Journal of Environmental Pathology Toxicology and Oncology最新文献_第9页

Hypoxia Induces Overexpression of CCL28 to Recruit Treg Cells to Enhance Angiogenesis in Lung Adenocarcinoma. 缺氧诱导CCL28过表达募集Treg细胞促进肺腺癌血管生成

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2020035859

Bingchun Liu, Chunyan Wei

引用次数: 14

Nuclear Pore Complex 62 Promotes Metastasis of Gastric Cancer by Regulating Wnt/β-Catenin and TGF-β Signaling Pathways. 核孔复合物62通过调节Wnt/β-Catenin和TGF-β信号通路促进胃癌转移

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021037136

Hua Wang, Yajing Lin, Jingpeng Jin, Hongxing Shen, Chun Dai

{"title":"Nuclear Pore Complex 62 Promotes Metastasis of Gastric Cancer by Regulating Wnt/β-Catenin and TGF-β Signaling Pathways.","authors":"Hua Wang, Yajing Lin, Jingpeng Jin, Hongxing Shen, Chun Dai","doi":"10.1615/JEnvironPatholToxicolOncol.2021037136","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021037136","url":null,"abstract":"Gastric cancer (GC) is the third leading cause of cancer-related deaths in the world. Tumor metastasis is considered one of the main factors for GC development. Nup62 is a member of the nuclear pore complex (NPC). It bridges the nuclear envelope, is important in nucleocytoplasmic exchange, and is associated with cancer. This study aimed to investigate the role of Nup62 in GC metastasis. The relationship between the expression level of Nup62 in GC and patient survival was evaluated using Kaplan-Meier analysis. Then Nup62 expression in GC tissues and matched normal gastric tissues was analyzed by immunohistochemistry and that in cell lines by Western blot analysis. Furthermore, clonogenic and Transwell migration assays were performed, and the expression of epithelial-mesenchymal transition (EMT) proteins was detected to determine the metastatic functional roles of Nup62 in GC. Compared with the adjacent normal tissues, Nup62 was found to be upregulated in GC tissues using software prediction and detecting clinical specimens and cell lines. Moreover, the downregulation of Nup62 suppressed colony formation and decreased the number of migrated cells. In contrast, Nup62 overexpression promoted colony formation and increased the number of migrated cells. Further functional studies showed that the abnormal expression of Nup62 influenced cell migration and EMT through wingless/β-catenin (Wnt/β-catenin) and transforming growth factor (TGF)-β signaling pathways. In summary, the findings indicate that Nup62 regulates cell migration by interfering with Wnt/β-catenin and TGF-β signaling pathways in GC.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 2","pages":"81-87"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25565620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunohistochemical Expression of Emerin in Renal Cell Carcinomas: Does It Contribute to Histopathological Features and WHO/ISUP Grading in Predicting Prognosis? 肾细胞癌中Emerin的免疫组织化学表达:是否有助于组织病理学特征和WHO/ISUP分级预测预后?

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021037984

Asuman Argon, Gamze Dal, Tarik Salman

{"title":"Immunohistochemical Expression of Emerin in Renal Cell Carcinomas: Does It Contribute to Histopathological Features and WHO/ISUP Grading in Predicting Prognosis?","authors":"Asuman Argon, Gamze Dal, Tarik Salman","doi":"10.1615/JEnvironPatholToxicolOncol.2021037984","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021037984","url":null,"abstract":"Aim: In our study, a new grading model (e-GM) including nuclear membrane irregularity highlighted by emerin expression was proposed for renal cell carcinomas (RCC). It was aimed to investigate the relationship of this model with WHO/ISUP grading system, histopathological features, and prognosis.Methods and results: 86 RCC cases were included in the study. The mean age of the patients was 59.65, and the mean tumor size was 6.36 cm. According to pTNM staging, 45 of the cases were stage 1, 11 were stage 2, 26 were stage 3, and 4 were stage 4. According to e-GM grading, advanced tumor grade was found to be associated with perirenal tissue extension, necrosis, lymphovascular invasion, distant metastasis, advanced pT and TNM stage. Nuclear membrane irregularity caused an increase in tumor grade in 17 wi-GS grade 1 cases, 14 WHO/ISUP grading system (wi-GS) grade 2 cases, and 1 wi-GS grade 3 case. In the stepwise statistical analysis, it was determined that the most important prognostic factor was the TNM stage, followed by age and tumor size.Conclusions: Statistical analyses showed that nuclear membrane irregularity should be a criterion for classification according to e-GM in wi-grade 2 cases, but not necessarily in wi-grade 1 cases. Nuclear membrane irregularity was a prominent feature at high tumor grades, and its expression in RCCs suggests that it may be a target for tumor-specific treatments.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 2","pages":"89-98"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25565621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics Approach to the Exploration of Amino Acids Metabolism Changes Associated with Disease Progression in a Rat Model of Adjuvant-Induced Arthritis. 代谢组学方法探索佐剂诱导关节炎大鼠模型中与疾病进展相关的氨基酸代谢变化。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021039409

