Anatomia Histologia Embryologia最新文献

{"title":"Determination of Expression and Relationships of Nedd4-Related E3 Ubiquitin Ligase-2 (NEDL2) and Ubiquitin Proteasome System Proteins in Developing Rat Pancreatic Tissue.","authors":"Ilkay Corumluoglu, Ebru Alimogullari, Uygar Sacik","doi":"10.1111/ahe.70176","DOIUrl":"10.1111/ahe.70176","url":null,"abstract":"<p><p>Nedd4-associated E3 ubiquitin ligase 2 (NEDL2) and p97/valosin-containing protein (p97/VCP) are critical regulators of ubiquitin-mediated proteostasis and cellular homeostasis. However, their age-dependent expression patterns in pancreatic tissue during postnatal development and senescent decline remain uncharacterized. This study aimed to investigate the histological alterations alongside the expression profiles of NEDL2 and p97/VCP in rat pancreatic tissue across the postnatal lifespan and ageing. Pancreatic tissues obtained from male Wistar albino rats at postnatal Weeks 1, 4, 10, 36 and 72 were evaluated using histochemical, immunohistochemical and immunofluorescence analyses. Haematoxylin and eosin (H&E) staining were performed to assess morphological changes in endocrine and exocrine pancreas. Immunohistochemistry and semi-quantitative H-score analyses were utilized to evaluate NEDL2 and p97/VCP expression levels. Double immunofluorescence staining was conducted to determine cellular co-localization of NEDL2 with p97/VCP, insulin and glucagon in pancreatic islet cells. Histological analysis showed that the islets of Langerhans progressively enlarged and hypertrophic changes during postnatal ageing, while the exocrine acinar structures maintained its morphological integrity. Immunohistochemical findings revealed dynamic and age-dependent expression of NEDL2 and p97/VCP. Both proteins demonstrated a progressive increase in expression from Week 1 through Week 4. NEDL2 expression peaked significantly at Week 10 (p < 0.001) followed by a gradual decline, whereas p97/VCP expression reached its maximum level at Week 36 (p < 0.001). A significant decrease in expression was observed at Week 72. Immunofluorescence analyses confirmed that NEDL2 strongly co-localized with p97/VCP, insulin-positive β cells and glucagon-positive α cells, particularly during young and mature adulthood (Weeks 10 and 36). Conversely, a marked reduction in fluorescence intensity and co-localization was observed in senescent pancreatic tissue (Week 72). These findings demonstrate that NEDL2 and p97/VCP exhibit coordinated and developmentally regulated expression patterns within pancreatic islet cells. Their elevated expression and prominent co-localization during adulthood suggest a key role for these proteins in pancreatic maturation, endocrine function and cellular proteostasis. Conversely, their down-regulation in senescent tissue may reflect an age-related impairment of protein quality control systems during pancreatic ageing.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 5","pages":"e70176"},"PeriodicalIF":1.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postnatal Cell Type- and Segment-Specific Localization of EGFR and Its Ligands (EGF, TGF-α, and AREG) in the Rat Epididymis.","authors":"Yahy Abood Kareem Alesawi, Emel Ergün","doi":"10.1111/ahe.70174","DOIUrl":"10.1111/ahe.70174","url":null,"abstract":"<p><p>The epididymis is a specialized male reproductive organ that undergoes marked postnatal differentiation and segmental specialization to support sperm maturation, transport and storage. Previous studies have demonstrated the presence of epidermal growth factor receptor (EGFR) and epidermal growth factor (EGF) in the epididymis; however, their comparative postnatal localization, together with that of transforming growth factor-α (TGF-α) and amphiregulin (AREG), across specific epididymal segments and epithelial cell types has not been systematically characterized in the rat. This study investigated the postnatal histomorphological development and cell type- and segment-associated localization of EGFR, EGF, TGF-α and AREG in the epididymis of Wistar albino rats. Epididymal tissues were collected from neonatal (PND5), infantile (PND20), postpubertal (PND50) and adult (PND70) rats (n = 6 per group). Histomorphological development was evaluated using Crossmon's modified trichrome, periodic acid-Schiff (PAS) and toluidine blue staining, whereas immunolocalization was assessed semiquantitatively by immunohistochemistry. Histological examination revealed progressive epithelial differentiation and