International Journal of Applied Pharmaceutics最新文献

RESPONSE SURFACE METHODOLOGY-AIDED DEVELOPMENT OF PIRFENIDONE-LOADED SOLID LIPID NANOPARTICLES FOR INTRAPULMONARY DRUG DELIVERY SYSTEM 响应面方法辅助开发用于肺内给药系统的吡非尼酮负载固体脂质纳米颗粒

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.50231

Kevin Kwok, G. Suhariyono, Silvia Surini

{"title":"RESPONSE SURFACE METHODOLOGY-AIDED DEVELOPMENT OF PIRFENIDONE-LOADED SOLID LIPID NANOPARTICLES FOR INTRAPULMONARY DRUG DELIVERY SYSTEM","authors":"Kevin Kwok, G. Suhariyono, Silvia Surini","doi":"10.22159/ijap.2024v16i4.50231","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.50231","url":null,"abstract":"Objective: This study aims to determine the optimized Pirfenidone-loaded Solid Lipid Nanoparticles (P-SLN) formula for Intrapulmonary Drug Delivery System (IPDDS) using Response Surface Methodology (RSM).\u0000Methods: Box-Behnken design was applied to create fifteen P-SLN formulas comprising three independent variables, namely lipid-to-drug ratio, polymer type, and polymer concentration, and three dependent variables, including particle size, Polydispersity Index (PDI), and entrapment efficiency. The P-SLNs were prepared by solvent injection followed by the ultrasonication method. Those formulas were optimized with the RSM approach using the Design Expert®. Then, the optimized P-SLN was further characterized for morphology, moisture content, aerodynamic performance, and dissolution profile.\u0000Results: The optimization process, assisted by RSM, determined that the optimized P-SLN had a lipid-to-drug ratio of 6:1 and contained 0.5% Plasdone K-29/32. The resulting P-SLN had a spherical shape with a particle size of 212.7 nm, a PDI of 0.39, an entrapment efficiency of 95.02%, and a low moisture content of 1.59%. The optimized P-SLN also exhibited appropriate IPDDS required characteristics, including a Mass Median Aerodynamic Diameter (MMAD) ranging from 0.540–12.122 μm and a Respirable Fraction (RF) of 12.4%. Moreover, the release of pirfenidone from this optimized formula was 89.61% and 69.28% in pH 4.5 and 7.4 buffer media, respectively, in 45 min through a combination of diffusion and polymer swelling mechanisms.\u0000Conclusion: The optimized P-SLN showed promising potential as an IPDDS for pirfenidone.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PEPTIDE DELIVERY VIA NASAL ROUTE: EXPLORING RECENT DEVELOPMENTS AND APPROACHES 通过鼻腔途径输送多肽：探索最新进展和方法

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.50626

Chaitali Palde, Tularam Barot, G. Chakraborthy, L. D. Patel

{"title":"PEPTIDE DELIVERY VIA NASAL ROUTE: EXPLORING RECENT DEVELOPMENTS AND APPROACHES","authors":"Chaitali Palde, Tularam Barot, G. Chakraborthy, L. D. Patel","doi":"10.22159/ijap.2024v16i4.50626","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.50626","url":null,"abstract":"There has been a significant increase in interest in using the nasal route to administer peptides. This is mainly due to its advantages, including less invasiveness, rapid absorption, and the ability to bypass initial metabolism in the liver. The incorporation of nanotechnology has emerged as a prominent strategy, with nanocarriers such as nanoparticles and liposomes being employed to augment stability and bioavailability of peptides, as extensively discussed in this review. These carriers serve the crucial function of safeguarding peptides against enzymatic degradation while also enabling a sustained release, thus extending the therapeutic impact. Additionally, this review delves into mucoadhesive polymers and permeation enhancers, which have undergone extensive exploration to enhance nasal retention and augment the transportation of peptides across the nasal mucosa. Recent breakthroughs in nasal peptide delivery have heralded a new era in peptide-based therapies. These advancements encompass innovative formulation technologies, the utilization of nanocarriers, permeation enhancers, and the integration of intelligent materials and nasal drug delivery devices, all of which are geared towards enhancing the efficiency and efficacy of nasal peptide delivery.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETERMINATION OF PHARMACOKINETIC PARAMETERS FROM DISTRIBUTION STUDY FOLLOWING DEVELOPMENT AND VALIDATION OF A SENSITIVE LC-MS METHOD IN TISSUE MATRICES FOR 2-(4-ETHOXYPHENYLSULPHONAMIDO) PENTANEDIAMIDE, AN INVESTIGATIONAL ANTICANCER AGENT 在组织基质中开发和验证灵敏的 2-(4-乙氧基苯磺酰胺基)戊二酰胺（一种在研抗癌剂）lc-ms 方法后，通过分布研究确定药代动力学参数

