bioRxiv - Microbiology最新文献

A system for functional studies of the major virulence factor of malaria parasites 疟疾寄生虫主要毒力因子功能研究系统

bioRxiv - Microbiology Pub Date : 2024-09-19 DOI: 10.1101/2024.04.30.591946

Jakob Cronshagen, Johannes Allweier, Paolo Mesen-Ramirez, Jan Staecker, Anna Viktoria Vaaben, Gala Ramon-Zamorano, Isabel Naranjo, Susann Ofori, Pascal WTC Jansen, Joelle Hornebeck, Florian Kieferle, Agnes Murk, Elicia Martin, Carolina Castro-Pena, Richard Bartfai, Thomas Lavstsen, Iris Bruchhaus, Tobias Spielmann

{"title":"A system for functional studies of the major virulence factor of malaria parasites","authors":"Jakob Cronshagen, Johannes Allweier, Paolo Mesen-Ramirez, Jan Staecker, Anna Viktoria Vaaben, Gala Ramon-Zamorano, Isabel Naranjo, Susann Ofori, Pascal WTC Jansen, Joelle Hornebeck, Florian Kieferle, Agnes Murk, Elicia Martin, Carolina Castro-Pena, Richard Bartfai, Thomas Lavstsen, Iris Bruchhaus, Tobias Spielmann","doi":"10.1101/2024.04.30.591946","DOIUrl":"https://doi.org/10.1101/2024.04.30.591946","url":null,"abstract":"PfEMP1 is a variable antigen displayed on erythrocytes infected with the malaria parasite Plasmodium falciparum. PfEMP1 mediates binding of the infected cell to the endothelium of blood vessels, a cause of severe malaria. Each parasite encodes ~60 different PfEMP1 variants but only one is expressed at a time. Switching between variants underlies immune evasion in the host and variant-specific severity of disease. PfEMP1 is difficult to study due to expression heterogeneity between parasites which also renders genetic modification approaches ineffective. Here, we used selection linked integration (SLI) to generate parasites all expressing the same PfEMP1 variant and genome edit the expressed locus. Moving this system from the reference strain 3D7 to IT4 resulted in PfEMP1 expressor parasites with effective receptor binding capacities. We also introduce a second version of SLI (SLI2) to introduce additional genome edits. Using these systems, we study PfEMP1 trafficking, generate cell lines binding to all major endothelial receptors, survey the protein environment from functional PfEMP1 in the host cell and identify new proteins needed for PfEMP1 mediated sequestration. These findings show the usefulness of the system to study the key virulence factor of malaria parasites.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes 合成乳清蛋白和牛乳清蛋白的蛋白质组和 N-聚糖比较及其对人体肠道微生物群的影响

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.18.613515

Matthew Bolino, Hatice Duman, Izzet Avci, H Mehmet Kayili, Juli Petereit, Chandler Zundel, Bekir Salih, Sercan Karav, Steven A Frese

{"title":"Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes","authors":"Matthew Bolino, Hatice Duman, Izzet Avci, H Mehmet Kayili, Juli Petereit, Chandler Zundel, Bekir Salih, Sercan Karav, Steven A Frese","doi":"10.1101/2024.09.18.613515","DOIUrl":"https://doi.org/10.1101/2024.09.18.613515","url":null,"abstract":"Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives to traditional agricultural sources. At the same time, a deeper understanding of how dietary components interact with the gut microbiome has highlighted the importance of understanding the nuances underpin-ning diet-microbiome interactions. Novel food proteins with distinct post-translational modifications resulting from their respective production systems have not been characterized, nor how they may differ from their traditionally produced counterparts. To address this, we have characterized the pro-tein composition and N-glycome of a yeast-synthesized whey protein ingredient isolated from com-mercially available ice cream and compared this novel ingredient to whey protein powder isolate derived from bovine milk. We found that despite strong similarities in protein composition, the N-glycome significantly differs between these protein sources, reflecting the biosynthetic machinery of the production systems. Further, the composition profile and diversity of proteins found in the syn-thetic whey protein were lower relative to bovine whey protein, despite both being predominantly composed of β-lactoglobulin. Finally, to understand whether these differences in N-glycome profiles affected the human gut microbiome, we tested these proteins in an in vitro fecal fermentation model. We found that the two whey protein sources generated significant differences among three distinct microbial compositions, which we hypothesize is a product of differences in N-glycan composition and degradation by these representative microbial communities. This work highlights the need to understand how differences in novel biotechnological systems affect the bioactivity of these pro-teins, and how these differences impact the human gut microbiome.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Characterization of Bronchial and Nasal Mucus Reveals Key Determinants of Influenza A Virus Inhibition 支气管粘液和鼻腔粘液的特性比较揭示了抑制甲型流感病毒的关键因素

