Plant & Cell Physiology最新文献

Evolutionary Systems Biology Identifies Genetic Trade-offs In Rice Defense Against Above- and Belowground Attackers 进化系统生物学发现了水稻防御地上和地下攻击者的遗传权衡因素

Plant & Cell Physiology Pub Date : 2024-09-18 DOI: 10.1093/pcp/pcae107

Taryn S Dunivant, Damaris Godinez-Vidal, Craig Perkins, Madelyn G Lee, Matthew Ta, Simon C Groen

{"title":"Evolutionary Systems Biology Identifies Genetic Trade-offs In Rice Defense Against Above- and Belowground Attackers","authors":"Taryn S Dunivant, Damaris Godinez-Vidal, Craig Perkins, Madelyn G Lee, Matthew Ta, Simon C Groen","doi":"10.1093/pcp/pcae107","DOIUrl":"https://doi.org/10.1093/pcp/pcae107","url":null,"abstract":"Like other plants, wild and domesticated rice species (Oryza nivara, O. rufipogon, and O. sativa) evolve in environments with various biotic and abiotic stresses that fluctuate in intensity through space and time. Microbial pathogens and invertebrate herbivores such as plant-parasitic nematodes and caterpillars show geographical and temporal variation in activity patterns and may respond differently to certain plant defensive mechanisms. As such, plant interactions with multiple community members may result in conflicting selection pressures on genetic polymorphisms. Here, through assays with different above- and belowground herbivores, the fall armyworm (Spodoptera frugiperda) and the southern root-knot nematode (Meloidogyne incognita), respectively, and comparison with rice responses to microbial pathogens, we identify potential genetic trade-offs at the KSL8 and MG1 loci on chromosome 11. KSL8 encodes the first committed step towards biosynthesis of either stemarane- or stemodane-type diterpenoids through the japonica (KSL8-jap) or indica (KSL8-ind) allele. Knocking out KSL8-jap and CPS4, encoding an enzyme that acts upstream in diterpenoid synthesis, in japonica rice cultivars increased resistance to S. frugiperda and decreased resistance to M. incognita. Furthermore, MG1 resides in a haplotype that provided resistance to M. incognita, while alternative haplotypes are involved in mediating resistance to the rice blast fungus Magnaporthe oryzae and other pests and pathogens. Finally, KSL8 and MG1 alleles are located within trans-species haplotypes and may be evolving under long-term balancing selection. Our data are consistent with a hypothesis that polymorphisms at KSL8 and MG1 may be maintained through complex and diffuse community interactions.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dominant-negative KAI2d paralogs putatively attenuate strigolactone responses in root parasitic plants 显性阴性 KAI2d 准同族体可能会减弱根寄生植物对绞股蓝内酯的反应

Plant & Cell Physiology Pub Date : 2024-09-14 DOI: 10.1093/pcp/pcae106

Alexandra R.F White, Annalise Kane, Satoshi Ogawa, Ken Shirasu, David C Nelson

{"title":"Dominant-negative KAI2d paralogs putatively attenuate strigolactone responses in root parasitic plants","authors":"Alexandra R.F White, Annalise Kane, Satoshi Ogawa, Ken Shirasu, David C Nelson","doi":"10.1093/pcp/pcae106","DOIUrl":"https://doi.org/10.1093/pcp/pcae106","url":null,"abstract":"Many root parasitic plants in the Orobanchaceae use host-derived strigolactones as germination cues. This adaptation facilitates attachment to a host and is particularly important for the success of obligate parasitic weeds that cause substantial crop losses globally. Parasite seeds sense strigolactones through “divergent” KARRIKIN INSENSITIVE2 (KAI2d)/HYPOSENSITIVE TO LIGHT (HTL) α/β-hydrolases that have undergone substantial duplication and diversification in Orobanchaceae genomes. After germination, chemotropic growth of parasite roots toward a strigolactone source also occurs in some species. We investigated which of the seven KAI2d genes found in a facultative hemiparasite, Phtheirospermum japonicum, may enable chemotropic responses to strigolactones. To do so, we developed a triple mutant Nbd14a,b kai2i line of Nicotiana benthamiana in which strigolactone-induced degradation of SMAX1, an immediate downstream target of KAI2 signaling, is disrupted. In combination with a transiently expressed, ratiometric reporter of SMAX1 protein abundance, this mutant forms a system for the functional analysis of parasite KAI2d proteins in a plant cellular context. Using this system, we unexpectedly found three PjKAI2d proteins that do not trigger SMAX1 degradation in the presence of strigolactones. Instead, these PjKAI2d inhibit the perception of low strigolactone concentrations by strigolactone-responsive PjKAI2d in a dominant-negative manner that depends upon an active catalytic triad. Similar dominant-negative KAI2d paralogs were identified in an obligate hemiparasitic weed, Striga hermonthica. These proteins suggest a mechanism for attenuating strigolactone signaling in parasites, which might be used to enhance the perception of shallow strigolactone gradients during root growth toward a host or to restrict germination responses to specific strigolactones.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Sesquiterpenoid Production by Methyl Jasmonate in Atractylodes chinensis Adventitious Root Culture and its Transcriptional Regulation 茉莉酸甲酯对白术不定根培养中倍半萜类化合物生产的促进作用及其转录调控

