medRxiv - Gastroenterology最新文献

{"title":"Efficacy and safety of 10-day versus 14-day bismuth-containing quadruple therapy for H. pylori eradication: A systematic review and meta-analysis","authors":"Najam Gohar, Zoya Ejaz, Faizan Ahmed, Abdul Rafay, Abdullah Humayun, Momna Nisar, Ali Mushtaq, Aanusha Ghouri, Fatima Zafar, Hira Khalid, Sania Afzal, Muhammad Hammad Khan, Huzaifa Ahmad Cheema, Muhammad Shahzil, Essam Rashad, Rehmat Ullah Awan, Prasun K Jalal","doi":"10.1101/2024.08.18.24312061","DOIUrl":"https://doi.org/10.1101/2024.08.18.24312061","url":null,"abstract":"Background\u0000Nearly half of the world population is infected by Helicobacter pylori (H. pylori). Bismuth-containing quadruple therapy (BQT) has shown favorable outcomes. This study compares 10-day and 14-day BQT regimens to evaluate their efficacy, safety, and compliance rates. Methods\u0000We searched electronic databases from their inception until May 2024 to retrieve all randomized controlled trials (RCTs) that compared 10-day and 14-day BQT regimens for H. pylori eradication. Meta-analysis was performed using Review Manager 5.4. Dichotomous outcomes were compared using risk ratio (RR). Results\u0000Seven RCTs and a total of 2,424 patients were included in the meta-analysis. There was no significant difference in the intention-to-treat eradication rate (RR 0.97; 95% CI 0.94, 1.01) and the per-protocol eradication rate (RR 0.96; 95% CI 0.93, 1.00) between the 10-day BQT and 14-day BQT groups. Commonly reported adverse events in both groups were epigastric pain and discomfort, nausea, and vomiting. There was no significant difference in the risk of adverse events between the two groups (RR 0.80; 95% CI 0.63, 1.02). There was no significant difference in the compliance rate between the two groups (RR 1.02; 95% CI 1.00, 1.04).\u0000Conclusion The eradication rates, risk of adverse events, and compliance rates were comparable between the two groups. Future research comparing similar drug doses with larger sample sizes and longer patient follow-ups can improve the quality of results.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thomasclavelia ramosa is a Signature of Gut Dysbiosis associated with Alcohol-Related Hepatocellular Carcinoma: A First Microbial Culturomics Study","authors":"Reham Magdy Wasfy, Anissa ABDOULAYE, Patrick BORENTAIN, Babacar MBAYE, Maryam TIDJANI ALOU, Aurelia CAPUTO, Claudia ANDRIEU, Giovanna MOTTOLA, Anthony LEVASSEUR, Matthieu Million, Rene GEROLAMI","doi":"10.1101/2024.08.19.24312231","DOIUrl":"https://doi.org/10.1101/2024.08.19.24312231","url":null,"abstract":"Background: Gut microbiota alteration is implicated in the pathogenesis of alcoholic liver disease (ALD) and HCC. No study has characterized the dysbiosis associated with ALD by microbial culturomics, an approach that certifies viability and allows the characterization of pathobiont strain candidates. Methods: A case-control study was conducted on patients with ALD without HCC (ALD-NoHCC) (n=16), ALD with HCC (ALD-HCC) (n=19), and controls (n=24). 16S rRNA amplicon sequencing and microbial culturomics were used as complementary methods for gut microbiome profiling. Results: By microbial culturomics, Thomasclavelia ramosa was the most enriched and detected in all ALD samples (100%), while it was cultivated in only a small proportion of controls (20%, p < 0.001). By 16S rRNA amplicon sequencing and 3-groups linear discriminant analysis, T. ramosa was increased explicitly in the ALD-HCC group (LDA-score > 5, p < 0.05). Conclusions: T. ramosa, identified by culturomics and 16 rRNA sequencing, is associated with ALD and ALD-HCC. Alongside the recently reported in vitro genotoxicity of this species in colorectal cancer, this species has been identified as a candidate oncobiont in ALD-HCC.