Clinical and Investigative Medicine最新文献

{"title":"In Recognition of Dr. Robert Bortolussi.","authors":"Brent W Winston","doi":"10.3138/cim-48-1-editorial","DOIUrl":"https://doi.org/10.3138/cim-48-1-editorial","url":null,"abstract":"","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"48 1","pages":"1"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Ferritin Combined with Glycated Hemoglobin for Early Prediction of Gestational Diabetes Mellitus: A Retrospective Cohort Study.","authors":"Wei Wang, Yu-Ping Wei, Yu-Qi Zhang, Sheng-Hu Miao, Xiang Chang","doi":"10.3138/cim-2024-0101","DOIUrl":"https://doi.org/10.3138/cim-2024-0101","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the value of serum ferritin (SF) in conjunction with glycated hemoglobin (HbA_1c) for the early prediction of gestational diabetes mellitus (GDM) and to provide insights that could enhance health care standards for women and newborns.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted involving 650 pregnant women who received regular prenatal check-ups at our institution from January 2019 to April 2024. Participants were categorized into four groups based on their SF concentration quartiles during the 11th to 13th weeks of gestation. Logistic regression analyses were conducted to assess the predictive value of early GDM risk factors, with the lowest quartile group serving as a reference.</p><p><strong>Results: </strong>The incidence rate of GDM rose progressively with increasing SF concentrations at 11-13 weeks of gestation, with rates of 18.79%, 21.25%, 24.38%, and 25.45% respectively. Notably, the incidence rate in the highest quartile group (quartile 4) was significantly higher compared to the lowest (quartile 1), with an odds ratio of 1.48 and a 95% confidence interval of 1.12 to 1.93. Additionally, the predictive model incorporating both SF concentration and HbA_1c (Model 2) outperformed the model with SF alone (Model 1), indicating a heightened predictive accuracy for GDM when these two biomarkers are used in combination.</p><p><strong>Conclusion: </strong>The findings of this study highlight the potential utility of SF and HbA_1c as early predictors of GDM risk, especially when employed in combination.</p>","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"48 1","pages":"5-10"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crocin ameliorates hypertension-induced cardiac hypertrophy and apoptosis by activating AMPKα signalling.","authors":"Dan Luo, Jueyan Wang, Shijiao Zheng, Wei Li, Bo Yu, Huan Peng, Feng Gui, Bing Mao, Zhen Chen","doi":"10.3138/cim-2024-0118","DOIUrl":"https://doi.org/10.3138/cim-2024-0118","url":null,"abstract":"<p><strong>Purpose: </strong>Cardiac hypertrophy is a critical contributor to heart failure. Therapies that effectively manage cardiac hypertrophy are still inadequate. Crocin is a natural component of saffron, and its beneficial properties have been previously documented. This study aimed to investigate the role of crocin in cardiac hypertrophy and apoptosis and its related mechanisms.</p><p><strong>Methods: </strong>Sprague-Dawley rats were infused with angiotensin II (Ang II; 520 ng/kg/min) or normal saline and then intraperitoneally injected with crocin (40 mg/kg) or dimethyl sulfoxide for 4 weeks. Systolic and diastolic blood pressure were recorded. Cardiac hypertrophy was evaluated by echocardiography, heart weight, hematoxylin-eosin staining, TUNEL assay, and gene expression. For in vitro studies, H9C2 cells were treated with Ang II (1 μM) for 48 hours to induce cardiac hypertrophy-like conditions. An immunofluorescence assay was used for [Formula: see text]-actinin staining. reverse transcription quantitative real-time polymerase chain reaction was performed to measure the expression of hypertrophic markers, and western blotting was used to detect apoptosis and underlying mechanisms.</p><p><strong>Results: </strong>Our findings revealed that crocin attenuated diastolic dysfunction, cardiac hypertrophy, and apoptosis caused by Ang II in vivo. Additionally, crocin prevented Ang II-stimulated cardiomyocyte enlargement and apoptosis in vitro. Mechanistically, crocin induced AMP-activated protein kinase (AMPK)[Formula: see text] activation and mTOR/p70S6K inhibition in cellular and animal models of cardiac hypertrophy. Moreover, AMPK inhibition abolished the anti-hypertrophic effect of crocin in vitro, while mTOR inhibition enhanced the protective effect of crocin against Ang II-induced cardiomyocyte hypertrophy.</p><p><strong>Conclusion: </strong>This study demonstrates that crocin can ameliorate Ang II-stimulated cardiac hypertrophy in vivo and in vitro by regulating AMPK[Formula: see text]/mTOR/ p70S6K signalling.