Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Editorial: Chlamydia-host interaction and its pathogenic mechanism","authors":"Zhou Zhou, Yuanjun Liu, Chunfu Yang, Héctor Alex Saka","doi":"10.3389/fcimb.2024.1372714","DOIUrl":"https://doi.org/10.3389/fcimb.2024.1372714","url":null,"abstract":"","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"85 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139860149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Bunyaviruses - threats to health and economy","authors":"Wei Ye, Feihu Yan","doi":"10.3389/fcimb.2024.1369530","DOIUrl":"https://doi.org/10.3389/fcimb.2024.1369530","url":null,"abstract":"","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"38 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139811607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Bunyaviruses - threats to health and economy","authors":"Wei Ye, Feihu Yan","doi":"10.3389/fcimb.2024.1369530","DOIUrl":"https://doi.org/10.3389/fcimb.2024.1369530","url":null,"abstract":"","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"37 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139871803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Molecular pathogenesis and control of viral infectious diseases in children","authors":"Jiali Cao, Chenguang Shen, Wanting He","doi":"10.3389/fcimb.2024.1368324","DOIUrl":"https://doi.org/10.3389/fcimb.2024.1368324","url":null,"abstract":"","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"39 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139810306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Molecular pathogenesis and control of viral infectious diseases in children","authors":"Jiali Cao, Chenguang Shen, Wanting He","doi":"10.3389/fcimb.2024.1368324","DOIUrl":"https://doi.org/10.3389/fcimb.2024.1368324","url":null,"abstract":"","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"6 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139870354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finding Correlations Between mRNA and Protein Levels in <i>Leishmania</i> Development: Is There a Discrepancy?","authors":"Leonardo Cortazzo da Silva,&nbsp;Juliana Ide Aoki,&nbsp;Lucile Maria Floeter-Winter","doi":"10.3389/fcimb.2022.852902","DOIUrl":"https://doi.org/10.3389/fcimb.2022.852902","url":null,"abstract":"<p><p>Multiple genes and proteins have been identified as differentially expressed in the stages of the <i>Leishmania</i> life cycle. The differentiation processes are implicated in specific transcriptional and proteomic adjustments driven by gene expression regulation mechanisms. <i>Leishmania</i> parasites lack gene-specific transcriptional control, and gene expression regulation mostly depends on posttranscriptional mechanisms. Due to the lack of transcriptional regulation, criticism regarding the relevance of transcript quantification as a possible and efficient prediction of protein levels is recurrent in studies that use transcriptomic information. The advent of high-throughput technologies has improved the analysis of genomes, transcriptomes and proteomes for different organisms under several conditions. Nevertheless, defining the correlation between transcriptional and proteomic profiles requires arduous and expensive work and remains a challenge in <i>Leishmania</i>. In this review, we analyze transcriptomic and proteomic data for several <i>Leishmania</i> species in two different stages of the parasite life cycle: metacyclogenesis and amastigogenesis (amastigote differentiation). We found a correlation between mRNA and protein levels of 60.9% and 69.8% for metacyclogenesis and amastigogenesis, respectively; showing that majority mRNA and protein levels increase or decrease concomitantly. Among the analyzed genes that did not present correlation indicate that transcriptomic data should be carefully interpreted as protein expression. We also discuss possible explanations and mechanisms involved for this lack of correlation.</p>","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":" ","pages":"852902"},"PeriodicalIF":5.7,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40641172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and Variation of Serotypes and Pneumococcal Surface Protein A Clades of <i>Streptococcus pneumoniae</i> Strains Isolated From Adult Patients With Invasive Pneumococcal Disease in Japan.","authors":"Bin Chang,&nbsp;Yuki Kinjo,&nbsp;Masatomo Morita,&nbsp;Kosuke Tamura,&nbsp;Hiroshi Watanabe,&nbsp;Yoshinari Tanabe,&nbsp;Koji Kuronuma,&nbsp;Jiro Fujita,&nbsp;Kengo Oshima,&nbsp;Takaya Maruyama,&nbsp;Shuichi Abe,&nbsp;Kei Kasahara,&nbsp;Junichiro Nishi,&nbsp;Tetsuya Kubota,&nbsp;Makoto Ohnishi,&nbsp;Shigeru Suga,&nbsp;Kazunori