Ernesto Larios‐Soriano, F. Díaz, A. Re-Araujo, L. López, Laura L. López-Galindo, C. True, C. A. Álvarez, M. Galaviz
{"title":"Influence of temperature on respiratory metabolism during early development of Totoaba macdonaldi","authors":"Ernesto Larios‐Soriano, F. Díaz, A. Re-Araujo, L. López, Laura L. López-Galindo, C. True, C. A. Álvarez, M. Galaviz","doi":"10.3856/vol51-issue1-fulltext-2952","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2952","url":null,"abstract":"Environmental temperature can act as a positive or negative modulator of the physiology and metabolism of poikilothermic organisms. As a general rule, larvae and juveniles are more sensitive to temperature stress than adults, which represents a key factor that partly determines their development and growth in aquaculture. Therefore, this study analyzed the effect of exposure to temperatures of 21, 24, and 27°C on the respiratory metabolism (RM) of Totoaba macdonaldi in different developmental stages. For this purpose, eggs, larvae with 4, 6, 8, 14, and 22 days post-hatch (dph), and juveniles of 25 dph were exposed to the experimental temperatures for 5 h. After the exposure time, oxygen consumption measurements were performed. The results clearly show that temperature (21 to 27°C) has the greatest effect on RM in eggs and larvae at 4 and 22 dph (3.1 ± 0.3 to 4.3 ± 0.3 μmol O2 h-1 egg-1, 2.9 ± 0.3 to 10.5 ± 1.2 μmol O2 h-1 larvae-1 and 102.0 ± 6.4 to 189.8 ± 15.3 μmol O2 h-1 larvae-1, respectively). This thermal sensitivity was not observed from 6, 8, and 14 dph larvae and juveniles at 25 dph, where morphological development was the main factor that influencing the RM. Therefore, this study shows that temperature affects RM in different development stages of totoaba, beginning in the egg stage and intensifying once the larvae hatch until 22 dph. Understanding how temperature affects energy expenditure by measuring RM is essential to establish culture conditions that allow better physiological performance and growth in the early life stages of T. macdonaldi.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44513006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clemente Balladares, Ivis Fermín, Edgar García, Juan Carlos Amilibia, Diego Rodríguez
{"title":"Preliminary analysis of microplastics from the main continental nesting beach of the hawksbill sea turtle (Eretmochelys imbricata) in Venezuela","authors":"Clemente Balladares, Ivis Fermín, Edgar García, Juan Carlos Amilibia, Diego Rodríguez","doi":"10.3856/vol51-issue1-fulltext-2789","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2789","url":null,"abstract":"Microplastics are an increasing threat to marine fauna and oceanic habitats, potentially affecting sea turtle nesting beaches. Hawksbill sea turtles (Eretmochelys imbricata) are a Critically Endangered species with decreasing population trends. There are several hawksbill rookeries in the southern Caribbean Sea, particularly on Los Garzos Beach, the main nesting site of continental Venezuela. A preliminary physical analysis of microplastics sampled from 10 sites on Los Garzos Beach reported high numbers (94 ± 2.5 items) of 14 different materials and colors. Microplastic counts at 10 sites averaged 1504 ± 405.61 items m-2, higher than other sea turtle nesting sites worldwide. There were no statistical differences in the concentration of microplastics at the sampled sites of Los Garzos Beach. However, the concentration of microplastics on this beach is higher at the midpoint of the falling tide (3520 ± 405.61 items m-2) and the top of the sea turtle nesting area (3840 ± 405.61 items m-2). We speculate that this debris could harm the nesting environment of hawksbill sea turtles in the region. While this study presents preliminary values for microplastics in an important hawksbill turtle nesting beach in Venezuela, it also serves as a baseline for long-term studies that can help determine the impact and sources of plastic pollution in sea turtle rookeries.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45482983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Factors that affect the mean size of Chilean hake (Merluccius gayi gayi) caught with gillnets in 2001-2018 in central Chile","authors":"D. Queirolo, Jorge Sateler","doi":"10.3856/vol51-issue1-fulltext-2904","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2904","url":null,"abstract":"Chilean hake (Merluccius gayi gayi) is one of central Chile's most important demersal resources, and its fishery involves an artisanal fleet that mainly uses gillnets. In the recent history of the fishery, there has been a decrease in catch sizes and a progressive reduction in mesh sizes. This study aimed to analyze the factors that influenced the mean catch sizes of Chilean hake between 2001 and 2018 to understand this situation, correlating the response of fishermen with the capture efficiency of the nets used. Applying a generalized linear model, a significant effect of the factors year, port (zone), and mesh size were observed on the mean size of individuals caught. The model explained 85% of the deviation, the greatest relative contributions being made by the factors year (53.6%) and port (30.2%). A lower effect was estimated for the mesh size factor with 1.2% deviance. These findings suggest that the progressive reduction in mesh sizes is associated with a response to the demographic change in the stock. Differentiated management measures by zones could be considered in the fishery, given the estimated differences.