Yi Liu, Yunfei Xie

{"title":"Metabolomics Approach to the Exploration of Amino Acids Metabolism Changes Associated with Disease Progression in a Rat Model of Adjuvant-Induced Arthritis.","authors":"Yi Liu, Yunfei Xie","doi":"10.1615/JEnvironPatholToxicolOncol.2021039409","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021039409","url":null,"abstract":"The disease progression of rheumatoid arthritis (RA) is closely related to the disorder of amino acids metabolism. This study aimed to clarify the changes in the categories, quantities and metabolic pathways of amino acids associated with disease progression in adjuvant-induced arthritis (AIA) rats, and to evaluate the application value of amino acids metabolic profiling in the diagnosis of RA. A total of 20 rats were randomized into a control group and AIA model group. Thirty-three days after modeling, the synovial tissues of left ankle joints were collected for histopathological examination. Also, untargeted metabolomics based on UPLC-LTQ/Orbitrap MS was used to identify the potential amino acids and analyze the changes of their metabolic pathways. Histopathological observation showed that the synovial tissues of AIA rats exhibited hyperplastic and inflammatory lesions. Through multivariate statistical analysis, a total of 12 amino acids were identified and considered to show metabolic changes in AIA rats. Compared with the control group, the concentrations of glutamate, arginine, methionine were increased in the AIA model group; while the concentrations of proline, valine, tyrosine, phenylalanine, leucine, glycine, tryptophan, histidine, threonine were decreased. Metabolic pathway analysis showed that the selected amino acids had important physiological functions in immune and inflammatory response. The results suggested that amino acids could be employed as biomarkers closely related to RA, and the analysis of amino acids metabolic profiling also exhibited potential application value in the diagnosis, disease progression monitoring and therapy of RA.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 4","pages":"43-52"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39747693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Protective Role of Cirsilineol against Ovalbumin-Induced Allergic Rhinitis in Mice by Suppression of Inflammation and Oxidative Stress. 通过抑制炎症和氧化应激，鸡油菌素对卵清蛋白诱导的小鼠过敏性鼻炎具有保护作用

