Clinical and Investigative Medicine最新文献

The Impact of miR-34a on Endothelial Cell Viability and Apoptosis in Ischemic Stroke: Unraveling the MTHFR-Homocysteine Pathway. 缺血性中风中 miR-34a 对内皮细胞活力和凋亡的影响：揭秘 MTHFR-同型半胱氨酸通路

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-09-01 Epub Date: 2024-08-27 DOI: 10.3138/cim-2024-2711

Lina Liang, Xueli Yi, Chunfang Wang, Li Su, Guijiang Wei

{"title":"The Impact of miR-34a on Endothelial Cell Viability and Apoptosis in Ischemic Stroke: Unraveling the MTHFR-Homocysteine Pathway.","authors":"Lina Liang, Xueli Yi, Chunfang Wang, Li Su, Guijiang Wei","doi":"10.3138/cim-2024-2711","DOIUrl":"10.3138/cim-2024-2711","url":null,"abstract":"Introduction: Ischemic stroke (IS) is a global health concern, often tied to dyslipidemia and vascular endothelial dysfunction. MicroRNA-34a (miR-34a) was reported to be up-regulated in the blood samples of patients with IS, but the specific role of miR-34a and methylenetetrahydrofolate reductase (MTHFR) in IS remains to be elucidated.Methods: We studied 143 subjects: 71 IS patients, and 72 healthy controls. Human umbilical vein endothelial cells (HUVECs) were cultured and transfected with a miR-34a mimic, inhibitor, or negative control. The miR-34a expression in serum and HUVECs was quantified via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Viability and apoptosis of HUVECs were assessed using CCK-8 assay and flow cytometry. The expression levels of bcl-2, bax, cyt-c, cleaved caspase 3, MTHFR, and homocysteine were measured by Western blot or enzyme-linked immunosorbent assay (ELISA). The relationship between miR-34a and MTHFR was verified by luciferase reporter assay. The levels of MTHFR and homocysteine in serum were examined by ELISA.Results: MiR-34a expression was increased in IS patients and inhibited viability of HUVECs while promoting their apoptosis. Overexpression of miR-34a up-regulated pro-apoptotic proteins (bax, cyt-c and cleaved caspase 3) and down-regulated anti-apoptotic protein bcl-2 in HUVECs. MTHFR was identified as the downstream target of miR-34a and its expression was reduced by miR-34a overexpression, while homocysteine levels increased. Consistently, MTHFR levels were lower and homocysteine levels were higher in IS patients compared with controls.Discussion: Our results suggest that up-regulated miR-34a plays a role in the pathogenesis of IS, potentially through inhibiting MTHFR expression and increasing homocysteine in endothelial cells. Therefore, miR-34a might be a therapeutic target for IS.","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newsletter Fall 2024: Clinician Investigator Trainee Association of Canada (CITAC). 2024 年秋季通讯：加拿大临床研究员受训者协会（CITAC）。

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-09-01 DOI: 10.3138/cim-47-3-news

MohdWessam Al Jawhri, Salonee V Patel, Teija Bily, Yujin Suk, Robert X Lao

引用次数: 0

Young Investigator Interview with CSCI Awardee, Dr. Kenneth Rockwood. 与 CSCI 获奖者 Kenneth Rockwood 博士的青年研究员访谈。

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-09-01 DOI: 10.3138/cim-47-3-award

Salonee V Patel, Kenneth Rockwood

引用次数: 0

Clinical Application of CT Imaging Grading System in Upper Urinary Tract Calculi with Kidney Infection. CT 成像分级系统在上尿路结石合并肾脏感染中的临床应用

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-09-01 DOI: 10.3138/cim-2024-0107

Jianping Zhang, Lingfeng Zhu, Xiaoxia Wu, Haiying Chen, Runyang Pan, Zihuang Hong, Rongkai Lin

