Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology最新文献_第2页

Comparison of molecular protocols to detect Tomato brown rugose fruit virus in solanaceae hosts 比较检测茄科寄主番茄褐皱纹果病毒的分子方案

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Pub Date : 2023-12-26 DOI: 10.18781/r.mex.fit.2023-5

E. J. Zamora-Macorra, Katia Aviña-Padilla, Rosemarie W. Hammond, D. Ochoa-Martínez

引用次数: 0

Genus Orthotospovirus in Costa Rica: A Central American case 哥斯达黎加的 Orthotospovirus 属：中美洲的一个案例

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Pub Date : 2023-12-26 DOI: 10.18781/r.mex.fit.2023-6

M. Montero-Astúa, Natasha Dejuk-Protti, David Bermúdez-Gómez, Elena Vásquez Céspedes, I. Sandoval-Carvajal, L. Garita-Salazar, Federico J. Albertazzi, Scott Adkins, Lisela Moreira-Carmona

{"title":"Genus Orthotospovirus in Costa Rica: A Central American case","authors":"M. Montero-Astúa, Natasha Dejuk-Protti, David Bermúdez-Gómez, Elena Vásquez Céspedes, I. Sandoval-Carvajal, L. Garita-Salazar, Federico J. Albertazzi, Scott Adkins, Lisela Moreira-Carmona","doi":"10.18781/r.mex.fit.2023-6","DOIUrl":"https://doi.org/10.18781/r.mex.fit.2023-6","url":null,"abstract":"The Orthotospovirus genus encompasses a range of economically significant and emerging plant viruses that affect a variety of crops globally. While the prevalence and characteristics of these phytopathogenic viruses are extensively documented in North and South America, their presence in Central America remains comparatively underexplored. This study focuses on Costa Rica, strategically positioned at the nexus of North and South America, to enhance our understanding of orthotospovirus in this region. We analyzed 295 plant samples using enzyme-linked immunosorbent assay (ELISA) to test for the presence of INSV, IYSV, TSWV, and the GRSV/TCSV serogroup. Additionally, a subset (20 samples) underwent further scrutiny through reverse transcription-polymerase chain reaction (RT-PCR) employing both universal and species-specific primers. Our ELISA results indicated the absence of TSWV and the GRSV/TCSV serogroup. However, the presence of INSV in Costa Rica was substantiated through ELISA, RT-PCR, and partial sequencing, revealing its prevalence in both open-field and greenhouse environments. Despite previous diagnostic reports suggesting the presence of TSWV in Costa Rica, our study did not detect this virus. RT-PCR analysis with degenerate primers also found no evidence of other orthotospovirus species in our samples. The identification of a dominant INSV haplotype, along with three additional variants, suggests the likelihood of at least two independent virus introductions into the region. These findings underscore the necessity for more comprehensive surveys and research on orthotospoviruses in Central America to better understand their epidemiology and impact on agriculture.","PeriodicalId":504087,"journal":{"name":"Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of endophytic microorganisms for the management of Tomato brown rugose fruit virus in tomato crop (Solamun lycopersicum) 利用内生微生物控制番茄作物（Solamun lycopersicum）中的番茄褐皱纹病毒

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Pub Date : 2023-12-26 DOI: 10.18781/r.mex.fit.2023-1

Carlos D. Ramos-Villanueva, Guadalupe Carrillo-Benitez, E. J. Zamora-Macorra, Eduardo Santiago-Elena, Samuel Ramírez-Alarcón, Jezrael Jimenez-Vidals, Ricardo Ávila-López

{"title":"Use of endophytic microorganisms for the management of Tomato brown rugose fruit virus in tomato crop (Solamun lycopersicum)","authors":"Carlos D. Ramos-Villanueva, Guadalupe Carrillo-Benitez, E. J. Zamora-Macorra, Eduardo Santiago-Elena, Samuel Ramírez-Alarcón, Jezrael Jimenez-Vidals, Ricardo Ávila-López","doi":"10.18781/r.mex.fit.2023-1","DOIUrl":"https://doi.org/10.18781/r.mex.fit.2023-1","url":null,"abstract":"The Tomato brown rugose fruit virus (ToBRFV) is one of the main pathogens affecting tomato crops in Mexico. Despite efforts to prevent its spread, it is nearly impossible due to its low transmission percentage through seeds and its high susceptibility to being transmitted through cultural practices. Therefore, alternative management strategies are being sought. This research aimed to determine the effect of endophytic microorganisms applied to the soil on tomato plants infected with ToBRFV. A tomato plant was used as an experimental unit, with 13 repetitions per treatment. The treatments on tomato plants infected with ToBRFV were Beauveria peruviencis, Trichoderma longibrachiatum, Pseudomonas sp. And water as a sick witness; a treatment of healthy plants treated with water was also included as an absolute control. The response variables were plant height, fresh weight of the aerial part and root and severity (two evaluations). Measurements were analyzed using Tukey-Kramer HSD tests for each pair. Significant differences were found Beauveria peruviencis, Trichoderma longibrachiatum, Pseudomonas sp. and water as a sick witness. The treatment that most favored the development of infected plants (79% taller and 15% heavier than infected mock) and reduced its severity was B. peruviensis, followed by Pseudomonas sp. On the other hand, the treatment that resulted in the least plant development (31% smaller than infected mock) and even increased the severity of the infection was T. longibrachiatum.","PeriodicalId":504087,"journal":{"name":"Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weeds and ruderal plants as potential sources of inoculum for vegetable diseases in northern Sinaloa 杂草和灌木植物是锡那罗亚州北部蔬菜病害的潜在接种源