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42933763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ricardo Pérez-Velasco, M. Hernández‐Vergara, C. I. Pérez‐Rostro, C. Frías‐Quintana
{"title":"Variation of dietary protein/lipid levels used in postlarvae of freshwater prawn Macrobrachium rosenbergii cultured in a biofloc system","authors":"Ricardo Pérez-Velasco, M. Hernández‐Vergara, C. I. Pérez‐Rostro, C. Frías‐Quintana","doi":"10.3856/vol51-issue1-fulltext-2924","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2924","url":null,"abstract":"A 70-day experimental trial was performed to evaluate the contribution of biofloc to the nutrition of Macrobrachium rosenbergii 15 days old postlarvae (PL) (average 82.00 ± 19.71 mg) fed different crude protein (P) and lipids (L) levels in zero-water exchange culture tanks. Six biofloc treatments (BFT) as experimental diets with 15, 20, or 25% P levels and 5 or 8% L levels were managed: BFT-15/5; BFT-15/8; BFT-20/5; BFT-20/8; BFT-25/5; BFT-25/8, and a clear-water control without biofloc fed with 25% P and 8% L (CW-25/8). The experiment was done in triplicate in 21-60 L plastic tanks containing 10 prawns tank-1. At the end of the experiment, survival of prawns was above 66%, with no significant differences among experimental groups (P > 0.05). The best growth performance was observed in the experimental prawns maintained in BFT-20/5, BFT-20/8, BF-25/5, and BFT-25/8 compared to the control (P < 0.001). The feed conversion rate of the prawns in BFT-20/5 was significantly lower than that in control (P < 0.001); the best P efficiency ratio and productive P value were observed in all BFT compared to control prawns (P < 0.001). Results showed that it is feasible to use low concentrations of P (20%) and L (5%) in diets to grow giant freshwater prawn PL in a biofloc system. Results also demonstrated that biofloc contributes to the P and L requirements of cultured freshwater prawn PL, as indicated by improved feed utilization, P retention, and growth performance.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49582552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tainara Ravaglia Ferreira Gonçalves, E. P. Silva, Alan Bonner, M. Duarte
{"title":"Spatiotemporal analysis of the genetic and morphological variation of Iphigenia brasiliensis (Mollusca: Bivalvia) from the southwest tropical Atlantic","authors":"Tainara Ravaglia Ferreira Gonçalves, E. P. Silva, Alan Bonner, M. Duarte","doi":"10.3856/vol51-issue1-fulltext-2785","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2785","url":null,"abstract":"Iphigenia brasiliensis is a bivalve mollusk exploited as a shellfish and subsistence resource on the Brazilian coast, mainly in the north and northeast regions. Genetic (allozyme electrophoresis revealed eight gene loci) and morphological variations (multivariate morphometry of valves used 13 linear measurements of traditional and 19 Fourier coefficients of geometric morphometry) were studied, considering the geographic (445 km of the southwest tropical Atlantic), environmental (mangroves and channels of communication with the sea of three estuaries in the state of Rio de Janeiro), and temporal dimensions (over two years). I. brasiliensis showed high levels of genetic variation (the average number of alleles per locus ranged from 2.8 to 3.4, and the average heterozygosity ranged from 0.441 to 0.675). Bayesian analysis of population partitioning showed that the highest LnP(D) value was achieved for K = 3. These results indicated mixed ancestry, possibly oscillations in the dispersion dynamics among the different sampling groups, and temporal oscillations in the population sizes due to the anthropogenic influence on the studied estuaries. The results of morphological variation, inferred by the PERMANOVA from the Fourier analysis, indicated that a similar influence might occur in valves (also, the discriminant analysis showed that different groups could be consistently identified). In this sense, the studied populations may be organized in a dynamic of metapopulations. Finally, these are the first data on morphological and genetic variation of the species in the latitudinal, environmental, and temporal dimensions studied simultaneously, thus providing relevant information for the exploration, management, and conservation of this commercially important species.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43676050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ladilson Rodrigues Silva, Juliany Silva Mendes, Izabela Alves Paiva, V. Monteiro, Greiciene Dos Santos De Jesus, H. P. Santos, V. C. S. Coimbra, D. C. Bezerra, N. P. Bezerra
