Journal of Environmental Pathology Toxicology and Oncology最新文献_第8页

Tomentosin Reduces Behavior Deficits and Neuroinflammatory Response in MPTP-Induced Parkinson's Disease in Mice. 绒毛蛋白减少mptp诱导的帕金森病小鼠的行为缺陷和神经炎症反应。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.v40.i1.70

Yunhu Fan, M Maghimaa, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Sardar Hussain, Thamaraiselvan Rengarajan

{"title":"Tomentosin Reduces Behavior Deficits and Neuroinflammatory Response in MPTP-Induced Parkinson's Disease in Mice.","authors":"Yunhu Fan, M Maghimaa, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Sardar Hussain, Thamaraiselvan Rengarajan","doi":"10.1615/JEnvironPatholToxicolOncol.v40.i1.70","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.v40.i1.70","url":null,"abstract":"Parkinson's disease (PD) is the second most common neurodegenerative disease worldwide. Tomentosin is an active compound isolated from the I. viscosa plant that has extensive therapeutic value. In this exploration, the neuroprotective actions of tomentosin were investigated against MPTP-stimulated neuroinflammation in mice. PD was stimulated in C57/BL6 mice by injecting 20-mg/kg MPTP at 2-h intervals 4 times a day for 15 days simultaneously with tomentosin treatment. The rota-rod test, grasping test, and pole climbing test were executed to investigate the motor functioning of the test animals. Proinflammatory cytokines, reactive oxygen species, and myeloperoxidase were assayed using commercial ELISA kits. Superoxide dismutase enzyme levels were measured by the standard method. Expression of TLR-4/NF-κB was analyzed by Western blot. Brain tissues of investigational animals were analyzed microscopically. Tomentosin treatment of the MPTP-intoxicated PD mice promoted appreciable regains in body weight and noticeably prevented MPTP-stimulated impairments in motor function. In the PD mice, proinflammatory cytokine, ROS, and MPO levels were lowered by tomentosin, inhibited the TLR-4/NF-κB signaling pathway and prevented inflammation-mediated neuronal cell damage, and reduced glial cell damage and normalized ganglion layers. These findings confirmed the neuroprotective properties of tomentosin against MPTP-induced PD in mice.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 1","pages":"75-84"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25410263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

T3 Promotes Glioma Cell Senescence and Apoptosis via THRA and THRB. T3通过THRA和THRB促进胶质瘤细胞衰老和凋亡。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021038740

Xingkui Zhang, Wei Ma, Junjun Li, Chunyan Li, Wei Liu, Zhiwei Tang, Liyan Li

{"title":"T3 Promotes Glioma Cell Senescence and Apoptosis via THRA and THRB.","authors":"Xingkui Zhang, Wei Ma, Junjun Li, Chunyan Li, Wei Liu, Zhiwei Tang, Liyan Li","doi":"10.1615/JEnvironPatholToxicolOncol.2021038740","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021038740","url":null,"abstract":"Glioma is one of the most common types of primary intracranial tumors. The relationship between triiodothyronine (T3) and glioma is not clear. This study aimed to investigate the effect of T3 on the proliferation of glioma cells and its mechanism. Cell viability was analyzed by cell counting kit 8 assay. Flow cytometry analysis was used to detect cell apoptosis and cell cycle. Thyroid hormone receptor α (THRA) and thyroid hormone receptor β (THRB) were silenced by transfecting si-THRA and si-THRB plasmids into HS683 and A172 glioma cells. Western blot was performed to assess the protein expressions. The results indicated that triiodothyronine (T3) affected the viability, apoptosis and cell cycle of HS683 and A172 glioma cells. Cell apoptosis was significantly inhibited in si-THRA and si-THRB experimental groups. Moreover, knockdown of THRA and THRB reversed the G1 and G2 phase arrest led by T3 and induced an up-regulation of cyclin D1 expression. The phosphorylated extracellular signal-regulated kinase (p-ERK), p-AKT, and phosphorylated signal transducer and activator of transcription (p-STAT3) proteins were markedly increased by inhibiting THRA and THRB in HS683 and A172 glioma cells. T3 affected apoptosis and cell cycle of glioma cells through regulating THRA and THRB expressions. THRA and THRB may affect glioma development through regulating, at least partially, the mitogen-activated protein kinase (MAPK)/ERK and phosphoinositide 3-kinase (PI3K)/Akt signaling pathways.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 4","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39747288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Propofol Ameliorates the Proliferation and Epithelial-Mesenchymal Transition of Hepatoma Carcinoma Cells via Non-Coding RNA Activated by DNA Damage (NORAD)/microRNA (miR)-556-3p/Migration and Invasion Enhancer 1 (MIEN1) Axis. 异丙酚通过DNA损伤激活的非编码RNA (NORAD)/microRNA (miR)-556-3p/迁移和侵袭增强子1 (MIEN1)轴促进肝癌细胞增殖和上皮间质转化

