npj Biosensing最新文献

Direct observation of small molecule activator binding to single PR65 protein. 直接观察小分子激活剂与单个PR65蛋白的结合。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-01-16 DOI: 10.1038/s44328-024-00018-7

Annie Yang-Schulz, Maria Zacharopoulou, Sema Zeynep Yilmaz, Anupam Banerjee, Satyaki Saha, Daniel Nietlispach, Michael Ohlmeyer, Mert Gur, Laura S Itzhaki, Ivet Bahar, Reuven Gordon

{"title":"Direct observation of small molecule activator binding to single PR65 protein.","authors":"Annie Yang-Schulz, Maria Zacharopoulou, Sema Zeynep Yilmaz, Anupam Banerjee, Satyaki Saha, Daniel Nietlispach, Michael Ohlmeyer, Mert Gur, Laura S Itzhaki, Ivet Bahar, Reuven Gordon","doi":"10.1038/s44328-024-00018-7","DOIUrl":"10.1038/s44328-024-00018-7","url":null,"abstract":"The reactivation of heterotrimeric protein phosphatase 2A (PP2A) through small molecule activators is of interest to therapeutic intervention due to its dysregulation, which is linked to chronic conditions. This study focuses on the PP2A scaffold subunit PR65 and a small molecule activator, ATUX-8385, designed to bind directly to this subunit. Using a label-free single-molecule approach with nanoaperture optical tweezers (NOT), we quantify its binding, obtaining a dissociation constant of 13.6 ± 2.5 μM, consistent with ensemble fluorescence anisotropy results but challenging to achieve with other methods due to low affinity. Single-molecule NOT measurements reveal that binding increases optical scattering, indicating PR65 elongation. This interpretation is supported by all-atom molecular dynamics simulations showing PR65 adopts more extended conformations upon binding. This work highlights NOT's utility in quantifying binding kinetics and structural impact, offering insights valuable for drug discovery.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A method for blood pressure hydrostatic pressure correction using wearable inertial sensors and deep learning. 一种基于可穿戴惯性传感器和深度学习的血压静压校正方法。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-01-31 DOI: 10.1038/s44328-024-00021-y

David A M Colburn, Terry L Chern, Vincent E Guo, Kennedy A Salamat, Daniel N Pugliese, Corey K Bradley, Daichi Shimbo, Samuel K Sia

{"title":"A method for blood pressure hydrostatic pressure correction using wearable inertial sensors and deep learning.","authors":"David A M Colburn, Terry L Chern, Vincent E Guo, Kennedy A Salamat, Daniel N Pugliese, Corey K Bradley, Daichi Shimbo, Samuel K Sia","doi":"10.1038/s44328-024-00021-y","DOIUrl":"10.1038/s44328-024-00021-y","url":null,"abstract":"Cuffless noninvasive blood pressure (BP) measurement could enable early unobtrusive detection of abnormal BP patterns, but when the sensor is placed on a location away from heart level (such as the arm), its accuracy is compromised by variations in the position of the sensor relative to heart level; such positional variations produce hydrostatic pressure changes that can cause swings in tens of mmHg in the measured BP if uncorrected. A standard method to correct for changes in hydrostatic pressure makes use of a bulky fluid-filled tube connecting heart level to the sensor. Here, we present an alternative method to correct for variations in hydrostatic pressure using unobtrusive wearable inertial sensors. This method, called IMU-Track, analyzes motion information with a deep learning model; for sensors placed on the arm, IMU-Track calculates parameterized arm-pose coordinates, which are then used to correct the measured BP. We demonstrated IMU-Track for BP measurements derived from pulse transit time, acquired using electrocardiography and finger photoplethysmography, with validation data collected across 20 participants. Across these participants, for the hand heights of 25 cm below or above the heart, mean absolute errors were reduced for systolic BP from 13.5 ± 1.1 and 9.6 ± 1.1 to 5.9 ± 0.7 and 5.9 ± 0.5 mmHg, respectively, and were reduced for diastolic BP from 15.0 ± 1.0 and 11.5 ± 1.5 to 6.8 ± 0.5 and 7.8 ± 0.8, respectively. On a commercial smartphone, the arm-tracking inference time was ~134 ms, sufficiently fast for real-time hydrostatic pressure correction. This method for correcting hydrostatic pressure may enable accurate passive cuffless BP monitors placed at positions away from heart level that accommodate everyday movements.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical biosensors for diagnosing neurodegenerative diseases. 诊断神经退行性疾病的光学生物传感器。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-05-02 DOI: 10.1038/s44328-025-00040-3

