npj Biosensing最新文献_第2页

Organic electrolyte gated field effect transistor for detecting alpha synuclein longitudinally in Parkinsonism mouse model. 有机电解质门控场效应晶体管在帕金森小鼠模型中纵向检测α -突触核蛋白。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-11-07 DOI: 10.1038/s44328-025-00057-8

Srishti Johri, Dennis Chan, Roslyn S Massey, Daniel Knight, Maria C DeRosa, Matthew R Holahan, Ravi Prakash

{"title":"Organic electrolyte gated field effect transistor for detecting alpha synuclein longitudinally in Parkinsonism mouse model.","authors":"Srishti Johri, Dennis Chan, Roslyn S Massey, Daniel Knight, Maria C DeRosa, Matthew R Holahan, Ravi Prakash","doi":"10.1038/s44328-025-00057-8","DOIUrl":"10.1038/s44328-025-00057-8","url":null,"abstract":"Parkinson's Disease (PD) is an age-progressive disorder caused by misfolding of alpha-synuclein (α-Syn) that can begin years before clinical symptoms appear, making early diagnosis crucial for timely intervention. In this study, a novel antibody-functionalized Organic Electrolyte-Gated Field-Effect-Transistor (Ab-OEGFET) biosensor was implemented to detect α-Syn levels in blood serum samples from an A53T Transgenic (TG) mouse line. PD-like pathology was examined in blood serum using Ab-OEGFET devices and in brain tissue samples using Western Blot and immunohistochemistry. Different forms (monomeric, phosphorylated, oligomeric) of α-Syn were identified in low volumes of blood serum samples collected from TG and Wild Type (WT) populations of mice at ages 2, 5 and 8 months, and the biosensor response was correlated to Blot and immunohistochemistry results. The Ab-OEGFETs performance in this study is a promising result towards a minimally invasive blood biomarker-based multianalyte testing strategy for early screening of PD and similar neurodegenerative disease pathologies.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12594612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145484445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmonic biosensors and actuators for integrated point-of-care diagnostics. 用于综合护理点诊断的等离子体生物传感器和执行器。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-12-08 DOI: 10.1038/s44328-025-00050-1

Han Truong, Trung Hoang, Kyungwha Chung, Luke P Lee

引用次数: 0

A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens. 同日α-突触核蛋白RT-QuIC种子扩增法检测突触核蛋白病生物标本。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-02-11 DOI: 10.1038/s44328-024-00023-w

Sabiha Parveen, Parvez Alam, Christina D Orrù, Sarah Vascellari, Andrew G Hughson, Wen-Quan Zou, Thomas G Beach, Geidy E Serrano, David S Goldstein, Bernardino Ghetti, Giovanni Cossu, Giada Pisano, Beatrice Pinna, Byron Caughey

{"title":"A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens.","authors":"Sabiha Parveen, Parvez Alam, Christina D Orrù, Sarah Vascellari, Andrew G Hughson, Wen-Quan Zou, Thomas G Beach, Geidy E Serrano, David S Goldstein, Bernardino Ghetti, Giovanni Cossu, Giada Pisano, Beatrice Pinna, Byron Caughey","doi":"10.1038/s44328-024-00023-w","DOIUrl":"10.1038/s44328-024-00023-w","url":null,"abstract":"Parkinson's disease (PD), dementia with Lewy bodies (DLB), and other synucleinopathies are characterized by the accumulation of abnormal, self-propagating aggregates of α-synuclein. RT-QuIC or seed amplification assays are currently showing unprecedented diagnostic sensitivities and specificities for synucleinopathies even in prodromal phases years in advance of the onset of Parkinsonian signs or dementia. However, commonly used α-synuclein seed amplification assays take ≥48 h to perform as applied to patients' diagnostic biospecimens. Here, we report the development of a faster α-synuclein RT-QuIC assay that is as analytically sensitive as prior assays of this type, but can be completed in ≤12 h for brain, skin, and intestinal mucosa, with positive signals often arising in <5 h. CSF assays took a few hours longer. Our same-day α-synuclein RT-QuIC (sdRT-QuIC) assay should increase the practicality, cost-effectiveness, and throughput of measurements of pathological forms of α-synuclein for fundamental research, clinical diagnosis, and therapeutics development.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic nano-plasmonic imaging platform for purification- and label-free single small extracellular vesicle characterization. 微流控纳米等离子体成像平台，用于纯化和无标记的单个小细胞外囊泡表征。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-07-30 DOI: 10.1038/s44328-025-00047-w

