Ain Shams Dental Journal最新文献

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Erythropoietin Gel as an adjunct to Xenograft in the Surgical Management of Intrabony Periodontal Defects :A Randomized Controlled Clinical Study 红细胞生成素凝胶辅助异种移植治疗骨内牙周缺损:一项随机对照临床研究
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.285037.1260
Roaa Mostafa Esmail, Ahmed Elsayed Hamed Amr, Mahmoud Hassan Moussa, Yasmine Ahmed Fouad
{"title":"Erythropoietin Gel as an adjunct to Xenograft in the Surgical Management of Intrabony Periodontal Defects :A Randomized Controlled Clinical Study","authors":"Roaa Mostafa Esmail, Ahmed Elsayed Hamed Amr, Mahmoud Hassan Moussa, Yasmine Ahmed Fouad","doi":"10.21608/asdj.2024.285037.1260","DOIUrl":"https://doi.org/10.21608/asdj.2024.285037.1260","url":null,"abstract":"Aim: The present trial aimed to compare the clinical as well as the radiographic efficacy of Erythropoietin (EPO) gel as an adjunct to xenograft versus xenograft alone in treating intrabony defects. materials and Methods: This research was conducted at the Faculty of Dentistry, Ain Shams University, in the Department of Oral Medicine, Periodontology, and Oral Diagnosis. It was a randomized, double-blind, and controlled study. Twenty-six (stage III) periodontitis patients, with (three-wall) intrabony defects, were randomly divided (Thirteen in each group) into test (EPO gel + xenograft) and control (xenograft alone) groups. The primary outcome was evaluating the changes in the periodontal parameters; the plaque index (PI), sulcular bleeding index (SBI), probing depth (PD), and clinical attachment level (CAL) and wound healing by the Early Healing Index (EHI). The secondary outcome was evaluating the changes in the radiographic parameters including the defect fill (DF) and alveolar crest changes. Results: After the first and second weeks, the test group demonstrated a significant EHI reduction than the control group. At six months, there was a significant gain in CAL and DF in both groups with a significantly lower CAL gain and DF in the test group compared to the control group. There was a significant PD reduction with no significant difference between both groups. Conclusion: EPO gel as an adjunct to xenograft may accelerate soft tissue healing after surgical management of intrabony defects. However, it does not enhance the xenograft regenerative outcomes.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"68 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Evaluation of the Effect of two Resilient Attachments Retaining Mandibular Over-Denture Supported by Implant and Natural Tooth on the Supporting Structures 由种植体和天然牙支持的两种弹性附着体固位下颌覆盖义齿对支持结构影响的临床评估
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.287725.1284
Abdel Rahman M Maged, Noha Helmy Hassan Nawar, Hany Eid, Yasser M. Shawky
{"title":"Clinical Evaluation of the Effect of two Resilient Attachments Retaining Mandibular Over-Denture Supported by Implant and Natural Tooth on the Supporting Structures","authors":"Abdel Rahman M Maged, Noha Helmy Hassan Nawar, Hany Eid, Yasser M. Shawky","doi":"10.21608/asdj.2024.287725.1284","DOIUrl":"https://doi.org/10.21608/asdj.2024.287725.1284","url":null,"abstract":"Aim: This study aimed to assess the effect of two resilient attachments used for retention of mandibular over-denture supported by an implant and a natural tooth on the supporting structures throughout a one-year follow-up period. Materials and methods: Fourteen patients with maxillary completely edentulous arches and only one standing natural mandibular canine were selected. Each patient received a dental implant with 10.5 mm length and 3.8 mm diameter at the canine region of the contralateral side of the mandible. The patients were divided into two groups according to the type of attachment used (n=7); BC group included patients who received mandibular over-dentures retained by bar clip attachment supported by an implant on one side and a natural tooth on the other side. BS group involved patients who received a mandibular implant splinted to the natural canine by bar joint attachment utilizing soft liner material. The patients of both groups were rehabilitated with conventional maxillary complete dentures. Clinical and radiographic assessment were performed immediately after over-denture delivery, six and twelve months from denture insertion. Clinical evaluation involved gingival index. Radiographic evaluation included measuring mesial, distal, buccal and lingual bone height differences. Results: BC group recorded significantly higher bone loss compared to BS group around both dental implants and natural teeth. Conclusion: Considering the limitations of this study, it could be concluded that soft liner retained over-dentures are more effective in decreasing bone resorption around the implants and natural teeth compared to clip retained over-dentures.