Clinical and Experimental Pharmacology and Physiology最新文献

{"title":"Effects of Genetic Polymorphisms of CYP450 Enzymes on Blood Hydroxychloroquine Levels and Clinical Response Among Patients With Cutaneous Lupus Erythematosus","authors":"Jatta Njundu,&nbsp;Qi E. Chia,&nbsp;Stanslas Johnson,&nbsp;Yong Audrey Chee Hui,&nbsp;Ho Wen Chung,&nbsp;Chua Eng Wee,&nbsp;Affandi Azura Mohd,&nbsp;Lee Sut Enn,&nbsp;Wong Siu Bee,&nbsp;How Kang Nien","doi":"10.1111/1440-1681.70076","DOIUrl":"10.1111/1440-1681.70076","url":null,"abstract":"<div>\u0000 \u0000 <p>Whole blood hydroxychloroquine (WBHCQ) levels were found to correlate with cutaneous lupus (CLE) disease severity. Studies have shown HCQ to be highly variable in blood concentrations and clinical response among patients; however, the exact cause of this variation is not well understood. Genetic polymorphism of CYP450 enzymes was suspected to be a possible contributing factor. This study aimed to determine the effects of genetic polymorphism of CYP450 enzymes on clinical response to HCQ among Malaysians with CLE and its association with blood [HCQ], [DHCQ] or [HCQ]: [DHCQ] ratio. This study was a multicentre cohort study targeting CLE subjects on HCQ, recruited from five main hospitals in Malaysia. A total of 33 subjects were recruited from 1 September 2021 to 30 November 2023. Blood [HCQ], [DHCQ] or its concentration ratio and clinical response to HCQ were the outcome variables determined. Statistical Analysis showed that CYP2D6*10 was significantly associated with blood [HCQ] (<i>p</i> &lt; 0.05), while CYP3A5*3 was shown to be significantly associated with [DHCQ]: [HCQ] ratio (<i>p</i> &lt; 0.05). However, a statistically significant association was not observed between identified SNPs and clinical response to HCQ (OR: 4.444, CI: 0.770, 25.654, <i>p</i> = 0.095). These findings are aligned with those reported previously on blood HCQ and CYP polymorphism; however, clinical response to HCQ and CYP polymorphism is still debatable, as we did not observe any significant association. CYP450 genetic polymorphism significantly affects levels of HCQ in patients. Thus, individual genotypes of CYP450 enzymes may be considered for the optimum use of HCQ among CLE subjects.</p>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and Stable Mouse Model of Amphotericin B-Induced Acute Kidney Injury: Application of Continuous Non-Invasive GFR Monitoring","authors":"Lifeng Shen,&nbsp;Yuchen Song,&nbsp;Yu Xiao,&nbsp;Yu Xin,&nbsp;Yanqi Liu,&nbsp;Yuxi Liu,&nbsp;Yinghao Luo,&nbsp;Qianqian Zhang,&nbsp;Xinran Wang,&nbsp;Kaijiang Yu,&nbsp;Changsong Wang","doi":"10.1111/1440-1681.70071","DOIUrl":"https://doi.org/10.1111/1440-1681.70071","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Amphotericin B (AmB) remains a cornerstone in antifungal therapy, but its clinical use is limited by dose-dependent nephrotoxicity. Lipid-based formulations such as amphotericin B cholesterol sulfate complex dispersion (ABCD) were developed to mitigate renal injury, though their comparative renal safety profiles remain incompletely defined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to establish a pharmacologically relevant mouse model to characterise the nephrotoxicity of conventional AmB (AmB-D) and ABCD formulations, using continuous non-invasive glomerular filtration rate (GFR) monitoring and renal injury biomarkers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Male C57BL/6 mice received a single intravenous dose of AmB-D or ABCD at low or high doses. Renal function was assessed via real-time GFR monitoring, serum creatinine (SCr), blood urea nitrogen (BUN) and mRNA expression of kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL). Histopathological injury was evaluated at defined time points.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>High-dose AmB-D (2 mg/kg) and ABCD (20 mg/kg) induced rapid GFR decline within 2 h, preceding significant increases in SCr, BUN, and injury biomarkers. ABCD demonstrated 6–10-fold lower nephrotoxic potency compared to AmB-D. Low-dose groups exhibited mild, reversible changes in GFR and minimal tubular injury.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Continuous GFR monitoring enables sensitive detection of early renal dysfunction and reveals distinct nephrotoxic profiles between AmB-D and ABCD. This pharmacologically relevant model provides a powerful tool for preclinical nephrotoxicity assessment and for quantitatively comparing the renal safety of different drug formulations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycyrrhizic Acid Ameliorates LPS-Induced WI-38 Cell Inflammation, Oxidative Stress, and Ferroptosis via Targeting METTL14 in Infantile Pneumonia","authors":"Xiaofei Xie,&nbsp;Caiwen Wang,&nbsp;Yingying Sun,&nbsp;Ting Sun,&nbsp;Yongfu Song,&nbsp;Na Wang,&nbsp;Zhuang Wang,&nbsp;Yongji Wang","doi":"10.1111/1440-1681.70068","DOIUrl":"https://doi.org/10.1111/1440-1681.70068","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Infantile pneumonia is a common and significant health concern in the world, with elevated morbidity and mortality rates among affected children. This research is designed to demonstrate the therapeutic action of glycyrrhizic acid (GA) on infantile pneumonia and unravel the underlying mechanisms involved.