Journal of Physiology-London最新文献

{"title":"Hypoxia and the cytoskeleton.","authors":"Darragh Flood, Cormac T Taylor","doi":"10.1113/JP289565","DOIUrl":"https://doi.org/10.1113/JP289565","url":null,"abstract":"<p><p>A highly-regulated and dynamic cytoskeleton is vital for functional cellular physiology and the maintenance of homeostasis. Although much is known about the mechanisms by which the cytoskeleton is regulated under physiological conditions, the effect of pathological stimuli and how this contributes to disease progression remains poorly understood. Hypoxia is a prominent microenvironmental feature of a range of pathological states including inflammation, cancer and ischaemia. In this review, we summarise what is known about the effects of hypoxia on the cytoskeleton and discuss the implications of this for physiology and disease.</p>","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurons don't work alone: The secret life of brain networks.","authors":"Ansh Chatterjee","doi":"10.1113/JP289734","DOIUrl":"https://doi.org/10.1113/JP289734","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Listen to your feet.","authors":"Vaughan G Macefield","doi":"10.1113/JP289071","DOIUrl":"https://doi.org/10.1113/JP289071","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative strategies for accelerated recovery following musculoskeletal injuries.","authors":"Koyal Garg","doi":"10.1113/JP290094","DOIUrl":"https://doi.org/10.1113/JP290094","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCK-elicited Ca²⁺ signal generation has an absolute requirement for functional intracellular receptors for IP₃, NAADP and cyclic ADP-ribose.","authors":"Ole H Petersen","doi":"10.1113/JP290045","DOIUrl":"https://doi.org/10.1113/JP290045","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory control at high altitude: The chemoreceptor as a paragon of plasticity.","authors":"Jerome A Dempsey","doi":"10.1113/JP290108","DOIUrl":"https://doi.org/10.1113/JP290108","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sound vision: MYO7A gene therapy reaches the inner ear.","authors":"Shahar Taiber, Karen B Avraham","doi":"10.1113/JP290105","DOIUrl":"https://doi.org/10.1113/JP290105","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular responses in abdominal subcutaneous adipose tissue after a session of endurance exercise: effects of exercise intensity.","authors":"Cheehoon Ahn, Tao Zhang, Thomas Rode, Gayoung Yang, Olivia K Chugh, Sierra Ellis, Sophia Ghayur, Shriya Mehta, Ryan Salzman, Hui Jiang, Stephen C J Parker, Charles F Burant, Jeffrey F Horowitz","doi":"10.1113/JP289339","DOIUrl":"https://doi.org/10.1113/JP289339","url":null,"abstract":"<p><p>The primary aim of this study was to compare the acute effects of three exercise intensities on abdominal subcutaneous adipose tissue (aSAT) transcriptome in regular exercisers. A total of 45 adults who exercise regularly were assigned to perform a single session of either low-intensity continuous (LOW; 60 min at 30% VO₂max; n = 15), moderate-intensity continuous (MOD; 45 min at 65% VO₂max; n = 15) or high-intensity interval exercise (HIGH; 10 × 1 min at 90% VO₂max interspersed with 1 min active recovery; n = 15). aSAT biopsy samples were collected before and 1.5 h after the exercise session for bulk RNA sequencing and targeted protein immunoassays. HIGH upregulated genes were involved in angiogenesis, protein secretion and insulin signalling pathways, whereas MOD and LOW upregulated genes regulated extracellular matrix (ECM) remodelling, ribosome biogenesis and oxidative phosphorylation pathways. Exercise-induced changes in aSAT clock genes, ERK protein phosphorylation and circulating cytokines were similar after all three exercise treatments. Network analysis identified exercise-responsive gene clusters linked to cardiometabolic health traits. Cell-type analysis highlighted a heterogeneous response of aSAT cell types to exercise, with distinct patterns observed across exercise intensities. Collectively, our data characterize early responses in aSAT after a single session of exercise. Because adaptations to exercise training stem from an accrual of responses after each session of exercise, these early responses to exercise are likely important contributors to the long-term structural and functional changes that occur in adipose tissue in response to exercise training. KEY POINTS: Chronic adaptations in adipose tissue from regular exercise support cardiometabolic health, but the acute molecular triggers of these adaptations remain unclear. We show that acute exercise alters gene expression, along with ERK phosphorylation in adipose tissue of regular exercisers. Exercise intensity shapes the transcriptomic response: high-intensity exercise induces inflammatory, cytokine and genes, whereas lower intensities upregulate genes involved in protein translation and oxidative phosphorylation. Network and cell-type analyses highlight intensity-specific adipose responses, revealing gene modules linked to health traits and differential engagement of adipocyte subpopulations.