Kaohsiung Journal of Medical Sciences最新文献_第8页

Mechanism of human chorionic gonadotropin in endometrial receptivity via the miR-126-3p/PI3K/Akt/eNOS axis. 人绒毛膜促性腺激素通过miR-126-3p/PI3K/Akt/eNOS轴影响子宫内膜容受性的机制

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12672

Wei Wang, Liang Ge, Li-Li Zhang, Li-Rong Wang, Yong-Yan Lu, Li Gou, Rui-Qiang Gou, Tong-Yu Xu, Xiao-Ling Ma, Xue-Hong Zhang

{"title":"Mechanism of human chorionic gonadotropin in endometrial receptivity via the miR-126-3p/PI3K/Akt/eNOS axis.","authors":"Wei Wang, Liang Ge, Li-Li Zhang, Li-Rong Wang, Yong-Yan Lu, Li Gou, Rui-Qiang Gou, Tong-Yu Xu, Xiao-Ling Ma, Xue-Hong Zhang","doi":"10.1002/kjm2.12672","DOIUrl":"https://doi.org/10.1002/kjm2.12672","url":null,"abstract":"Human chorionic gonadotropin (hCG) might affect endometrial receptivity, exerting integral roles in embryo implantation. This study explored the action of hCG in endometrial receptivity via the miR-126-3p/PIK3R2/PI3K/Akt/eNOS axis. The embryo implantation dysfunction (EID) mouse models were established by administrating mifepristone and human endometrial epithelial cells (EECs) were used for in vivo experiments, both followed by hCG treatment. Expression level of CD105 and protein levels of cadherin CD144 and CD146 in mice were determined by immunohistochemistry and Western blot. The levels of miR-126-3p and PIK3R2 mRNA and PIK3R2, p-PI3K p85 α, PI3K p110 α, p-Akt, Akt, p-eNOS, and eNOS protein levels were measured. Cell proliferation was evaluated by CCK-8 and EdU assays. The binding sites of miR-126-3p and PIK3R2 were predicted and verified. hCG-treated EECs were further transfected with miR-126-inhibitor for functional rescue experiments. hCG ameliorated endometrial receptivity in EID mice. Moreover, hCG promoted miR-126-3p and suppressed PIK3R2 in EID mice and EECs. miR-126-3p targeted PIK3R2. EEC proliferation was enhanced after hCG treatment but inhibited by miR-126-3p downregulation. Both in vivo and in vitro experiments validated that hCG activated the PI3K/Akt/eNOS pathway through the miR-126-3p/PIK3R2 axis. Collectively, hCG improves endometrial receptivity by activating the PI3K/Akt/eNOS pathway via regulating miR-126-3p/PIK3R2.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9435806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Formosanin C suppresses cancer cell proliferation and migration by impeding autophagy machinery. 台塑素C通过阻碍癌细胞自噬机制来抑制癌细胞的增殖和迁移。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12658

Man-Ling Chu, Pei-Wen Lin, Yu-Wen Liu, Shan-Ying Wu, Sheng-Hui Lan, Chun-Li Su, Hsiao-Sheng Liu

{"title":"Formosanin C suppresses cancer cell proliferation and migration by impeding autophagy machinery.","authors":"Man-Ling Chu, Pei-Wen Lin, Yu-Wen Liu, Shan-Ying Wu, Sheng-Hui Lan, Chun-Li Su, Hsiao-Sheng Liu","doi":"10.1002/kjm2.12658","DOIUrl":"https://doi.org/10.1002/kjm2.12658","url":null,"abstract":"Formosanin C (FC) is a natural compound extracted from Paris formosana Hayata with anticancer activity. FC induces both autophagy and apoptosis in human lung cancer cells. FC-induced depolarization of mitochondrial membrane potential (MMP) may trigger mitophagy. In this study, we clarified the effect of FC on autophagy, mitophagy, and the role of autophagy in FC-related cell death and motility. We found FC caused the continuous increase of LC3 II (representing autophagosomes) from 24 to 72 h without degradation after treatment of lung and colon cancer cells, indicating that FC blocks autophagic progression. In addition, we confirmed that FC also induces early stage autophagic activity. Altogether, FC is not only an inducer but also a blocker of autophagy progression. Moreover, FC increased MMP accompanied by overexpression of COX IV (mitochondria marker) and phosphorylated Parkin (p-Parkin, mitophagy marker) in lung cancer cells, but no colocalization of LC3 with COX IV or p-Parkin was detected under confocal microscopy. Moreover, FC could not block CCCP (mitophagy inducer)-induced mitophagy. These results imply that FC disrupts mitochondria dynamics in the treated cells, and the underlying mechanism deserves further exploration. Functional analysis reveals that FC suppresses cell proliferation and motility through apoptosis and EMT-related pathway, respectively. In conclusion, FC acts as an inducer as well as a blocker of autophagy that results in cancer cell apoptosis and decreased motility. Our findings shed the light on the development of combined therapy with FC and clinical anticancer drugs for cancer treatment.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9441076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Circular RNA circDLG1 (has_circ_0068706) functions as an oncogene in nonsmall cell lung cancer through regulating AKT/mTOR signaling and direct binding to miR-144. 环状RNA circDLG1 (has_circ_0068706)在非小细胞肺癌中作为致癌基因通过调节AKT/mTOR信号传导并直接结合miR-144发挥作用。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12662

