Fermentation最新文献_第2页

Gut-Derived Lactic Acid Bacteria from Cotton Bollworm Exhibit Efficient Gossypol Degradation and Probiotic Potential During Solid-State Fermentation of Cottonseed Meal 棉铃虫肠道来源的乳酸菌在棉籽粕固态发酵过程中表现出高效的棉酚降解和益生菌潜力

Fermentation Pub Date : 2025-10-19 DOI: 10.3390/fermentation11100598

Sijin Li, Shangya Deng, Peng Zhang, Qicheng Lu, Wei Pu, Mingyu Ma, Shu Li, Wenju Zhang, Cheng Chen

{"title":"Gut-Derived Lactic Acid Bacteria from Cotton Bollworm Exhibit Efficient Gossypol Degradation and Probiotic Potential During Solid-State Fermentation of Cottonseed Meal","authors":"Sijin Li, Shangya Deng, Peng Zhang, Qicheng Lu, Wei Pu, Mingyu Ma, Shu Li, Wenju Zhang, Cheng Chen","doi":"10.3390/fermentation11100598","DOIUrl":"https://doi.org/10.3390/fermentation11100598","url":null,"abstract":"Cottonseed meal (CSM), an important protein-rich feed ingredient, faces limited utilization in livestock diets due to the presence of free gossypol (FG)—a potent antinutritional toxin. This study aimed to isolate FG-degrading bacteria from the cotton bollworm, Helicoverpa armigera, and to evaluate their potential as probiotics in vitro. Eleven gossypol-tolerant strains were isolated from the gut of Helicoverpa armigera larvae using a screening medium containing gossypol as the sole carbon source. Among these, four lactic acid bacteria strains—Pediococcus acidilactici GM-NP, Pediococcus acidilactici GM-P, Enterococcus faecalis GM-6, and Weissella confusa GM-2—were selected for further investigation of their gossypol degradation capacity and probiotic potential. Probiotic characterization revealed that all strains exhibited tolerance to gastrointestinal fluids and bile salts, safe γ-hemolysis, and strong auto-aggregation, cell surface hydrophobicity, and antimicrobial activity. Solid-state fermentation of CSM with these strains reduced FG content by more than 50%, increased crude protein by over 6%, and elevated acid-soluble protein content by more than 70%, thereby effectively enhancing the nutritional quality of CSM. This study is the first to demonstrate that bacterial isolates from the gut of Helicoverpa armigera possess concurrent high-efficiency gossypol degradation and probiotic properties, providing a theoretical foundation for developing novel probiotic resources and promoting the safe utilization of CSM.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 10","pages":"598-598"},"PeriodicalIF":0.0,"publicationDate":"2025-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/10/598/pdf?version=1760852937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Transforming Tilapia into Indoleacetic Acid-Containing Biostimulants: Synergistic Effect of Enzymolysis and Multi-Strain Fermentation 罗非鱼转化为含吲哚乙酸生物刺激素：酶解和多菌种发酵的协同效应

