Expert Review of Proteomics最新文献_第5页

Proteomic alterations in patients with atopic dermatitis. 特应性皮炎患者的蛋白质组变化。

IF 3.8 3区生物学

Expert Review of Proteomics Pub Date : 2024-05-01 Epub Date: 2024-05-22 DOI: 10.1080/14789450.2024.2350938

Ashley Obi, Camille Rothenberg-Lausell, Sophia Levit, Ester Del Duca, Emma Guttman-Yassky

{"title":"Proteomic alterations in patients with atopic dermatitis.","authors":"Ashley Obi, Camille Rothenberg-Lausell, Sophia Levit, Ester Del Duca, Emma Guttman-Yassky","doi":"10.1080/14789450.2024.2350938","DOIUrl":"10.1080/14789450.2024.2350938","url":null,"abstract":"Introduction: Atopic Dermatitis (AD) is the most common inflammatory skin disease with a complex and multifactorial pathogenesis. The use of proteomics in understanding AD has yielded the discovery of novel biomarkers and may further expand therapeutic options.Areas covered: This review summarizes the most recent proteomic studies and the methodologies used in AD. It describes novel biomarkers that may monitor disease course and therapeutic response. The review also highlights skin and blood biomarkers characterizing different AD phenotypes and differentiates AD from other inflammatory skin disorders. A literature search was conducted by querying Scopus, Google Scholar, Pubmed/Medline, and Clinicaltrials.gov up to June 2023.Expert opinion: The integration of proteomics into research efforts in atopic dermatitis has broadened our understanding of the molecular profile of AD through the discovery of new biomarkers. In addition, proteomics may contribute to the development of targeted treatments ultimately improving personalized medicine. An increasing number of studies are utilizing proteomics to explore this heterogeneous disease.","PeriodicalId":50463,"journal":{"name":"Expert Review of Proteomics","volume":" ","pages":"247-257"},"PeriodicalIF":3.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid-phase separations coupled with ion mobility-mass spectrometry for next-generation biopharmaceutical analysis. 用于新一代生物制药分析的液相分离与离子迁移质谱联用技术。

IF 3.8 3区生物学

Expert Review of Proteomics Pub Date : 2024-05-01 Epub Date: 2024-07-01 DOI: 10.1080/14789450.2024.2373707

Devin M Makey, Brandon T Ruotolo

{"title":"Liquid-phase separations coupled with ion mobility-mass spectrometry for next-generation biopharmaceutical analysis.","authors":"Devin M Makey, Brandon T Ruotolo","doi":"10.1080/14789450.2024.2373707","DOIUrl":"10.1080/14789450.2024.2373707","url":null,"abstract":"Introduction: The pharmaceutical industry continues to expand its search for innovative biotherapeutics. The comprehensive characterization of such therapeutics requires many analytical techniques to fully evaluate critical quality attributes, making analysis a bottleneck in discovery and development timelines. While thorough characterization is crucial for ensuring the safety and efficacy of biotherapeutics, there is a need to further streamline analytical characterization and expedite the overall timeline from discovery to market.Areas covered: This review focuses on recent developments in liquid-phase separations coupled with ion mobility-mass spectrometry (IM-MS) for the development and characterization of biotherapeutics. We cover uses of IM-MS to improve the characterization of monoclonal antibodies, antibody-drug conjugates, host cell proteins, glycans, and nucleic acids. This discussion is based on an extensive literature search using Web of Science, Google Scholar, and SciFinder.Expert opinion: IM-MS has the potential to enhance the depth and efficiency of biotherapeutic characterization by providing additional insights into conformational changes, post-translational modifications, and impurity profiles. The rapid timescale of IM-MS positions it well to enhance the information content of existing assays through its facile integration with standard liquid-phase separation techniques that are commonly used for biopharmaceutical analysis.","PeriodicalId":50463,"journal":{"name":"Expert Review of Proteomics","volume":" ","pages":"259-270"},"PeriodicalIF":3.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11299228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applicability of selected reaction monitoring for precise screening tests. 选定反应监测在精确筛选测试中的适用性。

IF 3.8 3区生物学

Expert Review of Proteomics Pub Date : 2024-05-01 Epub Date: 2024-05-08 DOI: 10.1080/14789450.2024.2350975

