Kaohsiung Journal of Medical Sciences最新文献

Retraction: Hong Liu, Shi-Ying Ren, Yan Qu, Cui Liu, Yi Zhang, Xiang Qing Li, Hong Ma. MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk. The Kaohsiung Journal of Medical Sciences, Volume 36, Issue 4 Apr 2020. Pages 265-273. https://doi.org/10.1002/kjm2.12180. 撤回：Hong Liu, Shi-Ying Ren, Yan Qu, Cui Liu, Yi Zhang, Xiang Qing Li, Hong Ma.MiR-194-5p通过靶向Crk抑制肾母细胞瘤细胞的转移和EMT高雄医学志》第36卷第4期，2020年4月。https://doi.org/10.1002/kjm2.12180.

IF 2.7 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2024-10-01 Epub Date: 2023-06-28 DOI: 10.1002/kjm2.12727

引用次数: 0

Analysis of macular choroidal thickness in normal Taiwanese eyes by enhanced depth imaging optical coherence tomography. 通过增强深度成像光学相干断层扫描分析正常台湾人眼睛的黄斑脉络膜厚度。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-12-01 Epub Date: 2023-09-01 DOI: 10.1002/kjm2.12750

Li-Wen Chiu, Yo-Chen Chang, Kuo-Jen Chen, Kai-Chun Cheng

引用次数: 0

Bloodletting acupuncture on venules between BL60 and BL61 rapidly relieving a 4-month episode of low back pain. 针刺 BL60 和 BL61 之间的静脉放血，可迅速缓解为期 4 个月的腰痛。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-12-01 Epub Date: 2023-09-06 DOI: 10.1002/kjm2.12755

Shih-Yun Hsu, Frank Art Lin, Chung-Jen Chen

引用次数: 0

Mechanism of DYRK1a in myocardial ischemia-reperfusion injury by regulating ferroptosis of cardiomyocytes. DYRK1a 通过调节心肌细胞的铁凋亡在心肌缺血再灌注损伤中的作用机制

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-12-01 Epub Date: 2023-09-13 DOI: 10.1002/kjm2.12753

Jing Wang, Rui-Ming Xu, Qiu-Mei Cao, Bing-Chen Ma, Hao Zhang, Hua-Peng Hao

{"title":"Mechanism of DYRK1a in myocardial ischemia-reperfusion injury by regulating ferroptosis of cardiomyocytes.","authors":"Jing Wang, Rui-Ming Xu, Qiu-Mei Cao, Bing-Chen Ma, Hao Zhang, Hua-Peng Hao","doi":"10.1002/kjm2.12753","DOIUrl":"10.1002/kjm2.12753","url":null,"abstract":"This study aimed to explore the role and mechanism of DYRK1a regulating ferroptosis of cardiomyocytes during myocardial ischemia-reperfusion injury (MIRI). H9c2 cells treated with oxygen-glucose deprivation/reoxygenation (OGD/R) were used as MIRI cell models and transfected with sh-DYRK1a or/and erastin. Cell viability, apoptosis, and DYRK1a mRNA/protein expression were measured accordingly. The levels of reactive oxygen species (ROS), iron, malondialdehyde (MDA), and glutathione (GSH) were determined. The expression of ferroptosis-related proteins (GPX4, SLC7A11, ACSL4, and TFR1) was detected using western blotting. The MIRI rat model was established to explore the possible role of DYRK1a suppression in cell injury and ferroptosis. OGD/R cells showed elevated mRNA and protein expression for DYRK1a. OGD/R cells transfected with sh-DYRK1a showed elevated cell viability, GSH content, increased GPX4 and SLC7A11 expression, suppressed iron content, MDA, ROS, ACSL4, and TFR1 expression, and reduced apoptosis rate, whereas co-transfection of sh-DYRK1a with erastin reversed the attenuation of sh-DYRK1a on MIRI. The suppressive effect of sh-DYRK1a on MI/R injury was confirmed in an MIRI rat model. DYRK1a mediates ferroptosis of cardiomyocytes to deteriorate MIRI progression.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ_0084188 promotes colorectal cancer progression by sponging miR-654-3p and regulating kruppel-like factor 12. Circ_0084188通过调控miR-654-3p和kruppel-like factor 12促进结直肠癌进展。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-11-01 Epub Date: 2023-09-12 DOI: 10.1002/kjm2.12749