{"title":"Ectoparasitic diversity, gill alterations in Hoplerythrinus unitaeniatus (Characiformes: Erythrinidae) and Cichlasoma bimaculatum (Perciformes: Cichlidae) and quality of fishing water in the Quilombola zone in Maranhão State, Brazil","authors":"Ladilson Rodrigues Silva, Juliany Silva Mendes, Izabela Alves Paiva, V. Monteiro, Greiciene Dos Santos De Jesus, H. P. Santos, V. C. S. Coimbra, D. C. Bezerra, N. P. Bezerra","doi":"10.3856/vol51-issue1-fulltext-2936","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2936","url":null,"abstract":"The current study aimed to investigate the ectoparasitic diversity and gill alterations in Hoplerythrinus unitaeniatus (Characiformes: Erythrinidae) and Cichlasoma bimaculatum (Perciformes: Cichlidae) and evaluate the microbiological and physicochemical quality of water samples deriving from a Quilombola zone in Maranhão State, Brazil. Water samples and 42 fish specimens, 21 H. unitaeniatus and 21 C. bimaculatum, were collected from a floodable environment. Water samples were subjected to physicochemical and microbiological analyses in the laboratory environment. Fish specimens were euthanized to collect and identify ectoparasites in animals' mucus, body surface, and gills, as well as to enable the histological analysis of the second right gill arch. The herein-identified ectoparasites have shown 30.95% prevalence and comprised three phyla: Platyhelminthes, Trematoda and Arthropoda. The herein-identified main histological changes comprised incomplete and complete fusion of several lamellae, lifting of respiratory epithelium, lamellar disorganization, lamellar epithelial hyperplasia, and blood sinus dilation. The herein calculated histological alteration index has shown that 23.80% of specimens presented mild-to-moderate tissue damage, 4.77% presented moderate-to-severe tissue changes, and 9.52% presented irreparable tissue damage. It was possible concluding that histological gill lesions identified in fish specimens analyzed may be adaptive responses to the affected environment and the incidence of ectoparasites.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47426377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. F. Contente, C. Marion, João Victor Silva, M. Soeth, Mario Vinicius Lopes Condini, Lorena Lopes Almeida, Henry Louis Spach, M. Hostim-Silva
{"title":"Surf-zone fish assemblage structure and its diel variability in an ocean beach of Espírito Santo (Central Brazilian coast)","authors":"R. F. Contente, C. Marion, João Victor Silva, M. Soeth, Mario Vinicius Lopes Condini, Lorena Lopes Almeida, Henry Louis Spach, M. Hostim-Silva","doi":"10.3856/vol51-issue1-fulltext-2925","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2925","url":null,"abstract":"We characterized the surf-zone fish assemblage structure of an ocean beach on the central coast of Brazil (Guriri Beach, Espírito Santo) and assessed its diel variability. Like the entire Espírito Santo coast, Guriri Beach was also affected by ore tailings from the Fundão dam, which collapsed on November 5, 2015. Monthly samplings were carried out before the dam collapse along a year cycle (May 2013-April 2014), during the day and night in the neap tide, using a beach seine. A total of 2217 specimens were caught (1017 during the day and 1200 at night), distributed in 36 fish species and 20 families. Juveniles of the kingcroaker Menticirrhus spp., great pompano Trachinotus goodei, Florida pompano Trachinotus carolinus, littlescale threadfin Polydactylus oligodon, and Atlantic sabretooth anchovy Lycengraulis grossidens dominated the fish fauna, accounting for 84% of the total capture. The fish assemblage structure changed irregularly between day and night throughout the year. P. oligodon and Menticirrhus spp. were more abundant at night, whereas T. carolinus and L. grossidens were more abundant during the day. In most cases, no significant change was detected in fish abundance and richness between the day and night; when significant differences were detected, abundance and richness were higher at night. Our findings could support a monitoring program of the local surf-zone fish fauna and serve as before-impact data, allowing more accurate quantification of the ore tailings' impacts on the local fish biodiversity.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46375461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrés Elías Castro-Castellón, M. C. Monroy-Dosta, J. Castro-Mejía, G. Castro-Mejía, Evelyn López-García, Arnulfo Misael Martínez-Meingüer
