npj Biosensing最新文献

Advancements of paper-based sensors for antibiotic-resistant bacterial species identification

npj Biosensing Pub Date : 2024-12-13 DOI: 10.1038/s44328-024-00016-9

Aayushi Laliwala, Ashruti Pant, Denis Svechkarev, Marat R. Sadykov, Aaron M. Mohs

引用次数: 0

A quantitative, label-free visual interference colour assay platform for protein targeting and binding assays

npj Biosensing Pub Date : 2024-12-05 DOI: 10.1038/s44328-024-00017-8

Ana Lopez-Campistrous, Hillary Sweet, Ciaran Terry, Craig Garen, Yu Wan, Robert E. Burrell, Kyle Moxham, Matthew Nickel, Michael Serpe, Michael Joyce, Lorne Tyrrell, Todd P. W. McMullen

{"title":"A quantitative, label-free visual interference colour assay platform for protein targeting and binding assays","authors":"Ana Lopez-Campistrous, Hillary Sweet, Ciaran Terry, Craig Garen, Yu Wan, Robert E. Burrell, Kyle Moxham, Matthew Nickel, Michael Serpe, Michael Joyce, Lorne Tyrrell, Todd P. W. McMullen","doi":"10.1038/s44328-024-00017-8","DOIUrl":"10.1038/s44328-024-00017-8","url":null,"abstract":"The vast array of immunoassay technologies used to assess protein interactions is costly or platform-specific. We present a label-free visual interference colour assay (VICA) that quantifies peptide and protein interactions by creating an iridescent surface allowing direct visualisation without spectrophotometric optics or microfluidics. A nanoporous aluminium oxide surface is tuned to match the refractive indices of the overlying protein layers to generate visual interference colours. To functionalise the surface, we created an affinity-capture system using a protein A-carboxyglutamic (GLA) construct that orients antibodies to enhance the signal. Using off-the-shelf antibodies, the platform can isolate analytes in buffer, whole blood, or serum. This surface generates a discernible colour change at concentrations as low as 50 femtomoles/mm2 and can monitor oligomer formation in sequential steps on the same slide. VICA provides comparable kinetic parameters to biolayer interferometry and traditional immunoassays while also allowing characterisation of proteins in large macromolecular complexes.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00017-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photovoltaic bioelectronics merging biology with new generation semiconductors and light in biophotovoltaics photobiomodulation and biosensing 光电生物电子学将生物学与新一代半导体和生物光电学中的光融合在一起光生物调制和生物传感

npj Biosensing Pub Date : 2024-11-21 DOI: 10.1038/s44328-024-00015-w

Ebin Joseph, Manuela Ciocca, Haodong Wu, Serena Marcozzi, Maria Assunta Ucci, Kavya Keremane, Luyao Zheng, Bed Poudel, Congcong Wu, Antonella Camaioni, Kai Wang, Shashank Priya, Thomas M. Brown

引用次数: 0

Energy landscape of conformational changes for a single unmodified protein 单一未修饰蛋白质构象变化的能量图谱

npj Biosensing Pub Date : 2024-11-06 DOI: 10.1038/s44328-024-00014-x

Matthew Peters, Tianyu Zhao, Sherin George, Viet Giang Truong, Síle Nic Chormaic, Cuifeng Ying, René A. Nome, Reuven Gordon

引用次数: 0

Recent advances in solid-liquid triboelectric nanogenerators for self-powered chemical and biological sensing 用于自供电化学和生物传感的固液三电纳米发电机的最新进展

npj Biosensing Pub Date : 2024-10-04 DOI: 10.1038/s44328-024-00011-0

Kuldeep Kaswan, Meenakshi Ray, Arshad Khan, Yu-Lin Wang, Zong-Hong Lin

引用次数: 0

EEG-based headset sleep wearable devices 基于脑电图的头戴式睡眠可穿戴设备

npj Biosensing Pub Date : 2024-10-01 DOI: 10.1038/s44328-024-00013-y

Karmen Markov, Mohamed Elgendi, Carlo Menon

引用次数: 0

Label-free functional imaging of vagus nerve stimulation-evoked potentials at the cortical surface 皮层表面迷走神经刺激诱发电位的无标记功能成像