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"5 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical and retention assessment of soft Liner and O-ring attachment in single implant assisted mandibular overdenture: Randomized clinical Trail. 在单种植体辅助下颌覆盖义齿中使用软衬垫和O形环连接体的临床和固位评估:随机临床试验。
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.275147.1232
Doaa Mohammed Elgendy, Attiah Ali Elgendy, Azza A. El-Segai
{"title":"Clinical and retention assessment of soft Liner and O-ring attachment in single implant assisted mandibular overdenture: Randomized clinical Trail.","authors":"Doaa Mohammed Elgendy, Attiah Ali Elgendy, Azza A. El-Segai","doi":"10.21608/asdj.2024.275147.1232","DOIUrl":"https://doi.org/10.21608/asdj.2024.275147.1232","url":null,"abstract":"","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141277581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Cross-Sectional Study on the Impact of Children's Intelligence Quotient on Their Behavior and Anxiety in a Dental Setting 儿童智商对其在牙科环境中的行为和焦虑的影响的横断面研究
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.275306.1224
Radwa H. AbdelRazek, Dina hamdy Abd El Rahman, Reham Mohammed El-Elhossiny Abdelbasir, Amr Abd El-Aziz
{"title":"A Cross-Sectional Study on the Impact of Children's Intelligence Quotient on Their Behavior and Anxiety in a Dental Setting","authors":"Radwa H. AbdelRazek, Dina hamdy Abd El Rahman, Reham Mohammed El-Elhossiny Abdelbasir, Amr Abd El-Aziz","doi":"10.21608/asdj.2024.275306.1224","DOIUrl":"https://doi.org/10.21608/asdj.2024.275306.1224","url":null,"abstract":"Aim: This study examined the relationship between children's intelligence quotient (IQ) and their levels of dental fear and anxiety (DFA) during their first dental visit. It also evaluated the influence of parental general anxiety on children's anxiety during this visit. Materials and methods: Eighty-eight children, aged 6–12 years, who had never visited a dentist before were conveniently recruited. Recruited children were subjected to the Wechsler Intelligence Scale for Children III (WISC III) and the Children’s Fear Survey Schedule-Dental Subscale (CFSS-DS) to examine their IQ and dental fear and anxiety, respectively. Concerning parental general anxiety, General Anxiety Disorder-7 (GAD-7) was utilized. Children’s behavior at their first dental visit was assessed utilizing Frankl's behavior rating scale. The Arabic-validated versions of WISC III, CFSS-DS, and GAD-7 were used. The correlations were examined using Spearman's rank-order correlation coefficient (P<0.05). Results: There was a negative correlation between children’s IQ score and their DFA levels. There was a positive association between children’s DFA and their parent’s general anxiety levels. There was a favorable positive correlation between the degree of cooperation and the children’s IQ scores during their first dental visit. Conclusion: This study reveals the common occurrence of fear and anxiety in children during dental visits between the ages of 6 and 12. The findings highlight the complex interplay between cognitive abilities, parental influence, and dental experiences in children. It emphasizes the importance of addressing both child and parental anxiety to promote positive dental visits.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"57 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141275817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential Effect of Thymoquinone on HNO-97 Cell Line 胸腺醌对 HNO-97 细胞株的潜在影响
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.274346.1222
D. Eloraby, S. Elgayar, Marwa Mokbel, Amr El-Bolok
