Genetika-Belgrade最新文献

{"title":"Genotypic differences for chlorophyll, ash and N contents and their relations with grain yield in Turkish bread wheat landraces","authors":"R. Kara, A. Akkaya","doi":"10.2298/gensr2301177k","DOIUrl":"https://doi.org/10.2298/gensr2301177k","url":null,"abstract":"The genetic differences in the landraces are very important for plant breeding. The aim of this study was to determine the genetic differences for chlorophyll, ash and N contents and their relations with grain yield in Turkish bread wheat landraces. There were significant genetic differences for grain yield (GY), ash content of flag leaf at anthesis (AFLAC), ash content of spike at anthesis (ASAC), ash content of flag leaf at maturity (MFLAC), grain ash content (GAC), chlorophyll content at anthesis (ACC), chlorophyll content at early milk maturity (EMCC), chlorophyll content at late milk maturity (LMCC), chlorophyll content at early dough maturity (EDCC), N content of flag leaf at anthesis (FLN) and spike N contents (SN). The grain yield was positively and significantly related with AFLAC, ASAC, MFLAC and ACC, negatively and significantly related with GAC, EDCC and SN, not significantly related with EMCC, LMLC and FLN.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of epistatic interactions between genetic variants located in microRNA and silencing complex genes on prostate cancer progression risk","authors":"Zorana Dobrijević, J. Karanović, D. Savić-Pavićević, G. Brajušković","doi":"10.2298/gensr2301263d","DOIUrl":"https://doi.org/10.2298/gensr2301263d","url":null,"abstract":"Previous studies conducted in Asian and European populations have provided evidence of the association between microRNA-related genetic variants and prostate cancer (PCa) risk and/or progression. Nevertheless, the results obtained in these studies are inconsistent, which could be explained by the limitations of single-locus main effect evaluations to detect joint effects of multiple genetic variants, reflected in statistical epistases. Therefore, we conducted the analysis of potential epistatic interactions between variants located in microRNA genes and in genes encoding the components of RNA-induced silencing complex (RISC) in relation with PCa risk/aggressiveness. Raw data on genotyping results from our previous studies involving four microRNA polymorphisms and five variants in RISC genes were subjected to the exclusion of samples based on missing data criterion, followed by the re-evaluation of Hardy-Weinberg equilibrium. Afterwards, these genotyping results were included in the Multifactor dimensionality reduction (MDR) analysis. Permutation testing was conducted in order to assess statistical significance of the best models from MDR tests. MDR tests on the risk of developing PCa yielded statistically insignificant results. Nevertheless, the MDR results for comparison of PCa patients with high and low cancer progression risk were statistically significant for the analysis that included rs11614913, with the 3-locus best model comprising this genetic variant, rs7813 and rs784567. We conclude that statistical epistasis between rs11614913 in hsa-miR-196a2, rs7813 in GEMIN4 and rs784567 in TARBP2 shows association with the invasiveness of PCa.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of micro RNA expressions with pediatric celiac clinical findings","authors":"G. Dogan, S. Boyacıoğlu, M. Caliskan, E. Kasap, S. Ayhan, E. Kasırga","doi":"10.2298/gensr2301277d","DOIUrl":"https://doi.org/10.2298/gensr2301277d","url":null,"abstract":"There is a need to determine the relationship between the function of the immune system and miRNA expression in pediatric celiac disease (pCD). We aimed to describe the expression profiles of miRNAs in Turkish pCD patients based on the clinical and pathological findings. This study was conducted on 33 pCD patients and 33 pediatric control subjects with normal biopsy results. Four most common mutations (DQA1*05, DQB1*02, DQA1*03, DQB1*03:0.2) on HLA gene in pCD were screened. Paraffin-embedded biopsy tissue samples were used in miRNA isolations followed by cDNA synthesis. Expression of miRNAs were evaluated in the groups with qRT-PCR array-method. Significant underexpression of hsa-miR-194-5p gene was detected in pCD patients compared to the control group. The hsa-miR-194-5p gene was significantly underexpressed in anemic or short stature pCD patients compared to the control. The genes of hsa-miR-29b-3p, hsa-miR-30e-5p, and hsa-miR-146a-5p were