Matrix Biology最新文献

{"title":"Inhibition of hyaluronan synthesis prevents β-cell loss in obesity-associated type 2 diabetes","authors":"Nadine Nagy ,&nbsp;Gernot Kaber ,&nbsp;Vivekananda G. Sunkari ,&nbsp;Payton L. Marshall ,&nbsp;Aviv Hargil ,&nbsp;Hedwich F. Kuipers ,&nbsp;Heather D. Ishak ,&nbsp;Marika Bogdani ,&nbsp;Rebecca L. Hull ,&nbsp;Maria Grandoch ,&nbsp;Jens W. Fischer ,&nbsp;Tracey L. McLaughlin ,&nbsp;Thomas N. Wight ,&nbsp;Paul L. Bollyky","doi":"10.1016/j.matbio.2023.09.003","DOIUrl":"10.1016/j.matbio.2023.09.003","url":null,"abstract":"<div><p>Pancreatic β-cell dysfunction and death are central to the pathogenesis of type 2 diabetes (T2D). We identified a novel role for the inflammatory extracellular matrix polymer hyaluronan (HA) in this pathophysiology. Low concentrations of HA were present in healthy pancreatic islets. However, HA substantially accumulated in cadaveric islets of T2D patients and islets of the db/db mouse model of T2D in response to hyperglycemia. Treatment with 4-methylumbelliferone (4-MU), an inhibitor of HA synthesis, or the deletion of the main HA receptor CD44, preserved glycemic control and insulin concentrations in db/db mice despite ongoing weight gain, indicating a critical role for this pathway in T2D pathogenesis. 4-MU treatment and the deletion of CD44 likewise preserved glycemic control in other settings of β-cell injury including streptozotocin treatment and islet transplantation. Mechanistically, we found that 4-MU increased the expression of the apoptosis inhibitor survivin, a downstream transcriptional target of CD44 dependent on HA/CD44 signaling, on β-cells such that caspase 3 activation did not result in β-cell apoptosis. These data indicated a role for HA accumulation in diabetes pathogenesis and suggested that it may be a viable target to ameliorate β-cell loss in T2D. These data are particularly exciting, because 4-MU is already an approved drug (also known as hymecromone), which could accelerate translation of these findings to clinical studies.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"123 ","pages":"Pages 34-47"},"PeriodicalIF":6.9,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41157730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the role of TGFβ signaling in thoracic aortic aneurysm and dissection using Fbn1 mutant mouse models","authors":"Violette Deleeuw ,&nbsp;Eric Carlson ,&nbsp;Marjolijn Renard ,&nbsp;Keith D. Zientek ,&nbsp;Phillip A. Wilmarth ,&nbsp;Ashok P. Reddy ,&nbsp;Elise C. Manalo ,&nbsp;Sara F. Tufa ,&nbsp;Douglas R. Keene ,&nbsp;Margie Olbinado ,&nbsp;Marco Stampanoni ,&nbsp;Sachiko Kanki ,&nbsp;Hiromi Yanagisawa ,&nbsp;Laura Muiño Mosquera ,&nbsp;Patrick Sips ,&nbsp;Julie De Backer ,&nbsp;Lynn Y. Sakai","doi":"10.1016/j.matbio.2023.09.001","DOIUrl":"10.1016/j.matbio.2023.09.001","url":null,"abstract":"<div><p>Although abnormal TGFβ signaling is observed in several heritable forms of thoracic aortic aneurysms and dissections including Marfan syndrome, its precise role in aortic disease progression is still disputed. Using a mouse genetic approach and quantitative isobaric labeling proteomics, we sought to elucidate the role of TGFβ signaling in three <em>Fbn1</em> mutant mouse models representing a range of aortic disease from microdissection (without aneurysm) to aneurysm (without rupture) to aneurysm and rupture. Results indicated that reduced TGFβ signaling and increased mast cell proteases were associated with microdissection. In contrast, increased abundance of extracellular matrix proteins, which could be reporters for positive TGFβ signaling, were associated with aneurysm. Marked reductions in collagens and fibrillins, and increased TGFβ signaling, were associated with aortic rupture. Our data indicate that TGFβ signaling performs context-dependent roles in the pathogenesis of thoracic aortic disease.