Journal of Muscle Foods最新文献

{"title":"EFFECTS OF ADDED CRUDE GREEN TEA POLYPHENOL ON THE LIPID OXIDATION OF COMMON CARP (CYPRINUS CARPIO L.) AND CATFISH (CLARIAS GARIEPINUS BURCHELL) DURING REFRIGERATED STORAGE","authors":"S. DEMBELE,&nbsp;D.F. WANG,&nbsp;L.N. YU,&nbsp;J.P. SUN,&nbsp;S.Y. DONG","doi":"10.1111/j.1745-4573.2010.00216.x","DOIUrl":"10.1111/j.1745-4573.2010.00216.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The antioxidant activity of crude green tea polyphenol (GTP) on the lipid oxidation of carp (</i>Cyprinus carpio <i>L.) and catfish (</i>Clarias gariepinus <i>Burchell) was investigated. Each fish was trimmed to remove scales, head, fins, tail, viscera, bones, visible fat and skin, and treated with different concentrations of GTP 0.01, 0.03, 0.05 and 0.01% of vitamin C (VC). Control muscle sample (CK) contained no antioxidant. Lipid oxidation was evaluated over a course of 9 days and using a 3-day periodical interval analysis for peroxide value, thiobarbituric acid-reactive substances and free fatty acid. Carp minced fillet containing 0.03% GTP showed the best characteristics (</i>P &lt; <i>0.05) in relation to oxidative stability. For catfish, 0.05% GTP concentration was more effective in delaying lipid oxidation, when compared with the control sample and VC. However, 0.01%-treated minced fillet with VC was less effective than 0.01% GTP in reducing lipid oxidation. These results demonstrate that tea polyphenols inhibited lipid oxidation, and are effective alternatives to VC in food industry.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Green tea polyphenol (GTP) is a natural antioxidant. It can improve the quality of food products during refrigerated storage because of the presence of catechin in its composition. GTP promotes health by preventing lipid oxidation, and has been shown to possess antibacterial and antiviral characteristics. In food industry, it can be used as alternatives to the synthetic antioxidants because the use of these types of antioxidants is controlled nonetheless because of their carcinogenic potential.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00216.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN EVALUATION OF THE ANTIOXIDANT AND ANTIMICROBIAL EFFECTIVENESS OF DIFFERENT FORMS OF GARLIC AND BHA IN EMULSION-TYPE SAUSAGES DURING REFRIGERATED STORAGE","authors":"Y.J. KIM,&nbsp;B.A. NAHM,&nbsp;I.H. CHOI","doi":"10.1111/j.1745-4573.2010.00221.x","DOIUrl":"10.1111/j.1745-4573.2010.00221.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The antioxidant and antimicrobial effects of each of different forms of garlic and BHA in emulsion-type sausage at 4C for 0, 10, 20 and 30 days were investigated. The treatments were divided into five groups: control, 30 g of fresh garlic /kg of sausage, 9 g of garlic powder /kg of sausage, 100 mL of garlic extract/kg of sausage and 0.1 g of BHA/kg of sausage. pH, peroxide value (POV), thiobarbituric acid reactive substance (TBARS), residual nitrite contents (RN) and total aerobic plate count (TAPC) were evaluated during storage. pH values and RN contents showed a significant decrease in all treatments during storage days. With the exception of POV value at 30 days of storage, an increase in POV, TBARS and TAPCs was observed in all treatments as storage time increased. In addition, the treatment with different forms of garlic and BHA during storage reduced pH, POV, TBARS, RN and TAPCs when compared with control. Consequently, the use of fresh garlic had much better antioxidant and antimicrobial effectiveness than other treatments.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Antioxidant or antimicrobial effectiveness is one of the major factors related to consumer acceptance for determining the quality of meat and meat products and their shelf life. The current study can be applied to prevent lipid oxidation and growth of microorganism in emulsion-type sausage and to produce new sausage formulations for a gradually increasing consumer acceptance. Based on the results, different forms of garlic can be used to improve oxidative stability of emulsion-type sausage and decrease the growth of microorganisms proving its antioxidant and antimicrobial effectiveness. The employment of natural additives in food preparations is perceived by consumers as a healthier practice than the addition of synthetic additives.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00221.