Acta Biochimica et Biophysica Sinica最新文献_第3页

CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway. CTNNAL1 通过 RhoA/ROCK1 通路促进支气管上皮细胞结构的完整性。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-11 DOI: 10.3724/abbs.2024026

Caixia Liu, Jinmei Wang, Yurong Tan, Chi Liu, X. Qu, Hui-jun Liu, Meiling Tan, Changqing Deng, Xiaoqun Qin, Yang Xiang

{"title":"CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway.","authors":"Caixia Liu, Jinmei Wang, Yurong Tan, Chi Liu, X. Qu, Hui-jun Liu, Meiling Tan, Changqing Deng, Xiaoqun Qin, Yang Xiang","doi":"10.3724/abbs.2024026","DOIUrl":"https://doi.org/10.3724/abbs.2024026","url":null,"abstract":"Adhesion molecules play critical roles in maintaining the structural integrity of the airway epithelium in airways under stress. Previously, we reported that catenin alpha-like 1 (CTNNAL1) is downregulated in an asthma animal model and upregulated at the edge of human bronchial epithelial cells (HBECs) after ozone stress. In this work, we explore the potential role of CTNNAL1 in the structural adhesion of HBECs and its possible mechanism. We construct a CTNNAL1 ‒/‒ mouse model with CTNNAL1-RNAi recombinant adeno-associated virus (AAV) in the lung and a CTNNAL1-silencing cell line stably transfected with CTNNAL1-siRNA recombinant plasmids. Hematoxylin and eosin (HE) staining reveals that CTNNAL1 ‒/‒ mice have denuded epithelial cells and structural damage to the airway. Silencing of CTNNAL1 in HBECs inhibits cell proliferation and weakens extracellular matrix adhesion and intercellular adhesion, possibly through the action of the cytoskeleton. We also find that the expressions of the structural adhesion-related molecules E-cadherin, integrin β1, and integrin β4 are significantly decreased in ozone-treated cells than in vector control cells. In addition, our results show that the expression levels of RhoA/ROCK1 are decreased after CTNNAL1 silencing. Treatment with Y27632, a ROCK inhibitor, abolished the expressions of adhesion molecules induced by ozone in CTNNAL1-overexpressing HBECs. Overall, the findings of the present study suggest that CTNNAL1 plays a critical role in maintaining the structural integrity of the airway epithelium under ozone challenge, and is associated with epithelial cytoskeleton dynamics and the expressions of adhesion-related molecules via the RhoA/ROCK1 pathway.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"25 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140715667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GroEL triggers NLRP3 inflammasome activation through the TLR/NF-κB p-p65 axis in human periodontal ligament stem cells. 在人类牙周韧带干细胞中，GroEL通过TLR/NF-κB p-p65轴触发NLRP3炎症小体活化。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-10 DOI: 10.3724/abbs.2024050

Li Zhang, Mengmeng Duan, Xiaohua Pu, Huiling Zheng, Xinjie Ning, Ying Tu, Chunming Xu, Demao Zhang, Chengcheng Liu, Jing Xie

{"title":"GroEL triggers NLRP3 inflammasome activation through the TLR/NF-κB p-p65 axis in human periodontal ligament stem cells.","authors":"Li Zhang, Mengmeng Duan, Xiaohua Pu, Huiling Zheng, Xinjie Ning, Ying Tu, Chunming Xu, Demao Zhang, Chengcheng Liu, Jing Xie","doi":"10.3724/abbs.2024050","DOIUrl":"https://doi.org/10.3724/abbs.2024050","url":null,"abstract":"The interaction between bacteria and the host plays a vital role in the initiation and progression of systemic diseases, including gastrointestinal and oral diseases, due to the secretion of various virulence factors from these pathogens. GroEL, a potent virulence factor secreted by multiple oral pathogenic bacteria, is implicated in the damage of gingival epithelium, periodontal ligament, alveolar bone and other peripheral tissues. However, the underlying biomechanism is still largely unknown. In the present study, we verify that GroEL can trigger the activation of NLRP3 inflammasome and its downstream effector molecules, IL-1β and IL-18, in human periodontal ligament stem cells (hPDLSCs) and resultantly induce high activation of gelatinases (MMP-2 and MMP-9) to promote the degradation of extracellular matrix (ECM). GroEL-mediated activation of the NLRP3 inflammasome requires the participation of Toll-like receptors (TLR2 and TLR4). High upregulation of TLR2 and TLR4 induces the enhancement of NF-κB (p-p65) signaling and promotes its nuclear accumulation, thus activating the NLRP3 inflammasome. These results are verified in a rat model with direct injection of GroEL. Collectively, this study provides insight into the role of virulence factors in bacteria-induced host immune response and may also provide a new clue for the prevention of periodontitis.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-194-3p regulates epithelial-mesenchymal transition in embryonic epicardial cells via p120/β-catenin signaling miR-194-3p 通过 p120/β-catenin 信号调节胚胎心外膜细胞的上皮-间充质转化