{"title":"Potential Effect of Thymoquinone on HNO-97 Cell Line","authors":"D. Eloraby, S. Elgayar, Marwa Mokbel, Amr El-Bolok","doi":"10.21608/asdj.2024.274346.1222","DOIUrl":"https://doi.org/10.21608/asdj.2024.274346.1222","url":null,"abstract":"Aim : the aim of this the study was to evaluate the histopathological changes of the buccal mucosa in the advanced age of albino rats and linking these changes with increased tissue cancer tendency. Materials and methods: fourteen adult male albino rats were randomly divided into two equal groups as follow: Group 1: 6 months old group. Group 2: 24 months old group. all animals were euthanized and buccal mucosa specimen were processed for hematoxylin and eosin staining and immunohistochemically prepared for e-cadherin gene expression. Results : histological examination of buccal mucosal tissues (epithelium and lamina propria) of group 2 showed significant atrophic changes and sort of loss of the tissue integrity as compared to group I . Significant downregulation of e -cadherin gene expression was also noticed in the aged group. Conclusion : the present study concluded that oral tissues demonstrate progressive decline in their functional properties as a result of changes related to aging.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"1 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141277813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial behavior of calcium fluoride-modified tricalcium silicate cement against different oral bacterial strains 氟化钙改性硅酸三钙水泥对不同口腔细菌菌株的抗菌行为
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.293174.1299
Ahmed Elbanna, M. Elmesellawy, D. A. El-Refai
{"title":"Antibacterial behavior of calcium fluoride-modified tricalcium silicate cement against different oral bacterial strains","authors":"Ahmed Elbanna, M. Elmesellawy, D. A. El-Refai","doi":"10.21608/asdj.2024.293174.1299","DOIUrl":"https://doi.org/10.21608/asdj.2024.293174.1299","url":null,"abstract":"Aim: This study aimed to evaluate the effect of adding CaF 2 to Biodentine on its antibacterial behavior against Streptococcus mutans , Lactobacillus acidophilus , and Enterococcus faecalis strains and the fluoride release. Materials and methods: The study included three main groups: unmodified Biodentine, 5% wt CaF 2 -modified Biodentine , and 10% wt CaF 2 -modified Biodentine . The antibacterial activity of the tested cement was evaluated against the three bacterial strains through direct contact and agar diffusion tests. Fluoride release in deionized water at different intervals (1 day, one week, and one month) was assessed using ICP-MS. Results: The direct contact test revealed that the different experimental biodentine groups had lower optical density than the bacteria groups after one day, which was confirmed at seven days. The 10% group showed a significantly higher antibacterial effect against S. mutans and L. acidophilus than the other biodentine groups. The agar diffusion test showed significant differences between groups regarding Streptococcus mutans and Lactobacillus acidophilus . The positive control group had significantly higher inhibition zone values than the 5% CaF 2 -modified and unmodified Biodentine groups. The 10% CaF 2 -modified group showed no statistically significant difference compared to the positive control. For Enterococcus faecalis , the positive control exhibited a significantly higher value than other groups. None of the experimental groups showed inhibition zones against Enterococcus faecalis . Conclusions: Adding 10% CaF 2 to Biodentine could improve its antibacterial effect against S. mutans and L. acidophilus. However, its effectiveness against Enterococcus faecalis is questionable. The addition of 10% CaF2 also offers fluoride-releasing qualities to Biodentine.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"60 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141281009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of surface roughness and fracture resistance of maxillary conventional & digital complete dentures: An Invitro study 上颌传统全口义齿和数码全口义齿表面粗糙度和抗折断性的评估:一项特邀研究
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.277269.1231
Eman Gamal Abdelghaffar
