Assessment of genetic diversity in 55 herbaceous peony cultivars with SSR markers

4区农林科学 Q3 Agricultural and Biological Sciences

Genetika-Belgrade Pub Date : 2023-01-01 DOI:10.2298/gensr2301387f

Yongming Fan, Liuhui Yang, Qi Wang, D. Teixeira, Xiao‐Nan Yu

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引用次数: 0

Abstract

Using SSR, a molecular marker, this study aimed to evaluate the genetic diversity and genetic relationship among 55 herbaceous peony (Paeonia lactiflora Pall.) cultivars belonging to the Lactiflora, Hybrid and Itoh Groups to lay a foundation for the molecular classification of peony cultivars, as well as for genetic background analysis and breeding of new cultivars. Our results show that 86 alleles, 76 of which were polymorphic, were obtained by screening 13 pairs of polymorphic primers from 100 initially tested primers, each of them having an average of 6.615 alleles. The range of variation of the effective alleles was 2.243-7.800, PIC content was 0.554-0.872 and Shannon?s genetic diversity index was 1.701-3.126. The 55 herbaceous peony cultivars were divided into nine groups based on UPGMA. This analysis indicates that the 55 cultivars have rich genetic diversity while a phylogenetic relationship of most cultivars was identified. This molecular-based classification is a useful and simple reference molecular method to differentiate peony cultivars in crossbreeding programs.

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55个芍药品种遗传多样性的SSR标记评价

利用SSR分子标记技术，对55个芍药(Paeonia lactiflora Pall.)属芍药(lactiflora)、杂种(Hybrid)和伊藤(Itoh)类群的芍药品种的遗传多样性和亲缘关系进行分析，为芍药品种的分子分类、遗传背景分析和新品种选育奠定基础。结果表明，从100对初始引物中筛选出13对多态性引物，共获得86个等位基因，其中76个等位基因为多态性，平均每对引物含有6.615个等位基因。有效等位基因变异范围为2.243 ~ 7.800,PIC含量为0.554 ~ 0.872,Shannon?遗传多样性指数为1.701 ~ 3.126。根据UPGMA将55个芍药品种划分为9个类群。结果表明，55个品种具有丰富的遗传多样性，多数品种具有亲缘关系。这种基于分子的分类方法为牡丹品种的杂交鉴定提供了一种简便实用的参考分子方法。

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来源期刊

Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.