segment-specific morphological maturation during postnatal development. EGFR immunoreactivity was detected in basal cells, clear cells and periductal smooth muscle cells, with prominent staining in clear cells of the cauda epididymis in postpubertal and adult rats. Immunoreactivity for EGF and TGF-α was restricted to principal, apical and basal cells of the caput epididymis and was detected only during the postpubertal and adult stages. AREG immunoreactivity was observed in basal and clear cells as well as in periductal smooth muscle cells, without statistically significant developmental or segmental differences. Semiquantitative analysis demonstrated significant increases in EGF and TGF-α immunoreactivity in the caput epididymis during the postpubertal and adult stages (adjusted p = 0.043 for both), and immunoreactivity in the caput was significantly higher than that in the corpus and cauda segments (adjusted p = 0.01 for both). No statistically significant differences were detected in EGFR or AREG immunoreactivity. These findings reveal distinct postnatal cell type- and segment-associated localization patterns of EGFR and its ligands. The localization of EGFR and AREG in basal and clear cells, together with the caput-restricted distribution of EGF and TGF-α, provides a descriptive framework for future studies investigating their possible involvement in epididymal epithelial maturation and regional specialization.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 5","pages":"e70174"},"PeriodicalIF":1.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148957282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical Insights Into the Equine Cervical Intervertebral Foramina: Implications for Veterinary Practice.","authors":"Renato Lopes Previdelli, Rupert Frederick Dash, Catrin Sian Rutland, Rachel Tucker, Alex Hawkins","doi":"10.1111/ahe.70154","DOIUrl":"10.1111/ahe.70154","url":null,"abstract":"<p><p>The equine cervical intervertebral foramina (IVF) represent vital anatomical regions facilitating the passage of spinal nerves and associated vasculature. An in-depth understanding of their anatomy is imperative for the safe implementation of diagnostic and surgical interventions. Despite their clinical significance, anatomical descriptions of the equine cervical IVF remain under-characterised in the existing literature, particularly in relation to the needs of equine veterinary surgeons. Our study provides comprehensive anatomical analysis of the IVF at the cervical vertebral levels C5/C6 and C6/C7 in the domestic horse (Equus caballus), targeting advancing clinical methodologies pertinent to cervical foraminotomy procedures. A series of anatomical dissections on equine cadavers' necks was performed to comprehensively evaluate the external musculature and establish contextual orientation, subsequently leading to more intricate dissections and descriptions. This study aimed to undertake descriptive anatomical dissections of the C5/C6 and C6/C7 IVF, employing direct observation of clinical and surgical importance. Data analysis included visual documentation with development of annotated diagrams to elucidate the spatial relationships between skeletal, muscular, neural, and vascular structures within and surrounding the C5/C6 and C6/C7 foramina. The results present a final, sequential, detailed anatomical characterisation of the IVF at the specified cervical levels, highlighting morphological descriptions, structural orientation, and adjacent tissue planes. Critical features relevant to clinical practice are delineated, notably the proximity of neurovascular structures to bony landmarks frequently encountered during surgical interventions. The descriptive anatomy in this study provides novel anatomical interpretations of the equine cervical IVF region and advocates for safer, evidence-based clinical practices in managing conditions that require clinical and surgical interventions.