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The models in vitro and in vivo were created to analyse the action of GA in infantile pneumonia. Human embryonic lung WI-38 cells were treated with lipopolysaccharide (LPS), and mice were administered LPS to mimic infantile pneumonia. Cell viability was tested via cell counting kit-8 (CCK8). The lactate dehydrogenase (LDH) content was examined using the LDH Cytotoxicity Assay Kit. Flow cytometry was performed to analyse cell apoptosis. The pro-inflammatory cytokine (tumour necrosis factor-alpha [TNF-a], interleukin [IL]-6 and IL-1β) levels were detected using TNF-a, IL-6 and IL-1β ELISA assay kits. The levels of ferrous ion (Fe²⁺), antioxidant glutathione (GSH), malondialdehyde (MDA) and reactive oxygen species (ROS) were analysed using corresponding assay kits. The potential target genes of GA in infantile pneumonia were predicted using molecular docking. The m6A level of mRNA was tested using the m6A RNA Methylation Assay Kit. Lung tissue pathology was analysed using haematoxylin and eosin staining.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>GA abolished LPS-induced inhibition of WI-38 cell viability and promotion of cell apoptosis, while reducing production of LDH, TNF-a, IL-6 and IL-1β. Besides, GA suppressed the levels of Fe²⁺, MDA and ROS and facilitated the GSH, solute carrier family 7 member 11 (SLC7A11), and glutathione peroxidase 4 (GPX4) levels. The molecular docking predicted that the methyltransferase-like 14 (METTL14) was a potential target of GA and had good bonding ability. Interestingly, METTL14 expression was promoted in LPS-stimulated WI-38 cells and the serum of patients with infantile pneumonia. GA repressed cell apoptosis, levels of LDH, TNF-a, IL-6, IL-1β, Fe²⁺, MDA and ROS, and facilitated the GSH, SLC7A11 and GPX4 levels of LPS-induced WI-38 cells by METTL14 silence. GA abrogated lung injury of LPS-induced mice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>GA alleviates LPS-induced WI-38 cell cytotoxicity, inflammation, oxidative stress and ferroptosis by METTL14 knockdown. Our findings suggest that GA may pave the way for the treatment of infantile pneumonia.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Analysis of Pulmonary Nontuberculous Mycobacterial Infection in Patients With Pre-Existing Stage II–IV Lung Cancer","authors":"Juan Li,&nbsp;Junsheng Fan,&nbsp;Zhili Tan,&nbsp;Anqi Li,&nbsp;Siyuan He,&nbsp;Yaping Jia,&nbsp;Rong Li,&nbsp;Yani Lin,&nbsp;Zihao Liu,&nbsp;Haiqing Chu,&nbsp;Zhemin Zhang","doi":"10.1111/1440-1681.70060","DOIUrl":"https://doi.org/10.1111/1440-1681.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>Immune checkpoint inhibitors (ICIs) targeting programmed cell death-1 (PD-1) and programmed cell death ligand-1 (PD-L1) have improved cancer outcomes but can induce immune-related adverse events, including mycobacterial infections. This study retrospectively analysed pulmonary nontuberculous mycobacteria (NTM) infection in 91 patients with pre-existing stage II–IV lung cancer at Shanghai Pulmonary Hospital between June 2015 and June 2024. Among these patients, 78.0% [71/91] were male, and 16.5% [15/91] were receiving ICIs monotherapy at the time of initial pulmonary NTM infection diagnosis (ICIs group). Compared with the non-ICIs group, ICIs-treated patients developed pulmonary NTM infection earlier (median time: 12 months [IQR, 6–18] vs. 21.5 months [IQR, 13–30]; <i>p</i> = 0.004), and had a higher proportion of rapid-growing NTM isolates (73.3% [11/15] vs. 30.3% [23/76]; <i>p</i> = 0.003), predominantly <i>Mycobacterium abscessus</i> complex (40.0% [6/15]). In contrast, <i>Mycobacterium avium</i> complex was the predominant NTM species in the non-ICIs group (71.1% [54/76]). During 12 months of anti-NTM therapy, the ICIs group showed a lower cumulative sputum culture conversion rate (36.4% [4/11] vs. 59.6% [31/52]; <i>p</i> = 0.19) and a longer median time to initial sputum culture conversion (12 months vs. 6 months; <i>p</i> = 0.13), though these differences were not statistically significant. The most commonly administered ICIs drugs were tislelizumab (40.0% [6/15]) and sintilimab (26.7% [4/15]). These findings suggest that ICIs may be associated with earlier development of pulmonary NTM infection in lung cancer patients. Future prospective studies with larger sample sizes are needed to validate this conclusion.