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Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes 小扁豆(Lens culinaris Medik.)基因组SSR标记的开发及对相关豆类跨种/属转移性的评估
4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-10-23 DOI: 10.17221/57/2023-cjgpb
Melike Bakır, Şehriban Demir, Cebrail Yıldırım, Abdullah Kahraman
{"title":"Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes","authors":"Melike Bakır, Şehriban Demir, Cebrail Yıldırım, Abdullah Kahraman","doi":"10.17221/57/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/57/2023-cjgpb","url":null,"abstract":"Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (<i>Lens culinaris</i> Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition, we tested the transferability of 86 SSR markers, including the 33 newly developed ones, to other legume species. Transferability rates of lentil SSR markers to other species varied between 13.76% (<i>Vicia sativa</i> L.) and 2.58% (<i>Phaseolus vulgaris</i> L. and <i>Trifolium pratense</i> L.). These new SSR markers could be used in further molecular breeding, population genetics, and genetic mapping studies in lentils and other legume species.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135367110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon 利用散装分离分析(BSA-seq)在甜瓜中开发与白粉病抗性相关的pcr标记
4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-10-02 DOI: 10.17221/40/2023-cjgpb
Yawo Mawunyo Nevame Adedze, Xia Lu, Wenyi Fan, Wenting Zhang, Xue Yang, Zhijun Deng, Md. Amirul Alam, Guangli Xu, Lihua Zhang, Wenhu Li
{"title":"Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon","authors":"Yawo Mawunyo Nevame Adedze, Xia Lu, Wenyi Fan, Wenting Zhang, Xue Yang, Zhijun Deng, Md. Amirul Alam, Guangli Xu, Lihua Zhang, Wenhu Li","doi":"10.17221/40/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/40/2023-cjgpb","url":null,"abstract":"Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F<sub>2</sub> segregating population of melons generated from these two parents was inoculated by the PM pathogen. Based on bulk segregant analysis (BSA) using these 100 InDel markers, the powdery mildew resistance was associated with the genomic region <i>LVpm12.1</i> on the melon chromosome 12. This region overlapped the previously described quantitative trait locus (QTL)-hotspot area carrying multiple PM-resistance QTLs. Moreover, conventional QTL mapping analysis was done, which located <i>LVpm12.1</i> in the region between 22.72 and 23.34 Mb, where three highly polymorphic InDel markers MInDel89, MInDel92, and MInDel93 were detected. Therefore, these markers could be used to track this resistance locus in melon while the lines carrying this locus could be employed in PM melon resistance breeding programs after validation tests.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135830741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Verification of resistance loci pyramiding in popular interspecific grape varieties using SSR markers 利用SSR标记验证常见葡萄种间品种的抗性位点金字塔化
4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-10-02 DOI: 10.17221/43/2023-cjgpb
Martin Hádlík, Miroslav Baránek, Ivana Flajšingerová, Kateřina Baránková
{"title":"Verification of resistance loci pyramiding in popular interspecific grape varieties using SSR markers","authors":"Martin Hádlík, Miroslav Baránek, Ivana Flajšingerová, Kateřina Baránková","doi":"10.17221/43/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/43/2023-cjgpb","url":null,"abstract":"Fungal pathogens Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni (downy mildew) and Erysiphe necator Schwein. (powdery mildew) represent the biggest threats for grape growers worldwide. Under suitable conditions, these pathogens can spread very quickly through vineyards and cause significant damage. The most ecological way to reduce the possibility of infection in vineyards is growing interspecific genotypes which are able to suppress these pathogens and stop the spread of infection. With this in mind, 9 international and 11 Czech grapevine genotypes were analysed in order to genotype the resistance loci present in them. As a tool a set of SSR markers linked to known loci of resistance to downy and powdery mildew was used. Namely, presence of four loci responsible for resistance to Plasmopara viticola (Rpv3, Rpv4, Rpv7, and Rpv10) and two for Erysiphe necator (Ren3 and Ren9) loci were analysed with respective SSR markers. By this way the degree of resistance gene pyramiding was newly assessed in all analysed cultivars and their perspectives in grapevine breeding are discussed.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135833642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic diversity among coloured cotton genotypes in relation to their fibre colour and ploidy level based on SSR markers 基于SSR标记的彩色棉基因型间纤维颜色和倍性水平的遗传多样性
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-07-12 DOI: 10.17221/12/2023-cjgpb