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.51246

N. Yeasmin, Suvasish Mishra, Subrata Sen

{"title":"DETERMINATION OF PHARMACOKINETIC PARAMETERS FROM DISTRIBUTION STUDY FOLLOWING DEVELOPMENT AND VALIDATION OF A SENSITIVE LC-MS METHOD IN TISSUE MATRICES FOR 2-(4-ETHOXYPHENYLSULPHONAMIDO) PENTANEDIAMIDE, AN INVESTIGATIONAL ANTICANCER AGENT","authors":"N. Yeasmin, Suvasish Mishra, Subrata Sen","doi":"10.22159/ijap.2024v16i4.51246","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.51246","url":null,"abstract":"Objective: The aim of this study was to develop and validate bioanalytical methods for estimation of 2-(4-ethoxyphenyl sulphamido) pentanediamide (PC), an investigational anticancer agent, in various organ/tissue matrices to study various Pharmacokinetic parameters using lC-MS.\u0000Methods: Freshly prepared tissue homogenates from Sprague-Dawley rats were used as matrices to develop the bioanalytical method in lC-MS to determine Cmax, Tmax, AUC0-t, AUC0-inf, T1/2, and mean Residence Time (MRT). The distribution study was conducted by administering PC orally to Sprague-Dawley rats and quantifying PC in different excised organs at different points. A non-compartmental analysis was done using ‘PK solver’ software.\u0000Results: In all the tissue matrices, the concentrations of PC were found in the linear range of 10 to 5000 ng/ml. High level of precision, accuracy, and recovery, with negligible matrix effects, were found. PC was distributed in all tissues except the brain. Pharmacokinetic parameters such as Tmax and MRT were between 1.11±0.12 to 2.33±0.11 h and 2.17±0.16 to 4.01±0.25 h respectively in the liver, lung, heart, spleen, kidney, and thymus.\u0000Conclusion: Simple and sensitive lC-MS methods for PC in different tissue matrices were developed and validated. As PC does not cross Blood Brain Barrier (BBB), it will not adversely affect Central Nervous System (CNS). PC is absorbed fast from Gastro Intestinal Tract (GIT) to blood and subsequently reaches the different tissues. Consequently, a fast onset of action will be seen. To sum up, PC is a probable potential anticancer agent with no or minimal adverse effects on CNS.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DEVELOPMENT OF LIPID-BASED VESICLES OF TERBINAFINE GEL FOR SKIN DELIVERY BY 32FULL FACTORIAL DESIGN 通过 32 全因子设计开发用于皮肤给药的特比萘芬凝胶脂基囊泡