Cui-Cui Wu, Bai-Chun Hou, Yu-Han Yang, Xue-Feng Li, Hong-Chao Ma, Bin-Xian Li

{"title":"Circ_0084188 promotes colorectal cancer progression by sponging miR-654-3p and regulating kruppel-like factor 12.","authors":"Cui-Cui Wu, Bai-Chun Hou, Yu-Han Yang, Xue-Feng Li, Hong-Chao Ma, Bin-Xian Li","doi":"10.1002/kjm2.12749","DOIUrl":"10.1002/kjm2.12749","url":null,"abstract":"To investigate the biological role and mechanism of circ_0084188 in colorectal cancer (CRC). Real-time quantitative polymerase chain reaction and western blot assay were used to detect RNA levels and protein levels in CRC cell lines (HCT116 and SW480), respectively. Cell proliferation was evaluated by Cell Counting Kit-8 assay, 5-ethynyl-2'-deoxyuridine assay, and colony formation assays. Cell apoptosis was determined using flow cytometry. Cell migration and invasion were measured by transwell assay. Sphere formation efficiency was determined by sphere formation assay. The interaction between microRNA-654-3p (miR-654-3p) and circ_0084188 or Kruppel-like factor 12 (KLF12) was confirmed by a dual-luciferase reporter, RNA immunoprecipitation and RNA pull-down assays. Xenograft in CRC mice model was utilized for exploring the role of circ_0084188 in vivo.Circ_0084188 was overexpressed in CRC tissues and cells. Circ_0084188 silencing suppressed cell proliferation, migration, invasion, and stemness and induced apoptosis in CRC cells. Circ_0084188 acted as a sponge for miR-654-3p, and circ_0084188 regulated CRC cell behaviors via sponging miR-654-3p. Moreover, KLF12 was a target of miR-654-3p, and miR-654-3p overexpression inhibited the malignant behaviors of CRC cells by downregulating KLF12. Mechanically, circ_0084188 sponged miR-654-3p to regulate KLF12 expression in CRC cells. In addition, circ_0084188 downregulation inhibited tumor growth in vivo.Circ_0084188 knockdown might repress CRC progression partially via regulating the miR-654-3p/KLF12 axis, providing a novel insight into the pathogenesis of CRC.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXA1 prolongs S phase and promotes cancer progression in non-small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways. FOXA1通过上调CDC5L和激活ERK1/2和JAK2通路，延长非小细胞肺癌的S期并促进癌症进展。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-11-01 Epub Date: 2023-09-02 DOI: 10.1002/kjm2.12737

Gang Chen, Rui-Shi Wei, Jie Ma, Xin-Hua Li, Li Feng, Jian-Rong Yu

{"title":"FOXA1 prolongs S phase and promotes cancer progression in non-small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways.","authors":"Gang Chen, Rui-Shi Wei, Jie Ma, Xin-Hua Li, Li Feng, Jian-Rong Yu","doi":"10.1002/kjm2.12737","DOIUrl":"10.1002/kjm2.12737","url":null,"abstract":"Non-small cell lung cancer (NSCLC) causes high mortality worldwide; however, its molecular pathways have not been fully investigated. The relationship between FOXA1 and CDC5L as well as their roles in NSCLC have not been comprehensively studied. Clinical tissues were collected from 78 NSCLC patients for clinical studies. The BEAS-2B human normal lung epithelial cell line and the A549, Calu-3, H526 and H2170 human NSCLC cell lines were used for in vitro studies. sh-FOXA1 and oe-CDC5L constructs were used to generate knockdown and overexpression models, respectively. The CCK-8 assay was used to analyze cell viability. The cell cycle and apoptosis were evaluated by flow cytometry analysis. The relationship between FOXA1 and CDC5L was demonstrated using dual-luciferase and ChIP assays. Gene levels were examined via immunohistochemistry, qRT-PCR and western blot analysis. FOXA1 levels were increased in NSCLC clinical tissues and cell lines. Depletion of FOXA1 increased the apoptosis rate and increased the proportion of cells in G2/M phase. In addition, we demonstrated that FOXA1 was directly bound to the promoter of CDC5L and that depletion of FOXA1 inhibited CDC5L expression. Overexpression of CDC5L induced ERK1/2 phosphorylation, induced JAK2 phosphorylation, inhibited cell apoptosis, prolonged S phase, and significantly reversed the effects of FOXA1 knockdown on the progression of NSCLC. The present study demonstrated that FOXA1 prolongs S phase and promotes NSCLC progression through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10511545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neferine inhibits the development of lung cancer cells by downregulating TGF-β to regulate MST1/ROS-induced pyroptosis. 莲子碱通过下调TGF-β调控MST1/ ros诱导的肺癌细胞焦亡，从而抑制肺癌细胞的发育。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-11-01 Epub Date: 2023-09-12 DOI: 10.1002/kjm2.12752