Revanasiddayya Revanasiddayya, Jayaprakash Mohan Nidagundi, B. Fakrudin, P. Kuchanur, L. N. Yogeesh, S. Hanchinal, Talagunda Chandrashekar Suma, G. Sunkad, B. Muralidhara, Doddamani Maheshkumar, Channabasava Channabasava, Patil Sudha, Shivamurthy Rashmi, Virupapura Cholaraya Raghavendra
{"title":"Genetic diversity among coloured cotton genotypes in relation to their fibre colour and ploidy level based on SSR markers","authors":"Revanasiddayya Revanasiddayya, Jayaprakash Mohan Nidagundi, B. Fakrudin, P. Kuchanur, L. N. Yogeesh, S. Hanchinal, Talagunda Chandrashekar Suma, G. Sunkad, B. Muralidhara, Doddamani Maheshkumar, Channabasava Channabasava, Patil Sudha, Shivamurthy Rashmi, Virupapura Cholaraya Raghavendra","doi":"10.17221/12/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/12/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45962210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genotypic and environmental variability and stability of seed yield, oil content and fatty acids in high-oleic and high-linoleic safflower (Carthamus tinctorius L.) lines and cultivars 高油酸和高亚油酸红花品系和品种籽粒产量、含油量和脂肪酸的基因型和环境变异及稳定性
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-06-19 DOI: 10.17221/113/2022-cjgpb
S. Erbaş, H. Baydar, H. Hatipoğlu, H. Koc, M. Babaoğlu, A. Köse
{"title":"Genotypic and environmental variability and stability of seed yield, oil content and fatty acids in high-oleic and high-linoleic safflower (Carthamus tinctorius L.) lines and cultivars","authors":"S. Erbaş, H. Baydar, H. Hatipoğlu, H. Koc, M. Babaoğlu, A. Köse","doi":"10.17221/113/2022-cjgpb","DOIUrl":"https://doi.org/10.17221/113/2022-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46500540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli 西兰花体细胞胚源植物直接体细胞胚发生的诱导及其遗传稳定性
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-06-19 DOI: 10.17221/26/2023-cjgpb
S. Pavlović, J. Damnjanovic, Z. Girek, Lela Belić, M. Ugrinović
{"title":"Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli","authors":"S. Pavlović, J. Damnjanovic, Z. Girek, Lela Belić, M. Ugrinović","doi":"10.17221/26/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/26/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43762891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe disease 青科(Hordeum vulgare L. var. nudum Hook) HvWRKY1基因的鉴定与功能分析。f)叶条病
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-06-15 DOI: 10.17221/14/2023-cjgpb
Gang Jing, Youhua Yao, Li-Gang An, Yongmei Cui, Yixiong Bai, Xin Li, X. Yao, Kunlun Wu
{"title":"Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe disease","authors":"Gang Jing, Youhua Yao, Li-Gang An, Yongmei Cui, Yixiong Bai, Xin Li, X. Yao, Kunlun Wu","doi":"10.17221/14/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/14/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41747722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection of suitable reference genes in Paulownia fortunei (Seem.) Hemsl. under different tissues and abiotic stresses for qPCR normalization 泡桐适宜内参基因的选择Hemsl。在不同组织和非生物胁迫下进行qPCR归一化
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-06-09 DOI: 10.17221/72/2022-cjgpb
Jiang Su, Kanghua Xian, C. Fu, Jinxiang He, Baojun Liu, N. Huang
{"title":"Selection of suitable reference genes in Paulownia fortunei (Seem.) Hemsl. under different tissues and abiotic stresses for qPCR normalization","authors":"Jiang Su, Kanghua Xian, C. Fu, Jinxiang He, Baojun Liu, N. Huang","doi":"10.17221/72/2022-cjgpb","DOIUrl":"https://doi.org/10.17221/72/2022-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49215310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials 黄淮盆地小麦品种幼苗抗叶锈病基因鉴定及田间缓锈试验观察
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-06-06 DOI: 10.17221/9/2023-cjgpb
Huali Yang, Wenda Diao, Xiaocui Yan, T. Gebrewahid, Zaifeng Li, Zhanjun Yao
{"title":"Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials","authors":"Huali Yang, Wenda Diao, Xiaocui Yan, T. Gebrewahid, Zaifeng Li, Zhanjun Yao","doi":"10.17221/9/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/9/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41375529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Crossing possibility for breeding promising orange-fleshed sweetpotato genotypes in Benin 在贝宁培育有前途的橙肉甘薯基因型的杂交可能性
IF 0.9 4区 农林科学
Czech Journal of Genetics and Plant Breeding Pub Date : 2023-06-02 DOI: 10.17221/104/2022-cjgpb
Fernand S. Sohindji, Florent J.-B. Quenum, Nicodème V. Fassinou-Hotegni, Adechina Adékounlé Oke, C. Adje, E. Achigan-Dako
{"title":"Crossing possibility for breeding promising orange-fleshed sweetpotato genotypes in Benin","authors":"Fernand S. Sohindji, Florent J.-B. Quenum, Nicodème V. Fassinou-Hotegni, Adechina Adékounlé Oke, C. Adje, E. Achigan-Dako","doi":"10.17221/104/2022-cjgpb","DOIUrl":"https://doi.org/10.17221/104/2022-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44561159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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