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.17.613498

Marie O Pohl, Kalliopi Violaki, Lu Liu, Elisabeth Gaggioli, Irina Glas, Josephine von Kempis, Chia-wei Lin, Céline Terrettaz, Shannon C. David, Frank Charlton, Ghislain Motos, Nir Bluvshtein, Aline Schaub, Liviana K Klein, Beiping Luo, Walter Hugentobler, Ulrich K Krieger, Thomas Peter, Tamar Kohn, Athanasios Nenes, Silke Stertz

{"title":"Comparative Characterization of Bronchial and Nasal Mucus Reveals Key Determinants of Influenza A Virus Inhibition","authors":"Marie O Pohl, Kalliopi Violaki, Lu Liu, Elisabeth Gaggioli, Irina Glas, Josephine von Kempis, Chia-wei Lin, Céline Terrettaz, Shannon C. David, Frank Charlton, Ghislain Motos, Nir Bluvshtein, Aline Schaub, Liviana K Klein, Beiping Luo, Walter Hugentobler, Ulrich K Krieger, Thomas Peter, Tamar Kohn, Athanasios Nenes, Silke Stertz","doi":"10.1101/2024.09.17.613498","DOIUrl":"https://doi.org/10.1101/2024.09.17.613498","url":null,"abstract":"Differentiated primary human respiratory epithelial cells grown at air-liquid interface have become a widely used cell culture model of the human conducting airways. These cultures contain secretory cells such as goblet and club cells, which produce and secrete mucus. Here, we characterize the composition of mucus harvested from airway cultures of nasal and bronchial origin. We find that despite inter-donor variability, the salt, sugar, lipid, and protein content and composition are very similar between nasal and bronchial mucus. However, subtle differences in the abundance of individual components in nasal versus bronchial mucus can influence its antimicrobial properties: The ability of mucus to neutralize influenza A virus varies with the anatomical origin of the airway cultures and correlates with the abundance of triglycerides and sialylated glycoproteins and glycolipids.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single ancestral ANP32 locus in ticks creates multiple protein variants that all support the Thogoto virus polymerase 蜱虫的一个祖先 ANP32 基因座产生了多种蛋白质变体，它们都支持 Thogoto 病毒聚合酶

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.16.613268

Zhenyu Zhang, Thomas Nipper, Ishmael D Aziati, Adrianus Boon, Andrew Mehle

引用次数: 0

Cap-independent translation directs stress-induced differentiation of the protozoan parasite Toxoplasma gondii 依赖于帽子的翻译指导了应激诱导的弓形虫原生寄生虫的分化

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.17.613578

Vishakha Dey, Michael J Holmes, Matheus S Bastos, Ronald C Wek, William J Sullivan

