Folia Biologica-Krakow最新文献_第5页

Evidence for the Existence of Two Prolactin Isoforms in the Developing Pituitary Gland of the Goose (Anser cygnoides) 鹅脑垂体发育中两种催乳素异构体存在的证据

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2022-04-28 DOI: 10.3409/fb_70-1.01

Shenqiang Hu, Li Li, Xufang Ren, Enhua Qing, D. Deng, H. He, Liang Li, Jiwen Wang

{"title":"Evidence for the Existence of Two Prolactin Isoforms in the Developing Pituitary Gland of the Goose (Anser cygnoides)","authors":"Shenqiang Hu, Li Li, Xufang Ren, Enhua Qing, D. Deng, H. He, Liang Li, Jiwen Wang","doi":"10.3409/fb_70-1.01","DOIUrl":"https://doi.org/10.3409/fb_70-1.01","url":null,"abstract":"Compared to Galliformes such as chicken and turkey, very little is known about the existence and expression of isoforms of prolactin (PRL) in the pituitary glands of Anseriformes. In this study, by generating a rabbit-anti-goose (Anser cygnoides) PRL polyclonal\u0000 antibody, we analysed the expression patterns of goose PRL isoforms in the embryonic and post-hatch development of the pituitary gland. Our results showed that two immunoreactive bands with molecular weights of about 23 and 26 kDa were detected using the Western blot technique, corresponding\u0000 to the non-glycosylated (NG-) and the glycosylated (G-) isoform of PRL, respectively. The protein levels of the total PRL in a goose increased gradually from the embryonic day (ED) 22 to the post-hatch day (PD) 28, with a non-significant decrease on PD6. Furthermore, the percentage of G-PRL\u0000 in the pituitary gland of the goose fluctuated from about 30.3% to 54.7% throughout the embryonic and post-hatch development. At the mRNA level, the expression of PRL increased steadily during the development and reached the highest levels on PD12, but later showed a non-significant\u0000 decrease on PD28. The inconsistent expression patterns between the PRL mRNA and protein during the stages from PD6 to PD28 indicated that the PRL gene expression involves both transcriptional and post-translational regulation. Taken together, our data unequivocally demonstrated\u0000 the existence of NG- and G-PRL in the pituitary gland of a goose and that the expression of the total PRL as well as the percentage of G-PRL significantly changed during embryonic and post-hatch development, indicating that the versatile biological functions of PRL during the ontogenesis of\u0000 a goose could be closely related to changes in both its total expression and the degree of glycosylation in the pituitary gland.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44040100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Resting Frequency of Echolocation Calls within a Lesser Horseshoe Bat Population (Southern Poland) and its Relation to Body Size, Condition and Mass 小马蹄蝙蝠种群（波兰南部）回声定位叫声的静息频率及其与体型、状况和质量的关系

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2022-04-28 DOI: 10.3409/fb_70-1.04

Joanna Kohyt, E. Pierzchała, Aleksandra Koza, K. Piksa

{"title":"Resting Frequency of Echolocation Calls within a Lesser Horseshoe Bat Population (Southern Poland) and its Relation to Body Size, Condition and Mass","authors":"Joanna Kohyt, E. Pierzchała, Aleksandra Koza, K. Piksa","doi":"10.3409/fb_70-1.04","DOIUrl":"https://doi.org/10.3409/fb_70-1.04","url":null,"abstract":"The echolocation calls emitted by stationary bats are characterised by their resting frequency (RF). The ecological role of RF has been widely discussed across the literature concerning the Rhinolophidae family, where it has been found that the RF may vary depending on many factors,\u0000 although its role in shaping the variability of different populations remains unclear, and the data for many species – including Rhinolophus hipposideros – is scarce. In this study, we aimed to determine how sex, age and biometric parameters (body mass, forearm length and\u0000 the body condition index) affected the RF in a R. hipposideros population and to investigate the individual variability in this parameter. Bats were captured in front of two Carpathian caves in Southern Poland during the mating season. The echolocation calls of the hand-held bats were\u0000 recorded, and later their peak frequency was measured with computer software. The analyses showed higher RF values for females than for males, but (in contrast to previous reports) no differences between the age classes were identified. RF did not correlate with any biometric parameters in\u0000 the studied population, which rules out the possibility of quality characteristic signalling through this parameter. However, we observed significant individual differences in RF within the sex-age groups, which might reflect some communication potential. The discrepancies among the research\u0000 results available for this topic indicate the need for further studies aimed at investigating the variability of RF and its role across species distribution ranges and their phenology.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41783784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-214-3p Ameliorates LPS-Induced Cardiomyocyte Injury by Inhibiting Cathepsin B. MicroRNA-214-3p通过抑制组织蛋白酶B改善lps诱导的心肌细胞损伤。