Plant & Cell Physiology Pub Date : 2024-09-14 DOI: 10.1093/pcp/pcae104

Cholil Yun, Solme Pak, Yulin Pang, Jong Min Phyon, Liuyang Yao, Xiaorui Guo, Cholung Kim, Songsu Ma, Tongchol Ri, Ryongho Pak, Huimei Wang

{"title":"Enhancement of Sesquiterpenoid Production by Methyl Jasmonate in Atractylodes chinensis Adventitious Root Culture and its Transcriptional Regulation","authors":"Cholil Yun, Solme Pak, Yulin Pang, Jong Min Phyon, Liuyang Yao, Xiaorui Guo, Cholung Kim, Songsu Ma, Tongchol Ri, Ryongho Pak, Huimei Wang","doi":"10.1093/pcp/pcae104","DOIUrl":"https://doi.org/10.1093/pcp/pcae104","url":null,"abstract":"The adventitious root (AR) culture of Atractylodes chinensis is an efficient platform for sustainable production of its sesquiterpenoid compounds (atractylon and β-eudesmol). However, their limited accumulation levels need an effective elicitation approach, and the present study solved this problem using methyl jasmonate (MeJA) as an elicitor. The effects of its treatment concentration and duration on metabolite production were investigated. The ARs treated with 100 µM MeJA for seven days increased atractylon and β-eudesmol by 3.64- and 1.90-fold, respectively, compared with the control. This study further performed transcriptome analysis to explore the transcriptional regulation mechanism of the MeJA elicitation. A total of 124,464 unigenes were identified in A. chinensis ARs, of which 3,568 genes were upregulated and 3,864 genes were downregulated under the MeJA treatment. The MeJA treatment activated the endogenous JA biosynthesis and signaling pathways and sesquiterpenoid biosynthesis. The MeJA treatment more significantly activated the MEP pathway than the MVA pathway. In addition, 14 genes encoding terpene synthase were identified to be significantly upregulated. A total of 2,700 transcription factors (TFs) were identified in A. chinensis ARs, of which Tify, MYB, and MADS were significantly enriched under the MeJA treatment. We predicted a new antagonistic interaction between MYC2 and CPP TFs, which was significantly regulated by the MeJA treatment. The results of real-time quantitative PCR and enzyme activity assays proved the reliability of the transcriptome data. This study will help improve the in vitro production system of A. chinensis sesquiterpenoids and understand the transcriptional regulation mechanism of MeJA elicitation.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

At2-MMP is required for attenuation of cell proliferation during wound healing in incised Arabidopsis inflorescence stems 拟南芥花序茎切口愈合过程中的细胞增殖需要 At2-MMP 的参与

Plant & Cell Physiology Pub Date : 2024-09-14 DOI: 10.1093/pcp/pcae103

Afiifah Machfuudzoh, Weerasak Pitaksaringkarn, Ryo Koshiba, Takumi Higaki, Randeep Rakwal, Yusuke Ohba, Masashi Asahina, Shinobu Satoh, Hiroaki Iwai

