Laboratoriumsmedizin-Journal of Laboratory Medicine最新文献_第4页

Medical laboratory in autoimmunity 2017 自身免疫医学实验室2017

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-08-28 DOI: 10.1515/labmed-2017-0077

U. Nydegger

{"title":"Medical laboratory in autoimmunity 2017","authors":"U. Nydegger","doi":"10.1515/labmed-2017-0077","DOIUrl":"https://doi.org/10.1515/labmed-2017-0077","url":null,"abstract":"Abstract With big data algorithms and artificial intelligence (AI) at stake the optimal assembly of the most appropriate lab assays selected to diagnose, treat and follow up patients suffering from well-delineated disease may get lost. The physician ordering a lab test, instead of asking for a good composition of screening tests is tempted to order a large number of assays, including genome sequencing hoping to find the diagnostic evidence for his/her patient at once. Four major specialities of medical laboratory assays, i.e. clinical chemistry, hematology, immunology and microbiology are embraced by genome sequencing techniques and have attained the degree of robotics, facilitating assays to such a degree, that the prescriber is free of concern as to how costly/complicated an investigation might become. Diagnostics with autoimmune diseases is not an exemption and autoantibody screening using multiplex assays or therapeutic drug monitoring to adjust treatments of inflammatory/autoimmune diseases is bound to become more and more informative even more so as the pharmacodynamics of modern pharmaceutical agents are explored. As the most appropriate therapeutical agents to monitor in the lab, biological response modifiers, immunosuppressants and monoclonal antibodies are at the forefront and we need to explore their efficacy and side effect profiles not only using phase III clinical studies but also by using postmarketing surveillance. Behind the profiles provided by big data and artificial intelligence, the therapeutically-induced regained immune balance can thus be traced to the single best lab assay. The next decade promises a series of new assays, e.g. inflammasome profiles, lymphocyte markers by fluorescence activated cell sorters as well as single cell secretome analysis.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"6 1-2 1","pages":"173 - 182"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79181461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyses of exhaled breath condensate cytokines for identification of lung cancer 呼气凝析液细胞因子在肺癌鉴别中的分析

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-08-28 DOI: 10.1515/labmed-2017-0054

C. Gessner, P. Ruschpler, S. Fricke, A. Gillissen, Gerhard Hoheisel, J. Lehmann, U. Sack

{"title":"Analyses of exhaled breath condensate cytokines for identification of lung cancer","authors":"C. Gessner, P. Ruschpler, S. Fricke, A. Gillissen, Gerhard Hoheisel, J. Lehmann, U. Sack","doi":"10.1515/labmed-2017-0054","DOIUrl":"https://doi.org/10.1515/labmed-2017-0054","url":null,"abstract":"Abstract Early non-invasive detection of lung cancer is a precondition for enabling better prognosis supported by new innovative therapy regimes. The aim of our study was to evaluate angiogenic and inflammatory proteins in exhaled breath condensate (EBC) as markers for lung cancer. Our report presents a diagnostic study of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and tumor necrosis factor-α (TNF-α) in EBC of 300 individuals, 84 patients with lung cancer, 111 patients with stable chronic obstructive pulmonary disease (COPD), and in 105 healthy controls. Detection of VEGF and bFGF in EBC was applicable to discriminate cancer patients from COPD patients as well as from healthy volunteers. Especially VEGF seems to be suitable to discriminate between non-small cell lung cancer (NSCLC) patients and control groups with highest VEGF values in EBC of patients with progressive NSCLC. The concentration of angiogenic factors correlated with disease progression as well as higher tumor stage. This study supports cytokine analysis in EBC as a suitable noninvasive diagnostic screening method for lung cancer detection and monitoring.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"32 1","pages":"187 - 194"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83112604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The values of mean platelet volume and the mean platelet volume/platelet ratio for predicting deep venous thrombosis in Behçet’s disease 平均血小板体积和平均血小板体积/血小板比值对behaperet病深静脉血栓形成的预测价值