significantly overexpressed in the patients with constipated celiac patients. Significant overexpression of hsa-miR146a-5p gene was detected in the Marsh2 and Marsh3a groups. The hsa-miR-29b-3p, hsa-miR-30e-5p, hsa-let-7a-5p, hsa-miR-27a-3p, hsa-miR141-3p, hsa-miR143-3p, and hsa-miR-146a-5p miRNA genes were significantly overexpressed in the Marsh3b group. Also, the hsa-miR-194-5p and hsa-miR-26a-5p genes were significantly underexpressed in the comparison of Marsh3c group to the control. These results suggest that miRNA expressions are likely to play a role in the pathogenesis of pCD. It is believed that the current results present valuable inferences that may help understand the genetic boundaries on pCD, which might be further supported by follow up studies on other miRNAs.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic distribution of MSTN gene polymorphisms involved in racing performance in Camelus dromedarius","authors":"Ghashia Asif, A. Nadeem, H. Mujahid, S. Imran, N. Mukhtar, A. Sheikh, M. Javed","doi":"10.2298/gensr2301427a","DOIUrl":"https://doi.org/10.2298/gensr2301427a","url":null,"abstract":"Camel racing is one of the popular sports around the world and is growing rapidly especially in Gulf countries. Camel has adapted itself to harsh and draught climate of desert. ?his quality of camel makes it the best choice in racing industry. Pakistani breed Dromedarius camels are as good as Omani and Sudanese camel breeds in their racing potential. Myostatin (Growth differentiation factor 8) is a protein coded by MSTN gene. Polymorphism in MSTN play a significant role in growth of muscle, increasing fast glycolysis Type IIB muscle fiber, forming tubular aggregates in type IIB fiber by increasing the body strength and racing ability. This gene is also responsible for double muscle phenotype in bovines. In present study, Marecha and Brela camel breeds of Pakistan were studied for genomic characterization of MSTN gene. Blood samples were collected from the healthy animals between the age group of 2-4 years. Genomic DNA was extracted, amplified by using specific sets of primers, purified and sequenced by Sanger?s dideoxy chain termination method. No single nucleotide polymorphisms (SNPs) were found in the exon-3 of MSTN of Marecha & Brela, which shows that the gene is highly conserved among species while phylogenetic data of the dromedarius MSTN gene showed highest similarity with Bos taurus and least similarity with Gallus gallus. Genes that are similar to the camel MSTN are myostatin of Homo sapiens, Pan troglodytes, Bos taurus, Canis lupus, Rattus norvegicus. The significance of this study was to identify the genetic potential of Pakistani camel for racing that will help in the socioeconomic uplift of the local community. It will also help the camel breeders to select the best breed of camel & enhance their genetic potential by using least operating cost. That will in turn provide opportunity to the camel breeders to produce the best breed that will be selected by the trainers for racing that will provide another source of income for the local community and Pakistan?s camels will also be used in the racing industry.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and examination of certain cytogenetic characteristics of some autochthonous varieties of grapevine in Republic of North Macedonia","authors":"B. Korunoska, Vladan Pesic, Nenad Bunjac","doi":"10.2298/gensr2301439k","DOIUrl":"https://doi.org/10.2298/gensr2301439k","url":null,"abstract":"In R.N. Macedonia has a large number of autochthonous or domestic varieties (cultivars) of grapevine that are grown as individual vines or in small, limited plantations. Until the beginning of the 20th century and before the phylloxera attack in our country, native varieties were much more represented. Autochthonous varieties existed and were cultivated for a long time in several vineyards and were characterized by stable and significant properties. These varieties are adapted to the environmental conditions where they are grown and are carriers of stable genes that can be used in further selection and hybridization to obtain new grape varieties. Therefore, it is very important for a country (region) to have autochthonous varieties in its assortment.In this paper, we considered four autochthonous grape varieties - two table varieties (Konchanka and white winter) and two wine varieties (Ohrid white, Ohrid black). We tried, according to the ampelographic description and certain characteristics of the