</p>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic Acid Improves Salicylic Acid-Induced Cochlear Injury by Reducing Oxidative Stress and Inflammatory Response Through TLR4/NF-κB Signalling Pathway","authors":"Yi Lin,&nbsp;Lili Li,&nbsp;Jianbin Cheng","doi":"10.1111/1440-1681.70067","DOIUrl":"https://doi.org/10.1111/1440-1681.70067","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sodium salicylate (SS) causes hearing damage and tinnitus in humans and animals. Chlorogenic acid (CGA) has strong antioxidant and anti-apoptotic effects, but whether it can protect the cochlea is unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>SS was used to induce cochlear injury in rats, followed by 150, 300 and 600 mg/kg CGA treatment. The changes of tinnitus behaviour in each group were detected by auditory brainstem response (ABR), behavioural test, beam balance, Preyer reflex and auditory startle test. The levels of oxidative stress and inflammation were detected by kit. The pathological damage of cochlea was detected by HE staining, immunofluorescence staining, succinate dehydrogenase staining, and toluidine blue staining. The apoptosis of outer hair cell and spiral ganglion cell and TLR4/NF-κB pathway were detected by TUNEL staining, immunohistochemistry, immunofluorescence and Western blot.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After SS induction, the ABR threshold, EP score and beam balance score of the rats increased significantly, and the AA score, Preyer reflex and auditory startle response decreased significantly; that is, the cochlear injury model was successfully constructed. Together, the levels of oxidative indexes (ROS, MDA) and inflammatory indexes (TNF-α, IL-1β) in rats after cochlear injury were significantly increased, and the spiral ganglion, organ of Corti, outer hair cells and nerve fibres of cochlear tissue were severely damaged. The outer hair cell and spiral ganglion cell were significantly apoptotic, and the number of TLR4 positive cells, NF-κB p65 fluorescence intensity and protein level in the cochlea of rats were significantly increased. However, after CGA treatment, the above indicators were significantly improved.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CGA can reduce SS-induced oxidative stress and inflammatory response in the cochlea through restraining the TLR4/NF-κB pathway, improve the pathological damage of the cochlea and reduce ototoxicity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping Nutrition and Tuberculosis Research: Insights From Bibliometric Perspective","authors":"Chenqi Li,&nbsp;Biao Gao,&nbsp;Hongmei Xiao,&nbsp;Wenjing Shi,&nbsp;Hongtao Lu,&nbsp;Gen Miao,&nbsp;Xiaohua Tu,&nbsp;Yuxiao Tang,&nbsp;Hui Shen","doi":"10.1111/1440-1681.70069","DOIUrl":"https://doi.org/10.1111/1440-1681.70069","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Malnutrition and tuberculosis form a mutually reinforcing vicious cycle. While nutritional interventions are crucial for TB management, the knowledge structure and research frontiers remain insufficiently characterised.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To systematically analyse the structure, trajectory and frontiers of research in the nutrition-tuberculosis field using bibliometric methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Relevant literature published since 2007 was retrieved from the Web of Science Core Collection database. CiteSpace was employed to perform multidimensional analyses, including co-occurrence, cluster timeline visualisation and burst detection for keywords, citations and authors, thereby constructing knowledge maps and identifying key nodes through network centrality metrics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 4502 bibliographic records were analysed. Key findings include: (1) Vitamin D occupies a central position (frequency 326, centrality 70), bridging basic immune mechanisms and clinical applications; (2) research paradigms evolved from molecular mechanism exploration (2007–2012), through clinical translation validation (2011–2019), to systems biology integration (2019–2025); (3) gut microbiota (burst strength 11.73) and (fatty) acids emerged as frontiers; (4) diabetes–tuberculosis comorbidity revealed the complexity of metabolic-immune interaction networks and (5) high citation frequency of WHO reports indicates a pressing need for translating research into policy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Nutrition–tuberculosis research is shifting from single-nutrient studies towards integrated ‘nutrition–microbiome–metabolism–immunity’ networks. Vitamin D remains central, but future priorities should focus on precision interventions, multi-omics integration and translation from mechanism to practice, especially for high-risk groups.