Peng-Cheng Zhong, Zhi-Wen Liu, Qi-Chang Xing, Jia Chen, Rui-Pei Yang

{"title":"Neferine inhibits the development of lung cancer cells by downregulating TGF-β to regulate MST1/ROS-induced pyroptosis.","authors":"Peng-Cheng Zhong, Zhi-Wen Liu, Qi-Chang Xing, Jia Chen, Rui-Pei Yang","doi":"10.1002/kjm2.12752","DOIUrl":"10.1002/kjm2.12752","url":null,"abstract":"Non-small cell lung cancer (NSCLC) accounts for ~85% of all lung cancer cases. Neferine is used as a traditional Chinese medicine with many pharmacological effects, including antitumor properties; however, it has not been reported whether neferine plays an anticancer role by causing pyroptosis in NSCLC cells. We used two typical lung cancer cell lines, A549 and H1299, and 42 lung cancer tissue samples to investigate the regulatory effects of neferine on TGF-β and MST1. We also treated lung cancer cells with different concentrations of neferine to study its effects on lung cancer cell survival, migration, invasion, and epithelial-mesenchymal transition (EMT) as well as on pyroptosis. Lentivirus-mediated gain-of-function studies of TGF-β and MST1 were applied to validate the roles of TGF-β and MST1 in lung cancer. Next, we used murine transplanted tumor models to evaluate the effect of neferine treatment on the metastatic capacity of lung cancer tissues. With increasing neferine concentration, the viability, migration, invasion, and EMT capacity of A549 and H1299 cells decreased, whereas pyroptosis increased. Neferine repressed TGF-β expression to modulate the induction of reactive oxygen species (ROS) by MST1. Overexpression of TGF-β in either in vitro or mouse-transplanted A549 cells restored the inhibitory effect of neferine on tumor development. Overexpression of MST1 clearly enhanced pyroptosis. Neferine contributed to pyroptosis by regulating MST1 expression through downregulation of TGF-β to induce ROS formation. Therefore, our study shows that neferine can serve as an adjuvant therapy for NSCLC patients.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10213118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Choroid plexus papilloma at the root entry zone causing hemifacial spasm. 脉络膜丛乳头状瘤在根入口区引起面肌痉挛。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-11-01 Epub Date: 2023-08-24 DOI: 10.1002/kjm2.12747

Yi-Yun Chen, Ming-Tsung Chuang, Shih-Huang Tai, E-Jian Lee

引用次数: 0

Unawareness of hepatitis B infection and lack of surveillance are associated with severity of hepatocellular carcinoma. 对乙型肝炎感染的不了解和缺乏监测与肝细胞癌的严重程度有关。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-11-01 Epub Date: 2023-09-02 DOI: 10.1002/kjm2.12744

Kuan-I Lee, Po-Cheng Liang, Po-Yau Hsu, Tyng-Yuan Jang, Yu-Ju Wei, Ching-I Huang, Ming-Yen Hsieh, Zu-Yau Lin, Ming-Lun Yeh, Chung-Feng Huang, Jee-Fu Huang, Chia-Yen Dai, Wan-Long Chuang, Ming-Lung Yu

