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Multimodal data integration using deep learning predicts overall survival of patients with glioma 利用深度学习进行多模态数据整合可预测胶质瘤患者的总体生存率

View Pub Date : 2024-08-08 DOI: 10.1002/viw.20240001

Yifan Yuan, Xuan Zhang, Yining Wang, Hongyan Li, Zengxin Qi, Zunguo Du, Ying‐Hua Chu, Danyang Feng, Jie Hu, Qingguo Xie, Jianping Song, Yuqing Liu, Jiajun Cai

{"title":"Multimodal data integration using deep learning predicts overall survival of patients with glioma","authors":"Yifan Yuan, Xuan Zhang, Yining Wang, Hongyan Li, Zengxin Qi, Zunguo Du, Ying‐Hua Chu, Danyang Feng, Jie Hu, Qingguo Xie, Jianping Song, Yuqing Liu, Jiajun Cai","doi":"10.1002/viw.20240001","DOIUrl":"https://doi.org/10.1002/viw.20240001","url":null,"abstract":"Gliomas are highly heterogenous diseases with poor prognosis. Precise survival prediction could benefit further clinical decision‐making, clinical trial incursion, and health economics. Recent research has emphasized the prognostic value of magnetic resonance imaging, pathological specimens, and circulating biomarkers. However, the integrative potential and efficacy of these modalities require to be further validated. After incorporating 218 patients of The Cancer Genome Atlas glioma datasets of and 54 patients of the Huashan cohort with complementary prognostic information, we established a squeeze‐and‐excitation deep learning feature extractor for T1‐contrast enhanced images and histological slides and explored to screen significant circulating 5‐hydroxymethylcytosines (5hmC) profiles for glioma survival by least absolute shrinkage and selection operator‐Cox regression. Therefore, a prognostication predictive model with high efficiency was obtained through survival support vector machine multimodal integration of radiologic imaging, histopathologic imaging features, genome‐wide 5hmC in circulating cell‐free DNA and clinical variables, suggesting a valid strategy (concordance‐index = 0.897; Brier score = 0.118) for improved survival risk stratification of glioma patients.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141928040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements of non‐invasive imaging technologies for the diagnosis and staging of liver fibrosis: Present and future 用于肝纤维化诊断和分期的无创成像技术的进展：现状与未来

View Pub Date : 2024-07-15 DOI: 10.1002/viw.20240010

Wenpeng Huang, Yushuo Peng, L. Kang

{"title":"Advancements of non‐invasive imaging technologies for the diagnosis and staging of liver fibrosis: Present and future","authors":"Wenpeng Huang, Yushuo Peng, L. Kang","doi":"10.1002/viw.20240010","DOIUrl":"https://doi.org/10.1002/viw.20240010","url":null,"abstract":"Liver fibrosis is a reparative response triggered by liver injury. Non‐invasive assessment and staging of liver fibrosis in patients with chronic liver disease are of paramount importance, as treatment strategies and prognoses depend significantly on the degree of fibrosis. Although liver fibrosis has traditionally been staged through invasive liver biopsy, this method is prone to sampling errors, particularly when biopsy sizes are inadequate. Consequently, there is an urgent clinical need for an alternative to biopsy, one that ensures precise, sensitive, and non‐invasive diagnosis and staging of liver fibrosis. Non‐invasive imaging assessments have assumed a pivotal role in clinical practice, enjoying growing popularity and acceptance due to their potential for diagnosing, staging, and monitoring liver fibrosis. In this comprehensive review, we first delved into the current landscape of non‐invasive imaging technologies, assessing their accuracy and the transformative impact they have had on the diagnosis and management of liver fibrosis in both clinical practice and animal models. Additionally, we provided an in‐depth exploration of recent advancements in ultrasound imaging, computed tomography imaging, magnetic resonance imaging, nuclear medicine imaging, radiomics, and artificial intelligence within the field of liver fibrosis research. We summarized the key concepts, advantages, limitations, and diagnostic performance of each technique. Finally, we discussed the challenges associated with clinical implementation and offer our perspective on advancing the field, hoping to provide alternative directions for the future research.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141647494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complexes of bacteria‐recognizing engineered phage lysin and red‐colored bacteria microparticles as optical bioprobes for simple, rapid, naked‐eye detection of syphilis‐specific antibodies from clinical samples 细菌识别工程噬菌体溶酶和红色细菌微颗粒复合物作为光学生物探针，用于从临床样本中简单、快速、裸眼检测梅毒特异性抗体