reproductive system, to determine their origin. According to the phenotypic characteristics that refer to the centers of origin of the varieties, the studied varieties - Konchanka and White winter belong to the Eastern varieties convarietas orientalis, subconvarietas antasiatica, and the studied varieties Ohrid white and Ohrid black variety belong to the Black Sea - Balkan varieties convarietas. pontica, subconvarietas balcanica. Among the examined varieties, the properties from the second group of descriptors are described in detail according to the proposals of O.I.V. According to some codes (characteristics) there is a big difference between varieties. Also, for a more detailed differentiation of the varieties, cytological properties were examined - pollen germination and vitality, number of chromosomes in mitosis and meiosis, ploidy level, percentage of fertilization (fecundation), self-fertilization (autogamy) and cross-fertilization (xenogamy). The statistical processing of the ampelography data is according to a cluster program (SPSS) that works on the binary similarity or dissimilarity as measures used to group the data and further form a clear dendogram display.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of leptin gene polymorphisms in the evaluation of the growth performance and carcass measurements of v-line and Baladi black rabbits","authors":"H. Radwan, A. Ateya, El-HithamAmr Abo, Shimaa A. Sakr, M. Fouda, R. Darwish, A. El-Desoky","doi":"10.2298/gensr2301319r","DOIUrl":"https://doi.org/10.2298/gensr2301319r","url":null,"abstract":"This study investigated the association between both polymorphisms and metabolic marker changes of the leptin gene, and body weight, weight gain, carcass traits, feed intake, and feed conversion ratio. Blood samples were collected from 60 V-line and 60 Baladi Black rabbits for DNA extraction and biochemical analysis. DNA sequencing of leptin (202-bp) revealed four non-synonymous single nucleotide polymorphism (SNPs) that characterized a number of V-line rabbits. Statistical analysis revealed that the identified SNPs were associated with rabbit growth and carcass measurements (p < 0.05). A discriminant analysis model showed a high classification percentage for the identified SNPs within and between breeds using body weight at 5-14 weeks of age (91.7%), slaughter traits (91.6%), feed intake, daily feed intake, and feed conversion ratio (76.7%). This study reveals that leptin gene could be a candidate for growth traits in rabbits enabling the development of marker-assisted selection (MAS) in different rabbit breeds.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress, adaptability and stability of soybean grain yield and grain quality in conventionally created elite lines","authors":"Maja Matosa-Kocar, A. Sudarić, T. Duvnjak, Zoe Andrijanić, Antonela Markulj-Kulundzic","doi":"10.2298/gensr2301245m","DOIUrl":"https://doi.org/10.2298/gensr2301245m","url":null,"abstract":"The objective of this study was to determine the progress in grain yield and grain quality accomplished with conventional breeding methods, as well as to identify stable, widely or specifically adapted genotypes under central European growing conditions. Recently developed soybean elite lines of maturity groups (MGs) 00, 0 and I were compared with commercial cultivars (standards) in comparative field tests during three consecutive years (2018-2020) in Osijek, Croatia. The ANOVA results showed significant genotype, environment, and genotype-by-environment interaction effects. There was a significant improvement in productivity and quality in comparison to standards, while stability parameters for tested traits indicated there are stable and mostly specifically adaptable elite lines. Improvement of the domestic gene pool and high agronomic performances of elite lines stable in most important economic traits will considerably contribute to increasing and improving soybean production in central Europe.