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metabolic constraint in the kynurenine pathway drives mucosal inflammation in IBD","authors":"Lina Welz, Danielle Harris, Na-Mi Kim, Abrar Alsaadi, Qcong Wu, Mhmd Oumari, Jan Taubenheim, Valery Volk, Graziella Credido, Eric Koncina, Pranab Mukherjee, Florian Tran, Laura Katharina Sievers, Polychronis Pavlidis, Nicholas Powell, Florian Rieder, Elisabeth Letellier, Silvio Waschina, Christoph Kaleta, Friedrich Feuerhake, Bram Verstockt, Melanie McReynolds, Philip Rosenstiel, Stefan Schreiber, Konrad Aden","doi":"10.1101/2024.08.08.24311598","DOIUrl":"https://doi.org/10.1101/2024.08.08.24311598","url":null,"abstract":"Inflammatory bowel disease (IBD) is associated with perturbed metabolism of the essential\u0000amino acid tryptophan (Trp). Whether increased degradation of Trp directly fuels mucosal\u0000inflammation or acts as a compensatory attempt to restore cellular energy levels via de-novo\u0000nicotinamide adenine dinucleotide (NAD+) synthesis is not understood. Employing a systems\u0000medicine approach on longitudinal IBD therapy intervention cohorts and targeted screening in\u0000preclinical IBD models, we discover that steady increases in Trp levels upon therapy success\u0000coincide with a rewiring of metabolic processes within the kynurenine pathway (KP). In detail,\u0000we identify that Trp catabolism in IBD is metabolically constrained at the level of quinolinate\u0000phosphorybosyltransferase (QPRT), leading to accumulation of quinolinic acid (Quin) and a\u0000decrease of NAD+. We further demonstrate that Trp degradation along the KP occurs locally\u0000in the inflamed intestinal mucosa and critically depends on janus kinase / signal transducers\u0000and activators of transcription (JAK/STAT) signalling. Subsequently, knockdown of QPRT invitro\u0000induces NAD+ depletion and a pro-inflammatory state, which can largely be rescued by\u0000bypassing QPRT via other NAD+ precursors. We hence propose a model of impaired de-novo\u0000NAD+ synthesis from Trp in IBD. These findings point towards the replenishment of NAD+\u0000precursors as a novel therapeutic pathway in IBD.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IBS stress reactivity phenotype is associated with blood transcriptome profiles and microstructural and functional brain changes","authors":"Jennifer Labus, Desiree Delgadillo, Steve Cole, Chencai Wang, Bruce Naliboff, Lin Chang, Benjamin Ellingson, Emeran Mayer","doi":"10.1101/2024.08.07.24311369","DOIUrl":"https://doi.org/10.1101/2024.08.07.24311369","url":null,"abstract":"Background & Aims: Clinical evidence suggests significant interindividual differences in stress reactivity (SR), but biological mechanisms and therapeutic implications of these differences are poorly understood.\u0000We aimed to identify the biological basis of increased SR by investigating associations between a psychometric-based phenotype with blood transcriptomics profiles of increased sympathetic nervous system (SNS) activation and brain imaging phenotypes in irritable bowel syndrome (IBS) participants and healthy controls (HCs). Methods: A cross-sectional observational study design, transcriptomics profiling, multimodal brain imaging, and psychosocial assessments were obtained in 291 female and male IBS participants and HCs. Prior to analyses, unsupervised clustering was applied to derive high and low SR subgroups across participants based on two measures of SR. General linear models tested for SR group differences in clinical and biological parameters. Exploratory analyses examined associations between SR group-specific brain alterations and gene expression. Results: The high, compared to low SR group showed greater cyclic AMP response element-binding protein (CREB) gene expression consistent with tonic SNS activity and proinflammatory changes in whole blood. Brain imaging showed neuroplastic changes in the high SR group consistent with an upregulation of ascending arousal systems and sensory processing and integration regions, and functional connectivity changes in the central autonomic network. SR moderated the sex difference in extraintestinal symptoms. Conclusions: The findings support a model of tonically increased SNS activity as a plausible risk factor for increased autonomic reactivity to psychosocial stressors and low grade immune activation in both IBS and HCs, with a greater prevalence in IBS. These findings may have important implications for personalized treatment interventions in IBS.