Fermentation Pub Date : 2025-09-08 DOI: 10.3390/fermentation11090525

H.-L. Xie, Bin Zhong, Qimin Zhang, Xi Hu, Xuesen Xia, Xie Hong, Zhenqiang Wu

{"title":"Transforming Tilapia into Indoleacetic Acid-Containing Biostimulants: Synergistic Effect of Enzymolysis and Multi-Strain Fermentation","authors":"H.-L. Xie, Bin Zhong, Qimin Zhang, Xi Hu, Xuesen Xia, Xie Hong, Zhenqiang Wu","doi":"10.3390/fermentation11090525","DOIUrl":"https://doi.org/10.3390/fermentation11090525","url":null,"abstract":"Following new trends in green development, many studies have focused on the high-value utilization of fish resources through green biological processes. This study innovatively introduced a one-step process of mixed strain–enzyme synergy (MES) with which to prepare tilapia hydrolysates and explored the synergistic effects of strains and enzymes on both the protein hydrolysis process and its products’ characteristics via comparative experiments. Further, soybean was used as a model crop to verify the agronomic effects of the hydrolysates. The addition of exogenous papain increased hydrolysis by 31.94% compared to the fermentation-only group. Peptides and amino acids contents in the mixed strains were higher than those in the single fermentation process (p < 0.05), while 8.46 mg/L of indoleacetic acid was produced through fermentation. Hydrolysates promoted the growth of lateral roots in soybean seedlings (p < 0.05) via the use of a 2500-fold dilution of the biostimulant, increasing the root area and stem length and reducing the sugar content of soybean seedlings by 1.59-, 1.44- and 1.69-fold compared to those in Hoagland’s nutrient solution. These results lay a foundation for the biological preparation of biostimulants for hydroponic vegetables through the utilization of fish waste resources, aligning with green development goals.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 9","pages":"525-525"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/9/525/pdf?version=1757332225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Monascus purpureus Culture Conditions in Rice Bran for Enhanced Monascus Pigment Biosynthesis 米糠中红曲霉培养条件优化促进红曲霉色素生物合成

Fermentation Pub Date : 2025-08-28 DOI: 10.3390/fermentation11090505

Di Chen, Yanping Xu, Han Li, Xuemin Zhu

{"title":"Optimization of Monascus purpureus Culture Conditions in Rice Bran for Enhanced Monascus Pigment Biosynthesis","authors":"Di Chen, Yanping Xu, Han Li, Xuemin Zhu","doi":"10.3390/fermentation11090505","DOIUrl":"https://doi.org/10.3390/fermentation11090505","url":null,"abstract":"Monascus pigments (MPs) are the most valuable secondary metabolites of Monascus. To improve the production of MPs is of great importance to food processing. Currently, studies using rice bran as the substrate to produce MPs are rare. In this study, rice bran with different carbon sources and cellulase hydrolysis conditions were explored in Monascus purpureus M9 in this study. Through single-factor experiments and Box–Behnken response surface optimization, we demonstrated that mannitol supplementation combined with cellulase treatment of substrate significantly enhanced the yields of MPs. The optimal conditions (4.00% mannitol, cellulase hydrolysis at 60 °C for 2 h) achieved a maximum color value of 3538 U/g. Furthermore, comparative evaluation under different culture conditions, including only rice bran (RB), cellulase hydrolysis of rice bran (Cel), rice bran supplemented with mannitol (Man), mannitol supplementation combined with cellulase pretreatment of substrate (Opti), and only rice (Rice), confirmed the effectiveness of the optimized treatment. The color value of the Opti group was 27.95 times more than that of the RB group and reached 80.96% of the counterpart of the Rice group. The Opti group also significantly enhanced the yields of two orange pigments (Monascorubrin and Rubropunctatin), induced more sexual spore formation, and exhibited the maximum biomass and colony diameter among different groups. The hyphae of the Man and Opti groups were full, intact, and tubular. The citrinin content in the Opti group was under the limit standard of China. The data provides a theoretical basis reference for improving the yields of MPs with RB as the substrate.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 9","pages":"505-505"},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/9/505/pdf?version=1756374150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Isolation and Characterization of Bacteriocin-like-Producing Companilactobacillus farciminis YLR-1 and the Inhibitory Activity of Bacteriocin Against Staphylococcus aureus 产细菌素样样法氏大芽胞杆菌YLR-1的分离鉴定及细菌素对金黄色葡萄球菌的抑制活性

Fermentation Pub Date : 2025-08-11 DOI: 10.3390/fermentation11080460

Lirong Yang, Hui Su, Jian Wang, Sijia Sun, Sibo Liu, Baishuang Yin, Wenlong Dong, Guojiang Li