Yong-Feng Chen, Ai-Ping Xu

{"title":"Circular RNA circDLG1 (has_circ_0068706) functions as an oncogene in nonsmall cell lung cancer through regulating AKT/mTOR signaling and direct binding to miR-144.","authors":"Yong-Feng Chen, Ai-Ping Xu","doi":"10.1002/kjm2.12662","DOIUrl":"https://doi.org/10.1002/kjm2.12662","url":null,"abstract":"Nonsmall cell lung cancer (NSCLC) is a major subtype of lung cancer, causing substantial cancer-related deaths worldwide. However, the molecular basis of NSCLC development and progression remains understudied. Recently, a circular RNA, circDLG1, has been implicated in carcinogenesis and cancer metastasis. Yet, how circDLG1 affects NSCLC progression has not been reported. Here this study aims to elucidate the role of circDLG1 in NSCLC. First, we found that circDLG1 was significantly upregulated in both the GEO dataset and NSCLC tissues. Next, we silenced the expression of circDLG1 in NSCLC cell lines. Knockdown of circDLG1 upregulated miR-144 and downregulated Protein kinase B (AKT)/mechanistic target of rapamycin (mTOR), resulting in suppression of the proliferation activity and metastasis ability of NSCLC. In addition, circDLG1 knockdown significantly decreased the expression of the mesenchymal markers, proliferating cell nuclear antigen (PCNA), and N-cadherin, while increasing the expression level of E-cadherin. In conclusion, we demonstrate that circDLG1 promotes the pathogenesis and progression of NSCLC by regulating the miR-144/AKT/mTOR signaling axis, providing potential diagnostic and therapeutic targets for designing innovative treatment strategies.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9788980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and clinical features of lymphomas involving skin in Taiwan. 台湾皮肤淋巴瘤之分布与临床特征。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12659

Cheng-Lin Wu, Su-Wei Chang, Julia Yu-Yun Lee, Tsai-Yun Chen, Chien-Chin Chen, Chia-Bao Chu, L Jeffrey Medeiros, Kung-Chao Chang

{"title":"Distribution and clinical features of lymphomas involving skin in Taiwan.","authors":"Cheng-Lin Wu, Su-Wei Chang, Julia Yu-Yun Lee, Tsai-Yun Chen, Chien-Chin Chen, Chia-Bao Chu, L Jeffrey Medeiros, Kung-Chao Chang","doi":"10.1002/kjm2.12659","DOIUrl":"https://doi.org/10.1002/kjm2.12659","url":null,"abstract":"A wide variety of primary and secondary lymphoma types involves the skin. However, reports with comparisons between both groups are limited in Taiwan. We retrospectively enrolled all cutaneous lymphomas and evaluated their clinicopathologic features. There were 221 cases of lymphoma: 182 (82.3%) primary and 39 (17.7%) secondary. Mycosis fungoides was the most common primary T-cell lymphoma, 92 (41.7%) cases, followed by CD30-positive T-cell lymphoproliferative disorders including lymphomatoid papulosis (n = 33, 14.9%) and cutaneous anaplastic large cell lymphoma (n = 12, 5.4%). The most frequent primary B-cell lymphomas were marginal zone lymphoma (n = 8, 3.6%) and diffuse large B-cell lymphoma (DLBCL), leg type (n = 8, 3.6%). DLBCL including variants was the most common secondary lymphoma involving skin. Most primary lymphomas presented at low-stage (T-cell, 86%; B-cell, 75%), whereas the majority of secondary lymphomas presented at high-stage (T-cell, 94%; B-cell, 100%). Patients with secondary lymphomas had an older mean age, more frequent B symptoms, lower serum albumin and hemoglobin, and a higher frequency of atypical lymphocytes in blood than those with primary lymphomas. In primary lymphomas, older age, lymphoma types, decreased lymphocyte counts and atypical lymphocytes in blood were poorer prognostic factors. In secondary lymphoma patients, lymphoma types, high serum lactate dehydrogenase and low hemoglobin levels predicted poorer survival. We found that the distribution of primary cutaneous lymphomas in Taiwan mirrors that of other Asian countries but shows some differences as compared with Western countries. Primary cutaneous lymphomas have a better prognosis than secondary lymphomas. Histologic classification of lymphomas highly correlated with disease presentation and prognosis.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9437069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promotive role of eukaryotic translation initiation factor 4A isoform 3 in ovarian cancer cell growth and aerobic glycolysis through the pyruvate dehydrogenase kinase 4 signaling. 真核翻译起始因子4A异构体3通过丙酮酸脱氢酶激酶4信号通路促进卵巢癌细胞生长和有氧糖酵解的作用