View Pub Date : 2024-06-10 DOI: 10.1002/viw.20230120

Wei Hu, Lin Zhang, Ye Tan, Jian Luo, Jingping Xin, Gen Zhang, Jinyin Xu, Yangyang Zhang, Ya Xu, Ke Li, Yan Zhang, Chuanbin Mao

{"title":"Complexes of bacteria‐recognizing engineered phage lysin and red‐colored bacteria microparticles as optical bioprobes for simple, rapid, naked‐eye detection of syphilis‐specific antibodies from clinical samples","authors":"Wei Hu, Lin Zhang, Ye Tan, Jian Luo, Jingping Xin, Gen Zhang, Jinyin Xu, Yangyang Zhang, Ya Xu, Ke Li, Yan Zhang, Chuanbin Mao","doi":"10.1002/viw.20230120","DOIUrl":"https://doi.org/10.1002/viw.20230120","url":null,"abstract":"Serological detection of syphilis‐specific antibodies is the most widely used clinical method for diagnosing syphilis. However, the current methods in clinical practice are too diverse, complicating result interpretation and wasting medical resources. To meet the resultant demand for a simple fast naked‐eye detection method, we employed the bacterial cell‐binding domain (CBD) of bacteriophage lysin to functionalize bio‐microparticles (engineered non‐culturable red‐colored Staphylococcus aureus bacteria), forming a bioprobe that can detect the syphilis‐specific antibodies from human serum in 5 min with naked eyes. Specifically, CBD is bioengineered by fusion with one of three antigens of the syphilis‐causing bacteria Treponema pallidum separately. The three resulting fusion proteins bind to the bacteria surface through the CBD motif, generating a bioprobe with the antigens exposed. When the bioprobe is added to the serum samples collected from the syphilis patients, the syphilis‐specific antibodies bind the antigens on the bioprobes and cross‐link them, forming eye‐visible red aggregates for the naked‐eye antibody detection. The bioprobe was validated on 209 clinical samples, revealing its high clinical sensitivity and specificity. Exploiting the natural biorecognition between the bacteria‐specific phage‐derived species and the phage host bacteria represents a promising strategy for producing facile probes for rapid point‐of‐care testing of infectious diseases.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel strategies for enhanced fluorescence visualization of glioblastoma tumors based on HPMA copolymers conjugated with tumor targeting and/or cell‐penetrating peptides 基于与肿瘤靶向肽和/或细胞穿透肽共轭的 HPMA 共聚物的胶质母细胞瘤肿瘤荧光可视化增强新策略

View Pub Date : 2024-04-24 DOI: 10.1002/viw.20230116

Eliška Grosmanová, R. Pola, M. Filipová, M. Henry, Jean-Luc Coll, T. Etrych

{"title":"Novel strategies for enhanced fluorescence visualization of glioblastoma tumors based on HPMA copolymers conjugated with tumor targeting and/or cell‐penetrating peptides","authors":"Eliška Grosmanová, R. Pola, M. Filipová, M. Henry, Jean-Luc Coll, T. Etrych","doi":"10.1002/viw.20230116","DOIUrl":"https://doi.org/10.1002/viw.20230116","url":null,"abstract":"Nano‐sized polymer systems are often used as carriers for drugs and contrast agents to increase circulation time and solubility and to reduce possible side effects. These nanomedicines usually accumulate in tumor tissue due to the enhanced permeability and retention (EPR) effect. However, a targeting group may be attached to the polymer carrier in addition to the active substance to further increase tumor accumulation and specificity. In this study, the oligopeptide sequence RGD was chosen to target αvβ3 integrins overexpressed in the tumor vasculature and on some tumor cells. A set of polymer conjugates bearing a fluorescent dye and RGD peptide of different structures (linear, cyclic, branched) was prepared for use in tumor diagnosis, with a potential future application in navigated surgery. The accumulation of the most promising candidate, a targeted fluorescent nanoprobe, increased by 35% in glioblastoma tumors compared to the non‐targeted control, which accumulated only due to the EPR effect. However, the administration of a polymer‐bound modified cilengitide as an antiangiogenic treatment did not show a beneficial effect in the suppression of angiogenesis.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140664107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanomedicine‐encouraged cellular autophagy promoters favor liver fibrosis progression reversal 纳米药物鼓励的细胞自噬促进因子有利于逆转肝纤维化进程

