Acta Biochimica et Biophysica Sinica最新文献_第2页

Identification of the interaction between MAPK1 and Eimeria acervulinaserine protease inhibitor: a preliminary functional study 识别 MAPK1 与埃默氏菌 acervulinaserine 蛋白酶抑制剂之间的相互作用：初步功能研究

Acta Biochimica et Biophysica Sinica Pub Date : 2024-06-01 DOI: 10.3724/abbs.2024095

Liyin Lian, He Sun, Wanjing Li, Jing Wang, Yifan Sheng, Xinyue Gong, Qian Sun, Pu Wang, Yadong Zheng, Houhui Song

引用次数: 0

Posttranslational regulatory mechanism of PD-L1 in cancers and associated opportunities for novel small-molecule therapeutics 癌症中 PD-L1 的翻译后调控机制以及新型小分子疗法的相关机遇

Acta Biochimica et Biophysica Sinica Pub Date : 2024-05-01 DOI: 10.3724/abbs.2024085

Minchen Cai, Mengting Xu, Dianping Yu, Qun Wang, Sanhong Liu

引用次数: 0

Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating HACE1 gene expression. 厚朴酚通过上调 HACE1 基因的表达促进食管癌细胞的自噬。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-25 DOI: 10.3724/abbs.2024044

Kenan Huang, Biao Zhang, Yu Feng, Haitao Ma

引用次数: 0

Molecular stratification of the human fetal vaginal epithelium by spatial transcriptome analysis. 通过空间转录组分析对人类胎儿阴道上皮细胞进行分子分层。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-25 DOI: 10.3724/abbs.2024063

Ziying Ye, Peipei Jiang, Qi Zhu, Zhongrui Pei, Yali Hu, Guangfeng Zhao

{"title":"Molecular stratification of the human fetal vaginal epithelium by spatial transcriptome analysis.","authors":"Ziying Ye, Peipei Jiang, Qi Zhu, Zhongrui Pei, Yali Hu, Guangfeng Zhao","doi":"10.3724/abbs.2024063","DOIUrl":"https://doi.org/10.3724/abbs.2024063","url":null,"abstract":"The human vaginal epithelium is a crucial component of numerous reproductive processes and serves as a vital protective barrier against pathogenic invasion. Despite its significance, a comprehensive exploration of its molecular profiles, including molecular expression and distribution across its multiple layers, has not been performed. In this study, we perform a spatial transcriptomic analysis within the vaginal wall of human fetuses to fill this knowledge gap. We successfully categorize the vaginal epithelium into four distinct zones based on transcriptomic profiles and anatomical features. This approach reveals unique transcriptomic signatures within these regions, allowing us to identify differentially expressed genes and uncover novel markers for distinct regions of the vaginal epithelium. Additionally, our findings highlight the varied expressions of keratin ( KRT) genes across different zones of the vaginal epithelium, with a gradual shift in expression patterns observed from the basal layer to the surface/superficial layer. This suggests a potential differentiation trajectory of the human vaginal epithelium, shedding light on the dynamic nature of this tissue. Furthermore, abundant biological processes are found to be enriched in the basal zone by KEGG pathway analysis, indicating an active state of the basal zone cells. Subsequently, the expressions of latent stem cell markers in the basal zone are identified. In summary, our research provides a crucial understanding of human vaginal epithelial cells and the complex mechanisms of the vaginal mucosa, with potential applications in vaginal reconstruction and drug delivery, making this atlas a valuable tool for future research in women's health and reproductive medicine.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140658462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AlphaFold2 assists in providing novel mechanistic insights into the interactions among the LUBAC subunits. AlphaFold2 有助于从新的机制角度深入了解 LUBAC 亚基之间的相互作用。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-24 DOI: 10.3724/abbs.2024047

Chenchen Wang, Chunying Gu, Ying Lv, Hongyu Liu, Yanan Wang, Yongmei Zuo, Guangyu Jiang, Lili Liu, Jiafu Liu