{"title":"Clinical Application of CT Imaging Grading System in Upper Urinary Tract Calculi with Kidney Infection.","authors":"Jianping Zhang, Lingfeng Zhu, Xiaoxia Wu, Haiying Chen, Runyang Pan, Zihuang Hong, Rongkai Lin","doi":"10.3138/cim-2024-0107","DOIUrl":"https://doi.org/10.3138/cim-2024-0107","url":null,"abstract":"Purpose: This study aimed to establish a CT imaging grading system and explore its value in evaluating upper urinary tract calculi associated with kidney infections.Methods: CT images of 126 patients with kidney infections caused by upper urinary tract calculi were retrospectively analyzed. The CT grading system was developed based on CT images. CT images were classified into 4 grades. General information, symptoms, and clinical findings of patients in different CT grades were analyzed. With the occurrence of systemic inflammatory response syndrome (SIRS) as the endpoint, univariate and multivariate analysis was conducted to analyze the risk factors of SIRS.Results: Patients with fever or diabetes had higher CT grades, and the following examination data revealed significant differences across the various CT grades (P < 0.05): the white blood cell count, urine leucocytes count, CT1, CT2, maximum body temperature, duration of disease, the proportion of blood neutrophils, the size of stones, and levels of the C-reactive protein and procalcitonin. Only CT grading was statistically significant after multivariate analysis. According to the values of the partial regression coefficient (B), the higher the CT grade, the greater the risk of SIRS. The risk of SIRS was 4.472 times higher with each increment of the CT grade.Conclusions: The CT grade is directly associated with clinical symptoms and the risk of SIRS.","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Trends and Frontier in Research on Pancreatic Alpha Cells: A Bibliometric Analysis from 2013 to 2023. 胰腺α细胞研究的全球趋势和前沿：2013年至2023年文献计量分析》。

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-06-01 DOI: 10.3138/cim-2024-2744

Teng Guo, Haoling Zhang, Yunpeng Luo, Xi Yang, Lidan Wang, Guangde Zhang

{"title":"Global Trends and Frontier in Research on Pancreatic Alpha Cells: A Bibliometric Analysis from 2013 to 2023.","authors":"Teng Guo, Haoling Zhang, Yunpeng Luo, Xi Yang, Lidan Wang, Guangde Zhang","doi":"10.3138/cim-2024-2744","DOIUrl":"https://doi.org/10.3138/cim-2024-2744","url":null,"abstract":"Purpose: Over the past 20 years, much of the research on diabetes has focused on pancreatic beta cells. In the last 10 years, interest in the important role of pancreatic alpha cells in the pathogenesis of diabetes, which had previously received little attention, has grown. We aimed to summarize and visualize the hotspot and development trends of pancreatic alpha cells through bibliometric analysis and to provide research direction and future ideas for the treatment of diabetes and other islet-related diseases.Methods: We used two scientometric software packages (CiteSpace 6.1.R6 and VOSviewer1.6.18) to visualize the information and connection of countries, institutions, authors, and keywords in this field.Results: A total of 532 publications, published in 752 institutions in 46 countries and regions, were included in this analysis. The United States showed the highest output, accounting for 39.3% of the total number of published papers. The most active institution was Vanderbilt University, and the authors with highest productivity came from Ulster University. In recent years, research hotspots have concentrated on transdifferentiation, gene expression, and GLP-1 regulatory function. Visualization analysis shows that research hotspots mainly focus on clinical diseases as well as physiological and pathological mechanisms and related biochemical indicators.Conclusions: This study provides a review and summary of the literature on pancreatic alpha cells through bibliometric and visual methods and shows research hotspot and development trends, which can guide future directions for research.","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recommendations for Recovery of the COVID-19 Pandemic-related Diagnostic, Screening, and Procedure Backlog in Ontario: A Survey of Healthcare Leaders. 关于恢复安大略省 COVID-19 大流行相关诊断、筛查和手术积压的建议：医疗保健领导者调查。