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"123 ","pages":"Pages 17-33"},"PeriodicalIF":6.9,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts","authors":"Fiona N. Kenny ,&nbsp;Stefania Marcotti ,&nbsp;Deandra Belo De Freitas ,&nbsp;Elena M. Drudi ,&nbsp;Vivienne Leech ,&nbsp;Rachel E. Bell ,&nbsp;Jennifer Easton ,&nbsp;María-del-Carmen Díaz-de-la-Loza ,&nbsp;Roland Fleck ,&nbsp;Leanne Allison ,&nbsp;Christina Philippeos ,&nbsp;Angelika Manhart ,&nbsp;Tanya J. Shaw ,&nbsp;Brian M. Stramer","doi":"10.1016/j.matbio.2023.08.004","DOIUrl":"10.1016/j.matbio.2023.08.004","url":null,"abstract":"<div><p>Fibrosis is associated with dramatic changes in extracellular matrix (ECM) architecture of unknown etiology. Here we exploit keloid scars as a paradigm to understand fibrotic ECM organization. We reveal that keloid patient fibroblasts uniquely produce a globally aligned ECM network in 2-D culture as observed in scar tissue. ECM anisotropy develops after rapid initiation of a fibroblast supracellular actin network, suggesting that cell alignment initiates ECM patterning. Keloid fibroblasts produce elevated levels of IL-6, and autocrine IL-6 production is both necessary and sufficient to induce cell and ECM alignment, as evidenced by ligand stimulation of normal dermal fibroblasts and treatment of keloid fibroblasts with the function blocking IL-6 receptor monoclonal antibody, tocilizumab. Downstream of IL-6, supracellular organization of keloid fibroblasts is controlled by activation of cell-cell adhesion. Adhesion formation inhibits contact-induced cellular overlap leading to nematic organization of cells and an alignment of focal adhesions. Keloid fibroblasts placed on isotropic ECM align the pre-existing matrix, suggesting that focal adhesion alignment leads to active anisotropic remodeling. These results show that IL-6-induced fibroblast cooperativity can control the development of a nematic ECM, highlighting both IL-6 signaling and cell-cell adhesions as potential therapeutic targets to inhibit this common feature of fibrosis.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"123 ","pages":"Pages 1-16"},"PeriodicalIF":6.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10199306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"En route towards a personalized medicine approach: Innovative therapeutic modalities for connective tissue disorders","authors":"Charlene Redhead,&nbsp;Nandaraj Taye,&nbsp;Dirk Hubmacher","doi":"10.1016/j.matbio.2023.08.005","DOIUrl":"10.1016/j.matbio.2023.08.005","url":null,"abstract":"<div><p>Connective tissue disorders can be caused by pathogenic variants (mutations) in genes encoding extracellular matrix (ECM) proteins. Such disorders typically manifest during development or postnatal growth and result in significant morbidity and mortality. The development of curative treatments for connective tissue disorders is hampered in part by the inability of many mature connective tissues to efficiently regenerate. To be most effective, therapeutic strategies designed to preserve or restore tissue function will likely need to be initiated during phases of significant endogenous connective tissue remodeling and organ sculpting postnatally and directly target the underlying ECM protein mutations. With recent advances in whole exome sequencing, in-vitro and in-vivo disease modeling, and the development of mutation-specific molecular therapeutic modalities, it is now feasible to directly correct disease-causing mutations underlying connective tissue disorders and ameliorate their pathogenic consequences. These technological advances may lead to potentially curative personalized medicine approaches for connective tissue disorders that have previously been considered incurable. In this review, we highlight innovative therapeutic modalities including gene replacement, exon skipping, DNA/mRNA editing, and pharmacological approaches that were used to preserve or restore tissue function in the context of connective tissue disorders. Inherent to a successful application of these approaches is the need to deepen the understanding of mechanisms that regulate ECM formation and homeostasis, and to decipher how individual mutations in ECM proteins compromise ECM and connective tissue development and function.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"122 ","pages":"Pages 46-54"},"PeriodicalIF":6.