{"title":"Isolation and Characterization of Bacteriocin-like-Producing Companilactobacillus farciminis YLR-1 and the Inhibitory Activity of Bacteriocin Against Staphylococcus aureus","authors":"Lirong Yang, Hui Su, Jian Wang, Sijia Sun, Sibo Liu, Baishuang Yin, Wenlong Dong, Guojiang Li","doi":"10.3390/fermentation11080460","DOIUrl":"https://doi.org/10.3390/fermentation11080460","url":null,"abstract":"This study aimed to identify a probiotic bacterium with antagonistic activity against the foodborne pathogen Staphylococcus aureus (S. aureus) and investigate the mechanism of its antibacterial components. Growth kinetics were analyzed to assess bacterial proliferation. Acid and bile salt tolerance are vital indicators for evaluating probiotic survival in the gastrointestinal tract. The results indicated that Companilactobacillus farciminis (C. farciminis) YLR-1 not only had high tolerance to salt conditions (0.03%, 0.3%, and 0.5%) but also has a high survival rate at pH 3–4. The bacteriocin-like inhibitory substance (BLIS) isolated from C. farciminis YLR-1 was dialyzed using a membrane with a molecular weight cut-off (MWCO) of 500 Da, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The results indicate that the BLIS produced by C. farciminis YLR-1 is a small-molecule peptide. BLIS displayed pH tolerance within acidic and neutral environments (4–8) and exhibited thermostability. When treated with proteinase K, the antibacterial action of BLIS was found to be inactivated. Membrane disruption mechanisms were examined using fluorescence imaging and scanning electron microscopy (SEM). SEM and fluorescence imaging revealed that BLIS-induced membrane damage in S. aureus ATCC 25923 causes cytoplasmic leakage and cell death.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 8","pages":"460-460"},"PeriodicalIF":0.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/8/460/pdf?version=1754904952","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transcriptomic Analysis Reveals Opposing Roles of CEL1B in Sophorose- and Lactose-Induced Cellulase Expression in Trichoderma reesei Rut C30 转录组学分析揭示CEL1B在苦参和乳糖诱导的里氏木霉C30纤维素酶表达中的相反作用

Fermentation Pub Date : 2025-07-31 DOI: 10.3390/fermentation11080439

Lu Wang, Junping Fan, Xiao He, Jian Cheng, Xinyan Zhang, Tian Tian, Yonghao Li

{"title":"Transcriptomic Analysis Reveals Opposing Roles of CEL1B in Sophorose- and Lactose-Induced Cellulase Expression in Trichoderma reesei Rut C30","authors":"Lu Wang, Junping Fan, Xiao He, Jian Cheng, Xinyan Zhang, Tian Tian, Yonghao Li","doi":"10.3390/fermentation11080439","DOIUrl":"https://doi.org/10.3390/fermentation11080439","url":null,"abstract":"The β-glucosidase CEL1B has been linked to regulating cellulase expression in Trichoderma reesei, yet its inducer-specific functions and broader regulatory roles remain poorly characterized. In this study, CRISPR-Cas9-mediated gene knockout was applied in the industrial high-producing T. reesei Rut C30 to investigate CEL1B function without the confounding effects of KU70 deletion. Unlike previous studies focused solely on cellulose or lactose induction, transcriptomic analysis of the CEL1B knockout strain revealed its regulatory roles under both lactose- and sophorose-rich conditions, with sophorose representing the most potent natural inducer of cellulase expression. Under lactose induction, CEL1B deletion resulted in a 52.4% increase in cellulase activity (p < 0.05), accompanied by transcriptome-wide upregulation of β-glucosidase genes (CEL3A: 729%, CEL3D: 666.8%, CEL3C: 110.9%), cellulose-sensing receptors (CRT1: 203.0%, CRT2: 105.8%), and key transcription factors (XYR1: 2.7-fold, ACE3: 2.8-fold, VIB1: 2.1-fold). Expression of ER proteostasis genes was significantly upregulated (BIP1: 3.3-fold, HSP70: 6.2-fold), contributing to enhanced enzyme secretion. Conversely, under sophorose induction, CEL1B deletion reduced cellulase activity by 25.7% (p < 0.05), which was associated with transcriptome profiling showing significant downregulation of β-glucosidase CEL3H (66.6%) and cellodextrin transporters (TrireC30_91594: 79.3%, TrireC30_127980: 76.3%), leading to reduced cellobiohydrolase expression (CEL7A: 57.8%, CEL6A: 67.8%). This first transcriptomic characterization of the CEL1B knockout strain reveals its dual opposing roles in modulating cellulase expression in response to lactose versus sophorose, providing new strategies for optimizing inducer-specific enzyme production in T. reesei.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 8","pages":"439-439"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/8/439/pdf?version=1753951373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assisted Isolation of Camelliagenin B from Camellia oliefera Seed Cake Meal and Microbial Transformation by Bacillus subtilis ATCC 6633, Bacillus megaterium CGMCC 1.1741, and Streptomyces gresius ATCC 13273 油茶籽饼粕中茶花素B的辅助分离及枯草芽孢杆菌ATCC 6633、巨芽孢杆菌CGMCC 1.1741和绿链霉菌ATCC 13273的转化