{"title":"At2-MMP is required for attenuation of cell proliferation during wound healing in incised Arabidopsis inflorescence stems","authors":"Afiifah Machfuudzoh, Weerasak Pitaksaringkarn, Ryo Koshiba, Takumi Higaki, Randeep Rakwal, Yusuke Ohba, Masashi Asahina, Shinobu Satoh, Hiroaki Iwai","doi":"10.1093/pcp/pcae103","DOIUrl":"https://doi.org/10.1093/pcp/pcae103","url":null,"abstract":"Wound healing of partially incised Arabidopsis inflorescence stems constitutes cell proliferation that initiates mainly in pith tissues about three days after incision, and that the healing process completes in about seven days. Although the initiation mechanisms of cell proliferation have been well documented, the suppression mechanisms remain elusive. Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases well-known as proteolytic enzymes in animal systems functioning in extracellular matrix remodeling during physiological and pathological processes, including tissue differentiation, growth, defense, wound healing, and control of cancer growth. In this study, we report At2-MMP might contribute to the suppression mechanism of cell proliferation during tissue-repair process of incised inflorescence stems. At2-MMP transcript was gradually upregulated from day 0 to 5 after incision, and slightly decreased on day 7. Morphological analysis of incised stem of defected mutant at2-mmp revealed significantly enhanced cell proliferation around the incision site. Consistent with this, semi-quantitative analysis of dividing cells displayed a significant increment in the number of dividing cells in at2-mmp as compared to WT. These results showed that the upregulation of At2-MMP at the later stage of wound-healing process is likely to be involved in the completion of the process by attenuating the cell proliferation.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive strigolactone perception by a divergent clade KAI2 receptor in a facultative root parasitic plant, Phtheirospermum japonicum 一种兼性根寄生植物 Phtheirospermum japonicum 的分化支系 KAI2 受体对绞股蓝内酯的高灵敏度感知

Plant & Cell Physiology Pub Date : 2024-09-14 DOI: 10.1093/pcp/pcae105

Saori Takei, Masahiko Otani, Tomoya Ishikawa, Taiki Suzuki, Shoma Okabe, Kotaro Nishiyama, Naoto Kawakami, Yoshiya Seto

{"title":"Highly sensitive strigolactone perception by a divergent clade KAI2 receptor in a facultative root parasitic plant, Phtheirospermum japonicum","authors":"Saori Takei, Masahiko Otani, Tomoya Ishikawa, Taiki Suzuki, Shoma Okabe, Kotaro Nishiyama, Naoto Kawakami, Yoshiya Seto","doi":"10.1093/pcp/pcae105","DOIUrl":"https://doi.org/10.1093/pcp/pcae105","url":null,"abstract":"Phtheirospermum japonicum, a member of the Orobanchaceae family, is a facultative root parasitic plant that can survive without parasitizing the host. In contrast, obligate root parasitic plants such as Striga and Orobanche, which are also members of the Orobanchaceae family, cannot survive in the absence of the host. The germination of obligate root parasitic plants is typically induced by host root-derived strigolactones (SLs) at very low concentrations. The KAI2/HTL family proteins have been found to be involved in the perception of karrikin (KAR), a smoke-derived germination inducer and unidentified endogenous ligand, in non-parasitic plants. Obligate root parasitic plants possess uniquely diverged KAI2 clade genes, which are collectively referred to as KAI2d. Many of those have been shown to function as SL receptors. Intriguingly, the KAI2d clade genes are also conserved in P. japonicum, even though this plant does not require SLs for germination. The biochemical and physiological functions of the KAI2d proteins in P. japonicum remain unclear. Here, we report that some of these proteins can function as SL receptors in P. japonicum. Moreover, we found that one of them, PjKAI2d4, is highly sensitive to SLs when expressed in Arabidopsis, and it is similar to the sensitive SL receptors found in Striga and Orobanche. These results suggest that the KAI2d clade SL receptors play a crucial role not only in obligate parasites but also in facultative parasitic plants.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genetics of plant-plant interactions and their cascading effects on agroecosystem – from model plants to application 植物与植物之间相互作用的遗传学及其对农业生态系统的连锁效应--从模式植物到应用

