<p><p>Bronchopulmonary segmentation (BS) is an anatomical concept, poorly described in veterinary medicine, and involve a specific segmental bronchus, a corresponding segmental artery, segmental veins draining into intersegmental veins, and intersegmental septa. While pioneer studies were comparative in humans and domestic animals, this research path diverged decades ago, and the field has since lacked a proper, functional categorization of canine lungs into bronchial segments. Veterinary anatomical nomenclature does recognize this segmentation, but the classification is traditionally based on tertiary (or segmental) bronchi and does not specify how many segments each pulmonary lobe may have. A comparative analysis of the scarce literature suggests that the canine species possesses the necessary anatomical requirements for this classification. These include a segmental bronchus, a corresponding segmental artery, segmental veins draining into intersegmental veins, and intersegmental septa containing connective tissue. Further studies are needed to better detail the variation in pulmonary structures, particularly the venous drainage that defines BS. Specific anatomical ratios and relations should also be described in relation to a dog's size, breed, and sex. Although segmentectomy has seemed distant for veterinary medicine, these findings and future studies may bring its use closer to a reality, similar to its established role in medicine.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70113"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Abumandour, Eman Kamal Khalil, Diaa Massoud, Mustafa Shukry, Neveen El-Bakary
{"title":"Visual Adaptations of the Dicentrarchus punctatus: New Insights Into Its Retina Adaptations With Transmission Electron Microscopy Analysis.","authors":"Mohamed Abumandour, Eman Kamal Khalil, Diaa Massoud, Mustafa Shukry, Neveen El-Bakary","doi":"10.1111/ahe.70122","DOIUrl":"10.1111/ahe.70122","url":null,"abstract":"<p><p>This study was designed to illustrate the light and electron microscopic characterisations of the eye of Dicentrarchus punctatus with new insight into transmission electron microscopic features of the retina. The eye is composed of three layers: the external sclera-cornea layer, the middle layer, and the internal layer. The cornea has three laminae: stratified epithelial cuboidal cells anteriorly, Bowman (middle), and the dermato-stromal lamina posteriorly. The mucoidal lamina was observed between the cranial part of the sclera-corneal and the derma-stromal, and established by the fibre bundles of the collagen implanted in the loose connective tissue, while the iridescent lamina was found between the cranial and the caudal sclera-stromal lamina. SEM observation revealed that the cornea consisted of rounded or ovoid-shaped cells that were joined to each other by microridges. The retina is consisted of ten layers. The retina had two regions: nervous and non-nervous. The retina had both rods and cones (single, double, and triple) photoreceptor cells. The inner segment of the rod had numerous large, round mitochondria with electron-dense granules and melanin pigment extended between the ellipsoid units, while the outer segment had stacks of discs. The cone's inner segments had ellipsoids that showed mitochondria with electron-dense granules and melanin pigment extended between the ellipsoid units, in addition to disintegrated cristae. Finally, the double and triple cone cells with ellipsoids played a key role in extending the light's accessible absorption area.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70122"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Motoharu Oishi, Yuuki Yamashita, Rei Takahata, Nobutsune Ichihara
{"title":"Inter-Individual Variations in Radial Bowing in Beagles: Cranial and Medial Deviation Patterns.","authors":"Motoharu Oishi, Yuuki Yamashita, Rei Takahata, Nobutsune Ichihara","doi":"10.1111/ahe.70118","DOIUrl":"https://doi.org/10.1111/ahe.70118","url":null,"abstract":"<p><p>The canine radius has anatomical and clinical importance because its shape influences forelimb alignment, joint orientation, and load distribution. However, normal three-dimensional variations in radial bowing within a single breed have not been sufficiently described. This study aimed to characterise inter-individual variations in radial bowing in Beagles using three-dimensional morphometry and principal component analysis. Nineteen radial specimens from 19 Beagles were examined. Surface morphology was captured using a three-dimensional scanner, with reference points placed on the proximal and distal articular regions and at three shaft levels corresponding to 25%, 50%, and 75% of the radial