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold-Containing Compound Auranofin Ameliorates 6-OHDA Induced Neurotoxicity in Rats: Via Targeting PI3K/Akt/GSK-3β Signalling","authors":"Divya Soni,&nbsp;Manjinder Singh,&nbsp;Yogesh Garg,&nbsp;Amit Bhatia,&nbsp;Puneet kumar","doi":"10.1111/1440-1681.70070","DOIUrl":"https://doi.org/10.1111/1440-1681.70070","url":null,"abstract":"<div>\u0000 \u0000 <p>The prevalence of parkinsonism is increasing worldwide, not only in age groups but now becoming a favourite of adults. As the onset is increasing in developed and underdeveloped countries, the current therapy lacks a permanent cure. Therefore, the research has used the gold-containing compound auranofin (AUF) in its neuroprotective form. The research was conducted to decipher the neuroprotective potential of AUF hybrid nanoparticles (AUFHNPs) using the in silico and in vivo 6-OHDA models. The in silico study was performed using CDOCKER software and the binding affinity of AUF with PI3K (5DXT), AKT (1UNQ), GSK-3β (1Q41), Nrf2 (2DYH), and HO-1 (1N45) were assessed. Then, intra-striatal unilateral injection of 6-OHDA (10 μg/2 μL) was given to rats with the help of stereotaxic surgery. After performing the amphetamine challenge test, rodents were treated with AUF (10 mg/kg) and AUFHNPs (5 and 10 mg/kg) for 21 days. All the behaviour parameters were performed on the last 2 days, and animals were sacrificed; brains were isolated for oxidative stress, neuroinflammatory, apoptotic, histological, and molecular marker analysis. In the docking study, AUF offered the highest binding score of −61.1231 with GSK-3β. In vivo administration of AUF and AUFHNPs significantly restored motor and behaviour alterations observed in open field tests, narrow beam walks, and grip strength meter. It also restored morphological changes and increased expression of pPI3K/pAKT, followed by inhibition of GSK-3β. Thus, the AUFHNPs were more significant than AUF alone and exerted neuroprotection via modulation of PI3K/AKT/GSK-3β signalling pathways.</p>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: An MSC Bone-Homing Compound, Rab001, Increases Bone Mass and Reduces the Incidence of Osteonecrosis in a Glucocorticoid-Induced Osteonecrosis Mouse Model","authors":"","doi":"10.1111/1440-1681.70066","DOIUrl":"https://doi.org/10.1111/1440-1681.70066","url":null,"abstract":"<p>\u0000 <b>RETRACTION</b>: <span>M. Jiang</span>, <span>L. Liu</span>, <span>X. Xiang</span>, <span>R. Liang</span>, <span>X. Qin</span>, <span>J. Zhao</span>, and <span>Q. Wei</span>, “ <span>An MSC Bone-Homing Compound, Rab001, Increases Bone Mass and Reduces the Incidence of Osteonecrosis in a Glucocorticoid-Induced Osteonecrosis Mouse Model</span>,” <i>Clinical and Experimental Pharmacology and Physiology</i> <span>48</span>, no. <span>5</span> (<span>2020</span>): <span>770</span>–<span>781</span>, https://doi.org/10.1111/1440-1681.13441.\u0000 </p><p>The above article, published online on 15 December 2020 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Yang Yang; and John Wiley &amp; Sons Australia Ltd. A third party reported concerns regarding the misreporting of Chi-square test results in this article, which originally lead to the conclusion that Rab001 significantly reduces ON incidence in GC-treated rats. The third party also indicated that the article includes several methodological issues that put into question the validity of the reported results. The authors responded to an inquiry by the publisher and provided further statistical analysis and explanations for the potential methodological shortcomings. The authors acknowledged that the statistical significance in the title, abstract, and results was overstated and proposed an erratum to update all statements which reference the significance of Rab001 reducing the incidence of ON. Upon review of the authors' proposed erratum, the journal found that the proposed modifications were extensive and would significantly impact the core findings of the article. The retraction has been agreed to because the misreported statistical significance of the findings constitutes a major error, which compromises the conclusions of the article. The authors agree with the retraction.</p>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1440-1681.