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63403137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF PROTEOLYTIC ENZYME TREATMENTS AND PRESSURE COOKING ON QUALITY OF SPENT SHEEP MEAT CURRY","authors":"S.K. MENDIRATTA,&nbsp;B.D. SHARMA,&nbsp;R. NARAYAN,&nbsp;B.G. MANE","doi":"10.1111/j.1745-4573.2010.00213.x","DOIUrl":"10.1111/j.1745-4573.2010.00213.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>This study was conducted to improve the qualities of meat curry prepared from tough sheep meat. Tenderization treatments given before or during preparation of meat curry included spraying of powder of dried cucumis fruits, marinating meat chunks in ginger rhizome paste, 0.75% citric acid solution, 0.6% papain enzyme solution or cooking of chunks in specially designed pressure cooker at 15 psi. All these tenderizing treatments caused significant improvement in sensory scores of sheep meat curry in comparison to control. Shear force and collagen values were significantly lower in all treated meat curry samples than the control. Although there was significant increase (</i>P &lt; <i>0.01) in thiobarbituric acid-reducing substance, pH and microbial load of meat curry on the fifth day of refrigerated storage, all the values were within the prescribed limits, and all samples were well acceptable. Magnitudes of changes during storage were less in citric acid- and ginger-treated chunks than the control and other treated chunks.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Different tenderizing agents suggested for tenderization of sheep meat can be easily used to improve the quality of meat curry. Conditions standardized for cooking tough meat at 15 psi proved very effective in improving the qualities of meat curry from spent animals in comparison to cooking of control samples in ordinary pressure cooker (at 10 psi). Cucumis powder proved very effective in improving all the sensory attributes. Use of citric acid and ginger extract can be recommended when meat curry is to be used after storage at refrigerated temperature.</p>\u0000 \u0000 <p>These techniques of tenderization of tough meat before or during cooking can be easily adopted at small-scale and industrial levels. Improvement in tenderness will be of great value to meat processors and consumers. Better avenues for spent sheep meat utilization would also contribute for increased returns to the farmers. The process will not involve any significant extra expenditure, equipment or technique.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00213.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FLESH QUALITY IN LARGE RAINBOW TROUT WITH HIGH OR LOW FILLET YIELD","authors":"JEROME BUGEON,&nbsp;FLORENCE LEFEVRE,&nbsp;MIREILLE CARDINAL,&nbsp;AYHAN UYANIK,&nbsp;ARMEL DAVENEL,&nbsp;PIERRICK HAFFRAY","doi":"10.1111/j.1745-4573.2010.00214.x","DOIUrl":"10.1111/j.1745-4573.2010.00214.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Rainbow trout with different fillet yield [56 and 65% for low (LY) and high yield (HY), respectively] were examined for muscle organization and flesh quality (instrumental and sensorial evaluations). Both groups had similar body weight (3.6 kg in mean), but the HY group had a higher carcass yield (</i>+<i>15%). Higher total muscle area in the HY group (</i>+<i>20%) was associated with a higher number of muscle fibers (</i>+<i>22%). Flesh of the HY group presented a higher area of myosepta (</i>+<i>10%), fat content (</i>+<i>10%) and luminosity (</i>+<i>6%). Raw fillet mechanical resistance was higher for the HY group; an opposite result was obtained for cooked flesh. Sensorial evaluation of cooked flesh revealed no important differences between groups. Smoked fillet from the HY group presented higher area of white stria and lower flesh color intensity. To conclude, higher muscle mass content had no negative consequences on flesh quality in rainbow trout.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Farmed rainbow trout presents great variability in fillet yield, and low fillet yield is considered as an unexpected carcass quality trait, as it leads to financial loss and higher volume of off-products, difficult to valorize. Therefore, the improvement of fillet yield appears as an interesting criterion for genetic selection, but only if increasing fillet yield does not result in a lower-quality product. This study provides useful information on the characteristics of the flesh of rainbow trout with different fillet yield. The results reported in this article show to fish farmers and processors that improving fillet yield has no negative impact on raw and processed flesh quality. From an academic point of view, this work provides additional knowledge in the area of the determinism of flesh quality in farmed fish.