{"title":"Cap-independent translation directs stress-induced differentiation of the protozoan parasite Toxoplasma gondii","authors":"Vishakha Dey, Michael J Holmes, Matheus S Bastos, Ronald C Wek, William J Sullivan","doi":"10.1101/2024.09.17.613578","DOIUrl":"https://doi.org/10.1101/2024.09.17.613578","url":null,"abstract":"Translational control mechanisms modulate microbial latency of eukaryotic pathogens, enabling them to evade immunity and drug treatments. The protozoan parasite Toxoplasma gondii persists in hosts by differentiating from proliferative tachyzoites to latent bradyzoites, which are housed inside tissue cysts. Transcriptional changes facilitating bradyzoite conversion are mediated by a Myb domain transcription factor called BFD1, whose mRNA is present in tachyzoites but not translated into protein until stress is applied to induce differentiation. We addressed the mechanisms by which translational control drives BFD1 synthesis in response to stress-induced parasite differentiation. Using biochemical and molecular approaches, we show that the 5-leader of BFD1 mRNA is sufficient for preferential translation upon stress. The translational control of BFD1 mRNA is maintained when ribosome assembly near its 5-cap is impaired by insertion of a 5-proximal stem-loop and upon knockdown of the Toxoplasma cap-binding protein, eIF4E1. Moreover, we show that a trans-acting RNA-binding protein called BFD2/ROCY1 is necessary for cap-independent translation of BFD1 through its binding to the 5-leader. Translation of BFD2 mRNA is also suggested to be preferentially induced under stress, but by a cap-dependent mechanism. These results show that translational control and differentiation in Toxoplasma proceed through cap-independent mechanisms in addition to canonical cap-dependent translation. Our identification of cap-independent translation in protozoa underscores the antiquity of this mode of gene regulation in cellular evolution and its central role in stress-induced life-cycle events.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of the inoculum size on the in vivo activity of the aztreonam-avibactam combination in a murine model of peritonitis due to Escherichia coli expressing CTX-M-15 and NDM-1 在表达 CTX-M-15 和 NDM-1 的大肠埃希菌腹膜炎小鼠模型中，接种体大小对阿兹曲南-阿维菌素复方制剂体内活性的影响

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.18.613740

Laura Benchetrit, Ariane Amoura, Samuel Chosidow, Alice Le Menestrel, Victoire de Lastours, Francoise Chau, Sara Dion, Laurent Massias, Bruno Fantin, Agnes Lefort

{"title":"Impact of the inoculum size on the in vivo activity of the aztreonam-avibactam combination in a murine model of peritonitis due to Escherichia coli expressing CTX-M-15 and NDM-1","authors":"Laura Benchetrit, Ariane Amoura, Samuel Chosidow, Alice Le Menestrel, Victoire de Lastours, Francoise Chau, Sara Dion, Laurent Massias, Bruno Fantin, Agnes Lefort","doi":"10.1101/2024.09.18.613740","DOIUrl":"https://doi.org/10.1101/2024.09.18.613740","url":null,"abstract":"Background: The combination of aztreonam (ATM) and avibactam (AVI) is an attractive option to treat infections caused by extended spectrum 𝛽-lactamase plus NDM-1-producing Enterobacteriaceae. Since ATM activity was shown to be severely impacted by an increase in the inoculum size in vitro, we wondered whether ATM-AVI activity could be impaired in high inoculum infections. Methods: We analyzed the impact of the inoculum size on ATM-AVI activity in vitro and in a murine model of peritonitis due to susceptible E. coli CFT073-pTOPO and its isogenic derivatives producing NDM-1 (E. coli CFT073-NDM1) and CTX-M-15 plus NDM-1 (E. coli CFT073-CTXM15-NDM1). The impact of the inoculum size on bacterial morphology was studied by microscopic examination.\u0000Results: In vitro, at standard (105) inoculum, E. coli CFT073-CTXM15-NDM1 was resistant to ATM but susceptible to the ATM-AVI combination. At high (107) inoculum, MICs of ATM alone and of the ATM-AVI combination reached > 512 and 64 mg/L respectively, against all tested strains. ATM led to bacterial filamentation when active against the bacteria, i.e., in monotherapy or in combination with AVI against susceptible E. coli CFT073-pTOPO, and only in combination with AVI against E. coli CFT073-CTXM15-NDM1. In vivo, increase in the inoculum led to a drastic decrease in the activity of ATM alone against E. coli CFT073-pTOPO, and of ATM-AVI against E. coli CFT073-CTXM15-NDM1. Conclusion: Our results suggest a high in vivo impact of the inoculum increase on the activity of ATM alone against ATM-susceptible E. coli, and of ATM-AVI against CTX-M-15 plus NDM-1 producing E. coli. Clinicians must be aware of the risk of failures when using AZT-AVI in high inoculum infections.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chronic murine model of pulmonary Acinetobacter baumannii infection enabling the investigation of late virulence factors, long-term antibiotic treatments, and polymicrobial infections 一种慢性鼠肺鲍曼不动杆菌感染模型，可用于研究晚期毒力因子、长期抗生素治疗和多微生物感染