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021039471

Yanna Liu, Xiang Wang, Hongyu Li, Enjian Guan, Kaidi Luo

{"title":"Propofol Ameliorates the Proliferation and Epithelial-Mesenchymal Transition of Hepatoma Carcinoma Cells via Non-Coding RNA Activated by DNA Damage (NORAD)/microRNA (miR)-556-3p/Migration and Invasion Enhancer 1 (MIEN1) Axis.","authors":"Yanna Liu, Xiang Wang, Hongyu Li, Enjian Guan, Kaidi Luo","doi":"10.1615/JEnvironPatholToxicolOncol.2021039471","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021039471","url":null,"abstract":"Propofol has been previously demonstrated to relieve hepatocellular carcinoma (HCC). However, the specific molecular mechanisms mediated by propofol remain to be explored. mRNA or miRNA expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Protein expression was determined by Western blot. The interaction between microRNA (miR)-556-3p and long coding RNA non-coding RNA activated by DNA damage (NORAD) or migration and invasion enhancer 1 (MIEN1) was verified by luciferase reporter gene and RNA pull-down assays. Cellular functions were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetra-zolium bromide (MTT), 5-ethynyl-2'-deoxyuridine (EdU), and Transwell assays. Propofol notably suppressed the proliferation and EMT of Hep3B and SNU449 cell lines. NORAD was overexpressed in the HCC tissues and cells, while propofol decreased NORAD levels in the HCC cells. Conversely, overexpression of NORAD partially restored malignant behaviors of the HCC cells and abolished the effects of propofol. Additionally, NORAD sponged miR-556-3p to upregulate MIEN1. However, the knockdown of MIEN1 suppressed the proliferation and EMT of HCC cells. Propofol inhibited HCC cell proliferation and EMT progress via NORAD/miR-556-3p/MIEN1 axis. These data provided a potent prognosis and diagnostic marker for HCC and supplemented the underlying mechanism of propofol-induced anti-tumor effects.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 4","pages":"87-97"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39747697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Neferine Inhibits 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumorigenesis by Suppression of Cell Proliferation and Induction of Apoptosis via Modulation of the PI3K/AKT/NF-κB Signaling Pathway. 通过调节PI3K/AKT/NF-κB信号通路，莲子碱抑制7,12-二甲基苯(a)蒽诱导的乳腺肿瘤发生，抑制细胞增殖和诱导凋亡。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021038118

Linhan Yang, Ying Xiong, Zhongli Sun, Xiaoying Lin, Huaikun Ni

{"title":"Neferine Inhibits 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumorigenesis by Suppression of Cell Proliferation and Induction of Apoptosis via Modulation of the PI3K/AKT/NF-κB Signaling Pathway.","authors":"Linhan Yang, Ying Xiong, Zhongli Sun, Xiaoying Lin, Huaikun Ni","doi":"10.1615/JEnvironPatholToxicolOncol.2021038118","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021038118","url":null,"abstract":"Aim: To investigate the anticancer mechanism of neferine on DMBA-prompted mammary tumorigenesis in animals.Methods: Mammary cancer was prompted by the subcutaneous injection of 25 mg DMBA mixed in 1 ml of the vehicle (sunflower oil [0.5 ml] and saline [0.5 ml]). We analyzed the biochemical and molecular expression of cell-proliferation and apoptotic markers in normal and DMBA-induced rats.Results: We detected low body weight, elevated quantities of lipid peroxidation, and low antioxidant enzyme activities in mammary tissues of DMBA-induced animals. We also found an invasive ductal carcinoma in DMBA-induced animals by histopathological assessment. Furthermore, western blotting findings displayed an augmented expression of PI3K, AKT, NF-κB, PCNA, cyclin D1, Ki-67, and Bcl-2, while reducing expression of p53, Bax, caspase-3, and caspase-9 in DMBA-induced cancer-bearing animals. RT-PCR results found upregulation of cyclin D1, PCNA, and Ki-67, and reduced expression of p53 in DMBA-prompted animals. The oral administration of neferine effectually inhibited mammary tumors via improved antioxidants and prevented lipid peroxidation activities when compared with tumor-bearing rats. Furthermore, neferine also modulated PI3K/AKT/NF-κB signaling through inhibiting cell proliferation and induced apoptosis in tumor-bearing rats.Conclusion: In our findings, we concluded that neferine has an anti-proliferative and enhancing apoptotic property against DMBA-induced mammary cancer.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 3","pages":"51-61"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39468921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Protective Effect of Isopulegol in Alleviating Neuroinflammation in Lipopolysaccharide-Induced BV-2 Cells and in Parkinson Disease Model Induced with MPTP. 异普乐醇对缓解脂多糖诱导的 BV-2 细胞神经炎症和 MPTP 诱导的帕金森病模型的保护作用