length. Distances from each shaft centroid to the radial axis, sagittal plane, and dorsal plane were measured and normalised for radial length, and principal component analysis was performed using the normalised variables. The middle level of the radius showed the greatest deviation among the three shaft levels. Cranial deviation was more pronounced than mediolateral deviation, and mediolateral deviation in the dorsal plane was directed medially. Principal component analysis showed that the first two principal components explained more than 90% of the total variance. The first principal component mainly reflected cranial deviation at the middle level of the radius, whereas the second principal component reflected medial deviation at the proximal and middle levels. These findings quantitatively demonstrate inter-individual variations in radial bowing among Beagles and provide a three-dimensional anatomical basis for future studies on radial morphology, joint orientation, and orthopaedic assessment in canines.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70118"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147788258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabriel Soares Tosatto Pellenz, Gabriel Navarrina Soares, Marcelo José Böck, Vitor Pires Pereira, William Soares Teixeira, Sérgio Carvalho, Sokol Duro, William Pérez, Luciano de Morais-Pinto
{"title":"Olive Waste as Feed Supplement: Morphometrical Effects on the Ansa spiralis coli in Lambs.","authors":"Gabriel Soares Tosatto Pellenz, Gabriel Navarrina Soares, Marcelo José Böck, Vitor Pires Pereira, William Soares Teixeira, Sérgio Carvalho, Sokol Duro, William Pérez, Luciano de Morais-Pinto","doi":"10.1111/ahe.70114","DOIUrl":"https://doi.org/10.1111/ahe.70114","url":null,"abstract":"<p><p>Feedlot systems for small ruminants require nutritionally adequate and viable diets, and the incorporation of olive residues can offer a sustainable and economically advantageous alternative. This study evaluated the effects of including olive waste at 7.5%, 15%, 22.5% and 30% in the diets of Texel × Ile-de-France lambs, assessing the architecture and structure of the Ansa spiralis coli through gross anatomy, morphometry and light microscopy using ImageJ software (Fiji). Diets with up to 22.5% olive residue did not modify the morphology or morphometric parameters of the Ansa spiralis coli, with all specimens exhibiting three centripetal and three-and-a-half centrifugal gyri and a mean length ratio of 1.18; however, the 30% inclusion level significantly increased the thickness of the tunica muscularis, suggesting potential effects on intestinal motility. These findings indicate that up to 22.5% olive residue can be safely incorporated into lamb feed as a sustainable and economically viable option, helping bridge knowledge gaps on olive byproduct use in ruminant nutrition while offering practical solutions for producers seeking to optimize regional feed resources and maintain animal health and productivity.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70114"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147788265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alaa I Abd El Latif, Ashraf A El Sharaby, Eman M Hagras, Ahmed G Nomir
{"title":"Vincristine-Induced Craniofacial Skeletal Teratogenicity in Foetal Rabbits: Multimodal Morphometric Assessment and Partial Protection by L-Carnitine.","authors":"Alaa I Abd El Latif, Ashraf A El Sharaby, Eman M Hagras, Ahmed G Nomir","doi":"10.1111/ahe.70120","DOIUrl":"https://doi.org/10.1111/ahe.70120","url":null,"abstract":"<p><p>Vincristine, a microtubule-disrupting vinca alkaloid, is teratogenic during organogenesis and may compromise neural crest-related craniofacial development; however, its effects on craniofacial and cervical development and the potential for maternal L-carnitine protection remain unclear. Pregnant New Zealand White rabbits (n = 9) were allocated to Group I-Control (saline), Group II-Vincristine (0.1 mg/kg intraperitoneally [IP] on gestational day [GD] 10) and Group III-Vincristine + L-carnitine (vincristine 0.1 mg/kg IP on GD 10 plus L-carnitine 500 mg/kg IP on GD 9-14). Foetuses were collected at GD 29 for craniofacial and cervical morphometry, radiographic measurements and Alcian blue/Alizarin red S skeletal staining. Compared with Group I controls, vincristine caused 36.7% embryonic resorption, universal craniofacial malformations, severe intrauterine growth retardation, 33%-85% reductions in craniofacial dimensions relative to control values, 29%-48% decreases in radiographic skull and mandibular measures and 22.8%-57.5% shortening of cervical metrics (all p < 0.001). With L-carnitine co-administration, resorption decreased to 13.3% and gross malformation burden was reduced; quantitatively, L-carnitine recovered approximately 53%-78% of the vincristine-induced craniofacial deficit, 65%-100% of the radiographic skull and mandibular deficit and 71.5%-79% of the cervical shortening, although all measurements except rostral skull width remained significantly different from controls (p < 0.05). These findings indicate that maternal L-carnitine provides substantial, but incomplete, attenuation of vincristine-associated craniofacial and cervical teratogenicity in the rabbit model.