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10283792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The clock transcription factor BMAL1 is a key regulator of extracellular matrix homeostasis and cell fate in the intervertebral disc","authors":"Michal Dudek ,&nbsp;Honor Morris ,&nbsp;Natalie Rogers ,&nbsp;Dharshika RJ Pathiranage ,&nbsp;Sujitha Saba Raj ,&nbsp;Danny Chan ,&nbsp;Karl E Kadler ,&nbsp;Judith Hoyland ,&nbsp;Qing-Jun Meng","doi":"10.1016/j.matbio.2023.07.002","DOIUrl":"10.1016/j.matbio.2023.07.002","url":null,"abstract":"<div><p>The circadian clock in mammals temporally coordinates physiological and behavioural processes to anticipate daily rhythmic changes in their environment. Chronic disruption to circadian rhythms (e.g., through ageing or shift work) is thought to contribute to a multitude of diseases, including degeneration of the musculoskeletal system. The intervertebral disc (IVD) in the spine contains circadian clocks which control ∼6% of the transcriptome in a rhythmic manner, including key genes involved in extracellular matrix (ECM) homeostasis. However, it remains largely unknown to what extent the local IVD molecular clock is required to drive rhythmic gene transcription and IVD physiology. In this work, we identified profound age-related changes of ECM microarchitecture and an endochondral ossification-like phenotype in the annulus fibrosus (AF) region of the IVD in the <em>Col2a1</em>-<em>Bmal1</em> knockout mice. Circadian time series RNA-Seq of the whole IVD in <em>Bmal1</em> knockout revealed loss of circadian patterns in gene expression, with an unexpected emergence of 12 h ultradian rhythms, including FOXO transcription factors. Further RNA sequencing of the AF tissue identified region-specific changes in gene expression, evidencing a loss of AF phenotype markers and a dysregulation of ECM and FOXO pathways in <em>Bmal1</em> knockout mice. Consistent with an up-regulation of FOXO1 mRNA and protein levels in <em>Bmal1</em> knockout IVDs, inhibition of FOXO1 in AF cells suppressed their osteogenic differentiation. Collectively, these data highlight the importance of the local molecular clock mechanism in the maintenance of the cell fate and ECM homeostasis of the IVD. Further studies may identify potential new molecular targets for alleviating IVD degeneration.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"122 ","pages":"Pages 1-9"},"PeriodicalIF":6.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10305044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global, but not chondrocyte-specific, MT1-MMP deficiency in adult mice causes inflammatory arthritis","authors":"Xiao-dan Xia ,&nbsp;Govind Gill ,&nbsp;Haiming Lin ,&nbsp;Daniela M. Roth ,&nbsp;Hong-mei Gu ,&nbsp;Xiang-jiang Wang ,&nbsp;Feng-yi Su ,&nbsp;Adekunle Alabi ,&nbsp;Maria Alexiou ,&nbsp;Ziyang Zhang ,&nbsp;Gui-qing Wang ,&nbsp;Daniel Graf ,&nbsp;Da-wei Zhang","doi":"10.1016/j.matbio.2023.08.003","DOIUrl":"10.1016/j.matbio.2023.08.003","url":null,"abstract":"<div><p>Membrane-type I metalloproteinase (MT1-MMP/MMP14) plays a key role in various pathophysiological processes, indicating an unaddressed need for a targeted therapeutic approach. However, mice genetically deficient in <em>Mmp14</em> show severe defects in development and growth. To investigate the possibility of MT1-MMP inhibition as a safe treatment in adults, we generated global <em>Mmp14</em> tamoxifen-induced conditional knockout (<em>Mmp14</em>^kd) mice and found that MT1-MMP deficiency in adult mice resulted in severe inflammatory arthritis. <em>Mmp14</em>^kd mice started to show noticeably swollen joints two weeks after tamoxifen administration, which progressed rapidly. <em>Mmp14</em>^kd mice reached a humane endpoint 6 to 8 weeks after tamoxifen administration due to severe arthritis. Plasma TNF-α levels were also significantly increased in <em>Mmp14</em>^kd mice. Detailed analysis revealed chondrocyte hypertrophy, synovial fibrosis, and subchondral bone remodeling in the joints of <em>Mmp14</em>^kd mice. However, global conditional knockout of MT1-MMP in adult mice did not affect body weight, blood glucose, or plasma cholesterol and triglyceride levels. Furthermore, we observed substantial expression of MT1-MMP in the articular cartilage of patients with osteoarthritis. We then developed chondrocyte-specific <em>Mmp14</em> tamoxifen-induced conditional knockout (<em>Mmp14</em>^chkd) mice. Chondrocyte MT1-MMP deficiency in adult mice also caused apparent chondrocyte hypertrophy. However, <em>Mmp14</em>^chkd mice did not exhibit synovial hyperplasia or noticeable arthritis, suggesting that chondrocyte MT1-MMP is not solely responsible for the onset of severe arthritis observed in <em>Mmp14</em>^kd mice. Our findings also suggest that highly cell-type specific inhibition of MT1-MMP is required for its potential therapeutic use.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"122 ","pages":"Pages 10-17"},"PeriodicalIF":6.