Omid Mohsen Daraei, Avinash Kumar Singh, Saswat Mohapatra, Mohammad Sadman Mallick, Abhay Kotnala, Wei-Chuan Shih

{"title":"Microfluidic nano-plasmonic imaging platform for purification- and label-free single small extracellular vesicle characterization.","authors":"Omid Mohsen Daraei, Avinash Kumar Singh, Saswat Mohapatra, Mohammad Sadman Mallick, Abhay Kotnala, Wei-Chuan Shih","doi":"10.1038/s44328-025-00047-w","DOIUrl":"10.1038/s44328-025-00047-w","url":null,"abstract":"Tumor-derived circulating small extracellular vesicles (sEVs) are a promising class of non-invasive biomarkers for disease diagnosis. However, their quantitative detection remains challenging due to their small size and the complexity of blood plasma. Therefore, sample preparation, such as purification and fluorescence labeling, is required. This study presents a purification-free approach using a microfluidic chip integrated with PlAsmonic NanO-apeRture lAbel-free iMAging (PANORAMA) for label-free single sEV characterization in plasma. CD63, CD9, and CD81 antibodies, specific for most sEVs surface antigens, are functionalized on arrayed gold nanodisks on invisible substrates (AGNIS) for selective capture. The automated microfluidic platform minimizes operational errors and biases and enables precise control of flow rates, directions, media volume, and composition for optimization. This platform requires only 20 µL of plasma, and the analysis is completed within 60 minutes. This platform shows great potential as a sensitive and effective tool for detecting and characterizing circulating sEVs without purification or labeling.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"26"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12310528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of electrical impedance spectroscopy of bovine eyes for early detection of uveal melanoma 牛眼电阻抗谱对葡萄膜黑色素瘤早期检测的评价

npj Biosensing Pub Date : 2024-12-27 DOI: 10.1038/s44328-024-00022-x

Vaibhav B. Yadav, Enosh Lim, Alison H. Skalet, Mohammad J. Moghimi

引用次数: 0

Fully automated sample to result SIMPLE RPA microfluidic chip towards in ovo sexing application 全自动化采样结果SIMPLE RPA微流控芯片在蛋类检测中的应用

npj Biosensing Pub Date : 2024-12-26 DOI: 10.1038/s44328-024-00019-6

Simão Monteiro Belo dos Santos, Celine Wegsteen, Dries Vloemans, Matthias Corion, Bart De Ketelaere, Dragana Spasic, Jeroen Lammertyn

{"title":"Fully automated sample to result SIMPLE RPA microfluidic chip towards in ovo sexing application","authors":"Simão Monteiro Belo dos Santos, Celine Wegsteen, Dries Vloemans, Matthias Corion, Bart De Ketelaere, Dragana Spasic, Jeroen Lammertyn","doi":"10.1038/s44328-024-00019-6","DOIUrl":"10.1038/s44328-024-00019-6","url":null,"abstract":"Several European countries have implemented legislations to eliminate day-old male chicks killing. Although embryo sexing (in ovo sexing) is the most promising alternative, no current solution can handle all egg colors with >98% sexing accuracy, low cost and minimal embryo disturbance before day 13 of incubation and processing >20,000 eggs/hour. Recombinase polymerase amplification (RPA) presents a promising alternative to PCR that can be integrated into microfluidic platforms. In this work, we developed fully autonomous microfluidic cartridge (SIMPLE-RPA chip) for female chick-specific synthetic HINTW gene detection in 30 min at 37.7 °C inside an egg incubator. We first optimized off-chip RPA, allowing for highly sensitive DNA detection (1.6 × 10–5 ng/µL). The SIMPLE-RPA chip was developed to automate the RPA bioassay on-chip, reducing user errors, and contamination risks and maintaining the off-chip LOD while offering low price, small footprint, upscaling compatibility, and easy transfer to other point-of-care applications.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00019-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements of paper-based sensors for antibiotic-resistant bacterial species identification 纸张传感器在耐药细菌种类鉴定中的应用进展