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12690

Sha-Sha Bai, Li-Wei Yan, Chun-Hui Liu

{"title":"Promotive role of eukaryotic translation initiation factor 4A isoform 3 in ovarian cancer cell growth and aerobic glycolysis through the pyruvate dehydrogenase kinase 4 signaling.","authors":"Sha-Sha Bai, Li-Wei Yan, Chun-Hui Liu","doi":"10.1002/kjm2.12690","DOIUrl":"https://doi.org/10.1002/kjm2.12690","url":null,"abstract":"Ovarian cancer (OC) represents one of the most detrimental gynecological malignancies. RNA-binding protein eukaryotic translation initiation factor 4A isoform 3 (EIF4A3) is well-regarded as a definitive oncogene that contributes to the development of multiple malignant tumors. This study sought to elucidate the molecular mechanism of EIF4A3 in OC growth and aerobic glycolysis by regulation of pyruvate dehydrogenase kinase 4 (PDK4) mRNA stability. We determined the EIF4A3 and PDK4 expression levels in OC cell lines and normal ovarian epithelial cells, and subsequently evaluated the cell viability and colony formation by cell counting kit-8 and colony formation assays. The degree of cell aerobic glycolysis was evaluated by measurements of lactic acid production, glucose intake, adenosine triphosphate level, extracellular oxygen consumption, and protein levels of pyruvate kinase isozymes M2 and hexokinase-2. Afterwards, we verified the binding of EIF4A3 and PDK4 mRNA via RNA immunoprecipitation, and determined the mRNA stability after actinomycin D treatment. Finally, a series of rescue experiments was performed with pcDNA3.1-PDK4. EIF4A3 and PDK4 were upregulated in OC cells. Silencing EIF4A3 obstructed cell proliferation and aerobic glycolysis, while the same was annulled by EIF4A3 overexpression. Mechanically, EIF4A3 could bind to PDK4 mRNA to stabilize its mRNA and upregulate its protein levels. PDK4 overexpression inverted the inhibitory role of silencing EIF4A3 in proliferation and aerobic glycolysis. Overall, our findings highlighted that EIF4A3 induced OC progression by stabilizing PDK4 mRNA.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10419146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Robotic arm use for upper limb rehabilitation after stroke: A systematic review and meta-analysis. 机械臂用于中风后上肢康复:一项系统回顾和荟萃分析。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12679

Bih-O Lee, Ita Daryanti Saragih, Sakti Oktaria Batubara

{"title":"Robotic arm use for upper limb rehabilitation after stroke: A systematic review and meta-analysis.","authors":"Bih-O Lee, Ita Daryanti Saragih, Sakti Oktaria Batubara","doi":"10.1002/kjm2.12679","DOIUrl":"https://doi.org/10.1002/kjm2.12679","url":null,"abstract":"Several studies have reported the effects of robotic arms on improving upper limb function in patients with stroke. However, previous studies have reported inconsistent findings that may lead to incorrect applications of robotic arm use. Six databases were searched for relevant randomized controlled trials. Meta-analyses were performed for upper limb performance measures, including subgroup analysis of pooled upper limb rehabilitation data such as stroke stage and intervention delivery dose. Furthermore, the Cochrane risk-of-bias tool for randomized trials version 2 (RoB 2) and sensitivity analysis were used to assess methodology and determine publication bias. The final analysis included 18 studies. Robotic arms improved upper limb and hand function in patients with stroke. Subgroup analysis revealed that robotic arm interventions lasting 30-60 min per session significantly improved upper limb function. However, no significant improvement was observed in shoulder and elbow or wrist and hand movements. This review may help develop applicable rehabilitation robots and collaboration between clinicians.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9441594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Obstructive jaundice caused by IgG4-related disease with multiorgan involvement. 多器官累及igg4相关疾病引起的梗阻性黄疸