Fermentation Pub Date : 2025-07-15 DOI: 10.3390/fermentation11070407

Richa Raj, Jingling Zhang, Jingling Zhang, Yanyan Meng, Xuewa Jiang, Wei Wang, Jian Zhang, Jian Zhang, Boyang Yu

{"title":"Assisted Isolation of Camelliagenin B from Camellia oliefera Seed Cake Meal and Microbial Transformation by Bacillus subtilis ATCC 6633, Bacillus megaterium CGMCC 1.1741, and Streptomyces gresius ATCC 13273","authors":"Richa Raj, Jingling Zhang, Jingling Zhang, Yanyan Meng, Xuewa Jiang, Wei Wang, Jian Zhang, Jian Zhang, Boyang Yu","doi":"10.3390/fermentation11070407","DOIUrl":"https://doi.org/10.3390/fermentation11070407","url":null,"abstract":"This study investigates the potential for the microbial transformation of camelliagenin B, a saponin derived from Camellia oleifera seed cake meal, to develop novel metabolites. We employed three microbial strains, specifically Bacillus subtilis ATCC 6633, Bacillus megaterium CGMCC 1.1741, and Streptomyces griseus ATCC 13273, to biotransform camelliagenin B into its derivatives. The compounds were purified and separated using chromatographic techniques, such as high-performance liquid chromatography (HPLC). Structural identification was carried out using spectroscopic methods, including nuclear magnetic resonance (NMR) and mass spectrometry (MS). Ten bioactive compounds were obtained (1a-1j), of which nine were novel with multiple tailoring reactions, such as allyl oxidation, C-C double-bond rearrangement, hydroxylation, dehydrogenation, and glycosylation, observed in camelliagenin B analogs. The structures of these compounds were determined by 1D/2D NMR and HR-ESI-MS analysis. Therefore, this study showcases the capacity of microbial transformation as a sustainable and environmentally friendly method for generating bioactive compounds from C. oleifera seed cake meals. The individual chemicals can potentially facilitate the design of novel medicinal agents, functional foods, and natural preservatives.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 7","pages":"407-407"},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/7/407/pdf?version=1752588918","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Metabolic Engineering of Escherichia coli for De Novo Biosynthesis of Mandelic Acid 新合成扁桃酸的大肠杆菌代谢工程研究

Fermentation Pub Date : 2025-06-09 DOI: 10.3390/fermentation11060331

Chang Yu Liu, Xuefeng Xiao, Wenzhong Xing, Renuka Na, Y. J. Song, Guoqiang Cao, Pengchao Wang