</p>","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"48 1","pages":"11-23"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"President's Letter.","authors":"Michael Bezuhly","doi":"10.3138/cim-48-1-letter","DOIUrl":"https://doi.org/10.3138/cim-48-1-letter","url":null,"abstract":"","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"48 1","pages":"2"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Interleukin Levels Predict Occurrence of Acute Radiation Pneumonitis and Overall Survival in Thoracic Tumours.","authors":"Yan Zhang, Wen-Xiang Shen, Ping Li, Min-Bin Chen, Li-Na Zhou","doi":"10.3138/cim-2024-0262","DOIUrl":"https://doi.org/10.3138/cim-2024-0262","url":null,"abstract":"<p><strong>Background: </strong>Radiation-induced lung injury (RILI) is a significant adverse effect of thoracic radiotherapy, potentially impacting patient prognosis. The risk factors for acute radiation pneumonitis (RP) have not been fully clarified. The present study evaluated the predictive value of serum interleukins (ILs) in the occurrence of RP and overall survival in patients with thoracic cancers.</p><p><strong>Methods: </strong>This single-centre retrospective observational study enrolled 435 thoracic cancer patients who underwent chest radiation therapy. Serum levels of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17, TNF-α, IFN-γ, IFN-α were measured by cytometric bead array before radiotherapy. The relationship between clinical characteristics, serum IL levels and the occurrence of RP were analyzed. Cox regression and Kaplan-Meier methods were also performed to investigate the prognostic role of serum IL levels in these patients.</p><p><strong>Results: </strong>The incidence of RP in these patients was 17.01%. Elevated serum levels of IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, TNF-α, IFN-α were all associated with the occurrence of RP. High levels of IL-1β, IL-4, and IL-12p70 were correlated with more severe pneumonitis. Univariate and multivariate logistic regression analysis identified serum IL-6 level as an independent prognostic factor in patients receiving thoracic radiotherapy.</p><p><strong>Conclusions: </strong>Serum interleukin levels are linked to the development of acute RP in patients receiving thoracic radiotherapy. Serum IL-6 could serve as a valuable biomarker in identifying patients at high risk for RP, potentially guiding individualized therapeutic strategies and improving patient management in radiotherapy. Future research should focus on validating IL-6's role in larger cohorts and exploring its integration into clinical practice for the early prediction of RILI.</p>","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"48 1","pages":"29-38"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of combined screening for GADA, ICA, and IAA in new-onset type 2 diabetes mellitus in adults for early diagnosis of latent autoimmune diabetes.","authors":"Shiqi Sun, Xiaobo Shen, Qi Huang, Jiazhong Sun","doi":"10.3138/cim-2024-0157","DOIUrl":"https://doi.org/10.3138/cim-2024-0157","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the detection rate of latent autoimmune diabetes in adults (LADA) among newly diagnosed type 2 diabetes mellitus (T2DM) patients and analyze their clinical characteristics, with the aim of guiding treatment and improving prognosis.</p><p><strong>Methods: </strong>Glutamic acid decarboxylase autoantibody (GADA), islet cell autoantibody (ICA), insulin autoantibody (IAA), blood pressure, height, weight, and BMI, as well as lipid profiles, fasting plasma glucose, insulin, C-peptide, HbA_1c, thyroid-stimulating hormone (TSH), thyroglobulin antibody (TGAb), and thyroid peroxidase antibody (TPOAb) levels were measured in 352 newly diagnosed T2DM patients with disease duration of less than 1 year and non-ketotic onset. The incidence of LADA was calculated, and clinical characteristics of these patients were compared with those of T2DM patients without autoantibodies.</p><p><strong>Results: </strong>The detection rate of LADA was 7.10%. Among the 25 LADA patients, 22 had GADA, 5 had ICA, 2 had IAA, and 2 were positive for all three antibodies. The percentages of GADA, ICA, and IAA among LADA patients were 88.00%, 20.00%, and 8.00%, respectively. Compared with the autoantibody-negative group, LADA patients had lower BMIs and total cholesterol, lower insulin and C-peptide levels, higher neutrophil counts, and increased abnormal TSH levels and TPOAb positivity (<i>P</i> < 0.05). LADA patients exhibited reduced CD4⁺ T cell expression levels, which were lower than those in patients without autoantibodies, while LADA patients' CD8⁺ T cell levels were within the normal range and slightly higher than those in patients without autoantibodies, reflecting a lower CD4⁺/CD8⁺ ratio in LADA patients.</p><p><strong>Conclusion: </strong>A proportion of newly diagnosed T2DM patients have LADA. Early screening for GADA, ICA, and IAA should be performed in newly diagnosed T2DM patients with low BMI and poor islet function. LADA patients may have higher expression of inflammatory immune factors, lower total cholesterol, and a lower CD4⁺/CD8⁺ ratio. The decline in [Formula: see text]-cell function in LADA patients may be associated with decreased CD4⁺ T cell expression.