View Pub Date : 2024-04-23 DOI: 10.1002/viw.20240003

Cheng Qian, Yan Zhang, Xiaoyang Chen, Chunyan Zhu, Xiulin Dong, Weiwei Chen, Xuejun Ni, Kun Zhang, Yifei Yin

{"title":"Nanomedicine‐encouraged cellular autophagy promoters favor liver fibrosis progression reversal","authors":"Cheng Qian, Yan Zhang, Xiaoyang Chen, Chunyan Zhu, Xiulin Dong, Weiwei Chen, Xuejun Ni, Kun Zhang, Yifei Yin","doi":"10.1002/viw.20240003","DOIUrl":"https://doi.org/10.1002/viw.20240003","url":null,"abstract":"Liver fibrosis is a major risk factor for hepatocellular carcinoma origin, and its progression not only correlates with oxidative stress and inflammation, but also is encouraged by autophagy hold‐up. Therefore, new solutions to effectively attenuate oxidative stress and inflammation and coincidently favor autophagy are highly demanded to reverse liver fibrosis, and even hamper its escalation into hepatocellular carcinoma. Herein, the porous manganese‐substituted Prussian blue (PMPB) analogs are harnessed to activate autophagy, scavenge reactive oxygen species (ROS), and suppress inflammation for liver fibrosis therapy. PMPB can effectively inhibit macrophage activation, facilitate macrophage autophagy, eradicate ROS, and blockade cellular cross‐talk, thus impeding further inflammation progression. Moreover, the favorable spontaneous capture of PMPB by Kupffer cells allows more PMPB accumulation in liver to significantly attenuate liver injury and collagen deposition, thereby inhibiting the progression of liver fibrosis. PMPB‐based nanomedicine shows great potentials in promoting autophagy activation, eliminating ROS, inhibiting inflammation, and protecting hepatocytes from oxidative stress‐arised damages, which eventually attenuate the extent of liver fibrosis, holding great promise in clinical translation for treating liver fibrosis.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140669060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface‐enhanced Raman scattering spatial fingerprinting decodes the digestion behavior of lysosomes in live single cells 表面增强拉曼散射空间指纹解码活体单细胞中溶酶体的消化行为

View Pub Date : 2024-04-19 DOI: 10.1002/viw.20240004

Fugang Liu, Zhirui Sun, Bingyi Li, Jiaqing Liu, Zhou Chen, Jian Ye

{"title":"Surface‐enhanced Raman scattering spatial fingerprinting decodes the digestion behavior of lysosomes in live single cells","authors":"Fugang Liu, Zhirui Sun, Bingyi Li, Jiaqing Liu, Zhou Chen, Jian Ye","doi":"10.1002/viw.20240004","DOIUrl":"https://doi.org/10.1002/viw.20240004","url":null,"abstract":"Lysosome, the digestive organelle in eukaryotic cells, plays an important role in the degradation and recirculation of cellular products as well as in maintaining the stability of cellular metabolic microenvironment. Surface‐enhanced Raman scattering (SERS) is a molecular fingerprint technology with high detection sensitivity and photostability, suited for revealing various intracellular molecular information by inducing endocytosis of SERS‐active nanoparticles. However, it remains challenging to selectively extract the molecular information of specific organelles (e.g., lysosomes) from a high‐dimensional spectral set. Herein, we proposed a novel paradigm by combining label‐free SERS spectroscopy with confocal fluorescence imaging to investigate the digestion behavior of lysosomes in cells. The structural similarity algorithm was innovatively introduced and exhibited its effectiveness in screening out the wavenumbers in the SERS spectral set with high correlation with the metabolic behaviors of lysosomes. With comprehensive experiments on HeLa single cells, we captured the intracellular macromolecular digestion phenomenon and discovered the changing pattern of cellular SERS spectra after starvation‐induced autophagy, and analyzed the molecular information within the lysosomes in three‐dimensional space.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140682796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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View Pub Date : 2024-04-01 DOI: 10.1002/viw2.291

