Methods in Microbiology最新文献_第7页

Solid and Suspension Microarrays for Microbial Diagnostics. 用于微生物诊断的固体和悬浮微阵列。

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 Epub Date: 2015-05-14 DOI: 10.1016/bs.mim.2015.04.002

Steve Miller, Ulas Karaoz, Eoin Brodie, Sherry Dunbar

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引用次数: 0

Virology: The Next Generation from Digital PCR to Single Virion Genomics 病毒学:从数字PCR到单病毒体基因组学的新一代

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.09.001

R. White, J. Cardenas, R. Hayden

引用次数: 1

Chapter 17 - Artificial Nucleic Acid Probes and Their Applications in Clinical Microbiology 第十七章:人工核酸探针及其在临床微生物学中的应用

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.05.003

A. Singer, Yi-Wei Tang

引用次数: 2

Clinical Applications of Quantitative Real-Time PCR in Virology. 定量实时 PCR 在病毒学中的临床应用。

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 Epub Date: 2015-07-07 DOI: 10.1016/bs.mim.2015.04.005

Julia Engstrom-Melnyk, Pedro L Rodriguez, Olivier Peraud, Raymond C Hein

{"title":"Clinical Applications of Quantitative Real-Time PCR in Virology.","authors":"Julia Engstrom-Melnyk, Pedro L Rodriguez, Olivier Peraud, Raymond C Hein","doi":"10.1016/bs.mim.2015.04.005","DOIUrl":"10.1016/bs.mim.2015.04.005","url":null,"abstract":"Since the invention of the polymerase chain reaction (PCR) and discovery of Taq polymerase, PCR has become a staple in both research and clinical molecular laboratories. As clinical and diagnostic needs have evolved over the last few decades, demanding greater levels of sensitivity and accuracy, so too has PCR performance. Through optimisation, the present-day uses of real-time PCR and quantitative real-time PCR are enumerable. The technique, combined with adoption of automated processes and reduced sample volume requirements, makes it an ideal method in a broad range of clinical applications, especially in virology. Complementing serologic testing by detecting infections within the pre-seroconversion window period and infections with immunovariant viruses, real-time PCR provides a highly valuable tool for screening, diagnosing, or monitoring diseases, as well as evaluating medical and therapeutic decision points that allows for more timely predictions of therapeutic failures than traditional methods and, lastly, assessing cure rates following targeted therapies. All of these serve vital roles in the continuum of care to enhance patient management. Beyond this, quantitative real-time PCR facilitates advancements in the quality of diagnostics by driving consensus management guidelines following standardisation to improve patient outcomes, pushing for disease eradication with assays offering progressively lower limits of detection, and rapidly meeting medical needs in cases of emerging epidemic crises involving new pathogens that may result in significant health threats.","PeriodicalId":49823,"journal":{"name":"Methods in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54117437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chapter 10 - Accurate Whole-Genome Sequencing-Based Epidemiological Surveillance of Mycobacterium Tuberculosis 第十章-基于精确全基因组测序的结核分枝杆菌流行病学监测

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.04.001

H. Pouseele, P. Supply

引用次数: 9

Molecular diagnostics in the diagnosis of parasitic infection 分子诊断在寄生虫感染诊断中的应用

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.05.001

B. Pritt

引用次数: 2

Revolutionizing Prokaryotic Systematics Through Next-Generation Sequencing 通过下一代测序革新原核生物系统学