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70154"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13370843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148450467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maturing Rat Adrenal Medulla: 3D Organization and the 'Access Points' Hypothesis for Opposite Distribution Gradients of A- and NA-Cells.","authors":"Konstantin Gerbertovich Kemoklidze, Natalia Andreevna Tyumina, Alexey Vladimirovich Pavlov","doi":"10.1111/ahe.70149","DOIUrl":"https://doi.org/10.1111/ahe.70149","url":null,"abstract":"<p><p>The mature rat adrenal medulla is characterized by a pronounced asymmetry in the 3D organization of chromaffin tissue as a whole and by oppositely directed gradients in the distribution of adrenaline-storing (A-) and noradrenaline-storing (NA-) cells. The aim of this study was to characterize the 3D organization of the rat maturing adrenal medulla. Adrenal glands from 1- to 2-week-old Wistar rats were processed using a highly specific chromaffin reaction according to Honoré, which allows differentiation between A- and NA-cells. A 3D reconstruction of the adrenal medulla was obtained from complete sets of serial sections after A- and NA-cells, blood vessels, nerve fibres, neurons and stroma had been marked on each section. By the time of final differentiation of A- and NA-cells (end of the first postnatal week), the two-level asymmetry of chromaffin tissue characteristic of the mature adrenal medulla and its close association with bundles of nerve fibres was already clearly evident. At the craniomedial margin, where the main nerve bundles enter the medulla, chromaffin tissue formed cones and cords composed almost exclusively of A-cells, whereas towards the caudolateral margin A-cell density decreased and NA-cell density increased. Integrating these data with published information on embryonic migration of sympathoadrenal precursors and on the development of adrenal medullary innervation and vascularization, we propose the 'access points' hypothesis. It proposes that opposite A- and NA-cell gradients result from asynchronous arrest of migrating groups of A- and NA-cell precursors (chromaffinoblasts) as they reach discrete microdomains that provide access to nerve terminals and microvasculature ('access points'), with earlier-settling A-blasts accumulating near the nerve entry zone and later-settling NA-blasts occupying remaining access points.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70149"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148370847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malocclusion as a Trigger for Changes in the Matrix of Articular Cartilage of the Mandible.","authors":"Rebeca Ferreira Badaró, Ludimilla Rocha Dutra Nardotto, Marcelo Dos Santos Bittencourt Filho, Carolina Brioschi Mathias, Willian Grassi Bautz, Leticia Nogueira da Gama-de-Souza","doi":"10.1111/ahe.70151","DOIUrl":"10.1111/ahe.70151","url":null,"abstract":"<p><p>Malocclusion alters the biomechanical environment of the temporomandibular joint (TMJ), potentially affecting the extracellular matrix of the mandibular articular cartilage (ACM). Understanding these changes is essential to elucidate adaptive mechanisms and possible degenerative processes. This study aimed to evaluate proteoglycan deposition and type I collagen (Col I) expression in ACM under experimentally induced malocclusion. Twenty-four 8-week-old female Wistar rats were divided into control and experimental groups with two treatment periods (2 and 4 weeks). Sagittal malocclusions were induced orthodontically, promoting mesial movement of the first molars and distalization of the third molars on opposite sides of the arches. Sagittal sections of ACM were stained with toluidine blue/fast green for proteoglycans and analysed by Col I immunohistochemistry. A reduction in proteoglycans in the territorial zone of the posterior third of the ACM was observed after 2 weeks, followed by new deposition in the 4-week group. Differences in Col I distribution and fibre organization were also observed in the posterior third, particularly in animals treated for 2 weeks. In conclusion, occlusal disorder induced changes in ACM, particularly in the early stages of overload, suggesting tissue remodelling associated with altered functional demands.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70151"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148406656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined CT and Rodopas Casting Assessment of Postural Variations in Lung Morphology of European Pond Turtles (Emys orbicularis).","authors":"Omid Zehtabvar, Ali Reza Vajhi, Hesameddin Akbarein, Somaye Davudypoor, Seyyed Hossein Modarres Tonekabony, Navid Chavoshi Tehrani, Muhammad Shahbazi Dastjerdi","doi":"10.1111/ahe.70125","DOIUrl":"10.1111/ahe.70125","url":null,"abstract":"<p><p>Respiratory morphology in chelonians exhibits unique adaptations compared to mammals, with multicameral lungs and large terminal chambers. This study combined computed tomography (CT) imaging and Rodopas-casting