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021038944

Bei Kang, Nini Li, Sha Liu, Shipei Qi, Shengzhi Mu

{"title":"Protective Effect of Isopulegol in Alleviating Neuroinflammation in Lipopolysaccharide-Induced BV-2 Cells and in Parkinson Disease Model Induced with MPTP.","authors":"Bei Kang, Nini Li, Sha Liu, Shipei Qi, Shengzhi Mu","doi":"10.1615/JEnvironPatholToxicolOncol.2021038944","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2021038944","url":null,"abstract":"Background: Parkinson's disease (PD) is the most prevalent disease linked with age-associated neuronal degeneration. Phytotherapeutic compounds or agents have gained increased importance because of their increased specificity and minimal side effects. Isopulegol, a monoterpene, was utilized in the present study because of its wide range of therapeutic properties. Our aim was to examine the underlying mechanism of anti-neuroinflammatory action and neuroprotective efficacy of isopulegol in cell lines and in an experimental animal model of PD.Methods: The MTT assay was performed in microglial BV-2 cells subjected to lipopolysaccharides (LPS). The release of NO and synthesis of ROS intracellularly in BV-2 cells were detected. C57BL/6 mice induced with MPTP were examined for motor function and coordination. Expression of proinflammatory mediators was also assessed both in vivo and in vitro. Histopathological sections of brain and expression of iNOS and COX-2 were also analyzed.Results: BV-2 cells did not exhibit noticeable toxicity at selected concentrations and LPS-incubated cells showed marked elevation of NO levels and increased production of intracellular ROS. Increased expression of proinflammatory cytokines was also observed. Motor function and coordination deficits were observed in mice induced with MPTP. Histopathological abnormalities and increased iNOS and COX-2 expression were noted in MPTP-induced mice. Administration of isopulegol reversed the changes brought about by LPS and MPTP.Conclusion: The study indicated that isopulegol is a potential therapeutic drug against clinical complications of PD.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 3","pages":"75-85"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39468923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Expression of Annexin A9 Promotes Cell Proliferation and Migration in Gastric Cancer via the TGF-β Signaling Pathway. 膜联蛋白A9高表达通过TGF-β信号通路促进胃癌细胞增殖和迁移

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021038527

Yong Zhou, Caiyu Qiu, Tao Wang, Lisheng Tao, Zhaoguo Zhang, Jun Yao

{"title":"High Expression of Annexin A9 Promotes Cell Proliferation and Migration in Gastric Cancer via the TGF-β Signaling Pathway.","authors":"Yong Zhou, Caiyu Qiu, Tao Wang, Lisheng Tao, Zhaoguo Zhang, Jun Yao","doi":"10.1615/JEnvironPatholToxicolOncol.2021038527","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021038527","url":null,"abstract":"Annexin A9 (ANXA9) represents an important calcium-dependent phospholipid-binding protein family member and contains a calcium-binding site that is necessary for extracellular matrix proteins. ANXA9 has a significant role in human cancers. However, there is no correlation study existing on ANXA9 in gastric cancer (GC). ANXA9 messenger RNA (mRNA) expression within patients with GC were detected with reverse transcription polymerase chain reaction and its protein expression in GC and GES-1 cells were detected through Western blotting. ANXA9 levels within normal and GC tissue samples were measured by Kaplan-Meier analysis and Oncomine. Transwell migration, colony formation, and cell cycle assay monitored the effects of ANXA9 on cell proliferation and metastasis and growth. Additionally, proteins related to epithelial-mesenchymal transition (EMT) were detected to evaluate the function of ANXA9 within GC cells. Relative to GES-1 cells, ANXA9 expression increased within GC cells. Also, ANXA9 expression increased in GC tissues and indicated an unfavorable prognosis. Furthermore, ANXA9 over-expression within HGC-27 cells increased migrated cells quantity and formed larger and more numerous cell clones; the G1 phase decreased while S and G2 phases increased; whereas ANXA9 knockdown suppressed MGC-803 cell growth and migration. Thus, ANXA9 may influence cell growth, migration and EMT through transforming growth factor β (TGF-β) signal transduction pathway. Immunofluorescence analyzed SMAD2/3 and p-SMAD2/3 distribution and expression when ANXA9 was overexpressed in HGC-27 cells. These results predicted that ANXA9 mediated cell migration and growth through TGF-β signal transduction pathway within GC.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 3","pages":"87-94"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39468924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Differential Analysis of IncRNAs and mRNAs Expression in HCC and the Predictive Value of lncRNAs. HCC中incrna和mrna表达差异分析及lncrna的预测价值