Oishi","doi":"10.3389/fcimb.2021.617573","DOIUrl":"https://doi.org/10.3389/fcimb.2021.617573","url":null,"abstract":"<p><p>Pneumococcal surface protein A (PspA) is a surface protein of <i>Streptococcus pneumoniae</i> that may be a candidate antigen for new pneumococcal vaccines. This study investigates the distribution of PspA clades of the causative strains of adult invasive pneumococcal disease (IPD) in Japan. Of the 1,939 strains isolated from cases of adult IPD during 2014-2019, the PspA clades of 1,932 (99.6%) strains were determined, and no <i>pspA</i> was detected in the remaining 7 strains (0.4%). PspA clades 1-6 were detected in 786 (40.5%), 291 (15.0%), 443 (22.8%), 369 (19.0%), 33 (1.7%), and 6 (0.3%) strains, respectively. New PspA clades (0.2%) were identified in two non-typeable and two serotype 35B pneumococci. The proportions of clade 1 and clade 2 showed significantly decreased and increased trends, respectively. Furthermore, the PspA clade of pneumococcal strains was partially serotype- and sequence type-dependent. The majority of strains belonging to serotypes contained in both the 13-valent pneumococcal conjugate vaccine (PCV13) and the 23-valent pneumococcal polysaccharide vaccine (PPSV23) belonged to PspA clades 1 or 3. In contrast, the distribution of clades in non-vaccine serotypes was wider than that of vaccine serotype pneumococci. Our findings demonstrate that almost all pneumococcal strains from adult IPD express PspA clades 1-4, especially for non-vaccine serotypes. These results may be useful for the development of a new pneumococcal vaccine with PspA.</p>","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":" ","pages":"617573"},"PeriodicalIF":5.7,"publicationDate":"2021-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38806435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-Derived Mesenchymal Stem Cells Ameliorating <i>Pseudomonas aeruginosa</i>-induced Acute Lung Infection <i>via</i> Inhibition of NLRC4 Inflammasome.","authors":"Lu-Lu Li,&nbsp;Ying-Gang Zhu,&nbsp;Xin-Ming Jia,&nbsp;Dong Liu,&nbsp;Jie-Ming Qu","doi":"10.3389/fcimb.2020.581535","DOIUrl":"https://doi.org/10.3389/fcimb.2020.581535","url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> (PA) is one of the most common Gram-negative bacteria causing hospital-acquired pulmonary infection, with high drug resistance and mortality. Therefore, it is urgent to introduce new non-antibiotic treatment strategies. Mesenchymal stem cells (MSCs), as important members of the stem cell family, were demonstrated to alleviate pathological damage in acute lung injury. However, the potential mechanism how MSC alleviate acute lung infection caused by PA remains unclear.</p><p><strong>Objective: </strong>The purpose of this study was to investigate the effects of Adipose-derived mesenchymal stem cells (ASCs) on acute pulmonary infections and the possible mechanisms how ASCs reduce pulmonary inflammation induced by PA.</p><p><strong>Methods: </strong>The therapeutic and mechanistic effects of ASCs on PA pulmonary infection were evaluated respectively in a murine model as well as in an <i>in vitro</i> model stimulated by PA and co-cultured with ASCs.</p><p><strong>Results: </strong>1. ASCs treatment significantly reduced the bacterial load, inflammation of lung tissue and histopathological damage by PA. 2. PA infection mainly activated Nod-like receptor containing a caspase activating and recruitment domain 4 (NLRC4) inflammasome in the lung of mice. ASCs attenuated acute lung infection in mice by inhibiting NLRC4 inflammasome activation. 3. NLRC4^-/- mice showed a significant improvement in survival rate and lung bacterial load after PA infection. 4. ASCs mainly increased expression and secretion of STC-1 in response to PA-stimulated NLRC4 inflammasome activation.</p><p><strong>Conclusions: </strong>PA infection attenuated macrophage phagocytosis through activation of NLRC4 inflammasome in macrophages, which eventually led to pulmonary inflammatory damage in mouse; ASCs reduced the activation of NLRC4 inflammasome in macrophages induced by PA infection, thereby increasing the phagocytic ability of macrophages, and ultimately improving lung tissue damage in mouse; ASCs may inhibit NLRC4 inflammasome through the secretion of STC-1.