to assess structural and volumetric changes in the lungs of adult male European pond turtles (Emys orbicularis) between extended and flexed postural states of the head, neck, and limbs. Five turtles (550 ± 10.22 g) were evaluated under anaesthesia. CT scans quantified lung dimensions, volume, parenchymal thickness, and percentage coelomic occupancy, while Rodopas casts provided detailed bronchial architecture. CT imaging revealed bilateral wedge-shaped lungs positioned dorsally in the coelomic cavity, with the trachea entering from the right side and bifurcating into a longer left and shorter right bronchus. Each lung contained three lateral and four medial secondary bronchi, terminating in sac-like chambers, plus a large caudal terminal chamber. Lateral chambers were larger than medial ones; tertiary bronchi were denser in cranial lung regions. Casting confirmed bronchial branching but showed smaller terminal sac volume than CT, likely due to post-mortem differences. Morphometric analysis showed no significant differences in lung length or height between sides or postures. Cranial lung width decreased significantly in the flexed state (p < 0.05), while overall lung volume dropped markedly from 29.93 ± 0.33 cm³ (left) and 29.77 ± 0.33 cm³ (right) in extension to 23.41 ± 0.33 cm³ and 23.31 ± 0.32 cm³ in flexion (p < 0.001). Caudal parenchymal thickness was significantly reduced in flexed posture (p < 0.05). Coelomic cavity occupancy decreased from 43.06% ± 0.44% to 34.10% ± 0.47% (p < 0.001). These findings demonstrate that E. orbicularis lungs maintain overall shape during postural changes but undergo substantial cranial width and volume reduction with caudal parenchymal thinning. Differences between CT and casting highlight the complementary value of both techniques, with CT capturing functional, in vivo configurations and casting revealing intricate bronchial anatomy. The data provide new insights into respiratory adaptation and volumetric modulation in semi-aquatic chelonians, with relevance for comparative anatomy, evolutionary biology, and clinical imaging applications.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70125"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148018708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian Follicular Typology in Coypu (Myocastor coypus bonariensis).","authors":"Felipe Antonio Eduardo, Killian Marcelo Pablo, Lombardo Daniel Marcelo","doi":"10.1111/ahe.70131","DOIUrl":"10.1111/ahe.70131","url":null,"abstract":"<p><p>The coypu (Myocastor coypus bonariensis) is a semi-aquatic hystricomorph rodent native to South America, widely distributed across wetlands from southern Brazil to Tierra del Fuego. The general features of the ovary of the coypu older than 6 months are similar to those described for other hystricomorphs. The objective of this research was to expand the qualitative and quantitative characterization of the follicular typology of coypu at sexual maturity. We worked with 36 ovaries from females in different stages of the oestrous cycle. The follicles were analysed based on qualitative and quantitative characteristics, such as their size (mean follicular diameter and number of granulosa cells), size of each oocyte and its nucleus, presence of zona pellucida, antrum, and theca. Six follicular types were established, subdivided into 12 subtypes. Establishing this follicular typology in a South American species advances comparative studies. Additionally, it enables a deeper analysis of follicular development across different species.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70131"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147984487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colocalization of Dopamine D1 and D2 Receptors With Neuronal Nitric Oxide Synthase in the Rat Paraventricular Nucleus: A Structural Basis for Dopaminergic-Nitrergic Interaction.","authors":"Aixa R Bello, Manuel Mas, Ricardo Reyes","doi":"10.1111/ahe.70134","DOIUrl":"10.1111/ahe.70134","url":null,"abstract":"<p><p>The paraventricular nucleus (PVN) plays a central role in neuroendocrine and autonomic regulation, including male sexual behaviour. Dopaminergic and nitrergic signalling within the PVN are functionally linked, but their cellular relationship remains unclear. We examined the colocalization of dopamine D1 and D2 receptors with neuronal nitric oxide synthase (nNOS) in adult male rats using double-label immunohistochemistry. nNOS-immunoreactive neurons were widely distributed in PVN, with subsets co-expressing D1R or D2R. Approximately 45% of nNOS-positive neurons expressed dopaminergic receptors. These findings provide structural evidence for dopaminergic-nitrergic interaction in the PVN and support the possibility of direct dopaminergic modulation of nitrergic neurons.