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00214.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VOLATILE PROFILE AND SENSORY CHARACTERISTICS OF DRY-CURED LOINS AS AFFECTED BY FEEDING LEVEL IN THE PERIOD PREVIOUS TO THE LATE FATTENING PHASE AND BY REARING SYSTEM OF IBERIAN PIGS","authors":"E. SOTO,&nbsp;L. HOZ,&nbsp;J.A. ORDÓÑEZ,&nbsp;E. HIERRO,&nbsp;B. HERRANZ,&nbsp;C. LÓPEZ-BOTE,&nbsp;M.I. CAMBERO","doi":"10.1111/j.1745-4573.2010.00210.x","DOIUrl":"10.1111/j.1745-4573.2010.00210.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The effect of a moderate feed restriction (50 g feed/kg LW^0.75[low feeding level, L]</i> versus <i>70 g feed/kg LW^0.75[high feeding level, H]) during the period previous to the late fattening phase on chemical composition, volatile profile and sensory quality of Iberian dry-cured loins from animals reared in free range (FR) or in confinement (C) along the fattening phase was studied. Total tocopherol, total free amino acid and ammonia contents of dry-cured loins from pigs FRL and FRH were higher (</i>P &lt; <i>0.05) than those from CL and CH. Sixty-one volatile compounds were identified and semiquantified in the headspace of all dry-cured loins. The FRL products showed the highest (</i>P &lt; <i>0.05) amino acid catabolism volatile compounds. In the rank-order test sensory analysis, the FR products obtained higher sums of ranks (</i>P &lt; <i>0.05) than those from confinement. Appearance, flavor, odor and texture scores showed a similar trend (FRL</i> ≥ <i>FRH</i> &gt; <i>CL</i> = <i>CH).</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Dry-cured products (loins and hams) from Iberian pigs that are reared at free range (FR) are highly appreciated by consumers, reaching the highest price. The feeding level of the Iberian pig in the period previous to the late fattening phase affects the amount of amino acid catabolism volatile compounds of dry-cured loins from pigs reared at FR which is reflected in a better sensorial quality of the dry-cured loin. A moderate feed restriction during the period previous to the late fattening phase of Iberian pigs reared at FR could be a good strategy to improve the sensory quality of dry-cured products.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00210.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY OF HYDROLYZED PROTEIN COMPOSITION, FREE AMINO ACID, AND TAURINE CONTENT IN DIFFERENT MUSCLES OF GALICIAN BLONDE BEEF","authors":"DANIEL FRANCO,&nbsp;LAURA GONZÁLEZ,&nbsp;ESPERANZA BISPO,&nbsp;PATRICIA RODRÍGUEZ,&nbsp;JOSÉ IGNACIO GARABAL,&nbsp;TERESA MORENO","doi":"10.1111/j.1745-4573.2010.00218.x","DOIUrl":"10.1111/j.1745-4573.2010.00218.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The aim of this study was to identify and quantify hydrolyzed and free amino acids including taurine in different muscles of the Blonde Galician breed. The main essential amino acids present in each of the muscles were lysine and leucine, with aspartic acid and glutamic being the most important in the non-essential fraction. The chemical score and the index of essential amino acids were calculated as correlation parameters of the biological value of the proteins, with the best values being obtained for the muscles</i> longisimus dorsi <i>and</i> semitendinosus<i>. An analysis was also carried out of the amino acid in the free fraction. Taurine was found to be the main free amino acid in all muscles, with the exception of the cardiac muscle, where histidine was the main one present. The muscles that presented higher amounts of taurine were the masseter muscle followed by</i> semimenbranous<i>, and</i> biceps femori<i>, where taurine represented 40, 35, and 31%, respectively, of the total content of the free fraction.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>This study provides practical and useful information on the nutritional quality of muscles, which are widely consumed in Spain. Several of these muscles are highly appreciated by the consumers in restaurants such as, eye of round, sirloin, and masseter muscle. These results will be relevant for the cattle industry, because this data add information to the fatty acid profile description of samples studied in the same breed. It will be also useful for nutritionists in describing tables of the food composition.