npj Biosensing Pub Date : 2024-09-10 DOI: 10.1038/s44328-024-00012-z

Laura RoaFiore, Trevor Meyer, Thaissa Peixoto, Pedro Irazoqui

引用次数: 0

Towards early diagnosis and screening of Alzheimer’s disease using frequency locked whispering gallery mode microtoroids 利用频率锁定的耳语廊模式微透镜实现阿尔茨海默病的早期诊断和筛查

npj Biosensing Pub Date : 2024-08-28 DOI: 10.1038/s44328-024-00009-8

Adley Gin, Phuong-Diem Nguyen, Geidy Serrano, Gene E. Alexander, Judith Su

引用次数: 0

Realtime bacteria detection and analysis in sterile liquid products using deep learning holographic imaging 利用深度学习全息成像技术实时检测和分析无菌液体产品中的细菌

npj Biosensing Pub Date : 2024-08-23 DOI: 10.1038/s44328-024-00008-9

Nicholas Bravo-Frank, Rushikesh Zende, Lei Feng, Nicolas Mesyngier, Aditya Pachpute, Jiarong Hong

{"title":"Realtime bacteria detection and analysis in sterile liquid products using deep learning holographic imaging","authors":"Nicholas Bravo-Frank, Rushikesh Zende, Lei Feng, Nicolas Mesyngier, Aditya Pachpute, Jiarong Hong","doi":"10.1038/s44328-024-00008-9","DOIUrl":"10.1038/s44328-024-00008-9","url":null,"abstract":"We introduce a digital inline holography (DIH) method combined with deep learning (DL) for real-time detection and analysis of bacteria in liquid suspension. Specifically, we designed a prototype that integrates DIH with fluorescence imaging to efficiently capture holograms of bacteria flowing in a microfluidic channel, utilizing the fluorescent signal to manually identify ground truths for validation. We process holograms using a tailored DL framework that includes preprocessing, detection, and classification stages involving three specific DL models trained on an extensive dataset that included holograms of generic particles present in sterile liquid and five bacterial species featuring distinct morphologies, Gram stain attributes, and viability. Our approach, validated through experiments with synthetic data and sterile liquid spiked with different bacteria, accurately distinguishes between bacteria and particles, live and dead bacteria, and Gram-positive and negative bacteria of similar morphology, all while minimizing false positives. The study highlights the potential of combining DIH with DL as a transformative tool for rapid bacterial analysis in clinical and industrial settings, with potential extension to other applications including pharmaceutical screening, environmental monitoring, and disease diagnostics.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00008-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142041759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors 利用有机电化学晶体管对 PEDOT:PSS 进行功能化，以实现基于适配体的 IL6 检测

npj Biosensing Pub Date : 2024-07-24 DOI: 10.1038/s44328-024-00007-w

Bernhard Burtscher, Chiara Diacci, Anatolii Makhinia, Marios Savvakis, Erik O. Gabrielsson, Lothar Veith, Xianjie Liu, Xenofon Strakosas, Daniel T. Simon

{"title":"Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors","authors":"Bernhard Burtscher, Chiara Diacci, Anatolii Makhinia, Marios Savvakis, Erik O. Gabrielsson, Lothar Veith, Xianjie Liu, Xenofon Strakosas, Daniel T. Simon","doi":"10.1038/s44328-024-00007-w","DOIUrl":"10.1038/s44328-024-00007-w","url":null,"abstract":"Here we propose a strategy to functionalize poly(ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) based organic electrochemical transistors (OECTs) for sensing the inflammatory cytokine interleukin 6 (IL6). For this aim we use diazonium chemistry to couple 4-aminobenzoic acid to sulfonate moieties on the PSS, which can act as anchors for aptamers or other recognition elements (e.g., fluorescent, or redox probes). We investigated this approach with a commercial screen-printable PEDOT:PSS formulation but also studied the effect of PEDOT to PSS ratio as well as the amount of crosslinker in other PEDOT:PSS formulations. For screen printed OECTs, it was possible to distinguish between IL6 and bovine serum albumin (BSA) in buffer solution and detect IL6 when added in bovine plasma in the nanomolar range. Furthermore, functionalization of PEDOT:PSS formulations with higher PSS content (compared to the “standard” solutions used for OECTs) combined with frequency dependent measurements showed the potential to detect IL6 concentrations below 100 pM.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00007-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141810110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0