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2017-0068

F. Erden, Hatice Karagoz, A. Avcı, D. Avcı, A. Çetinkaya, A. Erden

{"title":"The values of mean platelet volume and the mean platelet volume/platelet ratio for predicting deep venous thrombosis in Behçet’s disease","authors":"F. Erden, Hatice Karagoz, A. Avcı, D. Avcı, A. Çetinkaya, A. Erden","doi":"10.1515/labmed-2017-0068","DOIUrl":"https://doi.org/10.1515/labmed-2017-0068","url":null,"abstract":"Abstract Background: Behçet’s disease (BD) is a systemic vasculitis of all types of vessels of both the venous and arterial sites. Mean platelet volume (MPV) is the most commonly used measure of platelet (Plt) size and is also a potential marker of Plt activity. In this study, we wanted to evaluate the MPV value and the MPV/Plt ratio in patients with deep venous thrombosis (DVT) in BD. Methods: Overall, 228 patients – 49 BD patients with a thrombotic complication and 179 BD patients without a thrombotic complication (as the control group) – were included the study. Results: Of the 49 patients with thrombosis, there was a very significant difference between the genders: 41 (83.6%) were males while only eight (16.4%) were females (p<0.0001). There was a statistically significant difference among the patients with and without thrombosis according to the median MPV value and the MPV/Plt ratio (p<0.0001). Conclusions: Increased MPV can predict the risk of DVT development as well as the male sex among BD patients. The MPV/Plt ratio may also be used for predicting DVT.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"29 1","pages":"153 - 157"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74904918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The use of LC tandem mass spectrometry as part of a workflow for the screening and identification of hemoglobin variants. Characterization of Hb Ullevaal as an example 使用LC串联质谱作为筛选和鉴定血红蛋白变异的工作流程的一部分。以Hb Ullevaal的表征为例

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2017-0036

Matthias Weber, Julia J. M. Eekels

{"title":"The use of LC tandem mass spectrometry as part of a workflow for the screening and identification of hemoglobin variants. Characterization of Hb Ullevaal as an example","authors":"Matthias Weber, Julia J. M. Eekels","doi":"10.1515/labmed-2017-0036","DOIUrl":"https://doi.org/10.1515/labmed-2017-0036","url":null,"abstract":"Abstract Background: About 2/3 of the hemoglobin (Hb) variants do not show a charge difference to the wildtype entity but most of them differ in hydrophobicity. In addition to cation exchange chromatography, globin differentiation by liquid chromatography-tandem mass spectrometry (MS) was introduced. Hb Ullevaal was chosen as one example to demonstrate the performance of the approach. Methods: Screening for Hb variants was performed using cation exchange HPLC. For globin separation reversed phase-LC/MS was performed. Tryptic digests of variants were separated on RP-HPLC with or without CID-fragmentation and database search for identification of mutation bearing fragments. Sequencing of the β-globin gene has been performed. Results: HbS, HbC, HbE, Hb South Florida and Hb Ullevaal show typical and distinct patterns in the globin LC/MS according to the theoretical protein data. The tryptic digest of Hb Ullevaal resulted in the identification of the respective mutated peptide βT9, which was confirmed by genetic sequencing. Conclusions: By the application of globin-LC/MS two more dimensions for the Hb identification are added, hydropathicity and protein mass. With this workflow as screening procedure for Hb variants it is expected to be able to detect and identify the majority of variants with the exception of highly unstable variants, which cannot be determined in the peripheral blood at all. A negative result makes the presence of a significant Hb variant in the peripheral blood improbable.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"30 2 1","pages":"137 - 145"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80399734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bisalbuminämie: Normvariante oder pathologischer Befund? 检验结果