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12668

Gwo-Kuang Yu, Shen-Nien Wang

{"title":"Obstructive jaundice caused by IgG4-related disease with multiorgan involvement.","authors":"Gwo-Kuang Yu, Shen-Nien Wang","doi":"10.1002/kjm2.12668","DOIUrl":"https://doi.org/10.1002/kjm2.12668","url":null,"abstract":"Immunoglobulin G4-related disease (IgG4-RD) is an immune-mediated disease with multiorgan involvement. The variable clinical manifestations and image findings of this disease often present a diagnostic dilemma for physicians. Herein, we describe a patient with IgG4-RD involving the kidney, retroperitoneum, pancreas, and biliary tracts, successfully treated with steroids. A 68-year-old man presented to our emergency room with a 2-week history of jaundice, tea-color urine, and poor appetite. He denied presence of fever, dyspnea, abdominal pain, or dysuria recently. Relevant laboratory data showed elevated liver enzymes and bilirubinemia (SGOT = 82 IU/L, SGPT = 174 IU/L, ALP = 196 IU/L, GGT = 340 IU/L, total bilirubin = 12 mg/dl, direct bilirubin = 7.51 mg/dl) with normal C-Reactive protein (CRP), blood cell counts and renal function. Abdominal computed tomography (CT) showed enhancing soft tissue lesions at bilateral renal pelvises (Figure 1A, arrow) and paraaortic regions (Figure 1B, arrow), diffuse swelling of the pancreas with a peripheral poorly enhanced halo (Figure C, short arrow) and dilatation of intra-hepatic and extra-hepatic bile ducts associated with enhancing wall thickenings (Figure 1 D, arrow). Urine cytology, performed twice, was negative for malignant cells. Ureterorenoscopy","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9490842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lavender essential oil accelerates lipopolysaccharide-induced chronic wound healing by inhibiting caspase-11-mediated macrophage pyroptosis. 薰衣草精油通过抑制caspase-11介导的巨噬细胞凋亡来加速脂多糖诱导的慢性伤口愈合。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12654

Xiang Ao, Huan Yan, Mei Huang, Wei Xing, Luo-Quan Ao, Xiao-Feng Wu, Cheng-Xiu Pu, Bao-Yue Zhang, Xiang Xu, Hua-Ping Liang, Wei Guo

{"title":"Lavender essential oil accelerates lipopolysaccharide-induced chronic wound healing by inhibiting caspase-11-mediated macrophage pyroptosis.","authors":"Xiang Ao, Huan Yan, Mei Huang, Wei Xing, Luo-Quan Ao, Xiao-Feng Wu, Cheng-Xiu Pu, Bao-Yue Zhang, Xiang Xu, Hua-Ping Liang, Wei Guo","doi":"10.1002/kjm2.12654","DOIUrl":"https://doi.org/10.1002/kjm2.12654","url":null,"abstract":"Chronic wounds seriously affect the quality of life of the elderly, obese people, and diabetic patients. The excessive inflammatory response is a key driver of delayed chronic wound healing. Although lavender essential oil (EO [lav]) has been proven to have anti-inflammatory and accelerate wound curative effects, the specific molecular mechanism involved is still ambiguous. The results showed that the wounds treated with lipopolysaccharide (LPS) not only had delayed healing, but also the expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the inflammatory mediator protein, high-mobility group box 1 protein (HMGB-1), in the wound tissues were significantly increased. However, treatment of LPS-induced chronic wounds with EO (lav) accelerated wound healing and decreased IL-1β and HMGB-1 expression levels. It was further found that LPS induced macrophage pyroptosis to produce IL-1β. After treatment with EO (lav), the expression level of macrophage pyroptosis marker Gasdermin D (GSDMD) and pyroptosis-related cytotoxic effects were significantly reduced. Immunofluorescence results also directly indicate that EO (lav) can protect macrophages from LPS-induced pyroptosis. Moreover, EO (lav) can down-regulate expression levels of IL-1β, GSDMD, and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) in the caspase-11-related pyroptotic signaling pathway. This study demonstrates that EO (lav) can reduce proinflammatory factor production and ameliorate inflammatory response by inhibiting macrophage pyroptosis, which accelerates LPS-induced chronic wound healing.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9435295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF-κB signaling axis to alleviate atopic dermatitis. 苦参碱通过抑制Hsp90/NF-κB信号轴调节Th1/Th2炎症反应，减轻特应性皮炎。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12655