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.50460

T. S. Saraswathi, R. Roshini, N. Damodharan, M. Mothilal, S. K. Janani

{"title":"DEVELOPMENT OF LIPID-BASED VESICLES OF TERBINAFINE GEL FOR SKIN DELIVERY BY 32FULL FACTORIAL DESIGN","authors":"T. S. Saraswathi, R. Roshini, N. Damodharan, M. Mothilal, S. K. Janani","doi":"10.22159/ijap.2024v16i4.50460","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.50460","url":null,"abstract":"Objective: Terbinafine is a poorly water-soluble and highly permeable allylamine antifungal (BCS-II) drug. In this study, we looked at the possibility of using ethosomes as vesicular lipid nanocarriers to enhance the transdermal delivery of terbinafine.\u0000Methods: Using a 32 full factorial design, the ethosomal formulation with different soya lecithin and cholesterol concentrations was improved and optimized. The influence of independent variables, namely soya lecithin and cholesterol concentration in ethosomes was determined by estimating dependent variable including the particle size, polydispersity index, zeta potential, entrapment efficiency, and in vitro drug release. To improve the residence time of ethosomes on the topical application, the ethosomes were incorporated into the carbopol gel. 1% w/v of Carbopol 934 P gel-embedded Terbinafine ethosomes were used to study medication release and skin interactions.\u0000Results: Optimized ratios of soya lecithin and cholestrol was used to prepare vesicles. Formulation had a particle size of 1207.39±2.71 nm, entrapment efficiency of 94.46±0.47%, and in vitro diffusion of 51.27±0.16%. It was found that the growth of fungus Aspergillus niger and Candida albicans were inhibited by Ethosomal Gel. However, ethosomal gel had more inhibitory activity on Apergillus niger compared to positive control.\u0000Conclusion: The current study suggests that ethosomal vesicles may improve transdermal dispersion without causing skin irritation. Terbinafine-loaded ethosomes have the potential to be one of the most important transdermal application techniques for the treatment of fungi-related disorders.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A COMPREHENSIVE REVIEW ON THE MULTIFACETED INTERACTIONS BETWEEN HOST IMMUNITY AND VIRAL PATHOGENESIS IN COVID-19 全面综述宿主免疫与 Covid-19 病毒致病机理之间的多方面相互作用

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.50576

Mairembam Stelin Singh, Sailu Yellaboina, M. A. Ansari

{"title":"A COMPREHENSIVE REVIEW ON THE MULTIFACETED INTERACTIONS BETWEEN HOST IMMUNITY AND VIRAL PATHOGENESIS IN COVID-19","authors":"Mairembam Stelin Singh, Sailu Yellaboina, M. A. Ansari","doi":"10.22159/ijap.2024v16i4.50576","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.50576","url":null,"abstract":"The Corona Virus Disease (COVID-19) pandemic has presented unparalleled challenges, marked by a wide array of clinical presentations spanning from asymptomatic carriage to severe respiratory compromise and multi-organ dysfunction. It is crucial to comprehend the intricate interplay between host immunity and viral pathogenesis to elucidate disease mechanisms and guide therapeutic strategies. This review delves into the multifaceted interactions between host immunity and viral pathogenesis in COVID-19, with a particular focus on the impact of host factors such as age, sex, comorbidities, and genetic predisposition on disease severity. Utilizing state-of-the-art methodologies, including multiomics approaches, has yielded an expansive molecular portrayal of COVID-19, furnishing innovative perspectives on host immune reactions, viral pathogenicity, and disease advancement. Establishing standardized methodologies for data analysis and interpretation while concurrently addressing ethical considerations and promoting interdisciplinary collaboration are crucial steps in advancing our comprehension of COVID-19 pathogenesis. Despite obstacles like complexities in data integration, this review highlights the imperative of persistent endeavors in deciphering the complex interactions between hosts and pathogens to alleviate the global health ramifications of COVID-19.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FORMULATION, ANTIOXIDANT, AND ANTI-AGING ACTIVITY OF RUBUS FRAXINIFOLIUS FRACTION 红叶石楠成分的配方、抗氧化和抗衰老活性

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.51013

Sulistyowati, B. Elya, Syamsu Nur, R. Iswandana

{"title":"FORMULATION, ANTIOXIDANT, AND ANTI-AGING ACTIVITY OF RUBUS FRAXINIFOLIUS FRACTION","authors":"Sulistyowati, B. Elya, Syamsu Nur, R. Iswandana","doi":"10.22159/ijap.2024v16i4.51013","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.51013","url":null,"abstract":"Objective: The aim of this research was to evaluate the antioxidant activity and elastase inhibition of the Rubus gel fraction as well as its physical stability for 12 w. Rubus fraxinifolius leaves were reported to have strong antioxidant and elastase enzyme inhibitory activity.\u0000Methods: Water fraction from ultrasonic-assisted extraction (UAE) of old Rubus leaves was used and formulated into face gel preparations. Antioxidant activity 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2'-azino-bisphenol S (2,2'-ABPS) (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), elastase inhibition, and stability test during 12 w of storage, both at room temperature (30±2 °C) or accelerated temperature (40±2 °C) were performed.\u0000Results: The anti-aging gel showed better antioxidant activity and elastase inhibition on F1 (3%) at both conditions compared to F2 (4%) and F3 (5%). The physical stability test met the requirements. However, there was a slight decrease in antioxidant activity and elastase inhibition after 12 w of storage at 30±2 °C and 40±2 °C (F1, F2, and F3).\u0000Conclusion: The F1 met the standards and was relatively stable at 30±2 °C during 12 w of storage.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 74","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A REVERSE PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-PHOTO DIODE ARRAY ESTIMATION OF CAPTOPRIL AND HYDROCHLOROTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM 一种反相超高效液相色谱-光电二极管阵列法测定散装和药物剂型中的卡托普利和氢氯噻嗪