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Associated Factors of Anxiety and Depression in Lung Cancer Patients","authors":"Xinran Gao,&nbsp;Maoying Guan,&nbsp;Bing Bo,&nbsp;Wencheng Zhao,&nbsp;Lihua Huang,&nbsp;Yayi He","doi":"10.1111/1440-1681.70065","DOIUrl":"https://doi.org/10.1111/1440-1681.70065","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>Given the prevalence of anxiety and depression amongst lung cancer patients in China, it is critical to identify potential factors contributing to these symptoms to improve future treatment strategies. However, current research has primarily focused on clinical variables, leaving various sociodemographic factors largely underexplored. Examining these aspects is essential for enhancing clinical interventions and patient care, as sociodemographic factors can significantly influence psychological outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 486 lung cancer patients were included in the study. Data on anxiety and depression were collected using the Hospital Anxiety and Depression Scale (HADS), and sociodemographic information was gathered via a structured questionnaire. Clinical data was retrieved from the hospital's database. Univariate analysis and multivariate logistic regression were applied to identify sociodemographic and clinical factors that were significantly associated with anxiety and depression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The findings revealed prevalence rates of 24.07% for anxiety and 25.72% for depression. Local residency in Shanghai, internet use, financial strain, and advanced cancer stages (III or IV) were associated with a higher level of both anxiety and depression. Having a university/college education or higher was solely linked with increased anxiety levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Local residency, internet use, financial strain, cancer stage, and educational background are key predictors of anxiety and depression amongst lung cancer patients. It is crucial for healthcare professionals to monitor and support the mental well-being of lung cancer patients, especially those affected by these identified factors.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 10","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144843278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Semisynthetic Derivative of Dehydrozingerone (DHZ-15) Modulates Lipopolysaccharide-Stimulated Macrophages by Targeting the NF-κB/p65 Pathway and In Vivo Evaluation in a Sepsis BALB/c Model","authors":"Irfan Qasam,&nbsp;Shah Nawaz,&nbsp;Chetan Kumar,&nbsp;Hema Kumari,&nbsp;Sumit Dhiman,&nbsp;Priya Wazir,&nbsp;Govind Yadav","doi":"10.1111/1440-1681.70063","DOIUrl":"https://doi.org/10.1111/1440-1681.70063","url":null,"abstract":"<div>\u0000 \u0000 <p>Natural products and their semisynthetic derivatives possess tremendous medicinal properties and have the potential to modulate the immune system, providing new therapeutic options for drug development. In this study, we evaluated Dehydrozingerone-15, a novel dehydrozingerone derivative, for its anti-inflammatory and antioxidant properties through standardised in vitro and in vivo approaches. Dehydrozingerone-15 suppressed the stimulatory effect of LPS in RAW 264.7 cells by reducing the secretion of interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2) and nitric oxide. Western blot analysis at the mechanistic level showed a reduced expression level of nitric oxide synthase (iNOS), IκB kinase beta (Ikk-β) and nuclear factor kappa-B (NF-κB/p65). Confocal microscopy studies further demonstrated that Dehydrozingerone-15 reduced the expression of NF-κB/p65 markedly. In the in vivo LPS-induced sepsis model, Dehydrozingerone-15 administration reduced TNF-α and IL-6 expression and protected vital organs (lungs, kidneys, and liver) from acute inflammation. The anti-inflammatory potential of Dehydrozingerone-15 was further validated in leukocyte migration induced by carrageenan and vascular permeability triggered by acetic acid assays, both of which showed significant inhibition. Pharmacokinetic analysis revealed that Dehydrozingerone-15 was rapidly absorbed in BALB/c mice, reaching a <i>C</i>_max of 10 349 ng/mL at 0.25 h. The total drug exposure (AUC_0–α) was 13 862 ng.h/mL, indicating sustained exposure, with high tissue distribution (20 L/kg) and moderate clearance. Additionally, toxicological evaluation at doses up to 2000 mg/kg body weight showed no significant alterations in haematological parameters compared with the vehicle control. Furthermore, based on a comparative evaluation of in vitro and in vivo results, Dehydrozingerone-15, relative to its parent molecule, demonstrates significant therapeutic potential with high efficacy against inflammation and oxidative stress.</p>\u0000 </div>","PeriodicalId":50684,"journal":{"name":"Clinical and Experimental Pharmacology and Physiology","volume":"52 9","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}