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2017-0055

M. Steiner, J. Lüdemann

引用次数: 0

Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy 无并发症妊娠期间瘦素和脂联素浓度的纵向变化

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2017-0052

Marina Pijanović, A. Stefanović, M. Miljkovic, Snežana Marić-Krejović, S. Spasić

{"title":"Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy","authors":"Marina Pijanović, A. Stefanović, M. Miljkovic, Snežana Marić-Krejović, S. Spasić","doi":"10.1515/labmed-2017-0052","DOIUrl":"https://doi.org/10.1515/labmed-2017-0052","url":null,"abstract":"Abstract Background: Leptin and adiponectin play an important role during normal gestation; they are implicated in energy metabolism, glucose utilization and inflammation. Osteocalcin is released into circulation during bone formation; it also affects glucose metabolism by regulating insulin secretion and sensitivity, possibly mediated by adiponectin. The aim of this study was to explore the longitudinal changes of leptin and adiponectin in pregnancy, and their associations with lipid profile, insulin and bone formation parameters in late pregnancy. Methods: Leptin, adiponectin, lipid status parameters, C-reactive protein (CRP), insulin, 25-hydroxyvitamin D, osteocalcin and procollagen type 1 aminoterminal propeptide (P1NP) were measured in the sera of 38 healthy pregnant women. The samples were obtained in the 1st, 2nd, early and late 3rd trimester, and post-partum. Results: Leptin was significantly increased in the 3rd trimester. The decrease of adiponectin was significant only in postpartum. Osteocalcin and P1NP increased in the late 3rd trimester and postpartum. Leptin was significantly positively correlated with body mass index (BMI), uric acid, insulin, osteocalcin, P1NP and CRP in the 3rd trimester; adiponectin was positively correlated with high-density lipoprotein (HDL) cholesterol, and negatively with BMI, glucose, osteocalcin, triglycerides and insulin. Multiple regression analysis showed that only HDL is independently associated with adiponectin. Conclusions: The results of our study suggest complex interactions of leptin and adiponectin with glucose, lipid and bone metabolism during pregnancy. Adiponectin might be part of the protective systems that counterbalance a transient proatherogenic state observed in pregnancy mainly by improving the HDL levels. The exact mechanisms and potential implications in pathological states of pregnancy remain unexplained and require further investigation.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"1 1","pages":"129 - 136"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85519476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylenetetrahydrofolate reductase gene polymorphism and clinical importance in epilepsy patients using valproic acid, carbamazepine and levetiracetam 丙戊酸、卡马西平和左乙拉西坦治疗癫痫患者亚甲基四氢叶酸还原酶基因多态性及其临床意义

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2016-0059

A. Aydın, A. Varoğlu

{"title":"Methylenetetrahydrofolate reductase gene polymorphism and clinical importance in epilepsy patients using valproic acid, carbamazepine and levetiracetam","authors":"A. Aydın, A. Varoğlu","doi":"10.1515/labmed-2016-0059","DOIUrl":"https://doi.org/10.1515/labmed-2016-0059","url":null,"abstract":"Abstract Background: We aim to determine the relationships among vitamin B12, folic acid, homocysteine (Hcy), and methylenetetrahydrofolatereductase (MTHFR) C677T polymorphism, as well as the clinical importance of these relationships, in patients using valproic acid (VPA), carbamazepine (CBZ), and levetiracetam (LEV) as monotherapy and polytherapy. Methods: We enrolled 37 patients on VPA, 30 on CBZ, 31 on LEV, 30 on multidrug therapy, and 60 control subjects. We compared the levels of vitamin B12, folic acid, Hcy and polymorphism. Results: Vitamin B12 was low in patients on CBZ (p=0.02) and in combined CBZ and VPA (p=0.02). B12 was low in combined CBZ and VPA (p=0.05). In patients without polymorphism, Hcy was high on VPA (p=0.02), and folic acid was the low on CBZ (0.005). In patients with polymorphism, vitamin B12 was low on CBZ (p=0.02), and folic acid was low on VPA (p=0.04). Vitamin B12 was low in combined CBZ and VPA (p=0.05). Conclusions: Vitamin B12 therapy is necessary on CBZ and on combined CBZ and VPA. VPA should not be used in the presence of other thrombophilic risk factors because of hyperhomocysteinemia. Polytherapy does not increase hyperhomocysteinemia risk in comparison to monotherapy. Vitamin B12, folic acid, Hcy do not effect on seizure frequency.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"29 1","pages":"147 - 151"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84928215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Investigation of the relation of maternal serum endocan levels to preeclampsia presence and severity 母体血清内啡肽水平与子痫前期存在及严重程度关系的研究

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2017-0002

Serdar Gülşen, Y. Çekmez, I. Ulu, Şebnem Garip, F. Aksoy, S. B. Türkmen, G. Gök, G. Kıran