Ahrum Son, Woojin Kim, Wonseok Lee, Jongham Park, Hyunsoo Kim

{"title":"Applicability of selected reaction monitoring for precise screening tests.","authors":"Ahrum Son, Woojin Kim, Wonseok Lee, Jongham Park, Hyunsoo Kim","doi":"10.1080/14789450.2024.2350975","DOIUrl":"10.1080/14789450.2024.2350975","url":null,"abstract":"Introduction: The proactive identification of diseases through screening tests has long been endorsed as a means to preempt symptomatic onset. However, such screening endeavors are fraught with complications, such as diagnostic inaccuracies, procedural risks, and patient unease during examinations. These challenges are amplified when screenings for multiple diseases are administered concurrently. Selected Reaction Monitoring (SRM) offers a unique advantage, allowing for the high-throughput quantification of hundreds of analytes with minimal interferences.Areas covered: Our research posits that SRM-based assays, traditionally tailored for single-disease biomarker profiling, can be repurposed for multi-disease screening. This innovative approach has the potential to substantially alleviate time, labor, and cost demands on healthcare systems and patients alike. Nonetheless, there are formidable methodological hurdles to overcome. These include difficulties in detecting low-abundance proteins and the risk of model overfitting due to the multiple functionalities of single proteins across different disease spectrums - issues especially pertinent in blood-based assays where detection sensitivity is constrained. As we move forward, technological strides in sample preparation, online extraction, throughput, and automation are expected to ameliorate these limitations.Expert opinion: The maturation of mass spectrometry's integration into clinical laboratories appears imminent, positioning it as an invaluable asset for delivering highly sensitive, reproducible, and precise diagnostic results.","PeriodicalId":50463,"journal":{"name":"Expert Review of Proteomics","volume":" ","pages":"237-246"},"PeriodicalIF":3.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the future of oral cancer diagnosis: advances in salivary proteomics 塑造口腔癌诊断的未来：唾液蛋白质组学的进展

IF 3.4 3区生物学

Expert Review of Proteomics Pub Date : 2024-04-16 DOI: 10.1080/14789450.2024.2343585

Oriana Barros, Vito G. D’Agostino, Lucio Lara Santos, Rui Vitorino, Rita Ferreira

引用次数: 0

Revisiting the antigen markers of vector-borne parasitic diseases identified by immunomics: identification and application to disease control 重新审视免疫组学确定的病媒传播寄生虫病抗原标记：确定并应用于疾病控制

IF 3.4 3区生物学

Expert Review of Proteomics Pub Date : 2024-04-08 DOI: 10.1080/14789450.2024.2336994

Xia Zhou, Qianqian Zhang, Jun-Hu Chen, Jian-Feng Dai, Kokouvi Kassegne

引用次数: 0

Proteomic scrutiny of nasal microbiomes: implications for the clinic. 鼻腔微生物组的蛋白质组学检查：对临床的影响。

IF 3.4 3区生物学

Expert Review of Proteomics Pub Date : 2024-04-01 Epub Date: 2024-03-04 DOI: 10.1080/14789450.2024.2323983

Ke Zhu, Yan Jin, Yun Zhao, Andong He, Ran Wang, Chao Cao

{"title":"Proteomic scrutiny of nasal microbiomes: implications for the clinic.","authors":"Ke Zhu, Yan Jin, Yun Zhao, Andong He, Ran Wang, Chao Cao","doi":"10.1080/14789450.2024.2323983","DOIUrl":"10.1080/14789450.2024.2323983","url":null,"abstract":"Introduction: The nasal cavity is the initial site of the human respiratory tract and is one of the habitats where microorganisms colonize. The findings from a growing number of studies have shown that the nasal microbiome is an important factor for human disease and health. 16S rRNA sequencing and metagenomic next-generation sequencing (mNGS) are the most commonly used means of microbiome evaluation. Among them, 16S rRNA sequencing is the primary method used in previous studies of nasal microbiomes. However, neither 16S rRNA sequencing nor mNGS can be used to analyze the genes specifically expressed by nasal microorganisms and their functions. This problem can be addressed by proteomic analysis of the nasal microbiome.Areas covered: In this review, we summarize current advances in research on the nasal microbiome, introduce the methods for proteomic evaluation of the nasal microbiome, and focus on the important roles of proteomic evaluation of the nasal microbiome in the diagnosis and treatment of related diseases.Expert opinion: The detection method for microbiome-expressed proteins is known as metaproteomics. Metaproteomic analysis can help us dig deeper into the nasal microbiomes and provide new targets and ideas for clinical diagnosis and treatment of many nasal dysbiosis-related diseases.","PeriodicalId":50463,"journal":{"name":"Expert Review of Proteomics","volume":" ","pages":"169-179"},"PeriodicalIF":3.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139991724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prioritization of genes for translation: a computational approach. 翻译基因的优先排序：一种计算方法。