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-06-01 DOI: 10.3138/cim-2024-2655

Teagan T Telesnicki, Andrea N Simpson, Charles de Mestral, Nancy N Baxter, David R Urbach, David Gomez

{"title":"Recommendations for Recovery of the COVID-19 Pandemic-related Diagnostic, Screening, and Procedure Backlog in Ontario: A Survey of Healthcare Leaders.","authors":"Teagan T Telesnicki, Andrea N Simpson, Charles de Mestral, Nancy N Baxter, David R Urbach, David Gomez","doi":"10.3138/cim-2024-2655","DOIUrl":"https://doi.org/10.3138/cim-2024-2655","url":null,"abstract":"Purpose: The COVID-19 pandemic has resulted in a significant diagnostic, screening, and procedure backlog in Ontario. Engagement of key stakeholders in healthcare leadership positions is urgently needed to inform a comprehensive provincial recovery strategy.Methods: A list of 20 policy recommendations addressing the diagnostic, screening and procedure backlog in Ontario were transformed into a national online survey. Policy recommendations were rated on a 7-point Likert scale (strongly agree to strongly disagree) and organized into those retained (≥75% strongly agree to somewhat agree), discarded (≥80% somewhat disagree to strongly disagree), and no consensus reached. Survey participants included a diverse sample of healthcare leaders with the potential to impact policy reform.Results: Of 56 healthcare leaders invited to participate, there were 34 unique responses (61% response rate). Participants were from diverse clinical backgrounds, including surgical subspecialties, medicine, nursing, and healthcare administration and held institutional or provincial leadership positions. A total of 11 of 20 policy recommendations reached the threshold for consensus agreement with the remaining 9 having no consensus reached.Conclusion: Consensus agreement was reached among Canadian healthcare leaders on 11 policy recommendations to address the diagnostic, screening, and procedure backlog in Ontario. Recommendations included strategies to address patient information needs on expected wait times, expand health and human resource capacity, and streamline efficiencies to increase operating room output. No consensus was reached on the optimal funding strategy within the public system in Ontario or the appropriateness of implementing private funding models.","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spring 2024: Clinician Investigator Trainee Association of Canada (CITAC). 2024 年春：加拿大临床研究员受训者协会（CITAC）。

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-06-01 DOI: 10.3138/cim-2024-2740

MohdWessam Al Jawhri, Salonee V Patel, Robert X Lao

引用次数: 0

Training Outcomes and Satisfaction in Canadian MD/PhD and MD/MSc Programs: Findings from a National Survey. 加拿大 MD/PhD 和 MD/MSc 项目的培训成果和满意度：全国调查的结果。

IF 1.2 4区医学

Clinical and Investigative Medicine Pub Date : 2024-06-01 DOI: 10.3138/cim-2024-2714

Mimosa Luigi, Charles Yin, Ehsan Misaghi, Robert X Lao, Bryce J M Bogie, Jillian Macklin, Andy Zeng, Nicola Jones