引用次数: 1

Diagnostic values of proenkephalin and S100B protein in traumatic brain injury 脑前啡肽和S100B蛋白在颅脑外伤中的诊断价值

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-07-26 DOI: 10.1515/labmed-2016-0045

Anil Yalcin, A. Baydın, Ö. K. Tunçel, A. K. Erenler, C. Çokluk, M. Güzel, L. Tomak

{"title":"Diagnostic values of proenkephalin and S100B protein in traumatic brain injury","authors":"Anil Yalcin, A. Baydın, Ö. K. Tunçel, A. K. Erenler, C. Çokluk, M. Güzel, L. Tomak","doi":"10.1515/labmed-2016-0045","DOIUrl":"https://doi.org/10.1515/labmed-2016-0045","url":null,"abstract":"Abstract Background: The primary aim of this study was to investigate the diagnostic values of serum S100 calcium-binding protein B (S100B) and proenkephalin (P-ENK) levels in brain damage caused by traumatic brain injury (TBI). Methods: We prospectively collected serum blood samples of 58 adult patients admitted to our emergency department due to TBI. Serum S100B and P-ENK levels were measured and compared according to clinical findings and outcomes of the patients. Results: When patients with brain injury were compared to controls, statistical significance was determined in both S100B and P-ENK levels. According to the receiver operating characteristic (ROC) analysis, cut-off values for serum S100B and P-ENK levels for the differential diagnosis of patients with and without brain damage were found to be 785.944 ng/mL and 2.445 ng/mL, respectively. There was a statistical significance in both S100B and P-ENK levels when patients who were discharged and those who died were compared. Conclusions: Serum S100B and P-ENK levels are found to be elevated in patients with TBI when compared to controls. Additionally, serum levels of both markers are found to be elevated in patients with multiple lesions when compared to patients with a single lesion. Serum S100B and P-ENK levels may also be used as predictors of mortality in patients with TBI.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"17 1","pages":"123 - 128"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82905845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluating regional variability in the use of the most commonly requested laboratory tests in primary care in Spain: data from the multi-center national scale REDCONLAB initiative 评估西班牙初级保健中使用最常用实验室检测的区域差异:来自多中心国家规模REDCONLAB倡议的数据

Laboratoriumsmedizin-Journal of Laboratory Medicine Pub Date : 2017-04-25 DOI: 10.1515/labmed-2016-0077

M. Salinas, M. López-Garrigós, E. Flores, C. Leiva-Salinas

{"title":"Evaluating regional variability in the use of the most commonly requested laboratory tests in primary care in Spain: data from the multi-center national scale REDCONLAB initiative","authors":"M. Salinas, M. López-Garrigós, E. Flores, C. Leiva-Salinas","doi":"10.1515/labmed-2016-0077","DOIUrl":"https://doi.org/10.1515/labmed-2016-0077","url":null,"abstract":"Abstract Background: The aim was to study the regional variability in the request of the ten most frequently ordered laboratory tests in primary care in Spain. Methods: Spain is divided into autonomous communities (AACC), first level health care divisions. Every AACC is divided into health departments (HDs). A laboratory attends the needs of every HD inhabitant. Laboratories from different HDs participated in the study. They reported the request of the ten most commonly requested laboratory tests in primary care during the year 2014 according to prior evidence: alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, creatinine, γ-glutamyl transpeptidase (GGT), glucose, HDL-cholesterol, triglycerides, uric acid and urinalysis. Test-utilization rates were calculated as tests per 1000 inhabitants. Laboratories were grouped in the different AACC and the results for each region were compared using the coefficient of quartile dispersion (CQD), calculated using the first (Q1) and third (Q3) quartiles for each data set, as follows: (Q3−Q1)/(Q3+Q1). Results: One hundred and ten laboratories participated, corresponding to 27,798,262 inhabitants (59.8% Spanish population) from 15 AACC. 82,710,869 tests were requested. AST, GGT and uric acid showed the greatest variation. Conclusions: There were significant regional differences in how the most common laboratory tests were ordered in Spain.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"603 1","pages":"104 - 99"},"PeriodicalIF":0.0,"publicationDate":"2017-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83785310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0