Journal of Medical Research最新文献

{"title":"The Dual Role of ZEB2 in COAD Metastasis and Immunology","authors":"Jingjing Wang, Bin Lu, Simin Zeng, Liqing Li, Huiyan Zhuo, Youqiang Li","doi":"10.6913/mrhk.050101","DOIUrl":"https://doi.org/10.6913/mrhk.050101","url":null,"abstract":"Objective: The zinc finger E-box binding homeobox (ZEB2), which can accelerate the nuclear DNA replication by inducing the activation of upstream transcription promoters, was widely considered as an oncogene. Recent study has found that the overexpression of ZEB2 is associated with a better prognosis in hepatocellular carcinoma. However, its roles in tumor growth, metastasis, and immunology are yet to be elucidated in COAD. Methods: The pan-cancer sequencing data was acquired from The Cancer Genome Atlas (TCGA)-Pan cancer cohort, normal human tissue data was acquired from the Genotype-tissue expression (GTEx) database, and Broad Institute Cancer Cell Line Encyclopedia (CCLE) were downloaded from UCSC Xena. We used the cBioPortal webtool to analyze and visualize the ZEB2 pan-cancer genomic alteration rate. GEO Expression Datasets were used to explore ZEB2 expression levels in COAD patients. UCSC Xena database was used to download prognostic information of COAD patients. The Cox regression and Kaplan–Meier analyses were used to assess the prognostic role of ZEB2 in COAD. Kyoto Encyclopedia of Genes and Genomes (KEGG) was performed to determine the biological pathways. Gene Ontology (GO) enrichment analysis was performed to determine the biological processes, molecular functions, and cellular components that were altered in a ZEB2-dependent manner in COAD. The module analysis of PPI interaction network was performed using the MCODE tool of Cytoscape software, and the characteristic molecules were selected by cytohHubba tool. CIBERSORTx database was used to analyze the ZEB2 expression in the presence of 22 types of immune infiltrating cells. Results: This study found that ZEB2 was aberrantly expressed in most cancer types, and it was significantly downregulated in COAD compared with normal tissue. In addition, our findings also show that overexpression of ZEB2 was associated with a better prognosis in COAD. Mechanistic analysis revealed that overexpression of ZEB2 was associated with the neutrophil extracellular trap formation in COAD. And the results show that ZEB2 expression was significantly correlated with several kinds of immune cell infiltration. Conclusion: This study demonstrates that overexpression of ZEB2 was associated with better prognoses in patients with COAD. ZEB2 has close relationship with ACTB, which was highly related to NETs. These findings suggest a dual role of ZEB2 in COAD growth, metastasis, and immunology.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75937409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Bowel Preparation for Capsule Endoscopy and Colonoscopy: A Patient-Centered Protocol Revision and Application","authors":"Haiping Yan, Linmin Lai, Hongxiang Chen","doi":"10.6913/mrhk.050105","DOIUrl":"https://doi.org/10.6913/mrhk.050105","url":null,"abstract":"Objective: This study aimed to evaluate the effectiveness of a modified patient experience-based bowel preparation protocol for capsule endoscopy combined with colonoscopy. Methods: A total of 262 patients who underwent capsule endoscopy combined with colonoscopy in a tertiary hospital in Zhuhai city from July to December 2021 were enrolled in this study. They were divided into the control group (n=141) and the experimental group (n=121) using the random number method. The experimental group used the modified bowel preparation protocol while the control group used the conventional preparation protocol. Post-examination indicators, including sleep quality, gastrointestinal discomfort, nausea, vomiting, abdominal pain, and satisfaction, were compared between the two groups. Results: The data showed that the experimental group’s preparation medication had a better taste and was effective in improving patient symptoms, such as gastrointestinal discomfort, nausea and vomiting, abdominal pain, and sleep quality, compared to the control group. Moreover, both patients and nurses reported greater satisfaction with the experimental group’s bowel preparation time compared to the control group, with statistically significant differences observed (P < 0.05). Conclusions: The modified bowel preparation protocol could improve patient sleep quality, reduce adverse reactions, and increase patient comfort and satisfaction.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76374101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Lactulose for Colonoscopy Preparation in Adults:A Meta-Analysis","authors":"Weiwen Shi, Jiapeng Zhang, Ping Liu","doi":"10.6913/mrhk.050104","DOIUrl":"https://doi.org/10.6913/mrhk.050104","url":null,"abstract":"Objective: To evaluate the effects of lactulose for bowel preparation prior to colonoscopy in adults. Methods: By searching for literature pertaining to RCT (randomized controlled trial) of bowel preparation with lactulose in adults up to 10th of July, 2021. The experimental group utilizes lactulose oral solution as the solution of choice for bowel preparation while the control group uses the standard bowel preparation solution. Literature selection and data extraction were completed independently by 2 researchers, including literature quality control. Data analysis was completed using the software RevMan5.2. Results: 11 RCT was included for data analysis. Meta-analysis showed that the lactulose group had a lower incidence of adverse effects [OR=13.58, 95CI% (6.10, 30.24), p<0.0001] and better tolerability [OR=13.58, 95CI% (6.10,30.24), p<0.0001] when compared with the control group. However, the bowel cleanliness was statistically insignificant between the two groups [WMD=0.26，95%CI (-0.31，0.82), p=0.38]. Conclusion: according to current evidences, as a solution for bower preparation, lactulose has a lower incidence of adverse effects such as emesis or diarrhea, while effectively increasing tolerability, however it is not a superior solution for bowel preparation in terms of bowel cleanliness when compared with other standard choices. More high quality researches will be required to support its use.