Plant & Cell Physiology Pub Date : 2024-09-10 DOI: 10.1093/pcp/pcae092

Yasuhiro Sato, Wuest Samuel E

{"title":"The genetics of plant-plant interactions and their cascading effects on agroecosystem – from model plants to application","authors":"Yasuhiro Sato, Wuest Samuel E","doi":"10.1093/pcp/pcae092","DOIUrl":"https://doi.org/10.1093/pcp/pcae092","url":null,"abstract":"Plants are typically surrounded by neighboring individuals in agricultural fields or natural environments. In such circumstances, plant-plant interactions (PPI) are ubiquitous and represent not only important evolutionary forces but also have consequences on (agro-)ecosystem functioning, such as increased productivity and resistance. However, many mechanisms underlying these PPIs remain poorly understood. Recently, new genomic technologies and tools, such as genome-wide association studies, have facilitated genetic approaches to study PPIs, particularly among conspecific individuals. Here, we highlight emerging issues and advances in the field by focusing on three different aspects. First, we overview the current status of genetic mapping studies on PPIs and pinpoint that these studies open a new opportunity that is relevant to agriculture and breeding. Second, we introduce two proof-of-concept studies in Arabidopsis thaliana, in which genetic differences among plants improved the functioning of genotype mixtures. Both studies were able to predict effective mixtures with different experimental designs applied to different outcomes (productivity increase vs. herbivory mitigation). Third, we discuss implications from these case studies about how PPIs have cascading effects that propagate to higher levels of biological organization, such as populations or communities. At the population level, plant yield or resistance can be optimized through breeding that achieves reduced competition or push-pull protection systems, respectively. At the community level, these population-level changes may have further beneficial effects on plant-associated organisms. Overall, we suggest that the increasing availability of genomic resources will improve our understanding of PPIs and thereby contribute to the management of crops or semi-natural ecosystems.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromorphological and chemical characterization of Drimys winteri leaf surfaces: The secondary alcohols forming epicuticular wax crystals are accompanied by alkanediol, alkanetriol and ketol derivatives. Drimys winteri 叶子表面的微观形态和化学特征：烷二醇、烷三醇和酮醇衍生物是形成表皮蜡晶体的仲醇。

Plant & Cell Physiology Pub Date : 2024-05-17 DOI: 10.1093/pcp/pcae053

Zhonghang Zhang, Dwiti Mistry, Reinhard Jetter

{"title":"Micromorphological and chemical characterization of Drimys winteri leaf surfaces: The secondary alcohols forming epicuticular wax crystals are accompanied by alkanediol, alkanetriol and ketol derivatives.","authors":"Zhonghang Zhang, Dwiti Mistry, Reinhard Jetter","doi":"10.1093/pcp/pcae053","DOIUrl":"https://doi.org/10.1093/pcp/pcae053","url":null,"abstract":"The cuticle is a hydrophobic coating of most aerial plant surfaces crucial for limiting non-stomatal water loss. Plant cuticles consist of the lipid polyester cutin and associated waxes with compositions varying widely between plant species and organs. Here, we aimed to provide a comparative analysis of the dark-glossy adaxial and pale-glaucous abaxial sides of Drimys winteri (Winteraceae) leaves. Scanning electron microscopy showed nanotubular wax crystals lining the entire abaxial side of the leaf (including stomatal pores), while the adaxial side had patches of mixed platelet/tubule crystals and smooth areas between them. Consecutive treatments for wax removal and cutin depolymerization revealed that the waxes were deposited on a cutin network with micron-scale cavities across the entire abaxial surface including the stomata pores, and on a microscopically smooth cutin surface on the adaxial side of the leaf. Gas chromatography coupled to mass spectrometry and flame ionization detection showed that the wax mixtures on both sides of the leaf were complex mixtures of very-long-chain compounds dominated by the secondary alcohol nonacosan-10-ol and alkanediols with one hydroxyl on C-10. It is therefore very likely that the tubular wax crystals characteristic of both leaf sides are formed by these alcohols and diols. Further secondary alcohols and alkanediols, as well as ketols and alkanetriols with one functional group on C-10 were identified based on mass spectral fragmentation patterns. The similarities between all these mid-chain functionalized compounds suggest that they are derived from nonacosan-10-ol via regio-specific hydroxylation reactions, likely catalyzed by three P450-dependent monooxygenases with different regio-specificities.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"83 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140964444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Title: Bona Fide Plant Steroid Receptors Are Innovated in Seed Plants and Angiosperms through Successive Whole Genome Duplication Events. 标题：真正的植物类固醇受体通过连续的全基因组复制事件在种子植物和被子植物中得到创新