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068020078

W Yan, Y Feng, Z Lei, W Kuang, C Long

{"title":"MicroRNA-214-3p Ameliorates LPS-Induced Cardiomyocyte Injury by Inhibiting Cathepsin B.","authors":"W Yan, Y Feng, Z Lei, W Kuang, C Long","doi":"10.14712/fb2022068020078","DOIUrl":"10.14712/fb2022068020078","url":null,"abstract":"Myocardial injury is a common complication of sepsis. MicroRNA (miRNA) miR-214-3p is protective against myocardial injury caused by sepsis, but its mechanism in lipopolysaccharide (LPS)- induced cardiomyocyte injury is still unclear. An AC16 cell injury model was induced by LPS treatment. Cell Counting Kit-8 and flow cytometry assay showed decreased cell viability and increased apoptosis in LPS-treated AC16 cells. The levels of caspase- 3, Bax, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), myosin 6 (Myh6), myosin 7 (Myh7), reactive oxygen species (ROS), and malondialdehyde (MDA) were increased in LPS-treated AC16 cells, but the levels of Bcl-2 and superoxide dismutase (SOD) were decreased. MiR-214-3p was down-regulated and cathepsin B (CTSB) was upregulated in LPS-treated AC16 cells. At the same time, miR-214-3p could target CTSB and reduce its expression. We also found that a miR-214-3p mimic or CTSB silencing could significantly reduce LPSinduced apoptosis, decrease ROS, MDA, caspase-3, and Bax and increase SOD and Bcl-2. CTSB silencing could significantly reduce ANP, BNP, Myh6, and Myh7 in LPS-treated AC16 cells. The effects of CTSB silencing were reversed by a miR-214-3p inhibitor. In summary, miR-214-3p could inhibit LPSinduced myocardial injury by targeting CTSB, which provides a new idea for myocardial damage caused by sepsis.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 2 1","pages":"78-85"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67053242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenols of Antibacterial Potential - May They Help in Resolving Some Present Hurdles in Medicine? 多酚具有抗菌潜力——它们是否有助于解决目前医学上的一些障碍？

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068030087

K Zapletal, G Machnik, B Okopień

引用次数: 0

No Inhibitory Effect of Heparinized Blood on Real-Time PCR Analysis of Thrombophilic Mutations. 肝素化血对实时PCR分析嗜血栓突变无抑制作用。

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068050201

M Beránek, J Hegerová, M Michajlíková, P Dulíček, V Palička

{"title":"No Inhibitory Effect of Heparinized Blood on Real-Time PCR Analysis of Thrombophilic Mutations.","authors":"M Beránek, J Hegerová, M Michajlíková, P Dulíček, V Palička","doi":"10.14712/fb2022068050201","DOIUrl":"10.14712/fb2022068050201","url":null,"abstract":"We compared the efficiency of real-time PCR analysis of FII (c.*97G>A, G20210A) and FV Leiden (c.1601G>A) thrombophilic mutations in the samples obtained from venous blood treated with various anti coagulant agents (EDTA, heparin, and sodium fluoride with potassium oxalate), or from clotted venous blood; one hundred samples of wild-type subjects were tested. Genomic DNA extracts and whole blood specimens modified by 90 °C heating were analysed by real-time PCR analysis; cycle threshold values were subsequently evaluated. Real-time PCR analysis for the FII gene assay performed in DNA extracts from EDTA blood samples revealed a median Ct value of 19.3. Similar Ct values were apparent in the DNA extracts obtained from the heparinized blood and sodium fluoride with potassium oxalatetreated samples: 18.5 and 18.9, respectively. Significantly higher Ct values were found in extracts from clotted blood with medians of 20.6 (tubes with inert separation gel) and 20.5 (tubes without the gel, both P < 0.001). The data on the FV real-time PCR analysis were very comparable to the FII assay. In the modified whole blood, the samples treated with heparin salts showed significantly lower Ct values (P < 0.001) in both assays when compared with the samples with EDTA, sodium fluoride with potassium oxalate, and with the samples with clotted blood. Our results indicate that real-time PCR analyses of thrombophilic mutations were not negatively influenced by the presence of heparin salts in collection tubes. Blood samples with various anticoagulants might be exchangeable for each other when DNA analysis of thrombophilic mutations is required.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 5-6 1","pages":"201-205"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67053769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068010001