{"title":"AlphaFold2 assists in providing novel mechanistic insights into the interactions among the LUBAC subunits.","authors":"Chenchen Wang, Chunying Gu, Ying Lv, Hongyu Liu, Yanan Wang, Yongmei Zuo, Guangyu Jiang, Lili Liu, Jiafu Liu","doi":"10.3724/abbs.2024047","DOIUrl":"https://doi.org/10.3724/abbs.2024047","url":null,"abstract":"The linear ubiquitin chain assembly complex (LUBAC) is the only known E3 ligase complex in which the ubiquitin-like (UBL) domains of SHARPIN and HOIL-1L interact with HOIP to determine the structural stability of LUBAC. The interactions between subunits within LUBAC have been a topic of extensive research. However, the impact of the LTM motif on the interaction between the UBL domains of SHARPIN and HOIL-1L with HOIP remains unclear. Here, we discover that the absence of the LTM motif in the AlphaFold2-predicted LUBAC structure alters the HOIP-UBA structure. We employ GeoPPI to calculate the changes in binding free energy (ΔG) caused by single-point mutations between subunits, simulating their protein-protein interactions. The results reveal that the presence of the LTM motif decreases the interaction between the UBL domains of SHARPIN and HOIL-1L with HOIP, leading to a decrease in the structural stability of LUBAC. Furthermore, using the AlphaFold2-predicted results, we find that HOIP (629‒695) and HOIP-UBA bind to both sides of HOIL-1L-UBL, respectively. The experiments of Gromacs molecular dynamics simulations, SPR and ITC demonstrate that the elongated domain formed by HOIP (629‒695) and HOIP-UBA, hereafter referred to as the HOIP (466‒695) structure, interacts with HOIL-1L-UBL to form a structurally stable complex. These findings illustrate the collaborative interaction between HOIP-UBA and HOIP (629‒695) with HOIL-1L-UBL, which influences the structural stability of LUBAC.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140663752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DPP3 promotes breast cancer tumorigenesis by stabilizing FASN and promoting lipid synthesis. DPP3 通过稳定 FASN 和促进脂质合成来促进乳腺癌的发生。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-24 DOI: 10.3724/abbs.2024054

Xiaoyu Fu, Xu Li, Weixing Wang, Juanjuan Li

{"title":"DPP3 promotes breast cancer tumorigenesis by stabilizing FASN and promoting lipid synthesis.","authors":"Xiaoyu Fu, Xu Li, Weixing Wang, Juanjuan Li","doi":"10.3724/abbs.2024054","DOIUrl":"https://doi.org/10.3724/abbs.2024054","url":null,"abstract":"DPP3, a dipeptidyl peptidase, participates in a variety of pathophysiological processes. DPP3 is upregulated in cancer and might serve as a key factor in the tumorigenesis and progression of various malignancies. However, its specific role and molecular mechanism are still unknown. In this study, the expression of DPP3 in breast cancer tissues is analyzed using TCGA database. Kaplan-Meier survival analysis is performed to estimate the effect of DPP3 on the survival outcomes. To explore the biological function and mechanisms of DPP3 in breast cancer, biochemical and cell biology assays are conducted in vitro. DPP3 expresses at a higher level in breast cancer tissues than that in adjacent tissues in both TCGA database and clinical samples. Patients with high expression of DPP3 have poor survival outcomes. The proliferation and migration abilities of tumor cells with stable DPP3 knockout in breast cancer cell lines are significantly inhibited, and apoptosis is increased in vitro. GSEA analysis shows that DPP3 can affect lipid metabolism and fatty acid synthesis in tumors. Subsequent experiments show that DPP3 could stabilize FASN expression and thus promote fatty acid synthesis in tumor cells. The results of the metabolomic analysis also confirm that DPP3 can affect the content of free fatty acids. This study demonstrates that DPP3 plays a role in the reprogramming of fatty acid metabolism in tumors and is associated with poor prognosis in breast cancer patients. These findings will provide a new therapeutic target for the treatment of breast cancer.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"118 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140659296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of a DNA G-quadruplex causes a gain-of-function SCL45A1 variant relevant to developmental disorders. DNA G-四联体的破坏会导致与发育障碍有关的功能增益型 SCL45A1 变异。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-24 DOI: 10.3724/abbs.2024053