Pan Huang, Fan Hu, Zhi-Bo Yang, Yi Pan, Rong Zhou, Yi-Ning Yan, Hai-Zhen Wang, Chang Wang

{"title":"Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF-κB signaling axis to alleviate atopic dermatitis.","authors":"Pan Huang, Fan Hu, Zhi-Bo Yang, Yi Pan, Rong Zhou, Yi-Ning Yan, Hai-Zhen Wang, Chang Wang","doi":"10.1002/kjm2.12655","DOIUrl":"https://doi.org/10.1002/kjm2.12655","url":null,"abstract":"Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real-time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme-linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T-helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin-6) secretions by repressing the Hsp90/NF-κB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4+ T cells when co-cultured with inflamed HaCaT cells. Matrine can regulate the Th1/Th2 inflammatory response by inhibiting the Hsp90/NF-κB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9435297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Impact of linked color imaging and blue laser imaging on the diagnosis of esophageal squamous cell carcinoma in iodine unstained areas. 彩色和蓝色激光联合成像对碘未染色区食管鳞状细胞癌诊断的影响。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-05-01 DOI: 10.1002/kjm2.12660

Masato Tsunoda, Yoshimasa Miura, Hiroyuki Osawa, Manabu Nagayama, Yuka Kagaya, Yohei Funayama, Takuma Kobayashi, Mami Togashi, Hiroki Hayashi, Yuji Hiraoka, Yoshie Nomoto, Chihiro Iwashita, Yuji Ino, Haruo Takahashi, Hisashi Fukuda, Alan Kawarai Lefor, Hironori Yamamoto

{"title":"Impact of linked color imaging and blue laser imaging on the diagnosis of esophageal squamous cell carcinoma in iodine unstained areas.","authors":"Masato Tsunoda, Yoshimasa Miura, Hiroyuki Osawa, Manabu Nagayama, Yuka Kagaya, Yohei Funayama, Takuma Kobayashi, Mami Togashi, Hiroki Hayashi, Yuji Hiraoka, Yoshie Nomoto, Chihiro Iwashita, Yuji Ino, Haruo Takahashi, Hisashi Fukuda, Alan Kawarai Lefor, Hironori Yamamoto","doi":"10.1002/kjm2.12660","DOIUrl":"https://doi.org/10.1002/kjm2.12660","url":null,"abstract":"The pink color sign in iodine unstained areas is useful to differentiate esophageal squamous cell carcinoma (ESCC) from other lesions. However, some ESCCs have obscure color findings which affect the ability of endoscopists to differentiate these lesions and determine the resection line. Using white light imaging (WLI), linked color imaging (LCI) and blue laser imaging (BLI), 40 early ESCCs were retrospectively evaluated using images before and after iodine staining. Visibility scores for ESCC by expert and non-expert endoscopists were compared using these three modalities and color differences measured for malignant lesions and surrounding mucosa. BLI had the highest score and color difference without iodine staining. Each determination with iodine was much higher than without iodine regardless of the modality. With iodine, ESCC mainly appeared pink, purple and green using WLI, LCI and BLI, respectively and visibility scores determined by non-experts and experts were significantly higher for LCI (both p < 0.001) and BLI (p = 0.018 and p < 0.001) than for WLI. The score with LCI was significantly higher than with BLI among non-experts (p = 0.035). With iodine, the color difference using LCI was twice that with WLI and one with BLI was significantly larger than with WLI (p < 0.001). These greater tendencies were found regardless of location, depth of cancer or intensity of pink color using WLI. In conclusion, areas of ESCC unstained by iodine were easily recognized using LCI and BLI. Visibility of these lesions is excellent even by non-expert endoscopists, suggesting that this method is useful to diagnose ESCC and determine the resection line.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9804410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1