J W Liu, F Supandi, S K Dhillon

{"title":"Ferroptosis-Related Long Noncoding RNA Signature Predicts Prognosis of Clear Cell Renal Carcinoma.","authors":"J W Liu, F Supandi, S K Dhillon","doi":"10.14712/fb2022068010001","DOIUrl":"10.14712/fb2022068010001","url":null,"abstract":"Clear cell renal cell carcinoma (ccRCC) is very common and accounts for most kidney cancer deaths. While many studies are being conducted in finding the prognostic signatures of ccRCC, we believe that ferroptosis, which involves programmed cell death dependent on iron accumulation, has therapeutic potential in ccRCC. Recent research has shown that long noncoding RNAs (lncRNAs) are involved in ferroptosis-related tumour processes and are closely related to survival in patients with ccRCC. Hence, in this study we aim to further explore the role of ferroptosis-related lncRNAs (FRLs) in ccRCC, hoping to establish a signature to predict the survival outcome of ccRCC. We analysed transcriptome data from The Cancer Genome Atlas database (TCGA) and ferroptosis-related genes (FRGs) from FerrDb to identify FRLs using Pearson's correlation. Lasso Cox regression analysis and multivariate Cox proportional hazards models screened seventeen optimal FRLs for developing prognostic signatures. Kaplan-Meier survival curves and ROC curves were then plotted for validating the sensitivity, specificity, and accuracy of the identified signatures. Gene Set Enrichment Analysis and CIBERSORT algorithm were deployed to explore the role of these FRLs in the tumour microenvironment. It was concluded that these models demonstrate excellent performance in predicting prognosis among patients with ccRCC, also indicating association with the clinicopathologic parameters such as tumour grade, tumour stage and tumour immune infiltration. In conclusion, our findings provide novel insights into ferroptosis-related lncRNAs in ccRCC, which are important targets for investigating the tumorigenesis of ccRCC.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 1 1","pages":"1-15"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67052553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigen Presentation and Proteome Study of Exosomes Secreted by Co-Culture of Macrophages and Talaromyces marneffei. 巨噬细胞与马尔尼菲talaromyis marneffei共培养外泌体抗原呈递及蛋白质组学研究。

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068040125

Z Xie, L L Li, G J Wei, C Wang, D H Zhang, L Wei

{"title":"Antigen Presentation and Proteome Study of Exosomes Secreted by Co-Culture of Macrophages and Talaromyces marneffei.","authors":"Z Xie, L L Li, G J Wei, C Wang, D H Zhang, L Wei","doi":"10.14712/fb2022068040125","DOIUrl":"10.14712/fb2022068040125","url":null,"abstract":"It is known that intracellular pathogens interact and react with the cellular immune system through exosomes produced by macrophages. This study aimed to determine whether co-culture of macrophages and Talaromyces marneffei induces exosomes and leads to immune responses. T. marneffei was incubated to collect conidia, co-cultured with human macrophages, which then induced exosomes. In cellular experiments, after extraction and purification, the exosomes were then observed by electron microscopy and detected by flow cytometry and mass spectrometry. In animal experiments, flow cytometry and enzyme-linked immunosorbent assay were used to examine whether exosomes were antigenpresenting. The results showed that purified exosomes produced a pro-inflammatory response and stimulated production of TNF-α in non-fungal-treated macrophages. Protein mass spectrometry analysis of exosomes also indicated their potential ability to activate the internal immune response system and the pro-inflammatory response. Translation and ribosomes were the most abundant GO terms in proteins, and the most relevant KEGG pathway was the biosynthesis of secondary metabolites. Furthermore, in vivo experiments revealed that exosomes induced activation of lymphocytes and increased expression of TNF-α and IL-12 in the lung, mediastinum, and spleen area. In conclusion, exosomes can be released by co-culture of T. marneffei and macrophages, having antigen-presenting functions, promoting macrophage inflammation, and initiating adaptive immune responses. These processes are inextricably linked to the translation of secondary metabolites, ribosomes and biosynthesis.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 4 1","pages":"125-132"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67053261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of the Anti-Inflammatory Effect of Tenebrio Molitor Larva Oil in a Colitis Mouse Model. 黄粉虫幼虫油在结肠炎小鼠模型中的抗炎作用验证。