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00218.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TENDERIZING AND PRESERVING YAK MEAT BY GINGER EXTRACT (ZINGIBER OFFICINALE ROSE)","authors":"DAI RUITONG,&nbsp;YANG ZHI,&nbsp;LI YUAN,&nbsp;LI XINGMIN,&nbsp;MA LIZHEN","doi":"10.1111/j.1745-4573.2010.00217.x","DOIUrl":"10.1111/j.1745-4573.2010.00217.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>This study was conducted to develop a method for improving tenderness and inhibiting oxidative change of yak meat by ginger extract (GE) treatment. Uniform-sized 3 cm</i> × <i>3 cm</i> × <i>3 cm yak meat chunks (Longissimus dorsi muscles) were marinated with distilled water, 3%, 5%, 7% GE and 0.2% sodium ascorbate, respectively. The samples stored for 24 h, 48 h and 72 h, and 3, 5, 7, 9 and 11 days at 4C</i> ± <i>1C. The shear force values and cooking yield of the GE-treated samples were compared with that of the control groups, and thiobarbituric acid reactive substance (TBARS) value was compared with both the control group and the 0.2% sodium ascorbate-treated group. Significantly lower shear force values and TBARS value (</i>P &lt; <i>0.05) were observed in all the GE treatment samples, and the sodium ascorbate-treated group has similar TBARS with that of the 0.3% GE group. Cooking yields were also significantly higher in the GE-treated samples than that of the control groups (</i>P &lt; <i>0.05). Scanning electron microscope photos clearly depicted the extensively broken and swollen muscle fibers in the GE-treated samples. The results indicated that 5% (v/w) GE can be effectively utilized to tenderize tough yak meat and prevent yak meat from oxidation during storage.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Yak meat is an important protein resource for the Tibetan people, but yak meat was tougher and sensitive to oxidative change. This research would have practical applications to the yak meat processing and preservation. It can be applied at the industrial and household level as an easy method to improve yak meat quality and prevent yak meat from oxidation during storage.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00217.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63403066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DETECTION OF THE INDIGENOUS SALMONELLA LEVEL IN A RADIATION-STERILIZED PURE CULTURE AND MEAT MODEL SYSTEM","authors":"DONG HO KIM,&nbsp;HYE-JEONG YUN,&nbsp;WOO-YIEL LEE,&nbsp;CHEORUN JO","doi":"10.1111/j.1745-4573.2010.00211.x","DOIUrl":"10.1111/j.1745-4573.2010.00211.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The quantitative detection of the radiation-sterilized</i> Salmonella typhimurium <i>in a pure culture and ground beef, pork and chicken meat model system was evaluated using a visual immunoprecipitate (VIP) assay kit and an enzyme-linked immunosorbent assay (ELISA) method. The VIP kit and ELISA detected 10⁵ cells/mL or above of the enriched culture of</i> S. typhimurium<i>, and the detection limit of the irradiated</i> Salmonella <i>was comparable to that of the intact cells. There was no significant difference between the detection limit of the enriched culture and that of inoculated and irradiated</i> S. typhimurium <i>in model system of ground beef, pork and chicken. Irradiation did not give any effect on the immunochemical detection of</i> S. typhimurium <i>within a dose of 5 kGy; therefore, an immunodetection method may be a simple way for the quantitative analysis of the indigenous microbes in a radiation-sterilized food.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>One of the consumers' concerns about food irradiation technology is the abuse of the technology to sterilize food products without meeting the hygienic standard such as good manufacturing practice before irradiation of food. A simple method to detect the indigenous microorganism level before irradiation process is tested as a quality assurance measure using a pure culture of <i>Salmonella typhimurium</i> and inoculated meat model system. This simple test will be helpful by providing a trust for both consumer and manufacturer to verify that their products are produced under hygienic standard before irradiation treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00211.