Latin American Journal of Aquatic Research最新文献

{"title":"The use of different air diffusers on whiteleg shrimp, Penaeus vannamei in recirculating aquaculture system: effects on water quality and shrimp growth","authors":"Truong Giang Huynh, Thi Cam Tu Phan, Hung Hai Vu, Ngoc Ut Vu","doi":"10.3856/vol52-issue1-fulltext-2951","DOIUrl":"https://doi.org/10.3856/vol52-issue1-fulltext-2951","url":null,"abstract":"This study aimed to evaluate the effects of different air diffusers on the oxygen transfer rate, water quality, and growth performance of the whiteleg shrimp, Penaeus vannamei, for 60 days using a recirculating aquaculture system. The first experiment was conducted with six treatments applying two different air diffusers regarding two treatments (TRT): (i) rubber aeration tube and (ii) airstone at three different air pressures (0.025, 0.05, and 0.075 Mpa), respectively. Based on the first experiment's results, the second experiment was designed with two treatments: TRT1 - rubber aeration tube and TRT2 - airstone. Results indicated that the standard oxygen transfer rate increased as the pressure increased. The rubber aeration tube and air stone showed no significant difference at pressure levels. Therefore, the second experiment used a pressure level of 0.025 Mpa for TRT1 and TRT2. The results from the second trial showed that the dissolved oxygen in TRT1 did not differ significantly from that in TRT2. At the same time, the other parameters, such as temperature, pH, and alkalinity, remained steady throughout the culture period. The survival rate and biomass had the highest values in TRT2, but no significant differences were found in growth performance compared to TRT1. The results of this study suggest that either rubber aeration tubes or air stones are feasible and effective alternatives to improve the water quality and growth performance of P. vannamei in experimental conditions.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140416219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical distribution patterns of chlorophyll-a during autumn in a coastal environment inside the Gulf of California","authors":"Carlos Mauricio Torres-Martínez, Erik Coria-Monter, M. A. Monreal‐Gómez, Elizabeth Durán-Campos, D. Salas‐de‐León","doi":"10.3856/vol52-issue1-fulltext-3091","DOIUrl":"https://doi.org/10.3856/vol52-issue1-fulltext-3091","url":null,"abstract":"An evaluation of the vertical distribution of chlorophyll-a (Chl-a) levels, as an indicator of phytoplankton biomass, has strong repercussions in any marine ecosystem since it allows evaluating its productive potential and the amount of matter that is available for the higher trophic levels of the pelagic food web. This short communication aims to report the vertical distribution patterns of Chl-a levels and some selected hydrographic parameters during autumn in the Bay of La Paz, the biggest and deepest coastal environment inside the Gulf of California, a site also recognized for its high biodiversity. Two oceanographic research cruises were carried out during November 2014 and 2016. A CTD probe configured with dissolved oxygen and active fluorescence sensors, all pre-calibrated by the manufacturer, was used to acquire high-resolution data along the water column. The results showed two distribution patterns of Chl-a: 1) deep peaks (>60 m depth, with concentrations >6 mg m-3) associated with the bottom at nearshore stations, and 2) maximum concentration peaks associated with the thermocline/pycnocline with concentrations up to 7.40 mg m-3 observed at deep stations. A multivariate statistical analysis confirmed the role played by some physical variables in the distribution patterns described. The results shown in this study complement the previous research and fill in the existing gaps for the transition periods between the warm and the cold.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140410525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome characterization of Pocillopora grandis transplanted into reefs with different health conditions: potential stress indicators at the holobiont level","authors":"Ó. Juárez, Blanca Morales-Guerrero, M. Liñán-Cabello, E. Carpizo-Ituarte, Erick Delgadillo-Nuño, M. A. Delgadillo-Nuño, Ricardo Gómez-Reyes, Claudia Ventura-López, R. A. Cabral‐Tena, C. Galindo-Sánchez","doi":"10.3856/vol52-issue1-fulltext-2991","DOIUrl":"https://doi.org/10.3856/vol52-issue1-fulltext-2991","url":null,"abstract":"Understanding the potential of coral to adapt to environmental stressors that cause coral bleaching is urgent. The molecular responses of the coral holobiont to such stress conditions determine the success of symbiosis. Therefore, by characterizing molecular stress responses at the holobiont level, we can develop better tools to diagnose its health and resilience. However, only some genomic-scale resources are available for reefbuilding corals from the Eastern Tropical Pacific. This study aimed to perform a transcriptomic characterization of