</p>","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is the signal in the cells? Immune cell dynamics in the pathology of acute mountain sickness.","authors":"Erica C Heinrich","doi":"10.1113/JP289747","DOIUrl":"https://doi.org/10.1113/JP289747","url":null,"abstract":"","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical and functional mapping of vagal nociceptive sensory nerve subsets innervating the mouse lower airways by intersectional genetics.","authors":"Mayur J Patil, Justin Shane Hooper, Seol-Hee Kim, Parmvir K Bahia, Nika Pavelkova, Sanjay S Nair, Teresa S Darcey, Karina V Lurye, Meghana Madaram, Jailene Fiallo, Stephen H Hadley, Thomas E Taylor-Clark","doi":"10.1113/JP289279","DOIUrl":"10.1113/JP289279","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Most vagal sensory afferents innervating the lower airways are activated by noxious stimuli including irritants (e.g. TRPV1 agonist capsaicin) and inflammatory mediators, causing nociceptive cardiorespiratory reflexes (e.g. cough, bronchospasm, changes in respiratory drive and heart rate). Vagal ganglia comprise embryologically distinct nodose and jugular neurons, but little is known of their specific contribution to nociceptive reflexes. Using a novel TRPV1&lt;sup&gt;Flp&lt;/sup&gt; mouse in combination with P2X2&lt;sup&gt;Cre&lt;/sup&gt;, Tac1&lt;sup&gt;Cre&lt;/sup&gt;, intersectional reporter mice, and adeno-associated virus we mapped and modulated distinct nociceptive afferents. TRPV1&lt;sup&gt;+&lt;/sup&gt;P2X2&lt;sup&gt;+&lt;/sup&gt; neurons were found exclusively in the nodose ganglion and were activated by αβmATP and capsaicin, but rarely expressed Tac1. TRPV1&lt;sup&gt;+&lt;/sup&gt;P2X2&lt;sup&gt;+&lt;/sup&gt; fibres innervated the lungs (many projected into the alveoli) but not the trachea. Centrally they innervated the nucleus tractus solitarius (nTS). More than 90% of TRPV1&lt;sup&gt;+&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt; neurons were found in the jugular ganglion and were activated by capsaicin but not αβmATP. TRPV1&lt;sup&gt;+&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt; fibres innervated the lungs (although none projected into the alveoli) and the trachea submucosa. They terminated solely in the paratrigeminal complex (Pa5). Many TRPV1&lt;sup&gt;-&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt; neurons were found in both nodose and jugular ganglia that innervated the trachea and large pulmonary airways. These projected to both nTS and Pa5. Using intersectional chemogenetics, we selectively stimulated lower airway afferent subsets using intravenous injections of clozapine-N-oxide. Activation of TRPV1&lt;sup&gt;+&lt;/sup&gt;, TRPV1&lt;sup&gt;+&lt;/sup&gt;P2X2&lt;sup&gt;+&lt;/sup&gt; or TRPV1&lt;sup&gt;+&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt; fibres evoked bradycardia and bradypnoea. Activation of Tac1&lt;sup&gt;+&lt;/sup&gt; fibres evoked tachycardia and tachypnoea. Activation of vagal TRPV1&lt;sup&gt;-&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt; neurons only evoked tachycardia. These data show the distinct innervation patterns and reflex function of multiple nociceptive vagal afferent subsets. KEY POINTS: Most sensory innervation of the lower airways is provided by vagal afferents, which are divided into distinct nodose and jugular subsets. Our intersectional approach showed that co-expression of P2X2 and TRPV1 labelled nodose nociceptors, that co-expression of Tac1 and TRPV1 labelled jugular nociceptors, and that Tac1 expression with TRPV1 labelled a mixed population of nodose and jugular neurons. Nodose nociceptors, jugular nociceptors and vagal TRPV1-negative Tac1-positive (TRPV1&lt;sup&gt;-&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt;) afferents project to distinct medulla subnuclei, and innervate different regions and tissues within the lower airways. Chemogenetic activation (via intravenous clozapine-N-oxide) of nodose and jugular nociceptors evoked bradypnoea and bradycardia, whereas activation of TRPV1-negative Tac1-positive (TRPV1&lt;sup&gt;-&lt;/sup&gt;Tac1&lt;sup&gt;+&lt;/sup&gt;) afferents evoked tachycardia. These data show t","PeriodicalId":50088,"journal":{"name":"Journal of Physiology-London","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}