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF METAL HYDROXIDE IMMOBILIZATION AND DNA EXTRACTION METHODS ON DETECTION OF SALMONELLA ENTERICA FROM PORK SAUSAGE BY NESTED POLYMERASE CHAIN REACTION","authors":"UDAYA SANJEEWA KUMARA RATHNAYAKA,&nbsp;SUDIP KUMAR RAKSHIT","doi":"10.1111/j.1745-4573.2010.00220.x","DOIUrl":"10.1111/j.1745-4573.2010.00220.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The aim of this work was to evaluate the effectiveness of metal hydroxide immobilization and five different DNA extraction methods in detection of</i> Salmonella enterica <i>from pork sausage samples by nested polymerase chain reaction (PCR). Immobilization of bacterial cells by Zr(OH)₄ and Ti(OH)₄ was carried out prior to DNA extraction by five DNA extraction methods. The</i> fliC <i>gene and enterotoxin (stn) gene of</i> Salmonella enterica <i>was amplified by nested PCR. Both metal hydroxide immobilization and nested PCR amplification were able to increase the detection sensitivity. DNA extraction by modified Fontana and Kapperud methods were more effective compared with other methods. The amplification of enterotoxin (stn) gene provided more sensitivity of detection compared with amplification of</i> fliC <i>gene. This study shows that the use of metal hydroxide immobilization and nested PCR are able to increase the sensitivity of</i> Salmonella enterica <i>detection from meat food samples.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATION</h3>\u0000 \u0000 <p>In this experiment, two extraction methods were found to be more effective for the extraction of DNA from <i>Salmonella enterica</i>-spiked pork sausage. Immobilization of <i>Salmonella</i> cells by metal hydroxide and application of nested polymerase chain reaction were able to increase the detection sensitivity and reduce the possibility of false positive results. These findings are useful in the selection of suitable methods for rapid, sensitive and reliable detection of bacterial pathogens from meat food samples.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00220.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63403043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF LACTATE ENHANCEMENT ON MYOGLOBIN OXYGENATION OF BEEF LONGISSIMUS STEAKS OVERWRAPPED IN PVC AND STORED AT 4C","authors":"R. RAMANATHAN,&nbsp;R.A. MANCINI,&nbsp;M.K.R. KONDA","doi":"10.1111/j.1745-4573.2010.00212.x","DOIUrl":"10.1111/j.1745-4573.2010.00212.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Injection enhancement with lactate darkens beef; however, the mechanism is unclear. Our objective was to assess the effects of lactate on beef myoglobin oxygenation. Nine strip loins were divided in half and one of three injection treatments (negative control − not injected, positive control − injected with water, and 3% lactate − injected with a water solution of lactate) was assigned to each half using an incomplete block design. Positive control and 3% lactate loins were pumped to 110% of green weight, cut into 2.54-cm thick steaks, overwrapped in polyvinyl chloride and stored at 4C. Steak surface darkening (</i>L<i>*), color stability (</i>a<i>* and metmyoglobin-reducing activity), depth of oxygen penetration and myoglobin oxygenation (1 h at 4C) were evaluated on days 0, 7 and 12 of storage. Lactate darkened color, decreased surface myoglobin oxygenation and limited oxygen penetration compared with controls (</i>P &lt; <i>0.05). Color stability was improved (</i>P &lt; <i>0.05) by lactate injection. The darkening effect of lactate may be associated with decreased myoglobin oxygenation.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Case-ready packaging has allowed beef purveyors to enhance meat with various ingredients, resulting in increased color and shelf life. Lactate is added as an antimicrobial agent; however, this beneficial effect can be somewhat diminished by lactate-induced darkening. Research assessing the mechanism(s) of lactate-induced beef color darkening is limited with previous research focused on determining the mechanism of lactate-induced color stability. The current research will give a better understanding of the role of lactate as an ingredient in beef steak darkening and, thus, provide a foundation that can be used to develop strategies to improve meat color.</p>\u0000 </section>\u0000 </div>","PeriodicalId":50122,"journal":{"name":"Journal of Muscle Foods","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4573.2010.00212.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63402337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}