{"title":"Unawareness of hepatitis B infection and lack of surveillance are associated with severity of hepatocellular carcinoma.","authors":"Kuan-I Lee, Po-Cheng Liang, Po-Yau Hsu, Tyng-Yuan Jang, Yu-Ju Wei, Ching-I Huang, Ming-Yen Hsieh, Zu-Yau Lin, Ming-Lun Yeh, Chung-Feng Huang, Jee-Fu Huang, Chia-Yen Dai, Wan-Long Chuang, Ming-Lung Yu","doi":"10.1002/kjm2.12744","DOIUrl":"10.1002/kjm2.12744","url":null,"abstract":"Unawareness of hepatitis B virus (HBV) infection and lack of surveillance may serve as major barriers to HBV control and contributors to severe hepatocellular carcinoma (HCC) at presentation. This study evaluated the risk of HBV unawareness and its relationship with HCC severity. This retrospective study was conducted in a tertiary hospital in Taiwan. Patients with HBV-related HCC diagnosed from 2011 to 2021 were enrolled. The demographic, clinical, and HCC characteristics were collected and compared between patients with HBV unawareness and awareness with and without surveillance. Of 501 HBV-related HCC patients enrolled, 105 (21%) patients were unaware of HBV infection at the time of HCC diagnosis. Patients with HBV unawareness were significantly younger and had poorer liver function than those with HBV awareness. Patients with HBV unawareness also had a significantly higher rate of detectable HBV DNA and an advanced stage of HCC. Ninety-one (23%) of the HBV-aware patients did not receive regular surveillance. Patients with HBV unawareness and awareness without surveillance shared similar clinical characteristics with more severe HCC status. Further regression analysis demonstrated that HBV awareness with periodic surveillance was associated with early stage HCC. Meanwhile, we observed that there was no change in the proportion of HBV awareness over the past 10 years. Patients with surveillance also had better HCC survival than patients without surveillance or unawareness. HBV unawareness and lack of regular surveillance correlated with advanced HCC at presentation. Efforts to improve HBV education, disease awareness, and HCC surveillance are needed.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10194666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silenced LASP1 interacts with DNMT1 to promote TJP2 expression and attenuate articular cartilage injury in mice by suppressing TJP2 methylation. 沉默的LASP1与DNMT1相互作用，通过抑制TJP2甲基化，促进TJP2表达，减轻小鼠关节软骨损伤。

IF 3.3 4区医学

Kaohsiung Journal of Medical Sciences Pub Date : 2023-11-01 Epub Date: 2023-08-14 DOI: 10.1002/kjm2.12738

Lian Ren, Shi-Gao Cheng, Peng-Cheng Kang, Teng-Fei Li, Xun Li, Jiong-Zhe Xiao, Dong Jiang

{"title":"Silenced LASP1 interacts with DNMT1 to promote TJP2 expression and attenuate articular cartilage injury in mice by suppressing TJP2 methylation.","authors":"Lian Ren, Shi-Gao Cheng, Peng-Cheng Kang, Teng-Fei Li, Xun Li, Jiong-Zhe Xiao, Dong Jiang","doi":"10.1002/kjm2.12738","DOIUrl":"10.1002/kjm2.12738","url":null,"abstract":"To investigate the regulatory mechanisms and effects of LIM and SH3 protein 1 (LASP1) on osteoarthritis (OA). IL-1β was used to induce OA in cell models. Viability and apoptosis of chondrocytes were assessed. The expressions of tumor necrsis factor-α (TNF-α) and IL-6 were measured by ELISA kit, and Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were performed to test the expression of related proteins. The STRING database was used to predict the relationship between LASP1 and DNA methyltransferase 1 (DNMT1). The tight junction protein 2 (TJP2) and Gene Expression Omnibus data were analyzed for differential OA genes. Methylation-specific PCR detected methylation of the TJP2 promoter region, and chromatin immunoprecipitation detected the enrichment of DNMT1 in the TJP2 promoter region. Safranin O-Fast Green staining and hematoxylin and eosin staining were used to determine the OARSI score and evaluate the pathological conditions of the joint tissues. LASP1 was highly expressed in IL-1β-induced cell models. Silencing of LASP1 promoted chondrocyte proliferation and expression of Collagen II and Aggrecan and inhibited chondrocyte apoptosis, inflammatory factors, and matrix metalloprotein expression. TJP2 is weakly expressed in OA models, and LASP1 promotes methylation of the TJP2 promoter region by interacting with DNMT1. Silencing of LASP1 attenuated IL-1β-induced chondrocyte degeneration by promoting TJP2 expression. Similarly, silencing LASP1 promotes TJP2 expression to alleviate articular cartilage injury in mice with OA. Silencing of LASP1 inhibited the methylation of the TJP2 promoter region by interacting with DNMT1, thereby alleviating articular cartilage damage in OA mice.","PeriodicalId":49946,"journal":{"name":"Kaohsiung Journal of Medical Sciences","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9993773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0