{"title":"Training Outcomes and Satisfaction in Canadian MD/PhD and MD/MSc Programs: Findings from a National Survey.","authors":"Mimosa Luigi, Charles Yin, Ehsan Misaghi, Robert X Lao, Bryce J M Bogie, Jillian Macklin, Andy Zeng, Nicola Jones","doi":"10.3138/cim-2024-2714","DOIUrl":"https://doi.org/10.3138/cim-2024-2714","url":null,"abstract":"Purpose: Despite the impact of physician-scientists on scientific discovery and translational medicine, several reports have signalled their declining workforce, reduced funding, and insufficient protected research time. Given the paucity of outcome data on Canadian MD/PhD programs, this study presents a national portrait of the sociodemographic characteristics, training trajectories, productivity, and satisfaction in trainees and alumni from Canadian MD/PhD and MD/MSc programs.Methods: Quantitative data were collected in a national survey launched in 2021. Respondents included 74 MD/PhD alumni and 121 trainees across 12 Canadian MD/PhD and MD/MSc programs.Results: Among MD/PhD alumni, 51% were independent practitioners/researchers while others underwent residency training. Most trainees (88%) were in MD/PhD programs. Significantly more alumni identified as men than did trainees. Significantly more alumni conducted clinical and health services research, while more trainees conducted basic science research. Average time to MD/PhD completion was 8 years, with no correlation to subsequent research outcomes. Self-reported research productivity was highest during MD/PhD training. Concerning training trajectories, most alumni completed residency, pursued additional training, and practised in Canada. Finally, regression models showed that trainees and alumni were satisfied with programs, with significant moderators in trainee models.Conclusion: Survey findings showed Canadian MD/PhD and MD/MSc programs recruit more diverse cohorts of trainees than before, provide productive research years, and graduate alumni who pursue training and academic employment in Canada. Both alumni and trainees are largely satisfied with these training programs. The need to collect in-depth longitudinal data on Canadian MD/PhD graduates to monitor diversity and success metrics is discussed.","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Value of serum iron and urine neutrophil gelatinase-associated lipocalin in predicting the mortality of critically ill patients with sepsis. 血清铁和尿液中性粒细胞明胶酶相关脂褐质在预测脓毒症重症患者死亡率方面的价值。

IF 0.8 4区医学

Clinical and Investigative Medicine Pub Date : 2024-03-01 DOI: 10.3138/cim-2024-2672

Rui Zhao, Jiang-Hua Liu, Ze-Liang Qiu

{"title":"Value of serum iron and urine neutrophil gelatinase-associated lipocalin in predicting the mortality of critically ill patients with sepsis.","authors":"Rui Zhao, Jiang-Hua Liu, Ze-Liang Qiu","doi":"10.3138/cim-2024-2672","DOIUrl":"10.3138/cim-2024-2672","url":null,"abstract":"Introduction: We aimed to investigate the association of iron metabolism-related parameters with 60-day mortality in critically ill patients with sepsis.Methods: Serum or urine concentrations of iron metabolism-related parameters on intensive care unit admission were measured in a prospective cohort of 133 eligible patients with sepsis according to the Sepsis-3 criteria, and these values were compared between survivors and nonsurvivors, categorized according to their 60-day survival status. Cox regression analyses were performed to examine the association between iron parameters and 60-day mortality. Kaplan-Meier methods were used to illustrate the differences in survival between different iron parameters.Results: Of the 133 patients included in the study, 61 (45.8%) had died by day 60. After adjusting for confounding variables, higher concentrations of serum iron (cut-off 9.5 μmol/mL) and higher concentrations of urine neutrophil gelatinase-associated lipocalin (uNGAL; cut-off 169.3 ng/mL) were associated with a significantly greater risk of death in the Cox regression analysis. These two biomarkers combined with Sequential Organ Failure Assessment (SOFA) scores increased the area under the receiver operating characteristic (AUROC) curve to 0.85.Discussion: These findings suggest that higher concentrations of serum iron and uNGAL are each associated with higher 60-day mortality, and they add significant accuracy to this prediction in combination with SOFA. Abbreviations: uNGAL: urine neutrophil gelatinase-associated lipocalin; ICU: intensive care unit; SOFA: Sequential Organ Failure Assessment; APACHE II: the Acute Physiology and Chronic Health Evaluation II; ELISA: enzyme-linked immunosorbent assay; HR: hazard ratio; CIs: confidence intervals; WBC: white blood cell; TBIL: total bilirubin.","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140307708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Discussion with Dr. Natasha Kekre, Hematologist and Clinician Scientist. 与血液病学家兼临床科学家娜塔莎-凯克尔博士的讨论。

IF 0.8 4区医学

Clinical and Investigative Medicine Pub Date : 2024-03-01 DOI: 10.3138/cim-2024-2657

Amelia T Yuan, Natasha Kekre

引用次数: 0