{"title":"Evaluation of surface roughness and fracture resistance of maxillary conventional & digital complete dentures: An Invitro study","authors":"Eman Gamal Abdelghaffar","doi":"10.21608/asdj.2024.277269.1231","DOIUrl":"https://doi.org/10.21608/asdj.2024.277269.1231","url":null,"abstract":"Aim: to evaluate the surface roughness and fracture resistance of complete dentures made using heat-cured polymethyl methacrylate (PMMA) resin manufactured by conventional technique & digital complete dentures manufactured by additive manufacturing technique. Materials and Methods : A total of 24 complete dentures were made (n=24), 12 dentures were made using heat-cured polymethyl methacrylate (PMMA) resin manufactured by the conventional methods. 12 dentures were designed by Exocad software program & constructed using additive manufacturing technique. Surface roughness was evaluated using optical profilometry. Fracture resistance was evaluated using universal testing machine. Data were analysed using unpaired t-test where α = 0.05. Results: There was no statistically significant difference found between the two groups when comparing the surface roughness values (P=0.5137). Conventional PMMA dentures showed mean (2.52± 0.14 µm), while 3D-printed dentures showed mean (2.59± 0.38 µm). However, regarding the fracture resistance, there was statistically significant difference found between the two groups (P<0.5), the conventional PMMA dentures showed higher mean (572.49± 13.07 N), while the digital dentures showed lesser mean with (202.51 ± 10.35 N). Conclusion : Conventional PMMA dentures and 3D-printed dentures had similar surface roughness. However conventional PMMA dentures had better fracture resistance when compared to 3D-printed dentures.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"73 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Different Post and Core Systems for Restoring Pulpectomized Primary Incisors: An In-Vitro Study 评估用于修复已切除牙髓的原切牙的不同桩核系统:体外研究
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.271877.1218
Mohamed Elfatih Mansour, Ahmed Hamdy Wahba, Abeer Abdellatif, I. Elkalla
{"title":"Evaluation of Different Post and Core Systems for Restoring Pulpectomized Primary Incisors: An In-Vitro Study","authors":"Mohamed Elfatih Mansour, Ahmed Hamdy Wahba, Abeer Abdellatif, I. Elkalla","doi":"10.21608/asdj.2024.271877.1218","DOIUrl":"https://doi.org/10.21608/asdj.2024.271877.1218","url":null,"abstract":"Aim : This in-vitro study was conducted to assess the fracture resistance (FR) of different post and core systems for restoring pulpectomized primary incisors. Materials and methods : Forty extracted primary incisors were collected, prepared, and then obturated using Metapex. Teeth were allocated into four groups. Group (A): teeth restored with human dentin posts; Group (B): bovine dentin posts; Group (C): glass fiber posts; and Group (D): orthodontic stainless-steel wire in omega-form posts. Dentin blocks were obtained from the roots of extracted human maxillary premolars and bovine permanent incisors to fabricate the dentin posts. These blocks were formed into post shapes using a Computer Numerical Control (CNC) machine. The posts underwent cementation using glass ionomer cement, followed by coronal buildup with composite. After thermocycling, the FR and mode of failure were examined. The FR was analyzed using the Kruskal-Wallis test, while the mode of failure was analyzed using the Monte Carlo test. P-values ≤ 0.05 were used to determine statistical significance. Results : The median maximum compressive load in group (A) was 386.87 N, in group (B) it was 379.27 N, in group (C) it was 522.16 N, and in group (D) it was 469.14 N. The differences between the groups were not statistically significant regarding the maximum compressive load and the failure mode. Conclusion : Glass fiber posts and omega posts had higher FR compared to dentin posts, although the four groups can be used effectively for restoring pulpectomized primary incisors.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"33 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141275505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Dental Stone Fluidity Properties, Compatibility with Impression Materials, and Detail Reproduction after Incorporation of 0.2% Chlorhexidine Solution 加入 0.2% 洗必泰溶液后牙石流动性能、与印模材料的兼容性以及细节再现的影响
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.272800.1219