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10282822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emilin2 fosters vascular stability by promoting pericyte recruitment","authors":"Albina Fejza ,&nbsp;Lucrezia Camicia ,&nbsp;Greta Carobolante ,&nbsp;Evelina Poletto ,&nbsp;Alice Paulitti ,&nbsp;Giorgia Schinello ,&nbsp;Emanuele Di Siena ,&nbsp;Renato Cannizzaro ,&nbsp;Renato V. Iozzo ,&nbsp;Gustavo Baldassarre ,&nbsp;Eva Andreuzzi ,&nbsp;Paola Spessotto ,&nbsp;Maurizio Mongiat","doi":"10.1016/j.matbio.2023.08.002","DOIUrl":"10.1016/j.matbio.2023.08.002","url":null,"abstract":"<div><p>Angiogenesis, the formation of the new blood vessels from pre-existing vasculature, is an essential process occurring under both normal and pathological conditions, such as inflammation and cancer. This complex process is regulated by several cytokines, growth factors and extracellular matrix components modulating endothelial cell and pericyte function. In this study, we discovered that the extracellular matrix glycoprotein Elastin Microfibril Interfacer 2 (Emilin2) plays a prominent role in pericyte physiology. This work was originally prompted by the observations that tumor-associated vessels from <em>Emilin2^−/−</em> mice display less pericyte coverage, impaired vascular perfusion, and reduced drug efficacy, suggesting that Emilin2 could promote vessel maturation and stabilization affecting pericyte recruitment. We found that Emilin2 affects different mechanisms engaged in pericyte recruitment and vascular stabilization. First, human primary endothelial cells challenged with recombinant Emilin2 synthesized and released ∼ 2.1 and 1.2 folds more PDGF-BB and HB-EGF, two cytokines known to promote pericyte recruitment. We also discovered that Emilin2, by directly engaging α₅β₁ and α₆β₁ integrins, highly expressed in pericytes, served as an adhesion substrate and haptotactic stimulus for pericytes. Moreover, Emilin2 evoked increased N<img>Cadherin expression via the sphingosine-1-phosphate receptor, leading to enhanced vascular stability by fostering interconnection between endothelial cells and pericytes. Finally, restoring pericyte coverage in melanoma and ovarian tumor vessels developed in <em>Emilin2^−/−</em> mice improved drug delivery to the tumors. Collectively, our results implicate Emilin2 as a prominent regulator of pericyte function and suggest that Emilin2 expression could represent a promising maker to predict the clinical outcome of patients with melanoma, ovarian, and potentially other forms of cancer.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"122 ","pages":"Pages 18-32"},"PeriodicalIF":6.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10284581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT4 protects against intestinal fibrosis by facilitating GLS1 degradation","authors":"Xinru Xue ,&nbsp;Xi Zeng ,&nbsp;Xiaoqian Wu,&nbsp;Kexin Mu,&nbsp;Yue Dai,&nbsp;Zhifeng Wei","doi":"10.1016/j.matbio.2023.08.001","DOIUrl":"10.1016/j.matbio.2023.08.001","url":null,"abstract":"<div><p>Intestinal fibrosis is a prevalent complication of Crohn's disease (CD), characterized by excessive deposition of extracellular matrix (ECM), and no approved drugs are currently available for its treatment. Sirtuin 4 (SIRT4), a potent anti-fibrosis factor in mitochondria, has an unclear role in intestinal fibrosis. In this study, fibroblasts isolated from biopsies of stenotic ileal mucosa in CD patients were analyzed to identify the most down-regulated protein among SIRT1–7, and SIRT4 was found to be the most affected. Moreover, <em>in vivo</em> and <em>in vitro</em> models of intestinal fibrosis, SIRT4 expression was significantly decreased in a TGF-β dependent manner, and its decrease was negatively associated with disease severity. SIRT4 impeded ECM deposition by inhibiting glutaminolysis, but not glycolysis, and α-ketoglutarate (α-KG) was identified as the key metabolite. Specifically, SIRT4 hinders SIRT5’s stabilizing interaction with glutaminase 1 (GLS1), thereby facilitating the degradation of GLS1. KDM6, rather than KDM4, is a potential mediator for α-KG-induced transcription of ECM components, and SIRT4 enhances the enrichment of H3K27me3 on their promotors and enhancers. These findings indicate that the activation of TGF-β signals decreases the expression of SIRT4 in intestinal fibrosis, and SIRT4 can facilitate GLS1 degradation, thereby resisting glutaminolysis and alleviating intestinal fibrosis, providing a novel therapeutic target for intestinal fibrosis.