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82422814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of sarcosine in urine as a predictor of prostate cancer using Enzyme linked immunosorbent method","authors":"Mukaa M Januaris, W. Njoroge, A. Radol, Jeremiah Gathirwa Waweru","doi":"10.31254/jmr.2023.9102","DOIUrl":"https://doi.org/10.31254/jmr.2023.9102","url":null,"abstract":"Prostate cancer is a type of malignancy that is defined by abnormal development of cells in the prostate tissue. Prostate cancer needs early intervention since its incidence and prevalence is high across the world leading to high morbidity and mortality. Prostatic specific antigen test which is the commonly used screening test in Kenya and across the world is nonspecific, expensive and inaccessible to many people in rural setting who are in need. The definitive histological test is invasive and requires specialized facilities and personnel. This study sought to investigate sarcosine in urine as a predictor of prostate cancer to supplement prostatic specific antigen test in the diagnosis of prostate carcinoma. Cross sectional study design was employed in this study for all suspected prostate cancer identified according to clinical assessment during the study period. Midstream urine samples of about 30mls was collected in plastic tubes, centrifuged and supernatant collected and analyzed using ELISA method for sarcosine. Raw data obtained was tabulated in excel and transferred to statistical package for social science. Differences in means and standard deviation from various age groups was analyzed using one-way Anova and Independent t test. The Bonferroni was used as post Hoc to test the means that were significant from others. Significance level was set at 95%. The concentration of sarcosine (4.30±0.11nmol/ml) in prostate cancer participants was significantly higher than the concentration (0.47±0.06nmol/ml) of control participants using ELISA (p<0.001;). Hence Sarcosine in urine needs to be analyzed for the testing of prostate tumor since it is raised in confirmed prostate carcinoma participants as compared to negative control units. The age groups of the prostate tumor participants had no significant variation in sarcosine concentration using ELISA method (p=0.57). Similarly, the age groups of the control individuals were not significantly different in sarcosine concentration (p=0.17). Future studies need to dwell in incorporating sarcosine metabolite in urine.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42923582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HSF1: a main aim in treatment of cancers","authors":"J. Ayatollahi, S. H. Khaleghinejad, Ghazaleh Harirzadeh, Marzieh Lotfi, Mahdie Hamidfar, S. Shahcheraghi, Mohammad Dehghanniri, Azam Fazilati, Samad Sadeghi Bakh","doi":"10.31254/jmr.2023.9101","DOIUrl":"https://doi.org/10.31254/jmr.2023.9101","url":null,"abstract":"HSF1 is a main regulator factor associated with several proteins especially HSPs. It is also a factor with important role in tumor severity, and can be a biomarker in the diagnostic investigations. This Letter discusses about role of HSF1 as a therapeutic aim in cancer.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42107331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The epidemiological situation of brucellosis in Isfahan province (2021)","authors":"J. Ayatollahi, Sadaf Shafiee, S. Mousavi, Mahdie Hamidfar, S. Shahcheraghi","doi":"10.31254/jmr.2023.9104","DOIUrl":"https://doi.org/10.31254/jmr.2023.9104","url":null,"abstract":"Brucellosis is one of the most important health and economic challenges of many developing countries, including Iran. The present study was conducted in the cities covered by Isfahan University of Medical Sciences and Health Services with the aim of investigating the epidemiology of brucellosis in Isfahan province. Our research was a cross-sectional study on all people suffering from brucellosis disease who were identified in Isfahan province during 2021. Data was analyzed using appropriate statistical methods and by SPSS. In the present study, most of the brucellosis patients were men. Most of them lived in rural areas. The age groups of 30-40 years and less than 20 years included the most cases of patients. Planning to empower at-risk groups in facing risk factors and strengthening the care system are important in the prevention and control of brucellosis.