{"title":"Metabolic Engineering of Escherichia coli for De Novo Biosynthesis of Mandelic Acid","authors":"Chang Yu Liu, Xuefeng Xiao, Wenzhong Xing, Renuka Na, Y. J. Song, Guoqiang Cao, Pengchao Wang","doi":"10.3390/fermentation11060331","DOIUrl":"https://doi.org/10.3390/fermentation11060331","url":null,"abstract":"Mandelic acid (MA) is a valuable α-hydroxy acid with applications in pharmaceuticals, cosmetics, and fine chemicals. While chemical synthesis is well established, concerns over toxicity and sustainability have driven interest in microbial production. Here, we engineered Escherichia coli for de novo MA biosynthesis by integrating enzyme screening, metabolic flux optimization, and pathway regulation. We first screened and identified an efficient hydroxymandelate synthase (HMAS) homolog from Actinosynnema mirum for MA synthesis, and subsequently enhanced the shikimate pathway along with the supply of the precursors erythrose-4-phosphate (E4P) and phosphoenolpyruvate (PEP). Additionally, CRISPR interference (CRISPRi) was employed to repress competing pathways and redirect flux toward MA production. High-cell-density cultivation (HCDC) in a 5 L bioreactor demonstrated the strain’s industrial potential, achieving an MA titer of 9.58 g/L, the highest reported for microbial production. This study provides a systematic metabolic engineering approach for efficient MA biosynthesis from glucose, offering a foundation for sustainable large-scale production, demonstrating not only genetic-level optimizations, but also effective process scaling through high-cell-density cultivation, highlighting the power of pathway engineering in microbial cell factories.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 6","pages":"331-331"},"PeriodicalIF":0.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Application of Transcriptome Analysis for the Exploration of the Mechanism of Methionine Promoting the Synthesis of Cephalosporin C in Acremonium chrysogenum by Employing a Chemically Defined Medium 应用转录组分析探讨蛋氨酸在化学定义培养基中促进黄顶孢菌合成头孢菌素C的机制

Fermentation Pub Date : 2025-06-06 DOI: 10.3390/fermentation11060325

Yifan Li, Zhen Chen, Wei Hong, Tao Feng, Xiwei Tian, Ju Chu

{"title":"Application of Transcriptome Analysis for the Exploration of the Mechanism of Methionine Promoting the Synthesis of Cephalosporin C in Acremonium chrysogenum by Employing a Chemically Defined Medium","authors":"Yifan Li, Zhen Chen, Wei Hong, Tao Feng, Xiwei Tian, Ju Chu","doi":"10.3390/fermentation11060325","DOIUrl":"https://doi.org/10.3390/fermentation11060325","url":null,"abstract":"To better analyze the biosynthesis mechanism of cephalosporin C (CPC) in Acremonium chrysogenum, single-factor omission experiments and Plackett–Burman (PB) experimental design were employed to identify key components in the chemically defined medium. Response surface methodology (RSM) was then applied to optimize the concentrations of critical factors, achieving a final CPC titer of 4.70 g/L, which reached 59.54% of the titer obtained with complex medium. Methionine was identified as the most significant amino acid influencing CPC production during medium optimization. On the basis of these findings, transcriptomic analysis was conducted to elucidate the regulatory role of methionine. The results revealed that methionine enhances CPC biosynthesis by upregulating cysteine metabolism-related genes and activating primary metabolic pathways to supply precursors and energy for secondary metabolism. Additionally, methionine promoted hyphal swelling and arthrospore formation, leading to the upregulated expression of genes in CPC biosynthetic gene clusters. By integrating medium optimization with transcriptomic analysis, we provided more reliable insights into the regulatory role of methionine in A. chrysogenum growth and CPC biosynthesis using a chemically defined medium, offering valuable guidance for fermentation process optimization and subsequent metabolic engineering strategies.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 6","pages":"325-325"},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/6/325/pdf?version=1749203320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Probiotic Potential, Safety, and Immunomodulatory Properties of Levilactobacillus brevis ZG2488: A Novel Strain Isolated from Healthy Human Feces 从健康人类粪便中分离的短乳酸杆菌ZG2488的益生菌潜力、安全性和免疫调节特性