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"122 ","pages":"Pages 33-45"},"PeriodicalIF":6.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10304694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide location fingerprinting identifies structural alterations within basement membrane components in ageing kidney","authors":"Alexander Eckersley ,&nbsp;Mychel RPT Morais ,&nbsp;Matiss Ozols ,&nbsp;Rachel Lennon","doi":"10.1016/j.matbio.2023.07.001","DOIUrl":"10.1016/j.matbio.2023.07.001","url":null,"abstract":"<div><p>During ageing, the glomerular and tubular basement membranes (BM) of the kidney undergo a progressive decline in function that is underpinned by histological changes, including glomerulosclerosis and tubular interstitial fibrosis and atrophy. This BM-specific ageing is thought to result from damage accumulation to long-lived extracellular matrix (ECM) protein structures. Determining which BM proteins are susceptible to these structure-associated changes, and the possible mechanisms and downstream consequences, is critical to understand age-related kidney degeneration and to identify markers for therapeutic intervention. Peptide location fingerprinting (PLF) is an emerging proteomic mass spectrometry analysis technique capable of identifying ECM proteins with structure-associated differences that may occur by damage modifications in ageing. Here, we apply PLF as a bioinformatic screening tool to identify BM proteins with structure-associated differences between young and aged human glomerular and tubulointerstitial compartments. Several functional regions within key BM components displayed alterations in tryptic peptide yield, reflecting potential age-dependent shifts in molecular (e.g. laminin-binding regions in agrin) and cellular (e.g. integrin-binding regions in laminins 521 and 511) interactions, oxidation (e.g. collagen IV) and the fragmentation and release of matrikines (e.g. canstatin and endostatin from collagens IV and XVIII). Furthermore, we found that periostin and the collagen IV α2 chain exhibited structure-associated differences in ageing that were conserved between human kidney and previously analysed mouse lung, revealing BM components that harbour shared susceptibilities across species and organs.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"121 ","pages":"Pages 167-178"},"PeriodicalIF":6.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10233421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADAMTS3 restricts cancer invasion in models of early breast cancer progression through enhanced fibronectin degradation","authors":"Shayin V. Gibson ,&nbsp;Elizabeta Madzharova ,&nbsp;Amandine C. Tan ,&nbsp;Michael D. Allen ,&nbsp;Ulrich auf dem Keller ,&nbsp;J. Louise Jones ,&nbsp;Edward P. Carter ,&nbsp;Richard P. Grose","doi":"10.1016/j.matbio.2023.06.005","DOIUrl":"10.1016/j.matbio.2023.06.005","url":null,"abstract":"<div><p>Proteases have long been associated with cancer progression, due to their ability to facilitate invasion upon matrix remodelling. However, proteases are not simply degraders of the matrix, but also play fundamental roles in modulating cellular behaviour through the proteolytic processing of specific substrates. Indeed, proteases can elicit both pro- and anti- tumorigenic effects depending on context. Using a heterocellular spheroid model of breast cancer progression, we demonstrate the repressive function of myoepithelial ADAMTS3, with its loss directing myoepithelial-led invasion of luminal cells through a physiologically relevant matrix. Degradomic analysis, using terminal amine isotopic labelling of substrates (TAILS), combined with functional assays, implicate ADAMTS3 as a mediator of fibronectin degradation. We show further that loss of ADAMTS3 enhances levels of fibronectin in the microenvironment, promoting invasion through canonical integrin α5β1 activation. Our data highlight a tumour suppressive role for ADAMTS3 in early stage breast cancer, and contribute to the growing evidence that proteases can restrain cancer progression.</p></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"121 ","pages":"Pages 74-89"},"PeriodicalIF":6.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10206808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}