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021038489

Erle Li, Dong Wang, Yajuan Xue, Juan Yan, Juanli Wang

{"title":"The Protective Role of Cirsilineol against Ovalbumin-Induced Allergic Rhinitis in Mice by Suppression of Inflammation and Oxidative Stress.","authors":"Erle Li, Dong Wang, Yajuan Xue, Juan Yan, Juanli Wang","doi":"10.1615/JEnvironPatholToxicolOncol.2021038489","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2021038489","url":null,"abstract":"Allergic rhinitis (AR) is a common type of inflammatory disease with symptoms including rhinorrhea, fatigue, sneezing, and disturbed sleep. AR affects nearly 40% of peoples worldwide with the increased numbers of new cases. In this work, the study was conducted to disclose the anti-inflammatory and antiallergic properties of cirsilineol against the ovalbumin (OVA)-sensitized AR in mice. AR was provoked in BALB/c mice through the OVA challenge 30 days along with 10 and 20 mg/kg of cirsilineol treatment. The nasal symptoms, i.e., rubbing and sneezing was monitored after the final OVA challenge. The status of OVA-specific IgE, PGD2, and LTC4 was investigated using assay kits. The status of pro-inflammatory markers also examined using assay kits. The levels of oxidative markers, SOD activity, and pro-inflammatory markers in the spleen mononuclear cells (SMEs) were studied by using respective assay kits. The mRNA expression of TXNIP was assessed using RT-PCR study. The 10 and 20 mg/kg of cirsilineol treatment effectively decreased the sneezing and nasal rubbings in OVA-provoked mice. Cirsilineol also decreased the IgE, PGD2, and LTC4 status in the AR animals. The status of pro-inflammatory markers, i.e., IL-4, IL-5, IL-6, IL-33 and TNF-α was found to be decreased in the cirsilineol administered AR mice. Cirsilineol effectively reduced the ROS and MDA and improved SOD in the OVA-challenged SMCs. The mRNA expression of TXNIP was appreciably suppressed by the cirsilineol treatment. Altogether, these findings proved the beneficial actions of cirsilineol against the OVA-triggered AR in mice. The additional studies on the cirsilineol could lead to the development of new drug for AR management.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 3","pages":"63-73"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39468922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TUBB3 Promotes Growth and Invasion of Gallbladder Cancer Cells by Akt/mTOR Signal Pathway. TUBB3 通过 Akt/mTOR 信号通路促进胆囊癌细胞的生长和侵袭

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021036821

Zijun Liu, Suqin Li, Jianning Dong, Yi Miao

{"title":"TUBB3 Promotes Growth and Invasion of Gallbladder Cancer Cells by Akt/mTOR Signal Pathway.","authors":"Zijun Liu, Suqin Li, Jianning Dong, Yi Miao","doi":"10.1615/JEnvironPatholToxicolOncol.2021036821","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2021036821","url":null,"abstract":"Aberrant expression of β-tubulin-III (TUBB3) is known that related to aggressive tumor features and poor clinical outcomes. However, there is limited research about TUBB3 expression and its role in the outcomes and the progression of gallbladder cancer. We have measured TUBB3 level in gallbladder cancer samples and cell lines from 2012 to 2016, and tested the effects of TUBB3 in cancer cell growth, apoptosis and cell cycle arrest by using appropriate methods. The results revealed that TUBB3 was significantly over-expressed in gallbladder cancer samples and cell lines, and high TUBB3 level contributed to shorter overall survival in patients. The knockdown of TUBB3 with sh-TUBB3 inhibited the proliferation, migration and invasion of cancer cells. Meanwhile, it promotes apoptosis and changes the cell cycle distribution. Suppression of TUBB3 expression could increase p21 and cyclin B1 expression, and decrease cyclin D1. Xenograft mouse model also showed that low expression of TUBB3 reduced the growth of established gallbladder cancer xenograft in vivo. Furthermore, TUBB3 knockdown significantly decreased phosphorylation of Akt and mTOR in vitro and in vivo. TUBB3 can induce the development of gallbladder cancer by Akt/mTOR signal pathway and we point out a potential therapeutic target for gallbladder cancer treatment.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 2","pages":"23-33"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25564153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SET Domain-Containing Protein 5 Enhances the Cell Stemness of Non-Small Cell Lung Cancer via the PI3K/Akt/mTOR Pathway. SET结构域蛋白5通过PI3K/Akt/mTOR通路增强非小细胞肺癌的细胞干性