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal Hematopoiesis of Indeterminate Potential in Crohn′s Disease and Ulcerative Colitis","authors":"Myvizhi Esai Selvan, Daniel I Nathan, Daniela Guisado, Giulia Collatuzzo, Sushruta Iruvanti, Paolo Boffetta, John Mascarenhas, Ronald Hoffman, Louis J Cohen, Bridget K Marcellino, Zeynep H Gümüş","doi":"10.1101/2024.08.06.24311497","DOIUrl":"https://doi.org/10.1101/2024.08.06.24311497","url":null,"abstract":"Background\u0000Clonal hematopoiesis of indeterminate potential (CHIP) is the presence of somatic mutations in myeloid and lymphoid malignancy genes in the blood cells of individuals without a hematologic malignancy. Inflammation is hypothesized to be a key mediator in the progression of CHIP to hematologic malignancy and patients with CHIP have a high prevalence of inflammatory diseases. This study aimed to identify the prevalence and characteristics of CHIP in patients with inflammatory bowel disease (IBD). Methods\u0000We analyzed whole exome sequencing data from 587 Crohn′s disease (CD), 441 ulcerative colitis (UC), and 293 non-IBD controls to assess CHIP prevalence and used logistic regression to study associations with clinical outcomes. Results\u0000Older UC patients (age>45) harbored increased myeloid-CHIP mutations compared to younger patients (age≤45) (p=0.01). Lymphoid-CHIP was more prevalent in older IBD patients (p=0.007). Young CD patients were found to have myeloid-CHIP with high-risk features. IBD patients with CHIP exhibited unique mutational profiles compared to controls. Steroid use was associated with increased CHIP (p=0.05), while anti-TNF therapy was associated with decreased myeloid-CHIP (p=0.03). Pathway enrichment analyses indicated overlap between CHIP genes, IBD phenotypes, and inflammatory pathways. Conclusions\u0000Our findings underscore a connection between IBD and CHIP pathophysiology. Patients with IBD and CHIP had unique risk profiles especially among older UC patients and younger CD patients. These findings suggest distinct evolutionary pathways for CHIP in IBD and necessitate awareness among IBD providers and hematologists to identify patients potentially at risk for CHIP-related complications including malignancy, cardiovascular disease and acceleration of their inflammatory disease.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Exome Sequencing Reveals FCGBP Variant Associated with Spontaneous Intraabdominal Hemorrhage in Severe Acute Pancreatitis","authors":"Qiuyi Tang, Yue-Peng Hu, Qi Yang, Jing Zhou, Jing-Zhu Zhang, Jie Yang, Haibin Hao, Gang Li, Bai-Qiang Li, Lu Ke, Zhi-Hui Tong, Yu-Xiu Liu, Evan Yi-Wen Yu, Wei-Qin Li","doi":"10.1101/2024.08.06.24311443","DOIUrl":"https://doi.org/10.1101/2024.08.06.24311443","url":null,"abstract":"This study sought to identify genetic cause of spontaneous intraabdominal hemorrhage (SIH) in severe acute pancreatitis (SAP) to develop more effective treatment for this life-threatening complication. A four-phase study was conducted, leveraging a large-scale acute pancreatitis (AP) patients (n=600); the first phase involved whole-exome sequencing analyses, and identified specific exonic variant located in <em>FCGBP</em> (i.e., rs1326680184) that was consistently associated with SIH; the second phase performed serum ELISA tests, and revealed that <em>FCGBP</em> variant altered FCGBP level and further led to predisposition of SIH; the third phase conducted an i) <em>in-vivo</em> experiment with a <em>Fcgbp</em>-knockdown mouse model, and demonstrated lower expression of <em>Fcgbp</em> led to more severe AP morphology and higher risk of hemorrhage; ii) <em>in-vitro</em> experiment with <em>FCGBP</em>-knockdown human vascular fibroblasts demonstrated that down-regulated <em>FCGBP</em> expression could destabilize the vascular wall, and lead to vascular injury in SAP; the fourth phase compared <em>FCGBP</em> variant carriers to non-carriers with clinical characteristics, and found <em>FCGBP</em> variant associated with higher risks of poor complications and AP prognosis and enhanced the diagnostic capability as an indicator. These findings provide important insights into the underlying mechanism of SIH in SAP, and facilitate therapeutic development for AP prognosis and critical care in an early phase.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of celiac disease on a gluten-free diet: a multicenter prospective quasi-experimental clinical study","authors":"Sara Gomez-Aguililla, Sergio Farrais, Natalia Lopez-Palacios, Beatriz Arau, Carla Senosiain, Maria Corzo, Nora Fernandez-Jimenez, Angela Ruiz-Carnicer, Fernando Fernandez-Bañares, Barbara P Gonzalez-Garcia, Eva Tristan, Ana Montero-Calle, Maria Garranzo-Asensio, Isabel Casado, Mar Pujals, Juana Maria Hernandez, Jorge Infante-Menendez, Garbiñe Roy, Carolina Sousa, Concepcion Nuñez","doi":"10.1101/2024.08.05.24311406","DOIUrl":"https://doi.org/10.1101/2024.08.05.24311406","url":null,"abstract":"Background\u0000Diagnosing celiac disease (CD) in individuals adhering to a gluten-free diet (GFD) presents significant challenges. Current guidelines recommend a gluten challenge (GC) lasting at least 6-8 weeks, which has several limitations.