Plant & Cell Physiology Pub Date : 2024-05-17 DOI: 10.1093/pcp/pcae054

Jing Liu, Qiang Wei, Zhen Zhao, Fanqi Qiang, Guishuang Li, Guang Wu

{"title":"Title: Bona Fide Plant Steroid Receptors Are Innovated in Seed Plants and Angiosperms through Successive Whole Genome Duplication Events.","authors":"Jing Liu, Qiang Wei, Zhen Zhao, Fanqi Qiang, Guishuang Li, Guang Wu","doi":"10.1093/pcp/pcae054","DOIUrl":"https://doi.org/10.1093/pcp/pcae054","url":null,"abstract":"Whole genome duplication (WGD) events are widespread in plants and animals, thus their long-term evolutionary contribution has long been speculated, yet a specific contribution is difficult to verify. Here, we show that ɛ-WGD and ζ-WGD contribute to the origin and evolution of bona fide brassinosteroid (BR) signaling through the innovation of active BR biosynthetic enzymes and active BR receptors from their respective ancestors. We found that BR receptors BRI1 (BR Insensitive 1) and BRL1/3 (BRI1-likes 1/3) derived by ɛ-WGD and ζ-WGD, which occurred in the common ancestor of angiosperms and seed plants, respectively, while orphan BR receptor BRL2 first appeared in stomatophytes. Additionally, CYP85A enzymes synthesizing the bioactive BRs derived from a common ancestor of seed plants while its sister enzymes CYP90 synthesizing BR precursors presented in all land plants, implying possible ligand-receptor coevolution. Consistently, the island domains (IDs) responsible for BR perception in BR receptors were most divergent among different receptor branches, supporting ligand-driven evolution. As a result, BRI1 was the most diversified BR receptor in angiosperms. Importantly, relative to the BR biosynthetic DET2 gene presented in all land plants, BRL2, BRL1/3 and BRI1 had high expression in vascular plants ferns, gymnosperms and angiosperms, respectively. Notably, BRI1 is the most diversified BR receptor with the most abundant expression in angiosperms, suggesting potential positive selection. Therefore, WGDs initiate a neofunctionalization process diverged by ligand-perception and transcriptional expression, which might optimize both BR biosynthetic enzymes and BR receptors, likely contributing to the evolution of land plants, especially seed plants and angiosperms.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140963371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magic in the Bacterial Genome: Shuffling the Genome for N2-Fixation. 细菌基因组中的魔法：重组基因组实现 N2 固定。

Plant & Cell Physiology Pub Date : 2024-05-17 DOI: 10.1093/pcp/pcae052

Haruhiko Jimbo

引用次数: 0

Rice SRO1a contributes to Xanthomonas TAL effector-mediated expression of host susceptible gene. 水稻 SRO1a 有助于黄单胞菌 TAL 效应器介导的宿主易感基因的表达。

Plant & Cell Physiology Pub Date : 2024-05-17 DOI: 10.1093/pcp/pcae057

S. Yoshimura, Ayaka Yoshihisa, Yusei Okamoto, Haruna Hirano, Yuina Nakai, K. Yamaguchi, Tsutomu Kawasaki

{"title":"Rice SRO1a contributes to Xanthomonas TAL effector-mediated expression of host susceptible gene.","authors":"S. Yoshimura, Ayaka Yoshihisa, Yusei Okamoto, Haruna Hirano, Yuina Nakai, K. Yamaguchi, Tsutomu Kawasaki","doi":"10.1093/pcp/pcae057","DOIUrl":"https://doi.org/10.1093/pcp/pcae057","url":null,"abstract":"Xanthomonas species infect many important crops and cause huge yield loss. These pathogens deliver transcription activator-like (TAL) effectors into the cytoplasm of plant cells. TAL effectors move to host nuclei, directly bind to the promoters of host susceptible genes, and activate their transcription. However, the molecular mechanisms by which TAL effectors induce host transcription remain unclear. We herein demonstrated that TAL effectors interacted with the SIMILAR TO RCD ONE (SRO) family proteins OsSRO1a and OsSRO1b in nuclei. A transactivation assay using rice protoplasts indicated that OsSRO1a and OsSRO1b enhanced the activation of the OsSWEET14 promoter by the TAL effector AvrXa7. The AvrXa7-mediated expression of OsSWEET14 was significantly reduced in ossro1a mutants. However, the overexpression of OsSRO1a increased disease resistance by up-regulating the expression of defense-related genes, such as WRKY62 and PBZ1. This was attributed to OsSRO1a and OsSRO1b also enhancing the transcriptional activity of WRKY45, a direct regulator of WRKY62 expression. Therefore, OsSRO1a and OsSRO1b appear to positively contribute to transcription mediated by bacterial TAL effectors and rice transcription factors.","PeriodicalId":502140,"journal":{"name":"Plant & Cell Physiology","volume":"26 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140965107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0