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021036991

Qing Chen, Zhuo Sun, Jinfang Li, Donghua Zhang, Bin Guo, Tongwen Zhang

{"title":"SET Domain-Containing Protein 5 Enhances the Cell Stemness of Non-Small Cell Lung Cancer via the PI3K/Akt/mTOR Pathway.","authors":"Qing Chen, Zhuo Sun, Jinfang Li, Donghua Zhang, Bin Guo, Tongwen Zhang","doi":"10.1615/JEnvironPatholToxicolOncol.2021036991","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021036991","url":null,"abstract":"Background: SET domain-containing protein 5 (SETD5) could promote non-small cell lung cancer (NS-CLC) cell invasion, but the effect of SETD5 on NSCLC cell stemness characteristics is unknown. Thus we attempted to evaluate the effect of SETD5 on NSCLC stemness and its mechanism.Methods: The expressions of SETD5 and stemness-related genes (SOX2, OCT4, ABCG2) were detected in NSCLC tissues by immunohistochemistry assay, qRT-PCR, and western blot. A SETD5 knockdown cell model was constructed by siRNA transfection in A549 and H1299 cells. A CCK8 assay was used to examine cell viability. A sphere-forming assay and side population cell assay were conducted to measure the cancer cell stem properties. The cells with SETD5 deletion were treated with an activator of AKT, SC79, and the protein expressions of Akt, p-Akt, mTOR, and p-mTOR were assessed.Results: SETD5 and cancer stem-related genes SOX2, OCT4, and ABCG2 were co-expressed and co-localized in tumor tissues and cell lines of NSCLC. The deletion of SETD5 significantly reduced the cell viability, cancer stem properties, and activity of the PI3K/Akt/mTOR pathway, while the decreased SETD5-induced effects were partially restored with SC79 treatment.Conclusion: In this study, SETD5 promoted the cancer stem cell property of NSCLC through mitigating the activation of the PI3K/Akt/mTOR pathway, suggesting a candidate target role for SETD5 in NSCLC treatment.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 2","pages":"55-63"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25564156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Betalain Alleviates Airway Inflammation in an Ovalbumin-Induced-Asthma Mouse Model via the TGF-β1/Smad Signaling Pathway. 甜菜碱通过 TGF-β1/Smad 信号通路缓解卵清蛋白诱导的哮喘小鼠模型的气道炎症

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021037050

Rui Dai, Yan Wang, Ningling Wang

{"title":"Betalain Alleviates Airway Inflammation in an Ovalbumin-Induced-Asthma Mouse Model via the TGF-β1/Smad Signaling Pathway.","authors":"Rui Dai, Yan Wang, Ningling Wang","doi":"10.1615/JEnvironPatholToxicolOncol.2021037050","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2021037050","url":null,"abstract":"Global industrialization not only improved the quality life of millions but also paved the way to solving many health problems. One among them is allergic asthma, which affects approximately 20% of the global population. Poor air quality is the major culprit in allergic asthma, which not only affects the individual's health, but also impairs his or her life quality and that of family members. Asthma is a chronic pulmonary inflammatory disease characterized by excess mucus production, airway hyperresponsiveness, and bronchoconstriction. Inhalation of corticosteroids, leukotriene modifiers, and β-adrenergic agonists is one treatment prescribed to control the symptoms of asthma, but there is still no effective cure. Phytochemicals such as carotenoids, phenolics, alkaloids, and nitrogen and organosulfur compounds are proven to possess immense pharmacological properties. Betalain is one such phytochemical present in plants of the order Caryophyllales. It is a water-soluble nitrogen-based pigment proven to possess antimicrobial, antioxidant, anti-inflammatory, hepatoprotective, antilipidemic, antidiabetic, and anticancer properties. We examined the curative potential of betalain against allergic asthma in a mouse model. Betalain treatment effectively decreased lung weight and infiltration of inflammatory cells in BAL fluid, and lowered IgE, eotaxin, and cytokine levels in asthma-induced mice. It also improved pulmonary mechanics and decreased oxidative stress and nitric oxide levels. Betalain significantly decreased gene expression of TGF-β and its downstream signaling Smad proteins. Lung histology confirmed that betalain protected the lung tissue of mice from ovalbumin-induced allergic asthma. Overall, our results show that betalain is a potent antiallergic drug that effectively protects mice from ovalbumin-induced allergic asthma. With further research, it can be prescribed as a treatment for asthma in humans.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 2","pages":"11-21"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25564152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxic Effects of Tetrabromobisphenol A: Focus on Endocrine Disruption. 四溴双酚A的毒性作用:重点是内分泌干扰。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021035595