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70120"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Beta Defensins in the Host Defense of Sheep Tongues: Cellular Localization and Possible Roles.","authors":"Bayram Bayram, Fatma Çelenk","doi":"10.1111/ahe.70123","DOIUrl":"https://doi.org/10.1111/ahe.70123","url":null,"abstract":"<p><p>In domestic animals, a diverse range of antimicrobial peptides function as natural barriers to microbial invasion. Among these, defensins small, cationic, cysteine-rich, amphipathic peptides represent key elements of the innate immune system in multicellular organisms. The present study aimed to investigate the presence, regional localisation, and cellular distribution of β-defensin 1 (BD1), β-defensin 2 (BD2), β-defensin 3 (BD3), and β-defensin 4 (BD4), members of the defensin family, in the tongues of adult sheep using immunohistochemical techniques. BD1 and BD2 exhibited weak to moderate immunoreactivity, primarily localized to the basal and parabasal layers of the lingual epithelium, whereas BD3 and BD4 displayed strong expression across the entire epithelial layer. All of the defensins examined showed higher expression levels in the epithelial cells of filiform and lenticular papillae. Furthermore, variable expression of BD1, BD2, BD2, and BD4 was detected in stromal cells, skeletal muscle fibres, and vascular structures. In particular, BD3 and BD4 showed a more intense expression in all tongue tissues. These findings indicate that β-defensins are predominantly expressed in the epithelial compartment of the sheep tongue, suggesting their role in providing rapid local defence against microbial pathogens. The increased expression of BD3 and BD4 suggests that they play a more prominent role in immune responses triggered by inflammatory stimuli.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70123"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paulo de Souza-Junior, Erick Candiota Souza, Wilson Viotto-Souza, Maria Eduarda Rodrigues Costa, Amanda Zanesco Crivelaro, Carlos Benhur Kasper, Marcelo Abidu-Figueiredo
{"title":"Beyond Domestication: Occurrence of Levator Anguli Oculi Medialis and Retractor Anguli Oculi Lateralis in Four Neotropical Canid Species.","authors":"Paulo de Souza-Junior, Erick Candiota Souza, Wilson Viotto-Souza, Maria Eduarda Rodrigues Costa, Amanda Zanesco Crivelaro, Carlos Benhur Kasper, Marcelo Abidu-Figueiredo","doi":"10.1111/ahe.70121","DOIUrl":"https://doi.org/10.1111/ahe.70121","url":null,"abstract":"<p><p>The facial mimetic muscles levator anguli oculi medialis (LAOM) and retractor anguli oculi lateralis (RAOL) have been associated with expressive eye movements in canids and proposed as products of domestication. We investigated their occurrence in four Neotropical species (Lycalopex gymnocercus, Lycalopex vetulus, Cerdocyon thous and Chrysocyon brachyurus). Twelve adult specimens were dissected, and both muscles were consistently identified in all taxa, with conserved architecture. These findings support the interpretation that LAOM and RAOL are not exclusive to domestic dogs and may represent a broadly conserved trait across Canidae.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70121"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Colon Histology and the Presence of CD45- MHC II- CD83+ Follicular Dendritic Cell-Like Cells in the Muscle Coat of the Chicken Colon.","authors":"Ulker Eren, Sadiye Kum, Ozay Gules","doi":"10.1111/ahe.70115","DOIUrl":"https://doi.org/10.1111/ahe.70115","url":null,"abstract":"<p><p>This study aimed to investigate the posthatching histological development of the chicken colon wall and the presence of dendritic cells (DCs) based on CD83, CD45 and MHC II expression. A total of 30 Ross 308 broiler chicks were used, with samples collected throughout the first two posthatching weeks. Crossmon's triple stain and Gordon and Sweet's silver impregnation technique were employed to determine the overall structure of the colon. CD45 and CD83 were assessed in frozen sections, while MHC II was examined in paraffin sections using the streptavidin-biotin complex method. The colon wall became fully organised histologically by the end of the second week. Importantly, to the best of our knowledge, this study is the first to demonstrate the presence of CD83+ cells localised within a restricted band of the chicken colon muscle coat from hatching. These CD83+ cells, expressing neither CD45 nor MHC II, exhibited features consistent with follicular dendritic cell-like cells within the reticular connective tissue of the muscular layer.