International Journal of Developmental Biology最新文献_第9页

Molecular characterization of the prostaglandin E receptor subtypes 2a and 4b and their expression patterns during embryogenesis in zebrafish. 斑马鱼胚胎发生过程中前列腺素E受体2a和4b亚型的分子特征及其表达模式

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.210003wh

Yongjun Han, Hongbo Chang, Hong Wu

{"title":"Molecular characterization of the prostaglandin E receptor subtypes 2a and 4b and their expression patterns during embryogenesis in zebrafish.","authors":"Yongjun Han, Hongbo Chang, Hong Wu","doi":"10.1387/ijdb.210003wh","DOIUrl":"https://doi.org/10.1387/ijdb.210003wh","url":null,"abstract":"The molecular expression profiles of zebrafish ep2a and ep4b have not been defined to date. Phylogenetic trees of EP2a and EP4b in zebrafish and other species revealed that human EP4 and zebrafish EP4b were more closely related than EP2a. Zebrafish EP2a is a 281 amino acid protein which shares high identity with that of human (43%), mouse (44%), rat (43%), dog (44%), cattle (41%), and chicken (41%). Zebrafish EP4b encoded a 497 amino acid precursor with high amino acid identity to that of mammals, including human (57%), mouse (54%), rat (55%), dog (55%), cattle (56%), and chicken (54%). Whole-mount in situ hybridization revealed that ep2a was robustly expressed in the anterior four somites at the 10-somites stages, but was absent in the somites at 19 hpf. It was observed again in the pronephric duct at 24 hpf, in the intermediate cell mass located in the trunk, and in the rostral blood island at 30 hpf. Ep2a was also expressed in the notochord at 48 hpf. During somitogenesis, ep4b was highly expressed in the eyes, somites, and the trunk neural crest. From 30 to 48 hpf, ep4b could be detected in the posterior cardinal vein and the neighboring inner cell mass. From these data we conclude that ep2a and ep4b are conserved in vertebrates and that the presence of ep2a and ep4b transcripts during developmental stages infers their role during early zebrafish larval development. In addition, the variable expression of the two receptor isoforms was strongly suggestive of divergent roles of molecular regulation.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39437354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells. SV40T将雪旺细胞重编程为干细胞样细胞，可以重新分化为末梢神经细胞。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.210062zz

Rui-Fang Li, Guo-Xin Nan, Dan Wang, Chang Gao, Juan Yang, Tong-Chuan He, Zhong-Lin Zhang

{"title":"SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells.","authors":"Rui-Fang Li, Guo-Xin Nan, Dan Wang, Chang Gao, Juan Yang, Tong-Chuan He, Zhong-Lin Zhang","doi":"10.1387/ijdb.210062zz","DOIUrl":"https://doi.org/10.1387/ijdb.210062zz","url":null,"abstract":"Background: The specific effect of SV40T on neurocytes has seldom been investigated by the researchers. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T, in order to explore the effects of SV40T on Schwann cells.Methods: SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, and also co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry.Results: SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus. In addition, SV40T up-regulated the expressions of neural crest-associated markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the markers of mature SCs MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells.Conclusions: SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells (NCCs) that can differentiate to neurocytes.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39706103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UNESCO Chair of Developmental Biology: how an initiative that fostered careers in Developmental Biology impacted Brazilian Science. 教科文组织发育生物学教席:一项促进发育生物学职业发展的倡议如何影响巴西科学。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200132ja