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.17.613469

Clay D. Jackson-Litteken, Gisela Di Venanzio, Manon Janet-Maitre, Ítalo de Araujo Castro, Joseph J. Mackel, David A Rosen, Carolina B Lopez, Mario F Feldman

{"title":"A chronic murine model of pulmonary Acinetobacter baumannii infection enabling the investigation of late virulence factors, long-term antibiotic treatments, and polymicrobial infections","authors":"Clay D. Jackson-Litteken, Gisela Di Venanzio, Manon Janet-Maitre, Ítalo de Araujo Castro, Joseph J. Mackel, David A Rosen, Carolina B Lopez, Mario F Feldman","doi":"10.1101/2024.09.17.613469","DOIUrl":"https://doi.org/10.1101/2024.09.17.613469","url":null,"abstract":"Acinetobacter baumannii can cause prolonged infections that disproportionately affect immunocompromised populations. Our understanding of A. baumannii respiratory pathogenesis relies on an acute murine infection model with limited clinical relevance that employs an unnaturally high number of bacteria and requires the assessment of bacterial load at 24-36 hours post-infection. Here, we demonstrate that low intranasal inoculums in immunocompromised mice with a tlr4 mutation leads to reduced inflammation, allowing for persistent infections lasting at least 3 weeks. Using this chronic infection model, we determined the adhesin InvL is an imperative virulence factor required during later stages of infection, despite being dispensable in the early phase. We also demonstrate that the chronic model enables the distinction between antibiotics that, although initially reduce bacterial burden, either lead to complete clearance or result in the formation of bacterial persisters. To illustrate how our model can be applied to study polymicrobial infections, we inoculated mice with an active A. baumannii infection with Staphylococcus aureus or Klebsiella pneumoniae. We found that S. aureus exacerbates the infection, while K. pneumoniae enhances A. baumannii clearance. In all, the chronic model overcomes some limitations of the acute pulmonary model, expanding our capabilities to study of A. baumannii pathogenesis and lays the groundwork for the development of similar models for other important opportunistic pathogens.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-harvesting by antenna-containing rhodopsins in pelagic Asgard archaea 浮游阿斯加德古菌中含触角的罗多普勒蛋白的光收集作用

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.18.613612

Gali Tzlil, Maria del Carmen Marin, Yuma Matsuzaki, Probal Nag, Shota Itakura, Yosuke Mizuno, Shunya Murakoshi, Tatsuki Tanaka, Shirley Larom, Masae Konno, Rei Abe-Yoshizumi, Ana Molina-Marquez, Daniela Barcenas-Perez, Jose Cheel, Michal Koblizek, Rosa Leon, Kota Katayama, Hideki Kandori, Igor Schapiro, Wataru Shihoya, Osamu Nureki, Keiichi Inoue, Andrey Rozenberg, Ariel Chazan, Oded Beja