the Pocillopora grandis holobiont following transplantation into environments with different health conditions in Colima, Mexico. Healthy specimens of two color morphotypes (green and brown) were collected in Carrizales, a reef in good condition. Coral fragments from the two morphotypes were relocated within the source location (local transplant stress). In contrast, similar fragments were translocated into another reef with a poorer health state, La Boquita. After 24 h, the transplanted fragments were collected, and RNA-seq was performed with the Illumina system. De novo transcriptome assembly, functional annotation, identification of co-expression modules, and enrichment analysis of molecular pathways were performed. The analysis of rRNA LSU, rRNA SSU, and COI sequences confirmed the coral species, whereas analysis of Rubisco, psbA, and psaA transcripts revealed that the dominant endosymbiont was Durusdinium sp. Gene expression patterns observed across samples suggest that the transplantation to a reef with a poorer state of health affected processes such as photosynthesis, calcium homeostasis, and immune response. The transcriptomic indicators proposed here are valuable for further studies examining the adaptation of Pocillopora corals to global changes.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140411162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution to some aspects of the digestive tract anatomy and food evacuation of the Pacific fat sleeper Dormitator latrifrons","authors":"M. A. Aréchiga-Palomera, H. Nolasco‐Soria, O. Carrillo-Farnés, Karen N. Nieves-Rodríguez, F. Vega-Villasante","doi":"10.3856/vol51-issue4-fulltext-3038","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-3038","url":null,"abstract":"The development of feeding protocols and the study of gastric evacuation or passage of food through the digestive tract is essential to generate information on the digestive physiology of organisms, as they help to quantify the daily ration to avoid any poor diet. In this sense, the present work aims to demonstrate some basic aspects of the fish Dormitator latifrons digestive physiology by evaluating the transit of food through the intestinal tract as a function of time. In a completely randomized standard design, juveniles of D. latifrons were fed ad libitum with commercial pellets. Five conditioned organisms were sacrificed every hour after a single feeding. The food bolus was collected at each sampling point, using polynomial regression models to analyze the passage of food through time in the digestive tract. The passage of food showed a nonlinear behavior in time, being the simple exponential decay model of two parameters (R2 = 0.630; P < 0.0001) the best to explain the evacuation of food through the digestive tract. This knowledge will generate better feeding protocols for this species with commercial importance and aquaculture potential.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45444048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometric morphometric analysis of the variations in cephalothorax shape of the freshwater prawn Macrobrachium tenellum, Smith (Decapoda Palaemonidae) from two different coastal basins in Oaxaca, Mexico","authors":"Marcelo U. García-Guerrero, Nathali Martínez-Salazar, Andrés Ramírez-Ponce, Rodolfo De los Santos-Romero","doi":"10.3856/vol51-issue4-fulltext-3017","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-3017","url":null,"abstract":"Morphological variations of Macrobrachium tenellum prawns from two populations from locations in the Copalita and Colotepec riverine basins in the seashore of Oaxaca, Mexico, were studied with geometric morphometry techniques. The purpose was to find and explain significant differences in shape and size between the cephalothorax and the rostrum. Differences between sexes and populations were intended to prove intra and inter-populations. Results suggest low intrapopulation differences but significant interpopulation variation between specimens from different places, while a regression analysis suggests a higher correlation between shape and size only in males. The higher differences in places between and within sexes could be explained due to differences in environmental pressure. A positive allometry bias in males could indicate a sexual selection mechanism affecting mating.