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068020050

B M Park, J Lee, B G Jung, B J Lee

{"title":"Validation of the Anti-Inflammatory Effect of Tenebrio Molitor Larva Oil in a Colitis Mouse Model.","authors":"B M Park, J Lee, B G Jung, B J Lee","doi":"10.14712/fb2022068020050","DOIUrl":"10.14712/fb2022068020050","url":null,"abstract":"Ulcerative colitis is caused by various external factors and is an inflammatory disease that causes decreased intestinal function. Tenebrio molitor larvae contain more than 30 % fat, and the fat component consists of 45 % oleic acid, 20 % linoleic acid and 20 % polyunsaturated fatty acids. In this study, after administering Tenebrio molitor larva oil (TMLO) in a dextran sodium sulphate (DSS)-induced ulcerative colitis mouse model, the pathological findings and inflammatory markers of colitis were analysed to assess whether a colitis mitigation effect was achieved. In the TMLO-administered group, the colon length increased, the spleen weight decreased, and the body weight increased compared with that in the DSS group. In addition, the disease activity index level decreased, the mRNA expression level of inflammatory cytokines in the colon decreased, and the myeloperoxidase activity level significantly decreased. Also, the activity of the NF-κB pathway involved in the regulation of the inflammatory response was lower in the TMLO group than in the DSS group. Taken together, these results suggest that TMLO suppresses occurrence of acute ulcerative colitis in the DSS mouse model. Therefore, TMLO has the potential to be developed as a health food for the prevention and treatment of ulcerative colitis.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 2 1","pages":"50-58"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67052968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPM7 Elicits Proliferation and Differentiation of Human Lens Epithelial Cells through the TGF-β/Smad Pathways. TRPM7通过TGF-β/Smad通路诱导人晶状体上皮细胞增殖和分化

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068020072

G Yang, Y Wu, S Tang

引用次数: 0

C-Phycocyanin Suppresses Cell Proliferation and Promotes Apoptosis by Regulating the AMPK Pathway in NCL-H292 Non-Small Cell Lung Cancer Cells. c -藻蓝蛋白通过调控AMPK通路抑制NCL-H292非小细胞肺癌细胞增殖和促进凋亡

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068010016

H Chaowen, H Dongxuan, H Dongsheng, P Jianfeng, Y Fan, C Yahui, L Xiaohua

{"title":"C-Phycocyanin Suppresses Cell Proliferation and Promotes Apoptosis by Regulating the AMPK Pathway in NCL-H292 Non-Small Cell Lung Cancer Cells.","authors":"H Chaowen, H Dongxuan, H Dongsheng, P Jianfeng, Y Fan, C Yahui, L Xiaohua","doi":"10.14712/fb2022068010016","DOIUrl":"10.14712/fb2022068010016","url":null,"abstract":"Non-small cell lung cancer (NSCLC) results in high mortality and has gained increasing attention. C-Phycocyanin (C-PC) has been identified as a potential therapeutic inhibitor for NSCLC, but its underlying mechanism remains obscure. The gene expression of the long noncoding RNA neighbour of BRCAI RNA 2 (NBR2) in NSCLC cells was evaluated by quantitative reverse transcription-PCR. The cell capacity for proliferation and migration was examined by EdU and wound-healing assays. Furthermore, the viability and apoptosis of cells was measured with CCK-8 and annexin V/PI, respectively. Next, the protein level of activation of adenosine monophosphate- activated protein kinase and the rapamycin kinase (mTOR) signalling pathway-associated molecules was evaluated by western blotting. H292 cells were pre-treated with C-PC or transfected with plasmids encoding NBR2 or the shNBR2 plasmid, to over-express or knock down NBR2 expression, respectively. NBR2 expression was robustly down-regulated in NSCLC cell lines compared with a normal cell line (BEAS-2B). NBR2 over-expression inhibited migration and promoted apoptosis of H292 cells. Treatment of H292 cells with C-PC enhanced NBR2 levels in a dose- and time-dependent manner. Downregulation of NBR2 in H292 cells inhibited the activity of C-PC on cell proliferation, viability and clone formation. Further mechanistic investigation showed that the down-regulation of NBR2 abolished the modulatory effects of C-PC on the AMPK/mTOR signalling pathway. In conclusion, C-PC inhibits H292 cell growth by enhancing the NBR2/AMPK signalling pathway.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 1 1","pages":"16-24"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67052576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0