Antibiotiques最新文献

Technology advancements in antibody purification 抗体纯化技术进展

Antibiotiques Pub Date : 2016-08-26 DOI: 10.2147/ANTI.S64762

C. Murphy, T. Devine, R. O’Kennedy

引用次数: 23

Broadly neutralizing antibodies for therapy of viral infections 广泛中和抗体用于治疗病毒感染

Antibiotiques Pub Date : 2016-01-08 DOI: 10.2147/ANTI.S92190

Sankaranarayanan Srinivasan, Maloy Ghosh, Sunit Maity, Raghavan Varadarajan

引用次数: 2

Antibody-based arrays in disease proteomics 疾病蛋白质组学中基于抗体的阵列

Antibiotiques Pub Date : 2015-02-09 DOI: 10.2147/ANTI.S53335

Daniel A Guthy, Hans Voshol

引用次数: 1

Advances in the development of antibody-based immunotherapy against prion disease 朊病毒疾病的抗体免疫治疗进展

Antibiotiques Pub Date : 2014-08-30 DOI: 10.2147/ANTI.S53336

Huiying Gu, R. Dodel, M. Farlow, Yansheng Du

{"title":"Advances in the development of antibody-based immunotherapy against prion disease","authors":"Huiying Gu, R. Dodel, M. Farlow, Yansheng Du","doi":"10.2147/ANTI.S53336","DOIUrl":"https://doi.org/10.2147/ANTI.S53336","url":null,"abstract":"Les integrons sont des structures genetiques lineaires d'ADN qui servent a la capture de genes et de vecteurs d'expression. La structure basique d'un integron inclut : une enzyme integrase appartenant a la famille des recombinases specifiques de type, un promoteur, et un site d'insertion attI, auquel de l'ADN additionnel sous forme de cassettes de genes est insere par l'action de l'integrase. Les integrons de Classe I sont les plus communs et consistent normalement en 2 segments conserves d'ADN localise de chaque cote des cassettes de genes, la plupart correspondant a des genes de resistance aux antibiotiques. Ces cassettes de genes sont capables d'insertion a mediation integrase ou de deletion entre les segments conserves dans differents ordres et combinaisons. Les integrons agissent comme vecteurs en apportant un promoteur pour la transcription (expression) des cassettes de genes integres. Plus de 60 differentes cassettes de genes ont ete decrites, conferant la resistance vis-a-vis de nombreuses familles d'antibiotiques utilises contre les bacteries a Gram negatif, ces integrons etant trouves chez les souches cliniques de Gram negatifs dans le monde entier. Ils sont associes plus particulierement a la resistance aux aminoglycosides des souches d'origine clinique. L'emergence recente d'integrons hebergeant la resistance aux carbapenemes constitue un developpement reellement menacant. Il apparait clairement que les integrons permettent aux bacteries une adaptation continue et rapide a l'emploi en clinique de nouveaux antibiotiques, par l'acquisition de nouvelles cassettes de genes de resistance. En plus de leur role dans la dissemination de resistance aux antibiotiques, la mise en evidence de structures dites « super-integron » suggere que les integrons fonctionnent en tant que systemes de « gene-capture » plus generaux pour l'adaptation et l'evolution des bacteries.","PeriodicalId":50747,"journal":{"name":"Antibiotiques","volume":"4 1","pages":"45-55"},"PeriodicalIF":0.0,"publicationDate":"2014-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/ANTI.S53336","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68302220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Antibody-based techniques for detection of Lyme disease: a challenging issue 基于抗体的莱姆病检测技术:一个具有挑战性的问题

Antibiotiques Pub Date : 2014-07-30 DOI: 10.2147/ANTI.S39718

J. Zajkowska

{"title":"Antibody-based techniques for detection of Lyme disease: a challenging issue","authors":"J. Zajkowska","doi":"10.2147/ANTI.S39718","DOIUrl":"https://doi.org/10.2147/ANTI.S39718","url":null,"abstract":"Serologic assays detecting antibodies to Borrelia burgdorferi are useful in the routine diagnosis of Lyme disease. Despite the presence of new and improved assays, current laboratory diagnosis still requires optimization. The variety of genospecies and their different geographic distributions are the reasons why standards and recommendations are not the same for all of the main geographic regions, ie, USA, Asia, and Europe. Moreover, the variety and variability of antigens represents a significant challenge in assay design. This is due to the various antigens among the genospecies responsible for Lyme borreliosis; the changing antigens presented during infection; and the variability of single antigens. This review article discusses the immunologic response in Lyme disease over time, and the advantages and disadvantages of existing serological tests. Two tier testing is also introduced. Antigens useful for diagnosis, the properties of individual antigens and their appearance in infection especially the antigens appearing during mammalian infection, so-called “in vivo” antigens are introduced. To determine antibodies confirming infection in the nervous system, the same restrictions with regard to interpretation of enzyme-linked immunosorbent assay results in serum apply to cerebrospinal fluid. Furthermore, concentrations of antibodies in the two compartments, ie, blood and cerebrospinal fluid, are variable depending on compartmentalization (anatomic sequestration) and immunologic phenomena at immunologically privileged sites, such as the intrathecal space. To confirm neuroborreliosis, synthesis of antibodies in cerebrospinal fluid, should be measured in the form of a so-called antibody index. Further studies should focus on detecting the lowest concentration of antibodies and looking for useful new antigens, and the relationship between composition of such antigens and the patient’s clinical status.","PeriodicalId":50747,"journal":{"name":"Antibiotiques","volume":"126 1","pages":"33-44"},"PeriodicalIF":0.0,"publicationDate":"2014-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/ANTI.S39718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68302271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

rapid detection of different human anti-hcV immunoglobulins on electrical biochips 在电子生物芯片上快速检测不同的人抗丙肝病毒免疫球蛋白