Khuong Duy Mac, Judith Su

引用次数: 0

A passive blood separation sensing platform for point-of-care devices. 一种用于即时护理设备的被动血液分离传感平台。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-05-02 DOI: 10.1038/s44328-025-00038-x

Cameron Gilroy, Callum D Silver, Casper Kunstmann-Olsen, Lisa M Miller, Steven D Johnson, Thomas F Krauss

{"title":"A passive blood separation sensing platform for point-of-care devices.","authors":"Cameron Gilroy, Callum D Silver, Casper Kunstmann-Olsen, Lisa M Miller, Steven D Johnson, Thomas F Krauss","doi":"10.1038/s44328-025-00038-x","DOIUrl":"https://doi.org/10.1038/s44328-025-00038-x","url":null,"abstract":"The blood test is one of the most performed investigations in clinical practice, with samples typically analysed in a centralised laboratory. Many of these tests monitor routine conditions that would benefit from a point-of-care approach, reducing the burden on practitioners, patients and healthcare systems. Such a decentralised model requires the development of sophisticated, yet easy-to-use technology; however, platforms that combine high-performance with low-cost and simplicity remain scarce. Moreover, most research papers only address a subset of requirements and study specific aspects in isolation. A systems approach that considers the interplay between each element of the technology is clearly required to develop a coherent solution. Here, we present such a systems approach in the context of testing for C-reactive protein (CRP), a commonly requested test in clinical practise that indicates inflammation and is particularly relevant for monitoring patients with chronic diseases, e.g. those with rheumatoid arthritis or who are undergoing cancer therapy. The approach we take here features an entirely passive microfluidic cartridge for blood separation, integrated with a high-performance sensing platform which we have tested in a real-world context. The device is compatible with a handheld detection unit and is simple to use yet can accurately detect CRP levels at clinically relevant levels.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"19"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI in SERS sensing moving from discriminative to generative. SERS 传感中的人工智能从判别型转向生成型。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-02-21 DOI: 10.1038/s44328-025-00033-2

Steven M Quarin, Der Vang, Ruxandra I Dima, George Stan, Pietro Strobbia

引用次数: 0

A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens. 同日α-突触核蛋白RT-QuIC种子扩增法检测突触核蛋白病生物标本。

npj Biosensing Pub Date : 2025-01-01 Epub Date: 2025-02-11 DOI: 10.1038/s44328-024-00023-w

Sabiha Parveen, Parvez Alam, Christina D Orrù, Sarah Vascellari, Andrew G Hughson, Wen-Quan Zou, Thomas G Beach, Geidy E Serrano, David S Goldstein, Bernardino Ghetti, Giovanni Cossu, Giada Pisano, Beatrice Pinna, Byron Caughey

{"title":"A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens.","authors":"Sabiha Parveen, Parvez Alam, Christina D Orrù, Sarah Vascellari, Andrew G Hughson, Wen-Quan Zou, Thomas G Beach, Geidy E Serrano, David S Goldstein, Bernardino Ghetti, Giovanni Cossu, Giada Pisano, Beatrice Pinna, Byron Caughey","doi":"10.1038/s44328-024-00023-w","DOIUrl":"10.1038/s44328-024-00023-w","url":null,"abstract":"Parkinson's disease (PD), dementia with Lewy bodies (DLB), and other synucleinopathies are characterized by the accumulation of abnormal, self-propagating aggregates of α-synuclein. RT-QuIC or seed amplification assays are currently showing unprecedented diagnostic sensitivities and specificities for synucleinopathies even in prodromal phases years in advance of the onset of Parkinsonian signs or dementia. However, commonly used α-synuclein seed amplification assays take ≥48 h to perform as applied to patients' diagnostic biospecimens. Here, we report the development of a faster α-synuclein RT-QuIC assay that is as analytically sensitive as prior assays of this type, but can be completed in ≤12 h for brain, skin, and intestinal mucosa, with positive signals often arising in <5 h. CSF assays took a few hours longer. Our same-day α-synuclein RT-QuIC (sdRT-QuIC) assay should increase the practicality, cost-effectiveness, and throughput of measurements of pathological forms of α-synuclein for fundamental research, clinical diagnosis, and therapeutics development.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":"2 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of electrical impedance spectroscopy of bovine eyes for early detection of uveal melanoma 牛眼电阻抗谱对葡萄膜黑色素瘤早期检测的评价

npj Biosensing Pub Date : 2024-12-27 DOI: 10.1038/s44328-024-00022-x

Vaibhav B. Yadav, Enosh Lim, Alison H. Skalet, Mohammad J. Moghimi

引用次数: 0

Fully automated sample to result SIMPLE RPA microfluidic chip towards in ovo sexing application 全自动化采样结果SIMPLE RPA微流控芯片在蛋类检测中的应用

npj Biosensing Pub Date : 2024-12-26 DOI: 10.1038/s44328-024-00019-6

Simão Monteiro Belo dos Santos, Celine Wegsteen, Dries Vloemans, Matthias Corion, Bart De Ketelaere, Dragana Spasic, Jeroen Lammertyn