{"title":"Light-harvesting by antenna-containing rhodopsins in pelagic Asgard archaea","authors":"Gali Tzlil, Maria del Carmen Marin, Yuma Matsuzaki, Probal Nag, Shota Itakura, Yosuke Mizuno, Shunya Murakoshi, Tatsuki Tanaka, Shirley Larom, Masae Konno, Rei Abe-Yoshizumi, Ana Molina-Marquez, Daniela Barcenas-Perez, Jose Cheel, Michal Koblizek, Rosa Leon, Kota Katayama, Hideki Kandori, Igor Schapiro, Wataru Shihoya, Osamu Nureki, Keiichi Inoue, Andrey Rozenberg, Ariel Chazan, Oded Beja","doi":"10.1101/2024.09.18.613612","DOIUrl":"https://doi.org/10.1101/2024.09.18.613612","url":null,"abstract":"Aquatic bacterial rhodopsin proton pumps have been recently reported to utilize hydroxylated carotenoids1,2. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we report on light energy transfer from diverse hydroxylated carotenoids (lutein, diatoxanthin, and fucoxanthin) to heimdallarchaeial rhodopsins (HeimdallRs)3,4 from uncultured marine planktonic members of the 'Ca. Kariarchaeaceae' ('Ca. Asgardarchaeota')5. These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light absorbing retinal chromophore within HeimdallRs. Furthermore, antenna enhancement of proton pumping by HeimdallRs is also observed under white-light illumination along with a carotenoid-binding induced structural change in the protein. Our results indicate that the use of light-harvesting antennas in microbial rhodopsins is observed not only in bacteria but also in marine archaea.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"214 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WITHDRAWN: Interspecies recombination between cassava brown streak disease-causing viruses 撤回：木薯褐条病致病病毒之间的种间重组

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.08.06.606877

Keryan Astacio-Berrios, Siobain Duffy

引用次数: 0

Systematic genome-guided discovery of antagonistic interactions between archaea and bacteria 在基因组指导下系统发现古细菌与细菌之间的拮抗相互作用

bioRxiv - Microbiology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.18.613068

Romain Strock, Valerie W.C. Soo, Antoine Hocher, Tobias Warnecke

{"title":"Systematic genome-guided discovery of antagonistic interactions between archaea and bacteria","authors":"Romain Strock, Valerie W.C. Soo, Antoine Hocher, Tobias Warnecke","doi":"10.1101/2024.09.18.613068","DOIUrl":"https://doi.org/10.1101/2024.09.18.613068","url":null,"abstract":"The social life of archaea is poorly understood. In particular, even though competition and conflict are common themes in microbial communities, there is scant evidence documenting antagonistic interactions between archaea and their abundant prokaryotic brethren: bacteria. Do archaea specifically target bacteria for destruction? If so, what molecular weaponry do they use? Here, we present an approach to infer antagonistic interactions between archaea and bacteria from genome sequence. We show that a large and diverse set of archaea encode peptidoglycan hydrolases, enzymes that recognize and cleave a structure - peptidoglycan - that is a ubiquitous component of bacterial cell walls but absent from archaea. We predict the bacterial targets of archaeal peptidoglycan hydrolases using a structural homology approach and demonstrate that the predicted target bacteria tend to inhabit a similar niche to the archaeal producer, indicative of ecologically relevant interactions. Using a heterologous expression system, we demonstrate that two peptidoglycan hydrolases from the halophilic archaeaon Halogranum salarium B-1 kill the halophilic bacterium Halalkalibacterium halodurans, a predicted target, and do so in a manner consistent with peptidoglycan hydrolase activity. Our results suggest that, even though the tools and rules of engagement remain largely unknown, archaeal-bacterial conflicts are likely common, and we present a roadmap for the discovery of additional antagonistic interactions between these two domains of life. Our work has implications for understanding mixed microbial communities that include archaea and suggests that archaea might represent a large untapped reservoir of novel antibacterials.","PeriodicalId":501357,"journal":{"name":"bioRxiv - Microbiology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0