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical substances and bioactive components of wild cornelian cherry (Cornus mas L.) fruits in Erzincan province of Eastern Turkey","authors":"K. Ozrenk, A. Tas, M. Gundogdu, N. Keskin, S. Ercişli","doi":"10.2298/gensr2301095k","DOIUrl":"https://doi.org/10.2298/gensr2301095k","url":null,"abstract":"Turkey has a very rich flora due to diverse climatic and topographic conditions within the country. Cornelian cherry (Cornus mas L.) is an important wild edible fruits and widely distributed in Turkey and well known for its fruit antioxidants and nutrients. In this study, phytochemical content in fruits of a number of Cornelian cherry genotypes in Erzincan region were determined. The biochemical analysis included organic acids, sugars, vitamin C, antioxidant and individual phenolic compounds. In the study, organic acid content was between 253.09 mg/100 g and 112.50 mg/100 g. Vitamin C content varied between 115.85 mg/100g and 43.77 mg/100 g. Likewise, the sugar content of Cornelian cherry fruits varied from 6.17 g/100 g to 4.06 g/100 g. When the antioxidant content was examined, the highest antioxidant was 980.91 ?mol TE/g fresh weight (FW) base and the lowest was 490.38 ?mol TE/g FW. In the genotypes examined, gallic acid was determined as the highest among the individual phenolic compounds, while the individual phenolic amounts ranged from 38.93 mg/100 g FW to 4.31 mg/100 g FW. As a result of the study, it was determined that Cornelian cherry fruits are very rich in vitamin C and other phytochemicals and as a result of this examination, 24ER04 and 24ER08 genotypes came to the fore.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic diversity in 55 herbaceous peony cultivars with SSR markers","authors":"Yongming Fan, Liuhui Yang, Qi Wang, D. Teixeira, Xiao‐Nan Yu","doi":"10.2298/gensr2301387f","DOIUrl":"https://doi.org/10.2298/gensr2301387f","url":null,"abstract":"Using SSR, a molecular marker, this study aimed to evaluate the genetic diversity and genetic relationship among 55 herbaceous peony (Paeonia lactiflora Pall.) cultivars belonging to the Lactiflora, Hybrid and Itoh Groups to lay a foundation for the molecular classification of peony cultivars, as well as for genetic background analysis and breeding of new cultivars. Our results show that 86 alleles, 76 of which were polymorphic, were obtained by screening 13 pairs of polymorphic primers from 100 initially tested primers, each of them having an average of 6.615 alleles. The range of variation of the effective alleles was 2.243-7.800, PIC content was 0.554-0.872 and Shannon?s genetic diversity index was 1.701-3.126. The 55 herbaceous peony cultivars were divided into nine groups based on UPGMA. This analysis indicates that the 55 cultivars have rich genetic diversity while a phylogenetic relationship of most cultivars was identified. This molecular-based classification is a useful and simple reference molecular method to differentiate peony cultivars in crossbreeding programs.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical variability and population structure of Cucumis melo L. accessions collected from Iran","authors":"Pedram Ajalli, N. Mohebalipour, H. Nourafcan, M. Hatami, A. Faramarzi","doi":"10.2298/gensr2301401a","DOIUrl":"https://doi.org/10.2298/gensr2301401a","url":null,"abstract":"Melon (Cucumis melo) is one of the most important cultivated cucurbits. In this project, the genetic variability among 14 accessions of melon collected by the authors has been studied using 23 anatomical characteristics across 2 consecutive years as well as 146 random amplified polymorphic DNA (RAPD) markers. Combined analysis of variance revealed significant effects on genotype and year ? genotype effects. Among examined anatomical characters, the highest CV% values across two years belonged to fruit store at room temperature (88.96%, 103.6%) and total fruit weight (42.2%, 40.00%), while the lowest CV% values were observed in flower petal width (0.31%, 0.21%), flower petal length (0.28%, 0.49%), and peduncle length (0.40%, 0.19%). Classification of melon accessions based on anatomical characteristics using the Ward method produced three groups. The highest Jaccard?s similarity coefficient (0.76) was observed between accessions ?Atashi koluche? and ?Atashi miyaneh? and the lowest value (0.49) was found between accessions ?Bakermellon? and ?Mashhadi? with the mean value of 0.59. In this study, OPA06 and OPB13 primers possessed greater efficiency in the genetic evaluation of the studied germplasm. Analysis of population structure, which imply on the existence of admixture in the studied melon germplasm, revealing three subpopulations. Accession ?Sabzevari? identified as mixed subgroups. From the breeder?s view, the introduced heterotic groups can be utilized in parental selection for the construction of mapping population and the identified population structure can prevent any false positive output in marker-trait association studies of melon.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}