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2021039724

Lei Shi, Qi Wang, Jie Lv, Qin Liu, Jiao Wu

{"title":"Differential Analysis of IncRNAs and mRNAs Expression in HCC and the Predictive Value of lncRNAs.","authors":"Lei Shi, Qi Wang, Jie Lv, Qin Liu, Jiao Wu","doi":"10.1615/JEnvironPatholToxicolOncol.2021039724","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021039724","url":null,"abstract":"Background: Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death. However, the molecular mechanism of its pathogenesis is still unclear. This study analyzed the pathophysiology of HCC based on the perspective of lncRNAs and mRNAs expression to find effective diagnostic strategies and prognostic markers.Methods: Data for HCC patients and normal controls were downloaded from the GEO and TCGA databases. LncRNAs and mRNAs differentially expressed in HCC patients were screened by R language (3.6.0). The ROC curves of the ten lncRNAs with the most significant expression differences were drawn, and survival analysis was conducted. Then, the differentially expressed mRNAs were annotated by using GO and KEGG databases. The protein-protein interactions in the lncRNA-mRNA coexpression network were analyzed with the STRING database. Tissue and cell experiments were performed to verify the results of bioinformatics analysis.Results: A large number of differentially expressed lncRNAs and mRNAs in HCC were correlated. Four lncRNAs were significantly associated with the prognosis of HCC patients. These differentially expressed lncRNAs mainly regulated the proliferation and lipid metabolism of HCC. Cell experiments confirmed that lnc-MMADHC-5 could inhibit the development of HCC.Conclusion: lncRNAs regulated the proliferation and lipid metabolism of HCC. Lnc-APBB1-1, lnc-FBXO42-1, lnc-JAKMIP2-1, and lnc-MMADHC-5 might be prognostic markers for HCC. Lnc-MMADHC-5 could inhibit the development of HCC.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 4","pages":"73-85"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39747696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Coronavirus Disease 2019 (COVID-19): Research, Clinical Knowledge, and Preventive Measures. 2019冠状病毒病(COVID-19):研究、临床知识和预防措施

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2020036207

Richa Rathor, Geetha Suryakumar, Som Nath Singh, Bhuvnesh Kumar

{"title":"Coronavirus Disease 2019 (COVID-19): Research, Clinical Knowledge, and Preventive Measures.","authors":"Richa Rathor, Geetha Suryakumar, Som Nath Singh, Bhuvnesh Kumar","doi":"10.1615/JEnvironPatholToxicolOncol.2020036207","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2020036207","url":null,"abstract":"In early December 2019, a novel coronavirus disease 2019 (COVID-19), the global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) commenced in Wuhan, China, and WHO declared the outbreak a pandemic and Public Health Emergency of International Concern. An ample number of clinical trials with multiple drugs is underway to overcome the current perilous condition. Still, the situation is alarming with no therapeutic measure in our hand at present. Keeping the present scenario in mind, this review comprises the research, clinical knowledge, and repurposed herbals with regard to COVID-19. Preventive measures such as yoga, nasal breathing, and herbal administration could also provide protection and beneficial effects against coronavirus. Innumerable clinical trials are ongoing to manage COVID-19 and the drugs were selected on the basis of life cycle of coronavirus. The selection of herbals was done on the basis of the previous reported pharmacological activities and docking study. The results concluded that garlic, liquorice, and Ashwagandha have a potential against SARS-CoV-2, which was further proved via a docking study and their reported biological functions. The very well-known fact \"prevention is always better than cure\" is applied to overcome with coronavirus infection. It is expected that following the preventive measures could impede or lessen the adverse effect of SARS-CoV-2.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 1","pages":"29-42"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25409779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Perls' Prussian Blue Stains of Lung Tissue, Bronchoalveolar Lavage, and Sputum. 肺组织、支气管肺泡灌洗液和痰液的珀尔斯普鲁士蓝染色。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2020036292

