Methods in Microbiology最新文献_第6页

Index 指数

4区生物学

Methods in Microbiology Pub Date : 2015-11-27 DOI: 10.1016/S0580-9517(15)00029-X

引用次数: 0

Next-Generation Sequencing for Pathogen Detection and Identification 新一代病原体检测和鉴定测序技术

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.06.004

K. Frey, K. Bishop-Lilly

引用次数: 14

Gene Amplification and Sequencing for Bacterial Identification 细菌鉴定的基因扩增和测序

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.04.003

S. Lau, J. L. Teng, Chi-chun Ho, P. Woo

引用次数: 13

Total Laboratory Automation in Clinical Bacteriology 临床细菌学完全实验室自动化

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.09.002

S. Novak-Weekley, E. Marlowe

引用次数: 0

Host-Based Diagnostics for Detection and Prognosis of Infectious Diseases 基于宿主的传染病检测和预后诊断

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.06.001

William E. Yang, C. Woods, E. Tsalik

引用次数: 4

Chapter 8 - Technical and Software Advances in Bacterial Pathogen Typing 第八章-细菌病原体分型的技术和软件进展

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.06.003

L. Chui, V. Li

引用次数: 2

Low-Density TaqMan® Array Cards for the Detection of Pathogens. 用于检测病原体的低密度 TaqMan® 阵列卡。

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 Epub Date: 2015-08-03 DOI: 10.1016/bs.mim.2015.06.002

Jude Heaney, Kathryn Rolfe, Nicholas S Gleadall, Jane S Greatorex, Martin D Curran

{"title":"Low-Density TaqMan® Array Cards for the Detection of Pathogens.","authors":"Jude Heaney, Kathryn Rolfe, Nicholas S Gleadall, Jane S Greatorex, Martin D Curran","doi":"10.1016/bs.mim.2015.06.002","DOIUrl":"10.1016/bs.mim.2015.06.002","url":null,"abstract":"<p><p>Real-time PCR assays have revolutionised diagnostic microbiology over the past 15 years or more. Adaptations and improvements over that time frame have led to the development of multiplex assays. However, limitations in terms of available fluorophores has meant the number of assays which can be combined has remained in single figures. This latter limitation has led to the focus tending to be on individual pathogens and their detection. This chapter describes the development of TaqMan® Array Cards (TACs), technology which allows the detection of multiple pathogens (up to 48 targets) from a single nucleic acid extract, utilising small volumes and real-time PCR. This in turn lends itself to a syndromic approach to infectious disease diagnosis. Using the examples of TACs we have developed in our own laboratory, as well as others, we explain the design, optimisation and use of TACs for respiratory, gastrointestinal and liver infections. Refinement of individual assays is discussed as well as the incorporation of appropriate internal and process controls onto the array cards. Finally, specific examples are given of instances where the assays have had a direct, positive impact on patient care.</p>","PeriodicalId":49823,"journal":{"name":"Methods in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54117592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory; Beyond Identification MALDI-TOF质谱法在临床微生物学实验室中的应用除了识别

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.04.004

S. Schubert, M. Kostrzewa

引用次数: 8

Invasive Fungal Infections and Approaches to Their Diagnosis 侵袭性真菌感染及其诊断方法

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.05.002

M. Pfaller

引用次数: 9

Molecular Strain Typing and Characterisation of Toxigenic Clostridium difficile 产毒艰难梭菌分子菌株分型及特性研究

4区生物学

Methods in Microbiology Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.07.001

T. Dingle, D. MacCannell

引用次数: 9