Didem Oral, Aylin Balci, Ming-Wei Chao, Pinar Erkekoglu

{"title":"Toxic Effects of Tetrabromobisphenol A: Focus on Endocrine Disruption.","authors":"Didem Oral, Aylin Balci, Ming-Wei Chao, Pinar Erkekoglu","doi":"10.1615/JEnvironPatholToxicolOncol.2021035595","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021035595","url":null,"abstract":"Tetrabromobisphenol A (TBBPA) is a brominated flame retardant that is used in a variety of consumer products such as electronic equipment, fire extinguishers, furniture, plastics, textiles, and kitchen hoods. Most studies show that the TBBPA production process and TBBPA in industrial and urban sewage waste result in extensive human exposure and environmental contamination. TBBPA can accumulate in organisms, particularly aquatic life, and is classified as a group 2A carcinogen (likely carcinogenic to humans) by the International Agency for Research on Cancer. This compound produces low acute toxicity, but chronic exposure may produce serious consequences. In this review, we focus on TBBPA toxicity by discussing results of various studies that were published in the last two decades. Studies show that TBBPA acts as an endocrine disruptor, causing neurobehavioral and immunotoxic effects, oxidative stress, and apoptosis. Although several experiments were performed in vitro and in vivo, human data are lacking, and thus, chronic toxic effects of TBBPA on humans are not well known, particularly in sensitive populations including pregnant women, newborns, children, and the elderly. Epidemiological studies that comprehensively assess TBBPA levels in biological fluids of different populations and in different pathological conditions are needed. Research on the impact of TBBPA, particularly regarding endocrine disorders and cancer, must also be performed.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 3","pages":"1-23"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39468918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Anticancer Effect of Troxerutin in Human Non-Small-Cell Lung Cancer Cell A549 and Inhibition of Tumor Formation in BALB/c Nude Mice. 曲克芦丁对人类非小细胞肺癌细胞 A549 的抗癌作用以及对 BALB/c 裸鼠肿瘤形成的抑制作用

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021037951

Junlong Yu, Xiaohan Huang, Min Cao, Ling Qian, Lili Shao, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Jinni Jian

{"title":"Anticancer Effect of Troxerutin in Human Non-Small-Cell Lung Cancer Cell A549 and Inhibition of Tumor Formation in BALB/c Nude Mice.","authors":"Junlong Yu, Xiaohan Huang, Min Cao, Ling Qian, Lili Shao, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Jinni Jian","doi":"10.1615/JEnvironPatholToxicolOncol.2021037951","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2021037951","url":null,"abstract":"This study is intended to explore the anticancer, antiproliferative, and chemopreventive action of troxerutin (TX) in human non-small-cell lung cancer cell (A549) using BALB/c nude mice. 2 × 106 A549 cells were subcutaneously injected into mice, along with 10 μM and 20 μM/kg body weight of TX orally for 19 days. On the last day, tumor weight and volume were assessed. Stress marker enzymes such as Aryl hydrocarbon hydroxylase (AHH), lactate dehydrogenase (LDH), 5'Nucleotidase (5'ND), and γ-glutamyltranspeptidase (γ-GT) were estimated in the lung tissues. Cytotoxicity of TX was assessed using MTT assay. Expression of carcinoembryonic antigen (CEA) and inflammatory cytokines were also analyzed. Histopathological examination of tissue sections and immunohistochemical examination of proliferating cell nuclear antigen (PCNA) were also performed. mRNA expression of p53, p21, cyclin D1, P13k, Akt, and mTOR were analyzed using RT-PCR. TX administered orally in a dose-dependent manner markedly reverted the level of stress marker enzymes to a significant extent. TX also exhibited significant protection against lung cancer cells, as evidenced by cytotoxicity assay and histopathological studies. It was also found to reduce the expression of PCNA, cyclin D1, P13k, Akt, and mTOR, but increase the expression of p53 and p21. TX has also been shown to reduce cancer cell inflammation, as was evidenced by reduced expression of inflammatory cytokines. Thus TX could be used as an effective chemopreventive and anticancer agent in treating cancer.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 3","pages":"25-35"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39468919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0