Andrew J Ghio, Victor L Roggli

{"title":"Perls' Prussian Blue Stains of Lung Tissue, Bronchoalveolar Lavage, and Sputum.","authors":"Andrew J Ghio, Victor L Roggli","doi":"10.1615/JEnvironPatholToxicolOncol.2020036292","DOIUrl":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2020036292","url":null,"abstract":"Perls' Prussian blue (PPB) stain recognizes Fe3+ associated with hemosiderin. The employment of this stain in clinical medicine and research has been extensive and novel applications continue to evolve. Ferruginous bodies are intracellular structures in lung tissue, bronchoalveolar lavage (BAL), and sputum that stain with PPB. Inhaled, insoluble, biopersistent particles and fibers are phagocytosed by lung macrophages and thought to be coated, either partially or completely, with an iron-containing protein at the interface forming a ferruginous body. These structures can be categorized as ferruginous bodies having either an inorganic or a carbonaceous core (e.g., asbestos and byssinotic bodies, respectively). In lung tissue, BAL, and sputum, the only cells that stain with PPB are macrophages. These are described as iron- and hemosiderin-laden macrophages and called either siderophages or sideromacrophages. Siderophages can be observed in the lung tissue, BAL, and sputum after various exposures and can also be associated with many different pulmonary and extrapulmonary diseases.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 1","pages":"1-15"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8981511/pdf/nihms-1686444.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25409780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Whole Exome Sequencing Identifies Cohesin Component STAG1 Mutation in de novo Acute Myeloid Leukemia (FAB M2): A Pilot Study with Cytogenetics, Clinical and Prognostic Implications. 全外显子组测序发现新发急性髓性白血病（FAB M2）中的粘合素成分 STAG1 基因突变：一项具有细胞遗传学、临床和预后意义的试点研究。

IF 2.4 4区医学

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2021-01-01 DOI: 10.1615/JEnvironPatholToxicolOncol.2020035890

Kunnathur Murugesan Sakthivel, Narayanan Geetha, Thampirajan Vimaladevi Akhila Raj, Ramachandran Krishna Chandran, Kumarapillai Mohanan Nair Jagathnath Krishna, Hariharan Sreedharan

{"title":"Whole Exome Sequencing Identifies Cohesin Component STAG1 Mutation in de novo Acute Myeloid Leukemia (FAB M2): A Pilot Study with Cytogenetics, Clinical and Prognostic Implications.","authors":"Kunnathur Murugesan Sakthivel, Narayanan Geetha, Thampirajan Vimaladevi Akhila Raj, Ramachandran Krishna Chandran, Kumarapillai Mohanan Nair Jagathnath Krishna, Hariharan Sreedharan","doi":"10.1615/JEnvironPatholToxicolOncol.2020035890","DOIUrl":"10.1615/JEnvironPatholToxicolOncol.2020035890","url":null,"abstract":"The clinical implications of cohesin gene complex mutation in acute myeloid leukemia (AML) are not well characterized. In the present study, a cohort of 152 de novo unselected adult AML patients underwent conventional and molecular cytogenetic analysis for chromosomal aberrations. Further, we examined the frequency and clinical implications of mutations in cohesin gene complex STAG1, STAG2, RAD21, SMC1, and SMC3 using whole exome sequencing as a pilot study in 10 de novo patients with AML-FAB M2. Among the 10 cases, we identified a functionally heterozygous mutation in exon16 of STAG1 in one patient (10%), however no mutation was observed in STAG2, RAD21, SMC1, and SMC3. Sanger sequencing analysis for exon 16 of STAG1 in the remaining 142 AML cases did not reveal any further mutations, which underlined the observation that mutations took place throughout the cohesin gene complex without presence of a mutational hot spot region. The present study identified a positive correlation between serum bilirubin, LDH, and hematological parameters such as Hb, WBC, and platelet count with STAG1 mutation. Our data suggest that the cohesin complex may represent an attractive therapeutic target for future preclinical and clinical studies. However, more studies with a larger number of patients should be performed prospectively to determine the pathogenic involvement of STAG 1 mutation in AML patients.","PeriodicalId":50201,"journal":{"name":"Journal of Environmental Pathology Toxicology and Oncology","volume":"40 1","pages":"51-64"},"PeriodicalIF":2.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25410261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2