{"title":"Fully automated sample to result SIMPLE RPA microfluidic chip towards in ovo sexing application","authors":"Simão Monteiro Belo dos Santos, Celine Wegsteen, Dries Vloemans, Matthias Corion, Bart De Ketelaere, Dragana Spasic, Jeroen Lammertyn","doi":"10.1038/s44328-024-00019-6","DOIUrl":"10.1038/s44328-024-00019-6","url":null,"abstract":"Several European countries have implemented legislations to eliminate day-old male chicks killing. Although embryo sexing (in ovo sexing) is the most promising alternative, no current solution can handle all egg colors with >98% sexing accuracy, low cost and minimal embryo disturbance before day 13 of incubation and processing >20,000 eggs/hour. Recombinase polymerase amplification (RPA) presents a promising alternative to PCR that can be integrated into microfluidic platforms. In this work, we developed fully autonomous microfluidic cartridge (SIMPLE-RPA chip) for female chick-specific synthetic HINTW gene detection in 30 min at 37.7 °C inside an egg incubator. We first optimized off-chip RPA, allowing for highly sensitive DNA detection (1.6 × 10–5 ng/µL). The SIMPLE-RPA chip was developed to automate the RPA bioassay on-chip, reducing user errors, and contamination risks and maintaining the off-chip LOD while offering low price, small footprint, upscaling compatibility, and easy transfer to other point-of-care applications.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00019-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements of paper-based sensors for antibiotic-resistant bacterial species identification 纸张传感器在耐药细菌种类鉴定中的应用进展

npj Biosensing Pub Date : 2024-12-13 DOI: 10.1038/s44328-024-00016-9

Aayushi Laliwala, Ashruti Pant, Denis Svechkarev, Marat R. Sadykov, Aaron M. Mohs

引用次数: 0

A quantitative, label-free visual interference colour assay platform for protein targeting and binding assays 一个定量的，无标签的视觉干扰颜色分析平台，用于蛋白质靶向和结合分析

npj Biosensing Pub Date : 2024-12-05 DOI: 10.1038/s44328-024-00017-8

Ana Lopez-Campistrous, Hillary Sweet, Ciaran Terry, Craig Garen, Yu Wan, Robert E. Burrell, Kyle Moxham, Matthew Nickel, Michael Serpe, Michael Joyce, Lorne Tyrrell, Todd P. W. McMullen

{"title":"A quantitative, label-free visual interference colour assay platform for protein targeting and binding assays","authors":"Ana Lopez-Campistrous, Hillary Sweet, Ciaran Terry, Craig Garen, Yu Wan, Robert E. Burrell, Kyle Moxham, Matthew Nickel, Michael Serpe, Michael Joyce, Lorne Tyrrell, Todd P. W. McMullen","doi":"10.1038/s44328-024-00017-8","DOIUrl":"10.1038/s44328-024-00017-8","url":null,"abstract":"The vast array of immunoassay technologies used to assess protein interactions is costly or platform-specific. We present a label-free visual interference colour assay (VICA) that quantifies peptide and protein interactions by creating an iridescent surface allowing direct visualisation without spectrophotometric optics or microfluidics. A nanoporous aluminium oxide surface is tuned to match the refractive indices of the overlying protein layers to generate visual interference colours. To functionalise the surface, we created an affinity-capture system using a protein A-carboxyglutamic (GLA) construct that orients antibodies to enhance the signal. Using off-the-shelf antibodies, the platform can isolate analytes in buffer, whole blood, or serum. This surface generates a discernible colour change at concentrations as low as 50 femtomoles/mm2 and can monitor oligomer formation in sequential steps on the same slide. VICA provides comparable kinetic parameters to biolayer interferometry and traditional immunoassays while also allowing characterisation of proteins in large macromolecular complexes.","PeriodicalId":501705,"journal":{"name":"npj Biosensing","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44328-024-00017-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0