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45515008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproduction of Lutjanus guttatus (Perciformes: Lutjanidae) captured in the Mexican Central Pacific","authors":"G. Lucano-Ramírez, S. Ruiz-Ramírez, J. Rojo-Vázquez, R. E. Lara-Mendoza, C. Aguilar-Betancourt, G. González-Sansón","doi":"10.3856/vol51-issue4-fulltext-3008","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-3008","url":null,"abstract":"Research on the reproduction of snappers helps to manage and conserve them. The spotted rose snapper Lutjanus guttatus is a gonochoric species caught year-round in Navidad Bay, Jalisco, Mexico. This work aims to analyze the reproductive aspects of the spotted snapper L. guttatus caught in Navidad Bay on the southern coast of Jalisco, Mexico. Analyzed fish came from commercial catches. Organisms were sampled monthly from January 1998 to December 2008, and total length, total weight, sex, gonad weight, and gonadal phase were obtained for each specimen. Histological analysis was performed to define the gonadal phase and measure the oocytes' diameter. The total length range in the catches was 14.0 to 61.0 cm; 2889 females and 2792 males were collected for a sex ratio 1.0:0.97. The oocyte development is asynchronous, with a diameter range from 33.9 to 407.9 μm. The testicle is of the lobular type, with a well-defined spermatic duct. A main reproductive season from June to September was identified, although fish with mature gonads were observed in all months. The best condition was observed before the reproductive period. Average lengths at sexual maturity were 30.09 and 30.05 cm for females and males, respectively; the average length of capture in females (32.2 cm) and males (31.7 cm) corresponds to 66.6 and 64.6% of organisms that had reproduced at least once before being captured. Given the importance and continuous capture of L. guttatus in the study area, we recommend further studies to improve the understanding of reproductive and fishing activities.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42780462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic, histopathological, and non-specific immune status effect of Rhizophora mangle and Laguncularia racemose hydroalcoholic extracts against Vibrio harveyi and Vibrio campbellii in white shrimp (Penaeus vannamei)","authors":"Blanca Alicia Ramírez-Azpilcueta, N. García-Aguilar, A. Puello-Cruz, María del Carmen Bolán-Mejía, Bruno Gómez-Gil, I. Osuna-Ruíz, M. S. Morales-Covarrubias","doi":"10.3856/vol51-issue4-fulltext-3051","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-3051","url":null,"abstract":"The objective of the research was to evaluate in vitro and in vivo antibacterial efficacy of Rhizophora mangle and Laguncularia racemose when added to the feed of Penaeus vannamei in an experimental infection with Vibrio harveyi (CAIM 1792) and V. campbellii (CAIM 333). Results show that the minimum inhibitory concentration for both extracts was 1.65 g mL-1, with an inhibition zone of 25 ± 1 mm (V. campbellii); 18 ± 2 mm (V. harveyi) for L. racemose and of 21 ± 2 mm (V. campbellii); 20 ± 2 mm (V. harveyi) for R. mangle. R. mangle gave a higher total content of phenolic compounds (4.50 ± 0.26 mg GAE mL-1) and flavonoids (2.60 ± 0.15 mg QE mL-1). The challenge with V. harveyi resulted in 70% survival for organisms fed L. racemose extract and 53% for R. mangle extract. The challenge with V. campbellii resulted in 80% survival for organisms fed L. racemose and 90% R. mangle. Histopathological alterations were observed in the hepatopancreas with hemocytic infiltration within the intertubular connective tissue. Also, tubules with severe cell detachment and tubular atrophy were detected in the positive control organisms, and organisms treated with R. mangle and L. racemose only had vermiform structures in the tubular lumen, cell detachment, and infiltration hemolymph in intertubular connective tissue. According to the analysis of the studied variables, it can be concluded that the hydroalcoholic extracts of R. mangle and L. racemose reduced mortality, clinical signs, and organs and tissue alterations and improved P. vannamei immunity. It is possible to use these hydroalcoholic therapeutic extracts as treatments in shrimp-farm to enhance bacterial disease tolerance and prevent mortality.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45865105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, characterization, application, and effect of bacteria with probiotic potential from the prawn Macrobrachium tenellum in Guasave, Sinaloa, Mexico","authors":"Ruth Escamilla-Montes, Martha M. Cruz-Cervantes, Genaro Diarte-Plata, Salvador Granados-Alcantar, A. Luna-González, P. Álvarez-Ruíz, Ana S. Vega-Carranza","doi":"10.3856/vol51-issue4-fulltext-3004","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-3004","url":null,"abstract":"The prawn Macrobrachium tenellum is a species with high expectations for farming. However, like all cultivated species, it is exposed to bacterial infections caused by captivity and cultivation conditions. In this sense, probiotics in aquaculture have been used as an alternative against the excessive use of antibiotics in farming, thus preventing diseases. In the present work, 106 strains with probiotic potential were isolated, of which 77 are bacilli, and 29 are lactic acid bacteria (LAB) [(intestine (43), stomach (42) and hepatopancreas (21)] from the shrimp M. tenellum collected from a freshwater environment in Guasave, Sinaloa. Biochemical characterization of the isolated bacterial strains and molecular identification of the 16S ribosomal