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70115"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147788297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahmoud Elghoul, Mustafa Shukry, Diaa Massoud, Ramadan Kandyel, Mohamed Abumandour
{"title":"Adaptive Properties of the Podocyte of a Young Domestic Pig (Sus domesticus, Erxleben 1777) Under Histological and Transmission Microscopy Examinations.","authors":"Mahmoud Elghoul, Mustafa Shukry, Diaa Massoud, Ramadan Kandyel, Mohamed Abumandour","doi":"10.1111/ahe.70119","DOIUrl":"https://doi.org/10.1111/ahe.70119","url":null,"abstract":"<p><p>Published data on podocyte morphology in young domesticated pigs are scarce. The current study aimed to characterize the histological and ultrastructural characteristics of the podocyte in Sus domesticus. This study depended on kidney samples from seven healthy two-month-old domestic pigs, which were collected immediately after slaughter and processed for histological and transmission electron microscopy (TEM) examination. The renal cortical tissue consisted of nephron components, including renal corpuscles, proximal convoluted tubules, distal convoluted tubules and collecting tubules. TEM analysis showed that the cytoplasm contained few mitochondria and poorly developed endoplasmic reticulum cisternae. Some podocytes have a primary process that wraps around the basal lamina of the blood capillary and gives rise to small secondary cytoplasmic processes known as pedicels. The podocytes exhibited numerous pedicels resting on the glomerular basement membrane, closely associated with the underlying glomerular endothelium, and projecting towards the capillary lumen. The filtration barrier consisted of fenestrated endothelial cells, a trilaminar glomerular basement membrane and an intact podocyte layer. In conclusion, this study provides a detailed histological and ultrastructural description of podocytes in young Landrace pigs and establishes baseline morphological data that may support future comparative, developmental and functional renal investigations in Sus domesticus.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70119"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mina Motamedi, Fatemeh Shamsaldini, Sana Kheirkhah
{"title":"Morphology and Histology of Major Organ Systems in the Killifish Esmaeilius sophiae (Teleostei: Aphaniidae).","authors":"Mina Motamedi, Fatemeh Shamsaldini, Sana Kheirkhah","doi":"10.1111/ahe.70097","DOIUrl":"https://doi.org/10.1111/ahe.70097","url":null,"abstract":"<p><p>Killifishes have emerged as valuable supplementary biological and biomedical research models due to their remarkable adaptations to extreme and variable environments. Despite their ecological and evolutionary significance, the histology and anatomy of freshwater members of the family Aphaniidae remain largely understudied. This study provides the first comprehensive microscopic analysis of Esmaeilius sophiae, a freshwater killifish endemic to Iran, using histological staining, light microscopy, scanning electron microscopy (SEM) and x-ray microscopy. Key organ systems-including gills, kidneys, liver, brain and reproductive organs-were examined to identify structural adaptations that support survival in dynamic freshwater habitats. The gills possess organised lamellae supported by pillar cells and a specialised mucosal epithelium, enhancing oxygen exchange in environments with fluctuating oxygen availability. SEM imaging revealed specialised glomerular structures in the kidney that likely facilitate efficient osmoregulation, as well as a distinctive scale morphology contributing to environmental protection. Additionally, the well-differentiated brain regions-particularly the optic tectum-and the unique otolith morphology of E. sophiae underscore its sensory and ecological specialisation. Our findings reveal key anatomical and histological adaptations in E. sophiae, establishing a foundational reference for future research in fish physiology, environmental adaptation and evolutionary biology.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":"e70097"},"PeriodicalIF":1.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147788288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}