</p>","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":" ","pages":"581535"},"PeriodicalIF":5.7,"publicationDate":"2021-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38855192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Gut Microbiota Between <i>Pulsatilla</i> Decoction and Levofloxacin Hydrochloride Therapy on <i>Escherichia coli</i> Infection.","authors":"Xiaoye Liu,&nbsp;Shangwen He,&nbsp;Qiuyue Li,&nbsp;Xiang Mu,&nbsp;Ge Hu,&nbsp;Hong Dong","doi":"10.3389/fcimb.2020.00319","DOIUrl":"https://doi.org/10.3389/fcimb.2020.00319","url":null,"abstract":"<p><p>Gut microbiota serves as a critical indicator for gut health during treatment of pathogenic bacterial infection. Both <i>Pulsatilla</i> Decoction (abbreviated to PD, a traditional Chinese medicine compound) and Levofloxacin Hydrochloride (LVX) were known to have therapeutic effects to intestinal infectious disease. However, the changes of gut microbiota after PD or LVX treatment remain unclear. Herein, this work aimed to investigate the changes of intestinal flora after PD or LVX therapy of <i>Escherichia coli</i> infection in rats. Results revealed that PD exhibited a valid therapeutic approach for <i>E. coli</i> infection via the intestinal protection, as well as the inhibited release of IL-8 and ICAM-1. Besides, PD was beneficial to rebuild the gut microbiota via restoring <i>Bacteroidetes</i> spp in the composition of the gut microbiota. Comparatively, LVX treatment promoted the infection and ravaged gut microbiota by significantly decreasing Bacteroidetes and increasing Firmicutes. These findings not only highlight the mechanism of Chinese herbal formula, but extend the application of PD as veterinary medicine, feed additive and pre-mixing agent for improving the production of animal derived foods.</p>","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":" ","pages":"319"},"PeriodicalIF":5.7,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3389/fcimb.2020.00319","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38195248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Metagenomic Profiling, Urine Metabolomics, and Human Marker Gene Expression as an Integrated Approach to Study Alopecia Areata.","authors":"Daniela Pinto,&nbsp;Francesco Maria Calabrese,&nbsp;Maria De Angelis,&nbsp;Giuseppe Celano,&nbsp;Giammaria Giuliani,&nbsp;Marco Gobbetti,&nbsp;Fabio Rinaldi","doi":"10.3389/fcimb.2020.00146","DOIUrl":"https://doi.org/10.3389/fcimb.2020.00146","url":null,"abstract":"Involvement of the microbiome in many different scalp conditions has been investigated over the years. Studies on the role of the scalp microbiome in specific diseases, such as those involving hair growth alterations like non-cicatricial [androgenetic alopecia (AGA), alopecia areata (AA)] and cicatricial alopecia lichen planopilaris, are of major importance. In the present work, we highlighted the differences in microbial populations inhabiting the scalp of AA subjects and a healthy sample cohort by using an integrated approach relying on metagenomic targeted 16S sequencing analysis, urine metabolomics, and human marker gene expression. Significant differences in genera abundances (p < 0.05) were found in the hypodermis and especially the dermis layer. Based on 16S sequencing data, we explored the differences in predicted KEGG pathways and identified some significant differences in predicted pathways related to the AA pathologic condition such as flagellar, assembly, bacterial chemotaxis, mineral absorption, ABC transporters, cellular antigens, glycosaminoglycan degradation, lysosome, sphingolipid metabolism, cell division, protein digestion and absorption, and energy metabolism. All predicted pathways were significantly enhanced in AA samples compared to expression in healthy samples, with the exceptions of mineral absorption, and ABC transporters. We also determined the expression of TNF-α, FAS, KCNA3, NOD-2, and SOD-2 genes and explored the relationships between human gene expression levels and microbiome composition by Pearson's correlation analysis; here, significant correlations both positive (SOD vs. Staphylococcus, Candidatus Aquiluna) and negative (FAS and SOD2 vs. Anaerococcus, Neisseria, and Acinetobacter) were highlighted. Finally, we inspected volatile organic metabolite profiles in urinary samples and detected statistically significant differences (menthol, methanethiol, dihydrodehydro-beta-ionone, 2,5-dimethylfuran, 1,2,3,4, tetrahydro-1,5,7-trimethylnapthalene) when comparing AA and healthy subject groups. This multiple comparison approach highlighted potential traits associated with AA and their relationship with the microbiota inhabiting the scalp, opening up novel therapeutic interventions in such kind of hair growth disorders mainly by means of prebiotics, probiotics, and postbiotics.","PeriodicalId":505894,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":" ","pages":"146"},"PeriodicalIF":5.7,"publicationDate":"2020-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3389/fcimb.2020.00146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37937992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}