Yuxi Chen, Jiang Long, Sixian Wu, Yazhen Wei, Fei Yan, Qing Li, Jierui Yan, Nannan Zhang, Wenming Xu

{"title":"Disruption of a DNA G-quadruplex causes a gain-of-function SCL45A1 variant relevant to developmental disorders.","authors":"Yuxi Chen, Jiang Long, Sixian Wu, Yazhen Wei, Fei Yan, Qing Li, Jierui Yan, Nannan Zhang, Wenming Xu","doi":"10.3724/abbs.2024053","DOIUrl":"https://doi.org/10.3724/abbs.2024053","url":null,"abstract":"SLC45A1 encodes a glucose transporter protein highly expressed in the brain. Mutations in SLC45A1 may lead to neurological diseases and developmental disorders, but its exact role is poorly understood. DNA G-quadruplexes (DNA G4s) are stable structures formed by four guanine bases and play a role in gene regulation and genomic stability. Changes in DNA G4s may affect brain development and function. The mechanism linking alterations in DNA G-quadruplex structures to SLC45A1 pathogenicity remains unknown. In this study, we identify a functional DNA G-quadruplex and its key binding site on SLC45A1 (NM_001080397.3: exon 2: c.449 G>A: p.R150K). This variant results in the upregulation of mRNA and protein expression, which may lead to intellectual developmental disorder with neuropsychiatric features. Mechanistically, the mutation is found to disrupt DNA G-quadruplex structures on SLC45A1, leading to transcriptional enhancement and a gain-of-function mutation, which further causes increased expression and function of the SLC45A1 protein. The identification of the functional DNA G-quadruplex and its effects on DNA G4s may provide new insights into the genetic basis of SLC45A1 pathogenicity and highlight the importance of DNA G4s of SLC45A1 in regulating gene expression and brain development.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"6 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140663764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated analysis of endometrial stromal cell long noncoding RNA and mRNA expression profiles associated with TGF-β1-induced fibrosis. 与 TGF-β1 诱导的纤维化相关的子宫内膜基质细胞长非编码 RNA 和 mRNA 表达谱的综合分析

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-19 DOI: 10.3724/abbs.2024052

Jianhong Wu, L. Fan, Lin Li, Yudi Zhang, Yucui Tian, Ziwen Jiang, Zhaohui Liu, Dan Lu, Yinmei Dai

引用次数: 0

UBE2C enhances temozolomide resistance by regulating the expression of p53 to induce aerobic glycolysis in glioma. UBE2C 通过调节 p53 的表达来诱导胶质瘤中的有氧糖酵解，从而增强替莫唑胺的耐药性。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-18 DOI: 10.3724/abbs.2024033