IF 3.8 3区生物学

Expert Review of Proteomics Pub Date : 2024-04-01 Epub Date: 2024-04-09 DOI: 10.1080/14789450.2024.2337004

Simone C da Silva Rosa, Amir Barzegar Behrooz, Sofia Guedes, Rui Vitorino, Saeid Ghavami

{"title":"Prioritization of genes for translation: a computational approach.","authors":"Simone C da Silva Rosa, Amir Barzegar Behrooz, Sofia Guedes, Rui Vitorino, Saeid Ghavami","doi":"10.1080/14789450.2024.2337004","DOIUrl":"10.1080/14789450.2024.2337004","url":null,"abstract":"Introduction: Gene identification for genetic diseases is critical for the development of new diagnostic approaches and personalized treatment options. Prioritization of gene translation is an important consideration in the molecular biology field, allowing researchers to focus on the most promising candidates for further investigation.Areas covered: In this paper, we discussed different approaches to prioritize genes for translation, including the use of computational tools and machine learning algorithms, as well as experimental techniques such as knockdown and overexpression studies. We also explored the potential biases and limitations of these approaches and proposed strategies to improve the accuracy and reliability of gene prioritization methods. Although numerous computational methods have been developed for this purpose, there is a need for computational methods that incorporate tissue-specific information to enable more accurate prioritization of candidate genes. Such methods should provide tissue-specific predictions, insights into underlying disease mechanisms, and more accurate prioritization of genes.Expert opinion: Using advanced computational tools and machine learning algorithms to prioritize genes, we can identify potential targets for therapeutic intervention of complex diseases. This represents an up-and-coming method for drug development and personalized medicine.","PeriodicalId":50463,"journal":{"name":"Expert Review of Proteomics","volume":" ","pages":"125-147"},"PeriodicalIF":3.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140337532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomics applications in next generation induced pluripotent stem cell models. 蛋白质组学在新一代诱导多能干细胞模型中的应用。

IF 3.8 3区生物学

Expert Review of Proteomics Pub Date : 2024-04-01 Epub Date: 2024-03-27 DOI: 10.1080/14789450.2024.2334033

Vyshnavi Manda, Jay Pavelka, Edward Lau

引用次数: 0

Bringing proteomics to bear on male fertility: key lessons. 将蛋白质组学应用于男性生育：关键经验。

IF 3.8 3区生物学

Expert Review of Proteomics Pub Date : 2024-04-01 Epub Date: 2024-04-10 DOI: 10.1080/14789450.2024.2327553

Rachel Parkes, Thomas X Garcia

{"title":"Bringing proteomics to bear on male fertility: key lessons.","authors":"Rachel Parkes, Thomas X Garcia","doi":"10.1080/14789450.2024.2327553","DOIUrl":"10.1080/14789450.2024.2327553","url":null,"abstract":"Introduction: Male infertility is a major public health concern globally. Proteomics has revolutionized our comprehension of male fertility by identifying potential infertility biomarkers and reproductive defects. Studies comparing sperm proteome with other male reproductive tissues have the potential to refine fertility diagnostics and guide infertility treatment development.Areas covered: This review encapsulates literature using proteomic approaches to progress male reproductive biology. Our search methodology included systematic searches of databases such as PubMed, Scopus, and Web of Science for articles up to 2023. Keywords used included 'male fertility proteomics,' 'spermatozoa proteome,' 'testis proteomics,' 'epididymal proteomics,' and 'non-hormonal male contraception.' Inclusion criteria were robust experimental design, significant contributions to male fertility, and novel use of proteomic technologies.Expert opinion: Expert analysis shows a shift from traditional research to an integrative approach that clarifies male reproductive health's molecular intricacies. A gap exists between proteomic discoveries and clinical application. The expert opinions consolidated here not only navigate the current findings but also chart the future proteomic applications for scientific and clinical breakthroughs. We underscore the need for continued investment in proteomic research - both in the technological and collaborative arenas - to further unravel the secrets of male fertility, which will be central to resolving fertility issues in the coming era.","PeriodicalId":50463,"journal":{"name":"Expert Review of Proteomics","volume":" ","pages":"181-203"},"PeriodicalIF":3.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11426281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The method developer's guide to oligonucleotide design. 方法开发人员的寡核苷酸设计指南。

IF 3.4 3区生物学

Expert Review of Proteomics Pub Date : 2024-01-01 Epub Date: 2024-02-16 DOI: 10.1080/14789450.2024.2318565

Leonie Wenson, Mattias Leino, Malin Jarvius, Johan Heldin, Björn Koos, Ola Söderberg

引用次数: 0