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-01 DOI: 10.3724/abbs.2024051

Tianhua Xiong, Dinghui Wang, Huiping Yang, Bin Liu, Yingrui Li, Wenlong Yu, Jing Wang, Qiang She*

引用次数: 0

Prostate cancer-associated transcript 6 (PCAT6) promotes epithelial–mesenchymal transition and stemness and worsens prognosis in patients with colorectal cancer 前列腺癌相关转录本 6 (PCAT6) 促进上皮-间充质转化和干细胞形成，恶化结直肠癌患者的预后

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024031

Xun Sun, Yitao Yuan, Suyao Li, Lu Gan, Midie Xu, Qingguo Li, Mengling Liu, Keshu Hu, Ke Nan, Jiayu Zhang, Yu Dong, Yufu Lin, Xiuping Zhang, Pengcong Hou, Tianshu Liu

引用次数: 0

Bronchial thermoplasty decreases airway remodeling by inhibiting autophagy via the AMPK/mTOR signaling pathway 支气管热成形术通过 AMPK/mTOR 信号通路抑制自噬，从而减少气道重塑

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024028

Tao Wang, Peng Fu, Wenting Huang, Liang Long, F. Long, Shengming Liu

引用次数: 0

Construction and efficacy testing of DNA vaccines containing HLA-A*02:01-restricted SARS-CoV-2 T-cell epitopes predicted by immunoinformatics 构建含有免疫信息学预测的 HLA-A*02:01 限制性 SARS-CoV-2 T 细胞表位的 DNA 疫苗并进行药效测试

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024039

Dan Tan, Ning Kang, Yuanfei Zhu, Jia Hou, Hanqing Wang, Huijun Xu, Cheng Zu, Zixiang Gao, Mu Liu, Nannan Liu, Qiang Deng, Hongzhou Lu, Jing Liu, Youhua Xie

引用次数: 0

20(S)-Ginsenoside Rh2 inhibits hepatocellular carcinoma by suppressing angiogenesis and the GPC3‐mediated Wnt/β‑catenin signaling pathway 20(S)-人参皂苷 Rh2 通过抑制血管生成和 GPC3 介导的 Wnt/β-catenin 信号通路抑制肝细胞癌的发生

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024038

Linfei Xu, Jing Li, N. Hou, Fang Han, Xiaodong Sun, Qinying Li

引用次数: 0

Proanthocyanidins isolated from lotus seed skin mitigate glycolipid metabolism disorder through p-38/Nrf2/NF-κB signaling pathway 通过p-38/Nrf2/NF-κB信号通路从莲子皮中分离出的原花青素可缓解糖脂代谢障碍

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024042

Yuhang Yi, Tao Li, Chenghao Lv, W. He, Wenzhi Li, Xixin Zhou, Si Qin

引用次数: 0

Targeting the TRAF3-ULK1-NLRP3 regulatory axis to control alveolar macrophage pyroptosis in acute lung injury 靶向 TRAF3-ULK1-NLRP3 调控轴，控制急性肺损伤中肺泡巨噬细胞的脓毒症

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024035

Lei Jiang, Chunlin Ye, Yunhe Huang, Zhi Hu, Guangxia Wei*

引用次数: 0

Elucidating the network interactions between 21 secreted Mycobacterium tuberculosis proteins and host proteins: the role of DnaK in enhancing Mtb survival via LDHB 阐明 21 种结核分枝杆菌分泌蛋白与宿主蛋白之间的网络相互作用：DnaK 在通过 LDHB 提高结核分枝杆菌存活率方面的作用

Acta Biochimica et Biophysica Sinica Pub Date : 2024-03-01 DOI: 10.3724/abbs.2024041

Hong Chen, Xiang He, He-wei Jiang, Yun-xiao Zheng, Hai-nan Zhang, Fan-lin Wu, Zhaowei Xu, Shu-juan Guo, Sheng-Ce Tao

引用次数: 0