Kun Zhou, Dexin Wang, Xiaolin Du, Xia Feng, Xiaoxi Zhu, Cheng Wang

{"title":"UBE2C enhances temozolomide resistance by regulating the expression of p53 to induce aerobic glycolysis in glioma.","authors":"Kun Zhou, Dexin Wang, Xiaolin Du, Xia Feng, Xiaoxi Zhu, Cheng Wang","doi":"10.3724/abbs.2024033","DOIUrl":"https://doi.org/10.3724/abbs.2024033","url":null,"abstract":"UBE2C is overexpressed in gliomas, and its overexpression has been reported to be correlated with the drug resistance of gliomas to some extent. In this study, we explore the role of UBE2C in regulating temozolomide (TMZ) resistance in glioma and investigate the underlying mechanisms involved. Twenty normal brain tissues and 100 glioma tissues from 50 TMZ-resistant patients and 50 TMZ-sensitive patients are included in this study. TMZ-resistant cell lines are constructed to explore the role of UBE2C in regulating glioma cell viability and TMZ resistance. Our results show that both the mRNA and protein levels of UBE2C are significantly elevated in the brain tissues of glioma patients, especially in those of TMZ-resistant patients. Consistently, UBE2C expression is markedly upregulated in TMZ-resistant cell lines. Overexpression of UBE2C rescues glioma cells from TMZ-mediated apoptosis and enhances cell viability. In contrast, downregulation of UBE2C expression further enhances TMZ function, increases cell apoptosis and decreases cell viability. Mechanistically, UBE2C overexpression decreases p53 expression and enhances aerobic glycolysis level by increasing ATP level, lactate production, and glucose uptake. Downregulation of p53 level abolishes the role of UBE2C downregulation in inhibiting TMZ resistance and aerobic glycolysis in glioma cells. Moreover, an animal assay confirms that downregulation of UBE2C expression further suppresses tumor growth in the context of TMZ treatment. Collectively, this study reveals that downregulation of UBE2C expression enhances the sensitivity of glioma cells to TMZ by regulating the expression of p53 to inhibit aerobic glycolysis.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140687315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lycium barbarum polysaccharide ameliorates the accumulation of lipid droplets in adipose tissue via an ATF6/SIRT1-dependent mechanism. 枸杞多糖通过 ATF6/SIRT1 依赖性机制改善脂肪组织中脂滴的积聚。

Acta Biochimica et Biophysica Sinica Pub Date : 2024-04-12 DOI: 10.3724/abbs.2024046

Rui Zhou, Yajing Liu, Weiqiang Hu, Jing Yang, Bing Lin, Zhentian Zhang, Mingyan Chen, Jingwen Yi, Cuifeng Zhu

{"title":"Lycium barbarum polysaccharide ameliorates the accumulation of lipid droplets in adipose tissue via an ATF6/SIRT1-dependent mechanism.","authors":"Rui Zhou, Yajing Liu, Weiqiang Hu, Jing Yang, Bing Lin, Zhentian Zhang, Mingyan Chen, Jingwen Yi, Cuifeng Zhu","doi":"10.3724/abbs.2024046","DOIUrl":"https://doi.org/10.3724/abbs.2024046","url":null,"abstract":"Lipid droplets (LDs) are dynamic organelles that store neutral lipids and are closely linked to obesity. Previous studies have suggested that Lycium barbarum polysaccharide (LBP) supplements can ameliorate obesity, but the underlying mechanisms remain unclear. In this study, we hypothesize that LBP alleviates LD accumulation in adipose tissue (AT) by inhibiting fat-specific protein 27 (Fsp27) through an activating transcription factor-6 (ATF6)/small-molecule sirtuin 1 (SIRT1)-dependent mechanism. LD accumulation in AT is induced in high-fat diet (HFD)-fed mice, and differentiation of 3T3-L1 preadipocytes (PAs) is induced. The ability of LBP to alleviate LD accumulation and the possible underlying mechanism are then investigated both in vivo and in vitro. The influences of LBP on the expressions of LD-associated genes ( ATF6 and Fsp27) are also detected. The results show that HFD and PA differentiation markedly increase LD accumulation in ATs and adipocytes, respectively, and these effects are markedly suppressed by LBP supplementation. Furthermore, LBP significantly activates SIRT1 and decreases ATF6 and Fsp27 expressions. Interestingly, the inhibitory effects of LBP are either abolished or exacerbated when ATF6 is overexpressed or silenced, respectively. Furthermore, SIRT1 level is transcriptionally regulated by LBP through opposite actions mediated by ATF6. Collectively, our findings suggest that LBP supplementation alleviates obesity by ameliorating LD accumulation, which might be partially mediated by an ATF6/SIRT1-dependent mechanism.","PeriodicalId":503337,"journal":{"name":"Acta Biochimica et Biophysica Sinica","volume":"79 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140710984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0