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70134"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147984513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Qualitative and Quantitative Study of Musculature Within the Antebrachial and Carpal Regions in Felis catus.","authors":"Ismail Hakki Nur, William Pérez, Catrin Sian Rutland, Horst Erich König, Nejat Unlukal, Merve Solmaz, Seda Simsek, Ender Erdogan","doi":"10.1111/ahe.70130","DOIUrl":"10.1111/ahe.70130","url":null,"abstract":"<p><p>This study was conducted to describe the anatomical features of the manus (paw) muscles in domestic cats, using five males and five females. It provides the first detailed data on the diameters of the digital sesamoid bones and describes, for the first time, the extensor, flexor, abductor and adductor muscles of the first digit, including the abductor pollicis brevis. The flexor digitorum superficialis gave five extensions to the digits and formed the manica flexoria in all but the first digit. The Mm. interossei dorsales were observed as in humans, with distinct origins and insertions. The extensor digiti I [pollicis] was found to be twice as wide as that of digit II. In most specimens, interflexor branches to digits II-IV merged with the flexor digitorum superficialis tendons at the metacarpophalangeal joint. Each manus contained four lumbrical muscles and five vincula tendinum, the latter composed of fibroelastic fibres and blood vessels. It is suggested that the term ligamenta accessorii be used for the vincula tendinum. The lumbrical muscles attached to the metacarpophalangeal joint and coursed toward the middle phalanx, joining the extensor digitorum communis tendons. Among the first digit muscles, the adductor digiti I [pollicis] was the thicker, while the flexor brevis was thicker than the abductor brevis. Histology confirmed fibrocytes and vessels within the ligg. accessorii. This represents the most detailed study of feline manus muscles to date and is expected to contribute significantly to veterinary anatomy.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70130"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148018701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Low-Cost Technique for Three-Dimensional Cardiopulmonary Preservation Using Internal Poly(Vinyl Acetate) (PVAc) Injection.","authors":"Fabio Cesar Magioli Abdala, Silvio Pires Gomes, Silmara Santos Lazzaretti, Maria Clara Ucker Costódio, Bruno Cesar Schimming, Rinaldo José Ortiz, Vinicius Pedro Silva de Oliveira, Selma Maria Michelin Matheus, Tais Harumi de Castro Sasahara","doi":"10.1111/ahe.70137","DOIUrl":"10.1111/ahe.70137","url":null,"abstract":"<p><p>Anatomical preservation is essential for teaching and research; however, conventional formaldehyde-based techniques present limitations due to toxicity, volatility and environmental impact. Plastination provides excellent morphological quality, but its high cost, reliance on flammable solvents and requirement for specialized equipment limit its accessibility. This study proposes a low-cost alternative technique for the preservation of hearts and lungs using internal application of poly(vinyl acetate) (PVAc) followed by air drying. Organs from two canines, one ovine and one lagomorph were injected with PVAc, fixed in 3.7% formaldehyde for 72 h, and subsequently dehydrated at room temperature for approximately 30 days. The resulting specimens maintained their three-dimensional conformation, exhibited reduced weight, absence of odour and good resistance to handling, and allowed subsequent painting for didactic purposes. Despite some tissue darkening, overall morphological integrity was preserved, producing specimens suitable for anatomical demonstration. This technique represents a simple, reproducible and cost-effective alternative to traditional plastination, significantly reducing formaldehyde exposure and eliminating the need for flammable solvents or complex laboratory infrastructure.</p>","PeriodicalId":50794,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 4","pages":"e70137"},"PeriodicalIF":1.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148037254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}