npj Biosensing Pub Date : 2024-12-13 DOI: 10.1038/s44328-024-00016-9

Aayushi Laliwala, Ashruti Pant, Denis Svechkarev, Marat R. Sadykov, Aaron M. Mohs

引用次数: 0

A quantitative, label-free visual interference colour assay platform for protein targeting and binding assays 一个定量的，无标签的视觉干扰颜色分析平台，用于蛋白质靶向和结合分析

npj Biosensing Pub Date : 2024-12-05 DOI: 10.1038/s44328-024-00017-8

Ana Lopez-Campistrous, Hillary Sweet, Ciaran Terry, Craig Garen, Yu Wan, Robert E. Burrell, Kyle Moxham, Matthew Nickel, Michael Serpe, Michael Joyce, Lorne Tyrrell, Todd P. W. McMullen

{"title":"A quantitative, label-free visual interference colour assay platform for protein targeting and binding assays","authors":"Ana Lopez-Campistrous, Hillary Sweet, Ciaran Terry, Craig Garen, Yu Wan, Robert E. Burrell, Kyle Moxham, Matthew Nickel, Michael Serpe, Michael Joyce, Lorne Tyrrell, Todd P. W. McMullen","doi":"10.1038/s44328-024-00017-8","DOIUrl":"10.1038/s44328-024-00017-8","url":null,"abstract":"The vast array of immunoassay technologies used to assess protein interactions is costly or platform-specific. We present a label-free visual interference colour assay (VICA) that quantifies peptide and protein interactions by creating an iridescent surface allowing direct visualisation without spectrophotometric optics or microfluidics. A nanoporous aluminium oxide surface is tuned to match the refractive indices of the overlying protein layers to generate visual interference colours. To functionalise the surface, we created an affinity-capture system using a protein A-carboxyglutamic (GLA) construct that orients antibodies to enhance the signal. Using off-the-shelf antibodies, the platform can isolate analytes in buffer, whole blood, or serum. This surface generates a discernible colour change at concentrations as low as 50 femtomoles/mm2 and can monitor oligomer formation in sequential steps on the same slide. VICA provides comparable kinetic parameters to biolayer interferometry and traditional immunoassays while also allowing characterisation of proteins in large macromolecular complexes.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00017-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photovoltaic bioelectronics merging biology with new generation semiconductors and light in biophotovoltaics photobiomodulation and biosensing 光电生物电子学将生物学与新一代半导体和生物光电学中的光融合在一起光生物调制和生物传感

npj Biosensing Pub Date : 2024-11-21 DOI: 10.1038/s44328-024-00015-w

Ebin Joseph, Manuela Ciocca, Haodong Wu, Serena Marcozzi, Maria Assunta Ucci, Kavya Keremane, Luyao Zheng, Bed Poudel, Congcong Wu, Antonella Camaioni, Kai Wang, Shashank Priya, Thomas M. Brown

引用次数: 0

Energy landscape of conformational changes for a single unmodified protein 单一未修饰蛋白质构象变化的能量图谱

npj Biosensing Pub Date : 2024-11-06 DOI: 10.1038/s44328-024-00014-x

Matthew Peters, Tianyu Zhao, Sherin George, Viet Giang Truong, Síle Nic Chormaic, Cuifeng Ying, René A. Nome, Reuven Gordon

引用次数: 0