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Pub Date : 2023-12-26 DOI: 10.18781/r.mex.fit.2023-4

R. Félix-Gastélum, G. Herrera-Rodríguez, K. Y. Leyva-Madrigal, G. A. Mora-Romero

{"title":"Weeds and ruderal plants as potential sources of inoculum for vegetable diseases in northern Sinaloa","authors":"R. Félix-Gastélum, G. Herrera-Rodríguez, K. Y. Leyva-Madrigal, G. A. Mora-Romero","doi":"10.18781/r.mex.fit.2023-4","DOIUrl":"https://doi.org/10.18781/r.mex.fit.2023-4","url":null,"abstract":"Weeds and ruderal plants of the families Cucurbitaceae and Solanaceae are addressed as potential sources of inoculum for the development of viral diseases such as Tomato apex necrosis virus (ToANV), zucchini (Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Papaya ring spot virus (PRSV-W) and Cucumber mosaic virus (CMV). Reference is made to weeds and ruderal plants as potential sources of inoculum, including wild sunflower for powdery mildew (Golovinomyces spadiceus), wild tobacco for foliar blight (Alternaria spp.), black nightshade for leaf spot (Curvularia moehlemvekiae), Johnson grass for foliar blight (Alternaria sp.), and wild castor bean for foliar blight (Alternaria ricini) and wild melon for downy mildew (Pseudoperonospora cubensis). Future lines of multidisciplinary research focusing on the determination of pathogenicity in cultivated plants of viruses and fungi associated with wild plants and vice versa are proposed; the spatial-temporal distribution of wild plants that may serve as sources of inoculum, as well as the of potential insect vectors of viral diseases, should also be studied. The implementation of modern molecular techniques, such as High Throughput Sequencing, for the detection of phytopathogens is important. All this will contribute to the implementation of environmentally friendly strategies for disease control in agricultural crops in Sinaloa, for the benefit of the vegetable growers.","PeriodicalId":504087,"journal":{"name":"Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Etiology of rhizome rot of asparagus (Asparagus officinalis) in Atenco, Mexico State 墨西哥州阿坦科芦笋（Asparagus officinalis）根茎腐烂病的病原学研究

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Pub Date : 2023-11-16 DOI: 10.18781/r.mex.fit.2211-1

Juan Agustin Gonzalez-Cruces, J. S. Sandoval-Islas, Cristian Nava-Díaz, Maricarmen Sandoval- Sánchez

{"title":"Etiology of rhizome rot of asparagus (Asparagus officinalis) in Atenco, Mexico State","authors":"Juan Agustin Gonzalez-Cruces, J. S. Sandoval-Islas, Cristian Nava-Díaz, Maricarmen Sandoval- Sánchez","doi":"10.18781/r.mex.fit.2211-1","DOIUrl":"https://doi.org/10.18781/r.mex.fit.2211-1","url":null,"abstract":"The objective of this research was to identify the causal agent of asparagus rhizome rot, as well as evaluate different inoculation methods and the severity of the isolates. Sampling was carried out in five producing plots Atenco, Edo. from Mexico. Five isolates of Fusarium spp. were selected. (one per plot) to perform pathogenicity tests. Three isolates were selected for their colonization characteristics for severity tests with different inoculation methods: Immersion for 12 h, immersion for 30 min and inoculation by contact with absorbent paper soaked in 1 mL of inoculum. Concentrations of 1x106 conidia mL-1 were used. 10 rhizomes were used per treatment and 10 rhizomes without inoculation. To determine the severity, photographs (in GIMP®) of the rhizome were analyzed seven days after inoculation. The isolates were molecularly identified with ITS4/ITS5, EF688/EF1521 and TUBT1/BT2B. Fusarium prolifetatum was morphologically and molecularly identified in the three isolates. The P3DR isolate was the most severe (14.6%), followed by P5DR (13.9%) and P1SIR (11.6%). The most effective inoculation method was immersion for 30 min. They were registered in the NCBI Gene Bank with accessions ON738484 (P3DR), ON973801 (P5DR) and ON738483 (P1SIR). This is the first report of F. prolifetatum in the Edo. from Mexico.","PeriodicalId":504087,"journal":{"name":"Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139270474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspergillus oryzae: An opportunity for agriculture 米曲霉：农业的机遇

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Pub Date : 2023-11-15 DOI: 10.18781/r.mex.fit.2302-2

Karen Berenice García-Conde, E. Cerna-Chávez, Yisa María Ochoa-Fuentes, Jazmín Janet Velázquez-Guerrero

引用次数: 0