Fermentation Pub Date : 2025-05-15 DOI: 10.3390/fermentation11050287

Zhijie Cao, Mengshan Chen, Yulu Chen, Hui Sun

{"title":"The Probiotic Potential, Safety, and Immunomodulatory Properties of Levilactobacillus brevis ZG2488: A Novel Strain Isolated from Healthy Human Feces","authors":"Zhijie Cao, Mengshan Chen, Yulu Chen, Hui Sun","doi":"10.3390/fermentation11050287","DOIUrl":"https://doi.org/10.3390/fermentation11050287","url":null,"abstract":"Probiotics exert beneficial effects on health improvement, infection prevention, and disease management. This study investigated the probiotic characteristics and safety parameters of Levilactobacillus brevis ZG2488, a novel strain isolated from healthy human feces. The strain exhibited robust tolerance to simulated gastrointestinal conditions, maintaining survival rates of 87.20% in artificial gastric juice (pH 3.0; 3 h) and 95.32% in 0.3% bile salt (24 h). Notably, L. brevis ZG2488 displayed superior microbial adhesion properties with high cell surface hydrophobicity (87.32%), auto-aggregation (81.15% at 24 h), and co-aggregation capacities with Escherichia coli ATCC 43895 (63.90%) and Salmonella typhimurium SL1344 (59.28%). Its adhesion to HT-29 cells (7.15%) surpassed that of the reference strain Lactobacillus rhamnosus GG (1.26%). Antimicrobial testing revealed broad-spectrum inhibitory effects against multidrug-resistant Klebsiella pneumoniae NK04152 and other pathogens. Comprehensive safety assessments confirmed the absence of hemolytic or DNase activity, along with appropriate antibiotic susceptibility to most antibiotics, except kanamycin, streptomycin, vancomycin, and penicillin G. Furthermore, L. brevis ZG2488 significantly enhanced nitric oxide production and upregulated the gene expression of nitric oxide synthase (iNOS) and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) in RAW264.7 macrophages. These findings underscore L. brevis ZG2488 as a promising probiotic candidate with functionality in pathogen inhibition and immune modulation.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 5","pages":"287-287"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-5637/11/5/287/pdf?version=1747313683","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Catabolism Mechanism and Growth-Promoting Effect of Xylooligosaccharides in Lactiplantibacillus plantarum Strain B20 植物乳杆菌B20菌株低聚木糖的分解代谢机制及促生长作用

Fermentation Pub Date : 2025-05-13 DOI: 10.3390/fermentation11050280

Yini Shi, Huan Wang, Zhongke Sun, Zifu Ni, Chengwei Li

{"title":"Catabolism Mechanism and Growth-Promoting Effect of Xylooligosaccharides in Lactiplantibacillus plantarum Strain B20","authors":"Yini Shi, Huan Wang, Zhongke Sun, Zifu Ni, Chengwei Li","doi":"10.3390/fermentation11050280","DOIUrl":"https://doi.org/10.3390/fermentation11050280","url":null,"abstract":"Prebiotics are food ingredients that result in specific changes in the composition and/or activity of the gastrointestinal microbiota, thus conferring benefits upon host health. Xylooligosaccharides (XOS) are prebiotic fibers made from xylan. Commercial XOS are mixtures of oligosaccharides containing β-1,4–linked xylose residues. Though they are widely added to foods at different doses, the molecular mechanisms of the catabolism and growth promotion of XOS in the innate gut microbes Lactobacillus spp. remain unknown. In this study, we evaluated the growth-promoting effect using a human fecal isolate, Lactiplantibacillus plantarum strain B20 (Lb. plantarum B20). Assays of bacterial growth and lactic acid production showed stronger growth promotion of XOS than other oligosaccharides did, in a dose- and fraction-dependent pattern. Using the Lb. plantarum strain SK151 genome as a reference, bioinformatic analysis failed to identify any previously characterized genes responsible for the uptake and catabolism of XOS. However, transcriptomic analysis of Lb. plantarum B20 yielded numerous differentially expressed genes (DEGs) during fermentation of XOS. Among these, an oligopeptide ABC transporter (RS03575-03595, composed of five proteins) and a hydrolase (RS06170) were significantly upregulated. Molecular docking analysis indicated that the substrate-binding protein RS03575 may mediate the import of XOS into the cell. Enzymatic assays further demonstrated that RS06170 possesses β-xylosidase activity and can effectively degrade XOS. In addition, functional enrichment analysis suggested that the growth-promoting effect of XOS may be attributed to the upregulation of genes involved in cellular component biogenesis and cell division, potentially through modulation of ribosome function and carbohydrate metabolism in Lb. plantarum B20. These results provide valuable insights into the mechanisms by which XOS promote growth and highlight potential targets for enhancing prebiotic–probiotic interactions.","PeriodicalId":507249,"journal":{"name":"Fermentation","volume":"11 5","pages":"280-280"},"PeriodicalIF":0.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1