引用次数: 0

The study of anti‐rabies virus effect of Shougong powder 首乌粉的抗狂犬病毒作用研究

View Pub Date : 2024-02-05 DOI: 10.1002/viw.20230033

Minmin Zhang, Yun Fan, Xueliang Wang, Jinghua Wang, Rong Chen, Husheng Pan, Yanqun Xiao, Heyao Wang, Weigang Huang, Hualiang Wang

{"title":"The study of anti‐rabies virus effect of Shougong powder","authors":"Minmin Zhang, Yun Fan, Xueliang Wang, Jinghua Wang, Rong Chen, Husheng Pan, Yanqun Xiao, Heyao Wang, Weigang Huang, Hualiang Wang","doi":"10.1002/viw.20230033","DOIUrl":"https://doi.org/10.1002/viw.20230033","url":null,"abstract":"Rabies is a lethal infectious disease caused by rabies virus (RABV). The mortality rate is very high after the appearance of clinical symptoms, with a survival rate of almost 0%. There is presently no cure for rabies. In the present study, we investigated whether the extract of Shougong powder—a calcium powder prepared from gecko that has demonstrated immunomodulatory properties in mice—is an effective treatment for rabies. The antiviral effects of the extract were evaluated both in vitro and in vivo with the cytotoxicity and antiviral assays and by immunofluorescence analysis, quantitative real‐time (qRT)‐PCR, and western blotting. The results showed that Shougong powder and its extract increased survival rate in RABV‐infected mice is up to 60% and 50%, respectively, even in 20 times of LD50, whereas the control groups treated with isoprinosine (IPS) or saline are only 20% and 0% survival (p = .011). qRT‐PCR and western blotting analyses showed that the extract strongly inhibited viral mRNA expression and protein synthesis in vitro: expression of the N, P, M, G, and L genes of RABV was decreased by 28.8%–45.0% in the IPS group (p < .05) and by 50.1%–59.0% in the extract group (p < .05) relative to the control group. These results demonstrate that Shougong powder has certain antiviral effects against RABV and can potentially be used for the treatment for rabies.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139803888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The study of anti‐rabies virus effect of Shougong powder 首乌粉的抗狂犬病毒作用研究

View Pub Date : 2024-02-05 DOI: 10.1002/viw.20230033

Minmin Zhang, Yun Fan, Xueliang Wang, Jinghua Wang, Rong Chen, Husheng Pan, Yanqun Xiao, Heyao Wang, Weigang Huang, Hualiang Wang

{"title":"The study of anti‐rabies virus effect of Shougong powder","authors":"Minmin Zhang, Yun Fan, Xueliang Wang, Jinghua Wang, Rong Chen, Husheng Pan, Yanqun Xiao, Heyao Wang, Weigang Huang, Hualiang Wang","doi":"10.1002/viw.20230033","DOIUrl":"https://doi.org/10.1002/viw.20230033","url":null,"abstract":"Rabies is a lethal infectious disease caused by rabies virus (RABV). The mortality rate is very high after the appearance of clinical symptoms, with a survival rate of almost 0%. There is presently no cure for rabies. In the present study, we investigated whether the extract of Shougong powder—a calcium powder prepared from gecko that has demonstrated immunomodulatory properties in mice—is an effective treatment for rabies. The antiviral effects of the extract were evaluated both in vitro and in vivo with the cytotoxicity and antiviral assays and by immunofluorescence analysis, quantitative real‐time (qRT)‐PCR, and western blotting. The results showed that Shougong powder and its extract increased survival rate in RABV‐infected mice is up to 60% and 50%, respectively, even in 20 times of LD50, whereas the control groups treated with isoprinosine (IPS) or saline are only 20% and 0% survival (p = .011). qRT‐PCR and western blotting analyses showed that the extract strongly inhibited viral mRNA expression and protein synthesis in vitro: expression of the N, P, M, G, and L genes of RABV was decreased by 28.8%–45.0% in the IPS group (p < .05) and by 50.1%–59.0% in the extract group (p < .05) relative to the control group. These results demonstrate that Shougong powder has certain antiviral effects against RABV and can potentially be used for the treatment for rabies.","PeriodicalId":507490,"journal":{"name":"View","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139863452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: (View 1/2024) 刊头：（查看 1/2024）

View Pub Date : 2024-02-01 DOI: 10.1002/viw2.290

引用次数: 0