\u0000Objectives\u0000This study compares four approaches previously proposed for diagnosing CD on a GFD: IL-2 serum levels, gut-homing CD8⁺ T cells, %TCRγδ⁺ intraepithelial lymphocytes (IELs), and UBE2L3 gene expression. Additionally, we evaluated the CD8⁺ T-cell based method with a 3-day GC against the standard GC protocol. Methods\u0000We conducted a multicenter prospective quasi-experimental clinical study. Two subsets of individuals were considered: 1) 20 patients with CD and 15 non-CD controls previously diagnosed, to evaluate the first aim; 2) 45 individuals with uncertain diagnosis who were on a GFD and required GC following current clinical guidelines, to assess the second aim. All participants underwent a 3-day GC (10 g gluten/day).\u0000Results\u0000Among CD patients and non-CD controls, the sensitivity and specificity of IL-2, gut-homing CD8⁺ T cells, and UBE2L3 were 82.4% and 83.3%, 88.2% and 100%, and 52.9% and 100%, respectively. The percentage of TCRγδ⁺ IELs showed 88.2% sensitivity. In the uncertain diagnosis group, a CD8+ T-cell positive response was observed in 8 of the 45 subjects. Conclusion\u0000The percentage of TCRγδ⁺ IELs and the gut-homing CD8⁺ T-cell assay are promising diagnostic methods for CD on a GFD. Notably, the CD8+ T-cell assay provides a consistent and reliable alternative to the extended GC, eliminating the need for the invasive procedures to obtain duodenal samples and the prolonged gluten ingestion.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Efficacy of Brush Cytology and Forceps Biopsy in the Diagnosis of Malignant Biliary Strictures: A Single-Center Study in Pakistan","authors":"Abdullah Khalid, Sara Qureshi, Imran Ali Syed, Hassan Nadeem, Ahmad Karim Malik, Usman Aujla","doi":"10.1101/2024.08.03.24311458","DOIUrl":"https://doi.org/10.1101/2024.08.03.24311458","url":null,"abstract":"ABSTRACT Introduction: The accurate diagnosis of biliary strictures or narrowing of the bile duct is challenging in clinical practice. Differentiating malignant from benign strictures is critical, because these treatments differ considerably. Although noninvasive imaging techniques help identify these strictures, they do not provide definitive tissue diagnosis, making techniques such as brush cytology and forceps biopsy essential. This study aimed to address the dearth of comparative research on the diagnostic efficacy of brush cytology and forceps biopsy in Pakistan. Methods: This single-center observational study was conducted at the Pakistan Kidney and Liver Institute &amp; Research Center (PKLI&amp;RC), Lahore, from March 2019 to January 2023. Patients with clinically and radiologically suspected biliary strictures were included in the study. Both brush cytology and forceps biopsy samples were subjected to cytopathological analysis by blinded pathologists. Sensitivity, specificity, and predictive values were among the key metrics analyzed. Results: The study included 54 patients in the biopsy group and 89 in the brushing group. In terms of diagnostic metrics, the biopsy technique displayed a sensitivity of 65.8%, specificity of 92.3%, positive predictive value (PPV) of 96.4%, and accuracy rate of 72.2%. For the brushing technique, the sensitivity, specificity, PPV, and accuracy were 56.7 %, 93.3%, 83.1%, and 62.9%, respectively. Although both methods showed high specificity, the biopsy technique exhibited a slightly better sensitivity and overall accuracy. Conclusion: Our findings underscore the importance of the biopsy method in the accurate diagnosis of malignant biliary strictures, showing marginally superior sensitivity and overall accuracy compared to brush cytology. This knowledge can guide clinicians in Pakistan and similar settings in making informed diagnostic choices to improve patient outcomes.