{"title":"Evaluation of growth development and pigmentation of Heros severus cultured in a biofloc system with enriched pigment diets","authors":"Andrés Elías Castro-Castellón, M. C. Monroy-Dosta, J. Castro-Mejía, G. Castro-Mejía, Evelyn López-García, Arnulfo Misael Martínez-Meingüer","doi":"10.3856/vol51-issue1-fulltext-2935","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2935","url":null,"abstract":"This study aimed to evaluate the development and the increase in the skin pigmentation of the organism Heros severus cultured in a biofloc system with diets enriched with carotenoid pigments. The culture was made in 80 L water tanks with 20 juvenile organisms of H. severus; each treatment was made by duplicate. Four experimental diets were used: a) trout feed, El Pedregal®; b) TetraColor®; c) carrot and d) beetroot. The diet that obtained the highest values regarding the growth of the fish was the control diet. Regarding the coloration of the fish, the beetroot diet was the diet that presented the highest values with 9.55 μg of total carotenoids in tissue, presenting significant differences (P = 0.001) concerning the other diets, proving that a diet based on beetroot can be a good option for the culture of ornamental fish. Because it allows the organisms to have a survival rate above 90%, have similar growth to the control group, and a significant improvement in coloration, being a natural carotenoid low-cost source to improve the commercialization of organisms. Nevertheless, it did not present significant differences (P = 0.005) regarding beetroot and TetraColor® diets, while it presented significant differences with the carrot diet.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46715678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of macroalgae coverage in a scarcely studied deep rocky reef in the tropical eastern Mexican Pacific","authors":"Norma López, C. Candelaria, P. Ramírez-García","doi":"10.3856/vol51-issue1-fulltext-2920","DOIUrl":"https://doi.org/10.3856/vol51-issue1-fulltext-2920","url":null,"abstract":"The biodiversity of epibenthic communities in rocky reefs in the Mexican tropical Pacific has been studied minimally during the past three decades. This study describes the abundance and distribution of algae and invertebrates in a deep rocky reef from this region. Samples were taken at 20 m depth in 2012 by randomly placing 50×50 cm quadrats. Also, photographs were taken of each quadrat to quantify the coverage of organisms. Throughout the study, the algae were the most abundant group (17748.5 cm m-2), of which encrusting calcified (6350.9 cm m-2), turf (3040.3 cm m-2), and larger-sized articulated corallines (2700.9 cm m-2) had the highest coverage. Regarding invertebrates, zoanthids (1153.3 cm m-2) and corals (746.7 cm m-2) had high coverage. All of the algal groups were found on vertical and horizontal substrates. The detrended correspondence analysis showed that larger-sized articulated corallines and encrusting not calcified groups were prevalent on the horizontal substrate and corals on the vertical substrate. These abundance and distribution patterns represent the first quantitative study of rocky reefs from the region. Considering the rapid influence of human activities in this coastal zone and the fact that rocky reefs have been minimally studied, there is a clear need for long-term monitoring programs to establish reef communities' patterns and processes, which are useful in conservation programs.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44549600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessing the influence of Humboldt penguin (Spheniscus humboldti) by excrements on the levels of trace and rare earth elements in the soil","authors":"J. Celis, W. Espejo, J. Padilha, M. Sandoval","doi":"10.3856/vol50-issue5-fulltext-2933","DOIUrl":"https://doi.org/10.3856/vol50-issue5-fulltext-2933","url":null,"abstract":"Rare earth elements (REE) and some transition metals (e.g. Nb) are a group of chemicals that have recently been widely used in industrial processes due to the increasing demand for new technologies. As a result, these chemicals are increasingly being released into the environment, which could mean that these pollutants could modify marine and terrestrial ecosystems. Seabirds, such as penguins, can biotransport pollutants and nutrients from the sea to land through excreta. However, there is no information about the role of the Humboldt penguin (Spheniscus humboldti) in bio-transporting emerging contaminants such as REE. This study aimed to assess any possible contribution of Humboldt penguins to the geochemical composition of some terrestrial areas. Excreta samples were collected from Chañaral Island, one of the most important sites in Chile for the conservation of Humboldt penguins. The results showed that this species tends to contribute to soil enrichment with REE (Ce, La, Nd, and Pr) and Nb through excreta, as well as with carbon. More studies are needed to see the potential impacts on the soil.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48477039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}