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.49857

M. Manoranjani, S. T. N. V. S. S., M. DAVID RAJU

{"title":"A REVERSE PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-PHOTO DIODE ARRAY ESTIMATION OF CAPTOPRIL AND HYDROCHLOROTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM","authors":"M. Manoranjani, S. T. N. V. S. S., M. DAVID RAJU","doi":"10.22159/ijap.2024v16i4.49857","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.49857","url":null,"abstract":"Objective: This investigation demonstrates a stability-indicating and reliable “Reverse Phase Ultra-Performance Liquid Chromatography (RP-UPLC)” method to simultaneously quantify Hydrochlorothiazide (HCTZ) and Captopril in the pharmaceutical dosage form.\u0000Methods: Hydrochlorothiazide and Captopril were separated by using C18 column (100 mm x 2.1 mm, 1.7μm) with an isocratic type of elution using mobile phase containing Acetonitrile+0.1% formic acid buffer (60:40), respectively with 0.2 ml/min flow rate. The wavelength used to detected at 210 nm to quantify Hydrochlorothiazide and Captopril.\u0000Results: Captopril and Hydrochlorothiazide peaks were eluted with fine resolution at retention times 0.772 min and 1.679 min, respectively. In 5-30 μg/ml concentration ranges for each Captopril and Hydrochlorothiazide, the calibration graphs were linear, with regression coefficients of 0.9998 and 0.9995, respectively. The suggested Ultra-performance liquid chromatography approach has been shown as sensitive, precise, robust, accurate, specific and stability, indicating through the resolution of Captopril and Hydrochlorothiazide from its degradation-based compounds.\u0000Conclusion: The established ultra-performance liquid chromatography technique was effectively extended to the evaluation of Captopril and Hydrochlorothiazide in the pharmaceutical dosage form, and the test results appeared satisfactory.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 73","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

REDUCTION OF TIME AND COST IN PREPARATION OF NANOFIBERS FROM PVA, PEO AND HPMC USING DESIGN-EXPERT® SOFTWARE 利用 design-expert® 软件减少用 pva、peo 和 hpmc 制备纳米纤维的时间和成本

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.50022

L. J. A. SHAWKA AL-ASADI, Sarmad Al-Edresi

{"title":"REDUCTION OF TIME AND COST IN PREPARATION OF NANOFIBERS FROM PVA, PEO AND HPMC USING DESIGN-EXPERT® SOFTWARE","authors":"L. J. A. SHAWKA AL-ASADI, Sarmad Al-Edresi","doi":"10.22159/ijap.2024v16i4.50022","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.50022","url":null,"abstract":"Objective: The following research aims to formulate nanofibers using a statistical model to reduce time and cost. Nanofibers are nanomaterials composed of a blend of more than one polymer. The selection of the proper exact ratio is challenging, costly and time-consuming.\u0000Methods: Nanofibres were prepared from polyvinyl alcohol (PVA), polyethylene oxide (PEO), and hydroxyl propyl methyl cellulose (HPMC) at different concentrations. The experiment used Design-Expert® software (version 13) through full factorial design. A high electrical field was applied to convert the polymeric solution to electrospun nanofibers. Voriconazole, as a triazole drug, was used as a model drug. The entrapment efficiency (EE%) of Voriconazole, fibre diameters and the morphology of nanofibers were analysed using scanning electron microscopy (SEM). The higher desirability of nanofibers was selected.\u0000Results: The EE% ranged from 6.7 % to 97.94 %. Fibres diameter ranged from 87.18 to 2500 nm. An SEM analysis revealed long and uniform threads of nanofibers. The solution suggested by the software out of 18 runs resulted in nanofibers having an EE% of 90.3% and a diameter of 87.8 nm±22. 2 SD.\u0000Conclusion: Electrospun nanofibres were successfully prepared from 18 runs only. A high loading of model drug was achieved at relatively low numbers of experiments. Time and cost were effectively reduced while maintaining a high desirability for the results.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BIO ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF CAPECITABINE AND DOCETAXEL AND ITS APPLICATION TO PHARMACOKINETIC STUDIES USING LC-MS/MS 采用生物分析方法同时估算卡培他滨和多西他赛及其在 lc-ms/ms 药代动力学研究中的应用