Mohamed Abdulmunem Abdulateef, Ibrahim H. Alfahdawi, Anes Adnan Yaseen
{"title":"Effect of Dental Stone Fluidity Properties, Compatibility with Impression Materials, and Detail Reproduction after Incorporation of 0.2% Chlorhexidine Solution","authors":"Mohamed Abdulmunem Abdulateef, Ibrahim H. Alfahdawi, Anes Adnan Yaseen","doi":"10.21608/asdj.2024.272800.1219","DOIUrl":"https://doi.org/10.21608/asdj.2024.272800.1219","url":null,"abstract":"Aim: Evaluate the effect of incorporation of 0.2% CHX solution on the detail reproduction compatibility with impression materials, and fluidity of Iraqi stone comparing with Hi-stone. Materials and Methods: W/P ratio 33ml/100g for Hi-stone and 38ml/100g for Iraqi stone. For detail reproduction detail test block which had 60-degree angle grooves width (0.025, 0.050, 0.075, 0.1, 0.2, 0.3) mm with cross line. Silicone, alginate, and zinc oxide eugenol (ZOE) used for compatibility with impression materials test (n= 10), after setting of impression material, poured with stone and scored. Fluidity was obtained with a different w/p ratio by a slump plate diameter. Results: The mean reproduction of detail was 1.03 ± 0.4 Iraqi stone and 1.03±0.4 Hi-stones. The mean scoring with silicone of Hi-stone was 1.03±0.4 while Iraqi stone was 1.03±0.4. The mean diameter of the slump test was 99.0±1 with Hi-stone, a higher mean of 99.6±1.34 of new Iraqi stone. Conclusion: 0.2% CHX improved the reproduction of detail ability of both types of stone, the same compatibility with silicone and alginate impression materials but less compatibility with ZOE for the two types of stone. Both stones demonstrated reasonable and similar fluidity.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"26 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study. 牙髓再生过程中使用的不同氢氧化钙配方对人牙周韧带干细胞的活力、矿化和牙本质/骨质/骨质分化潜能的影响。体外研究。
Ain Shams Dental Journal Pub Date : 2024-06-01 DOI: 10.21608/asdj.2024.289270.1280
Shehabeldin Saber
{"title":"Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.","authors":"Shehabeldin Saber","doi":"10.21608/asdj.2024.289270.1280","DOIUrl":"https://doi.org/10.21608/asdj.2024.289270.1280","url":null,"abstract":"Aim: To evaluate the effect of CH and NCH on the viability, attachment, osteogenic, cementogenic and dentinogenic differentiation potential of hPDLSCs in-vitro. Materials and Methods: Calcium Hydroxide (CH) and nano calcium hydroxide (NCH) powders were initially characterized using transmission electron microscope (TEM), X-ray Diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and a zeta sizer instrument. Standard dentin discs were prepared, irrigated according to the Regenerative Endodontic Procedures (REP) protocol, then treated with 1 mg/ml of CH or NCH pastes, and incubated for 1 week. Untreated discs served as control. Then, they were washed off, and all discs were treated with 17% EDTA before seeding of human periodontal ligament stem cells (hPDLSCs) . Cell viability was assessed using MTT assay. Mineralized nodules and alkaline phosphatase (ALP) activity were determined to assess cell differentiation. Cell dentino/cementogenic and osteogenic potential were assessed through monitoring gene expression levels Dentin sialophosphoprotein (DSSP), Cementum protein 1 (CEMP1), Runt-related transcription factor 2 (RUNX2), Osteoprotegrin (OG), and Osteocalcin (OC) using RT-qPCR. Results: TEM examination of NCH powder particles revealed a regular pattern and hexagonal shape with side dimension up to 60 ±10 nm. XRD analysis of NCH powder demonstrated its highly crystalline nature. FTIR spectroscopy confirmed that CH and NCH consisted mainly of pure calcium hydroxide. The zeta sizer instrument showed that NCH is positively charged with an average zeta potential of +27.8 mV. hPDLSCs seeded on NCH-treated discs showed significantly higher cell survival , more mineralization, higher alkaline phosphatase activity and increased expression of DSPP, CEMP1, RUNX2, OPG and OC markers. Conclusion: NCH can be an alternative ICM during REP.","PeriodicalId":505319,"journal":{"name":"Ain Shams Dental Journal","volume":"55 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141277268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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