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovering methylation markers and development of a sense-antisense and dual-MGB probe PCR assay in plasma for colorectal cancer early detection","authors":"Yanteng Zhao, Zhijie Wang, Qiuning Yu, Xin Liu, Xue Liu, Shuling Dong, Xianping Lv, Tiao Zhang, Dihan Zhou, Qiankun Yang","doi":"10.1101/2024.07.31.24311206","DOIUrl":"https://doi.org/10.1101/2024.07.31.24311206","url":null,"abstract":"Background: Screening for colorectal cancer (CRC) using plasma cell-free DNA (cfDNA) methylation is more challenging than stool testing due to the low abundance of cfDNA. Therefore, the development of signal amplification assays based on appropriate markers is essential to increase sensitivity.\u0000Methods: A total of 17 existing 450K microarray datasets including tissue, healthy white blood cell (WBC) and plasma cfDNA data from public databases were used to identify differentially methylated CpGs (DMCs) common to CRC and adenoma. The methylation status of candidate DMCs was confirmed by Sanger sequencing with CRC and normal tissues. A sense-antisense and dual MGB probe (SADMP) assay was then developed. Subsequently, the biomarkers were validated in 712 plasma samples using the SADMP method.\u0000Results: A total of 2237 DMCs showed overlap between the cancer vs. normal and adenoma vs. normal groups. Of these, 75 were hypomethylated in 30 other non-CRC cancers. After LASSO regression, this number was reduced to eight. Two of these, NTMT1 and MAP3K14-AS1, were identified as promising candidate markers following WBC validation and primer/probe design evaluation. The SADMP technology demonstrated the ability to amplify the detection signal to approximately twice the original level. Overall, the dual-target SADMP assay demonstrated a sensitivity of 84.8% for CRC (stage I: 75.0%), a sensitivity of 32.0% for advanced adenomas (AA), and a specificity of 91.5% in controls.\u0000Conclusions: The dual-target assay demonstrated high performance for CRC and AA detection in plasma-based tests, suggesting that it may serve as a promising noninvasive tool for CRC detection.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141885013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex Differences in Colonic Mucosal Microbiome of Irritable Bowel Syndrome Patients Compared to Healthy Controls","authors":"Swapna Mahurkar-Joshi, Simer Shera, Jennifer Labus, Tien S Dong, Jonathan P Jacobs, Lin Chang","doi":"10.1101/2024.07.31.24311317","DOIUrl":"https://doi.org/10.1101/2024.07.31.24311317","url":null,"abstract":"Background: Irritable bowel syndrome (IBS) is a female-predominant disorder of brain-gut\u0000interactions. Our previous study on colonic mucosal microbiota demonstrated significant differences between IBS bowel habit subtypes and showed that gut microbiota is associated with abdominal pain in IBS patients. However, there is no consensus on sex-related differences in mucosal microbiota in IBS compared to healthy controls (HC). We aimed to identify sex-related differences in the mucosal microbes associated with IBS. Methods: Sigmoid mucosal biopsies were obtained from 97 Rome+ IBS patients and 54 healthy controls (HC). Mucosal microbiome was characterized using 16S rRNA sequencing and analyzed and general linear models were used to test group differences between IBS diagnosis and sex. Sex-specific relationships between mucosal microbiome and IBS symptoms were assessed using sparse partial least squares (sPLS) regression. Results: Beta diversity was significantly different between men and women overall (p=.03) but not within IBS or HC. IBS women showed lower abundance of Catenibacterium and Ruminoclstridium_9 and increased abundance of Bacteroides, Escherichia/Shigella, Lachnoclostridium and Ruminococcaceae compared to men with IBS (p&lt;0.05). However, healthy women had a lower abundance of six distinct genera compared to healthy men. In women, higher IBS symptoms were associated with an increased abundance of bacteria including prevotella_9, and paraprevotella, however, in men, IBS symptoms were associated with increased abundances of genera such as Dialister. Interestingly, increased abundance of Desulfovibrio was associated with higher symptoms in women but lower symptoms in men. Conclusion: There are distinct sex-related differences in the mucosal microbiome between IBS and healthy participants supporting the importance of studying sex-specific mechanisms in IBS pathophysiology.","PeriodicalId":501258,"journal":{"name":"medRxiv - Gastroenterology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141885087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}