</p>","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"48 1","pages":"24-28"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the Canadian Clinician Investigator Trainees' Research Presented at the 2024 CSCI-CITAC Joint Meeting.","authors":"MohdWessam Al Jawhri, Salonee V Patel, Shubham Soni, Richard Huang, Robert X Lao","doi":"10.3138/CIM-47-4-REPORT","DOIUrl":"https://doi.org/10.3138/CIM-47-4-REPORT","url":null,"abstract":"<p><p>The 2024 Annual Joint Meeting (AJM) and Young Investigators' Forum of the Canadian Society for Clinical Investigation (CSCI) and Clinician Investigator Trainee Association of Canada (CITAC) was held on April 11, 2024, in Vancouver, British Columbia. Hosted in collaboration with the University of British Columbia (UBC) and the International Congress on Academic Medicine (ICAM), this meeting marked a significant opportunity for clinician investigator trainees to present their research and connect with national and international peers. The event included 70 trainees, consisting of 58 MD+ (MD/PhD, MD and PhD, MD-MSc, MD and MSc) and 12 Clinician Investigator Program and/or Surgeon-Scientist Training Program trainees. The keynote speaker, Dr. Marco Marra, delivered a presentation titled \"From <i>C. elegans</i> Genetics to Precision Cancer Genomic Medicine, via the Human Genome Project: Reflections on a Collaborative Scientific Journey.\" Dr. Kenneth Rockwood (Dalhousie University) received the CSCI Distinguished Scientist Award, and Dr. Bertrand Routy (Université de Montréal) received the CSCI Joe Doupe Young Investigator Award. Over 60 abstracts were showcased, most of which are summarized in this review, and six were selected for oral presentations. Following the AJM, trainees participated in ICAM's three-day health research program, where three AJM attendees were recognized among the top four presenters and were invited to the 2024 Canada Gairdner Awards Gala, with one of our AJM attendees winning the competition and being named the next Lindau Nobel Laurate Meetings Nominee.</p>","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"47 4","pages":"3-10"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CIM-CITAC Publication Award.","authors":"","doi":"10.3138/CIM-47-4-AWARD","DOIUrl":"https://doi.org/10.3138/CIM-47-4-AWARD","url":null,"abstract":"","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"47 4","pages":"2"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New CIM Editor-in-Chief.","authors":"","doi":"10.3138/CIM-47-4-ANNOUNCEMENT","DOIUrl":"https://doi.org/10.3138/CIM-47-4-ANNOUNCEMENT","url":null,"abstract":"","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"47 4","pages":"1"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of miR-34a on Endothelial Cell Viability and Apoptosis in Ischemic Stroke: Unraveling the <i>MTHFR</i>-Homocysteine Pathway.","authors":"Lina Liang, Xueli Yi, Chunfang Wang, Li Su, Guijiang Wei","doi":"10.3138/cim-2024-2711","DOIUrl":"10.3138/cim-2024-2711","url":null,"abstract":"<p><strong>Introduction: </strong>Ischemic stroke (IS) is a global health concern, often tied to dyslipidemia and vascular endothelial dysfunction. MicroRNA-34a (miR-34a) was reported to be up-regulated in the blood samples of patients with IS, but the specific role of miR-34a and methylenetetrahydrofolate reductase (MTHFR) in IS remains to be elucidated.</p><p><strong>Methods: </strong>We studied 143 subjects: 71 IS patients, and 72 healthy controls. Human umbilical vein endothelial cells (HUVECs) were cultured and transfected with a miR-34a mimic, inhibitor, or negative control. The miR-34a expression in serum and HUVECs was quantified via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Viability and apoptosis of HUVECs were assessed using CCK-8 assay and flow cytometry. The expression levels of bcl-2, bax, cyt-c, cleaved caspase 3, MTHFR, and homocysteine were measured by Western blot or enzyme-linked immunosorbent assay (ELISA). The relationship between miR-34a and MTHFR was verified by luciferase reporter assay. The levels of MTHFR and homocysteine in serum were examined by ELISA.</p><p><strong>Results: </strong>MiR-34a expression was increased in IS patients and inhibited viability of HUVECs while promoting their apoptosis. Overexpression of miR-34a up-regulated pro-apoptotic proteins (bax, cyt-c and cleaved caspase 3) and down-regulated anti-apoptotic protein bcl-2 in HUVECs. MTHFR was identified as the downstream target of miR-34a and its expression was reduced by miR-34a overexpression, while homocysteine levels increased. Consistently, MTHFR levels were lower and homocysteine levels were higher in IS patients compared with controls.</p><p><strong>Discussion: </strong>Our results suggest that up-regulated miR-34a plays a role in the pathogenesis of IS, potentially through inhibiting MTHFR expression and increasing homocysteine in endothelial cells. Therefore, miR-34a might be a therapeutic target for IS.</p>","PeriodicalId":50683,"journal":{"name":"Clinical and Investigative Medicine","volume":"47 3","pages":"27-37"},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}