Jose G Abreu

{"title":"UNESCO Chair of Developmental Biology: how an initiative that fostered careers in Developmental Biology impacted Brazilian Science.","authors":"Jose G Abreu","doi":"10.1387/ijdb.200132ja","DOIUrl":"https://doi.org/10.1387/ijdb.200132ja","url":null,"abstract":"The UNESCO Chair in Developmental Biology started in 1998, at the Institute of Biomedical Sciences of the Federal University of Rio de Janeiro, in Brazil. This Chair was a Brazilian-French initiative led by Professor Vivaldo Moura Neto and Professor Nicole Le Douarin, one of the most inspiring Developmental Biologists of the 20th and 21st centuries. The UNESCO Chair wanted to stimulate interest in Developmental Biology among Brazilian students and scientists by organizing annual international courses on Evolution and Developmental Biology at an advanced level. At the Federal University of Rio de Janeiro, the UNESCO Chair established an international laboratory for the permanent training of researchers and the development of research programs in Developmental Biology and related areas. Moreover, the program aimed at establishing an international network connecting Brazilian Universities and research centers in Latin America and Europe. The advanced hands-on courses, symposiums, and workshops promoted by this Chair inspired the careers of many young scientists. They generated new lines of research in Developmental Biology using a variety of animal models. This review does not intend to bring up all the historical events that marked the beginning of Developmental Biology in Brazil. Instead, it will be dedicated to highlighting one specific initiative that inspired a new generation of Developmental Biologists who established important research lines and contributed to the advance of this scientific field in Brazil.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38380165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing embryonic territories in the context of Wnt signaling. 在Wnt信号的背景下建立胚胎领地。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200231ja

Ian Velloso, Lorena A Maia, Nathalia G Amado, Alice H Reis, Xi He, Jose G Abreu

{"title":"Establishing embryonic territories in the context of Wnt signaling.","authors":"Ian Velloso, Lorena A Maia, Nathalia G Amado, Alice H Reis, Xi He, Jose G Abreu","doi":"10.1387/ijdb.200231ja","DOIUrl":"https://doi.org/10.1387/ijdb.200231ja","url":null,"abstract":"This review highlights the work that my research group has been developing, together with international collaborators, during the last decade. Since we were able to establish the Xenopus laevis experimental model in Brazil, we have been focused on understanding early embryonic patterns regarding neural induction and axes establishment. In this context, the Wnt pathway appears as a major player and has been much explored by us and other research groups. Here, we chose to review three published works which we consider to be landmarks within the course of our research and also within the history of modern findings regarding neural induction and patterning. We intend to show how our series of discoveries, when painted together, tells a story that covers crucial developmental windows of early differentiation paths of anterior neural tissue: 1. establishing the head organizer in contrast to the trunk organizer in the early gastrula; 2. deciding between neural ectoderm and epidermis ectoderm at the blastula/gastrula stages, and 3. the gathering of prechordal unique properties in the late gastrula/early neurula.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38380169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress and sex determination in fish: from brain to gonads. 鱼类的压力和性别决定:从大脑到性腺。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200072jf

Diana C Castañeda-Cortés, Juan I Fernandino

{"title":"Stress and sex determination in fish: from brain to gonads.","authors":"Diana C Castañeda-Cortés, Juan I Fernandino","doi":"10.1387/ijdb.200072jf","DOIUrl":"https://doi.org/10.1387/ijdb.200072jf","url":null,"abstract":"Fish present remarkable malleability regarding gonadal sex fate. This phenotypic plasticity enables an organism to adapt to changes in the environment by responding with different phenotypes. The gonad and the brain present this extraordinary plasticity. These organs are involved in the response to environmental stressors to direct gonadal fate, inducing sex change or sex reversal in hermaphroditic and gonochoristic fish, respectively. The presence of such molecular and endocrine plasticity gives this group a large repertoire of possibilities against a continuously changing environment, resulting in the highest radiation of reproduction strategies described in vertebrates. In this review, we provide a broad and comparative view of tremendous radiation of sex determination mechanisms to direct gonadal fate. New results have established that the driving mechanism involves early response to environmental stressors by the brain plus high plasticity of gonadal differentiation and androgens as by-products of stress inactivation. In addition to the stress axis, two other major axes - the hypothalamic-pituitary-gonadal axis and the hypothalamic-pituitary-thyroid axis, which are well known for their participation in the regulation of reproduction - have been proposed to reinforce brain-gonadal interrelationships in the fate of the gonad.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.200072jf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38380586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Oxygen, reactive oxygen species and developmental redox networks: Evo-Devo Evil-Devils? 氧，活性氧和发育氧化还原网络:进化-发展-恶魔?