gene were performed. According to the hemolytic activity, the isolates MT4H2 (bacilli) and MT1E2 (LAB) showed gamma hemolysis (Ɣ), and the LAB strain presented catalase-negative. Both were Gram (+). They presented high hydrophobicity values (99.93 and 73.21%) in autoaggregation (90%) and coaggregation (99%). Regarding biofilm formation, both presented moderate capacity. On salinity tolerance, they grew in concentrations of 0 to 9% NaCl and a pH tolerance within a range of 5 to 9. Regarding enzyme activity, they exhibited activity in both proteases and lipases. Both presented resistance to some of the antibiotics tested. Only the strain of bacillus exhibited antagonistic activity against Vibrio parahaemolyticus. They were identified as Pontibacillus sp. and Pediococcus pentosaceus at a molecular level.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45595416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prochlorococcus and Synechococcus marine cyanobacteria: a scientometrics review","authors":"Caio Cesar-Ribeiro, Cristiane Santos Barbosa, Vanessa Terra, N. C. Ghisi","doi":"10.3856/vol51-issue4-fulltext-2949","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-2949","url":null,"abstract":"Prochlorococcus and Synechococcus are the dominant primary producers in the ocean and account for a significant percentage of oceanic carbon fixation. This review summarizes the available data in the literature (1992-2021; n = 480) using CiteSpace software to provide insights into the specific characteristics of Prochlorococcus and Synechococcus genes that scientists can use for establishing future research directions. From 1992 to 2021, the most frequent and with higher centrality keyword was \"multiple evolutionary origin\" (0.43), followed by flow cytometry (0.40), because this period was the main method used to identify cyanobacteria. In 2001-2010, were \"sequence\" and \"gene\", both with 0.13 of centrality; this can be explained by the period when gene sequencing was developed. In the last 10 years period 2011-2021, were diversity (0.17); marine Synechococcus (0.10); Prochlorococcus ecotype (0.08); gene expression (0.14). Additionally, the migration characteristics of flow cytometry, genes identification methods, genomes, and ecotypes of cyanobacteria, indicate how future studies should focus in 1) molecular ecology: how genetic variability among different populations affects their adaptation to different ocean environments, 2) physiology: how this phytoplankton respond to changes in nutrient availability and solar radiation, 3) interactions with other living beings: how they interact with other organisms, such as bacteria, zooplankton, and fish, and how these interactions affect the dynamics of the ocean ecosystem, and 4) modeling: computational models are being developed to predict how the distribution will change in the future as a result of climate change and human use of the oceans.                                                   ","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47084866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights on expression and purification of a recombinant luciferase protein from the bioluminescence marine dinoflagellate Pyrocystis lunula","authors":"Carlos Fajardo, F. J. Fernández‐Acero, Marcos De Donato, Fernando Gómez, Gonzalo Martínez-Rodríguez, J. Mancera","doi":"10.3856/vol51-issue4-fulltext-2898","DOIUrl":"https://doi.org/10.3856/vol51-issue4-fulltext-2898","url":null,"abstract":"Bioluminescence is interesting, among other reasons, for the various technological applications that have been derived from it. Among these applications, developing visualization techniques to record the expression of one or more genes simultaneously in real-time are particularly useful. With this in mind, this study aimed to generate a recombinant Pyrocystis lunula luciferase protein (Luci D2-3 partial CDS). As the main results, i) a fragment of 1467 bp of the luciferase (LCFb) mRNA of the dinoflagellate P. lunula, containing part of domain 2 and all of the domain 3, was cloned in the pET28a vector; ii) the constructed vector was used to transform Escherichia coli to express the recombinant protein and subsequently purify it through an affinity chromatography procedure using a His-Tag; and iii) the purified protein (~50 kDa) was further analyzed by mass spectrometry to confirm its identity. Despite being unable to perform activity tests with the luciferin substrate, the evidence from previous studies indicates that the recombinant protein obtained in this case is enzymatically active. Due to the limited number of currently available luciferases, synthesizing this recombinant protein represents a useful tool, especially in designing expression assays coupled to multiple reporter genes, thus expanding the palette of proteins available for developing this type of biotechnological advances.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48226332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}