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46843599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urethrocystoscopy in the Urology Department of the Zinvie La Croix Hospital AU Benin: Indications and Results","authors":"Basirwa Musengo Fiston, Djialiri Malick, Muhindo Lutegha, M. Moïse, Gandji Franck, Avakoudjo Déjinnin Josué Georges","doi":"10.31254/jmr.2023.9103","DOIUrl":"https://doi.org/10.31254/jmr.2023.9103","url":null,"abstract":"Background: Urethrocystoscopy is a basic endoscopic examination in urology. The aim of this study was to describe the indications and results obtained during urethrocystoscopy in patients received and treated in the urology department of the Zinvie La Croix Hospital in Benin. Patients and methods: It was a prospective descriptive study conducted in the urology department of the Zinvie La Croix Hospital from January 1st, 2022 to July 30th, 2022, involving 92 patients. The variables studied were: age, sex, indication for urethrocystoscopy and results obtained. Results: Of the 92 patients collected, there were 72 men (78.2%) and 20 women (21.7%); the sex ratio was 3.6. The age of the patients ranged from 16 to 80 years with an average of 43.43 years; 48.8% of the patients were under 40 years of age and the age group (20-39 years) represented 43.4%. The main indications were lower urinary tract symptoms (48.57%) followed by hematuria (19.04%). The main results were bladder neck sclerosis 14 (17.28%) and urethral stricture 13 (16.04%) in men and urinary bilharzia 8 (40%) and bladder tumor 4 (20%) in women. Conclusion: Urethrostoscopy is a primary examination in urology. Lower urinary tract symptoms were the main indication; bladder neck sclerosis in men and urinary bilharzia in women were the main pathologies encountered. This technique should be popularized in urological outpatient surgery.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44856951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of animal bite cases in Kerman city","authors":"J. Ayatollahi, Rahim Jam, S. Mousavi, Tahereh Fallah, Mahdie Hamidfar, S. Shahcheraghi","doi":"10.31254/jmr.2022.8604","DOIUrl":"https://doi.org/10.31254/jmr.2022.8604","url":null,"abstract":"Rabies is one of the most important common diseases between humans and animals and is considered as one of health problems in the world. This study was conducted to investigate the epidemiological characteristics of animal bite cases referred to rabies prevention centers in Kerman city. The current study was cross-sectional. All registered cases of animal bites at the rabies prevention centers in Kerman were investigated during 2021. The data of the study was analyzed according to demographic and epidemiological information using descriptive and inferential statistics methods. The results of the study showed that there were 3460 cases of animal bites and its prevalence was higher in young people and males. The most common animal bites were caused by dogs. Considering the many cases of animal bites in Kerman, it is recommended to plan educational and care activities to reduce them.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45975074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pattern of antibiotic sensitivity of gram-negative MDR bacteria in patients admitted to the ICU","authors":"Zohre Akhondimeybodi, Mahya Sadat Emami Meybodi, S. Mousavi, Mahdie Hamidfar, S. Shahcheraghi","doi":"10.31254/jmr.2022.8603","DOIUrl":"https://doi.org/10.31254/jmr.2022.8603","url":null,"abstract":"Due to the uncontrolled increase in the administration of antibiotics, the prevalence of multidrug-resistant organisms in patients admitted to intensive care units has also increased. Thus, this study aimed to investigate the pattern of sensitivity and compliance of treatment with the results of culture in gram-negative MDR bacteria in patients admitted to the intensive care unit of Shahid Sadoughi Hospital in Yazd. This cross-sectional study was performed over a period of one year on the results of culture of patients admitted to the intensive care unit of Shahid Sadoughi Hospital in Yazd (2020). The samples included blood, urine, wounds, bronchoalveolar lavage, shunt, and throat. All patient information and antibiotics prescribed were obtained using their records. Finally, the data was entered into SPSS (v. 26) and analyzed. In total, out of 298 patients for whom initial antibiotics were started experimentally, 226 cases had the culture results consistent with one of the antibiotics that were started initially. Among the antibiotics that were used more, clindamycin showed the least agreement with antibiotic sensitivity results. Among antibiotics, clindamycin had significantly the most compatibility in blood and urine and the least compatibility in wound. The present study showed a relatively good agreement in the initial experimental administration of antibiotics.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47982830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wnt: A Main Protein in Bladder Cancer","authors":"J. Ayatollahi, Ghazaleh Harirzadeh, Marzieh Lotfi, Mahdie Hamidfar, S. Shahcheraghi, Mohammad Dehghanniri, Azam Fazilati, Samad Sadeghi Bakh","doi":"10.31254/jmr.2022.8601","DOIUrl":"https://doi.org/10.31254/jmr.2022.8601","url":null,"abstract":"Bladder cancer (BC) is one of the most common cancers in the world. Wnt/b-catenin is one of involved signaling pathways in this cancer. This letter aimed to discuss about the importance of Wnt in BC.","PeriodicalId":50132,"journal":{"name":"Journal of Medical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43210883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}