Antibiotiques Pub Date : 2014-06-26 DOI: 10.2147/ANTI.S54763

L. Blohm, C. Püttmann, Simone Holz, G. Piechotta, J. Albers, C. Dammers, M. Kleines, A. Krüttgen, G. Melmer, J. Nähring, S. Barth, E. Nebling

{"title":"rapid detection of different human anti-hcV immunoglobulins on electrical biochips","authors":"L. Blohm, C. Püttmann, Simone Holz, G. Piechotta, J. Albers, C. Dammers, M. Kleines, A. Krüttgen, G. Melmer, J. Nähring, S. Barth, E. Nebling","doi":"10.2147/ANTI.S54763","DOIUrl":"https://doi.org/10.2147/ANTI.S54763","url":null,"abstract":"The detection of hepatitis C virus (HCV) in the blood of patients is currently based on immunological assays (enzyme-linked immunosorbent assay (ELISA) and recombinant immunoblot assay) that use different HCV epitopes to detect anti-HCV antibodies, and these tests usually require laboratories and trained personnel. The ELISA-based systems are also time consuming. Portable diagnostic devices offering rapid test results would therefore be advanta- geous in the field of medical care. To facilitate the fast and reliable diagnosis of HCV, we used a miniaturized automated system based on a cartridge with an integrated electrical biochip for the decentralized detection of anti-HCV antibodies against the Core, NS3, and NS4A proteins. This system allows the detection of virus-specific antibodies in 2 µL of serum or whole blood within 15 minutes using an ELISA directly on a gold electrode array containing HCV proteins as the capture antigen. The sensitivity of this system is comparable with standard microtiter plate ELISAs, but the duration of the novel assay is 5%-6% that of standard ELISAs.","PeriodicalId":50747,"journal":{"name":"Antibiotiques","volume":"4 1","pages":"23-32"},"PeriodicalIF":0.0,"publicationDate":"2014-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/ANTI.S54763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68302687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Pathogen-specific antigen target for production of antibodies produced by comparative genomics 病原体特异性抗原靶标，用于比较基因组学产生的抗体的生产

Antibiotiques Pub Date : 2014-05-09 DOI: 10.2147/ANTI.S54848

A. Lathrop, Taylor W. Bailey, Kwang-Pyo Kim, A. Bhunia

{"title":"Pathogen-specific antigen target for production of antibodies produced by comparative genomics","authors":"A. Lathrop, Taylor W. Bailey, Kwang-Pyo Kim, A. Bhunia","doi":"10.2147/ANTI.S54848","DOIUrl":"https://doi.org/10.2147/ANTI.S54848","url":null,"abstract":": Listeria monocytogenes continues to be a major public health risk and there is a need for improved rapid detection methods. New and highly specific L. monocytogenes antibodies are needed to advance current detection and meet the needs of industry. This research compared the L. monocytogenes genome with that of L. innocua (a nonpathogenic species of Listeria ) and identified nine surface proteins specific to L. monocytogenes . Protein sequences were collected from the database and properties such as hydropathy profile and transmembrane topology were analyzed using TMpred software. Nine peptide sequences were chosen, and synthetic peptides were made and administered to rabbits for antibody production. All nine antibodies were screened against a panel of L. monocytogenes , nonpathogenic Listeria , and non-Listeria bacteria. Two of the nine antibodies, ie, the Lm404 and LmC639 polyclonal antibodies, showed a specific reaction to L. monocytogenes internalin B and actin polymerization protein, respectively, and were characterized further by enzyme-linked immunosorbent assay, Western blot, and transmission electron microscopy. In Western blot, both antibodies reacted with the targeted protein and did not cross-react with other Listeria spp. The Lm404 polyclonal antibody showed a high reaction with the panel of 41 L. monocytogenes strains while the LmC639 polyclonal antibody showed a weak reaction. Both the Lm404 and LmC639 polyclonal antibodies showed potential for use in immunoassays for specific detection of L. monocytogenes . This study further indicates that comparative genomics could be used to select pathogen-specific antigen for antibody production.","PeriodicalId":50747,"journal":{"name":"Antibiotiques","volume":"4 1","pages":"13-22"},"PeriodicalIF":0.0,"publicationDate":"2014-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2147/ANTI.S54848","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68302838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Monoclonal antibodies for the prevention of rabies: theory and clinical practice 预防狂犬病的单克隆抗体:理论与临床应用

Antibiotiques Pub Date : 2014-01-20 DOI: 10.2147/ANTI.S33533

T. Nagarajan, W. Marissen, Charles E. Rupprecht

引用次数: 11

Human hybridoma technology 人类杂交瘤技术

Antibiotiques Pub Date : 2012-08-31 DOI: 10.2147/ANTI.S30489

M. Gorny

引用次数: 11

Detection of in situ cleaved p115 with the cut specific antibodies in rapid protein inactivation system by tobacco etch viral protease cleavage 烟草刻蚀病毒蛋白酶快速蛋白失活系统中原位裂解p115的特异性抗体检测

Antibiotiques Pub Date : 2011-08-10 DOI: 10.2147/ANTI.S22825

Mayuko Koreishi, Yasuko Honjo, Ayano Satoh

引用次数: 1