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200170es

Enrique Salas-Vidal, Francisco J Méndez-Cruz, Arlen Ramírez-Corona, Brenda Reza-Medina

引用次数: 0

20 years of the "Practical Course in Developmental Biology" in Latin America: from Santiago to Quintay, via Juquehy, Buenos Aires and Montevideo. 拉丁美洲“发育生物学实践课程”20年:从圣地亚哥到昆塔伊，途经Juquehy、布宜诺斯艾利斯和蒙得维的亚。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200049rm

Roberto Mayor

引用次数: 0

Roles of cytochromes P450 in plant reproductive development. 细胞色素P450在植物生殖发育中的作用。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200100gp

Ayelén M Distéfano, Nicolás Setzes, Milagros Cascallares, Diego F Fiol, Eduardo Zabaleta, Gabriela C Pagnussat

引用次数: 6

Genomics and epigenomics of axolotl regeneration. 蝾螈再生的基因组学和表观基因组学。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200276cs

Cynthia Sámano, Rodrigo González-Barrios, Mariana Castro-Azpíroz, Daniela Torres-García, José A Ocampo-Cervantes, Jimena Otero-Negrete, Ernesto Soto-Reyes

{"title":"Genomics and epigenomics of axolotl regeneration.","authors":"Cynthia Sámano, Rodrigo González-Barrios, Mariana Castro-Azpíroz, Daniela Torres-García, José A Ocampo-Cervantes, Jimena Otero-Negrete, Ernesto Soto-Reyes","doi":"10.1387/ijdb.200276cs","DOIUrl":"https://doi.org/10.1387/ijdb.200276cs","url":null,"abstract":"The axolotl (Ambystoma mexicanum) has been a widely studied organism due to its capacity to regenerate most of its cells, tissues and whole-body parts. Since its genome was sequenced, several molecular tools have been developed to study the mechanisms behind this outstanding and extraordinary ability. The complexity of its genome due to its sheer size and the disproportionate expansion of a large number of repetitive elements, may be a key factor at play during tissue remodeling and regeneration mechanisms. Transcriptomic analysis has provided information to identify candidate genes networks and pathways that might define successful or failed tissue regeneration. Nevertheless, the epigenetic machinery that may participate in this phenomenon has largely not been studied. In this review, we outline a broad overview of both genetic and epigenetic molecular processes related to regeneration in axolotl, from the macroscopic to the molecular level. We also explore the epigenetic mechanisms behind regenerative pathways, and its potential importance in future regeneration research. Altogether, understanding the genomics and global regulation in axolotl will be key for elucidating the special biology of this organism and the fantastic phenomenon that is regeneration.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25409435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Early and late stages of Developmental Biology in Argentina. 阿根廷发育生物学的早期和晚期。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2021-01-01 DOI: 10.1387/ijdb.200024ss

Sara S Sánchez, Stella M Honoré

{"title":"Early and late stages of Developmental Biology in Argentina.","authors":"Sara S Sánchez, Stella M Honoré","doi":"10.1387/ijdb.200024ss","DOIUrl":"https://doi.org/10.1387/ijdb.200024ss","url":null,"abstract":"The history of science in Argentina is based on the enormous contribution that the great immigration of the 19th and 20th centuries produced in the country. The scientific and philosophical ideas and the role played especially by Italian scientists who arrived in the country produced a great impact on the different disciplines including Development Biology in emerging universities. The University of Tucumán pioneered the study of experimental biology, making important contributions to reproductive biology and to the early development of amphibians. The contribution of the Italian embryologist Armando Pisanó and the Argentinian Francisco D. Barbieri expanded the field to other universities and research centers located in Córdoba, La Plata, Bahía Blanca and Rosario. Given its strategic position, laboratories located in the city of Buenos Aires reached technological advances faster than others. Indeed, these laboratories saw the evolution from experimental biology to developmental genetics, renewing interest in this area. Currently, Developmental Biology brings together young researchers eager to consolidate regional and global collaboration networks that seek to help solve specific problems such as fertility, epigenetics, stem cells and tissue engineering.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38477959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0