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.50125

Shaik Nagul Shareef, K. V. Padmavathi, S. Aravind, M. Subbarao

{"title":"BIO ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF CAPECITABINE AND DOCETAXEL AND ITS APPLICATION TO PHARMACOKINETIC STUDIES USING LC-MS/MS","authors":"Shaik Nagul Shareef, K. V. Padmavathi, S. Aravind, M. Subbarao","doi":"10.22159/ijap.2024v16i4.50125","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.50125","url":null,"abstract":"Objective: An easy, quick, precise, active and reproducible Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) technique was developed for the bio-analytical method of Capecitabine and Docetaxel using D9-Capecitabine and D9-Docetaxel as Internal Standards (IS).\u0000Methods: This article summarizes the recent progress on bioanalytical LC-MS/MS methods using Symmetry C18 column (150x4.6 mm, 3.5µ) and an organic mobile phase of 0.1% formic acid and Acetonitrile in 80:20 v/v.\u0000Results: Analysis was carried out within 5 min over a good linear concentration range from 37.5ng/ml to 300ng/ml (r2= 0.9999±0.008) for Capecitabine and 10ng/ml to 80ng/ml (r2=0.9993±0.005) for Docetaxel. Accuracy, precision, recovery, matrix effect and stability results were found to be within suitable limits.\u0000Conclusion: The application denotes all the parameters of system suitability, specificity, linearity and accuracy are in good agreement with United States Food and Drug Administration (USFDA) guidelines and applied effectively for the investigation of pharmacokinetic studies in rats.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141670387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SYSTEMATIC DEVELOPMENT, OPTIMIZATION AND EVALUATION OF ASCORBIC ACID-COATED SUPER-PARAMAGNETIC IRON OXIDE NANOPARTICLES (SPIONS) 抗坏血酸包覆超顺磁性氧化铁纳米粒子（spions）的系统开发、优化和评估

International Journal of Applied Pharmaceutics Pub Date : 2024-07-07 DOI: 10.22159/ijap.2024v16i4.51168

Sameea Ahmed Khan, Rajesh Sharma

{"title":"SYSTEMATIC DEVELOPMENT, OPTIMIZATION AND EVALUATION OF ASCORBIC ACID-COATED SUPER-PARAMAGNETIC IRON OXIDE NANOPARTICLES (SPIONS)","authors":"Sameea Ahmed Khan, Rajesh Sharma","doi":"10.22159/ijap.2024v16i4.51168","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i4.51168","url":null,"abstract":"Objective: In this study, Ascorbic acid-coated Super-Paramagnetic Iron Oxide Nanoparticles (AA-SPIONs) were synthesized, optimized, and further evaluated.\u0000Methods: The nanoparticles were synthesized using the co-precipitation method, optimized by Box-Behnken Design (Design Expert® software). The formulation was then characterized for several in vitro attributes such as particle size distribution, zeta potential, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Differential Scanning Calorimetry (DSC), and Vibration Sample Magnetometry (VSM).\u0000Results: An optimized formulation was designed and synthesized. It showed an average size of ~260 nm with 24 mV zeta potential. The small size and electrostatic stability suggested an even distribution of particles in the bloodstream. FTIR revealed the interaction of AA with iron oxide. XRD studies and DSC thermograms ascertained the crystallinity of the iron formulation complying that the particles behaved as a single-domain magnetic crystal. The glass transition temperature of the coated nanoparticles was found to be 135.463ºC. Vibration sample magnetometry displayed the saturation magnetization value to be 2.87 emu g‾1, which indicated the retained super-paramagnetic nature of the nanoparticles.\u0000Conclusion: The results were in concordance with the aim of this research work.","PeriodicalId":507178,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":" 37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0