Pedosphere最新文献

{"title":"Exogenous application of artificial humic substance can improve black soil properties and rice yield","authors":"Yue YUAN ,&nbsp;Qingyu LIU ,&nbsp;Shun ZHANG ,&nbsp;Zhuqing LIU ,&nbsp;Xi ZHANG ,&nbsp;Kui CHENG ,&nbsp;Fan YANG","doi":"10.1016/j.pedsph.2024.05.005","DOIUrl":"10.1016/j.pedsph.2024.05.005","url":null,"abstract":"<div><div>Rice yield in the black soil region of Northeast China has been declining due to severe soil fertility degradation caused by both biotic and abiotic factors. Artificial humic substance (A-HS) has attracted much attention due to its high cost-effectiveness and great potential to improve soil fertility. However, the specific effects of A-HS on nutrient contents in rice nursery soils remain unclear. This study systematically investigated the effects of rational application of A-HS on soil nutrient turnover and yield and analyzed the changes in soil nutrients and microbial communities at Qianfeng Farm, Northeast China. The results indicated that the application of A-HS significantly increased soil dissolved organic matter and nutrient contents in the native and seedling soils. In addition, the root growth and yield of the seedlings at maturity were effectively promoted. More interestingly, the application of A-HS significantly altered plant growth-promoting rhizobacteria, such as <em>Noviherbaspirillum</em>, <em>Klebsiella</em>, and <em>Pedobacter</em>, improving natural barrier formation and soil nutrient conversion. It could be concluded that A-HS significantly enhanced crop nutrient uptake and accumulation by altering soil bacterial communities. In general, the application of A-HS could be profitable and sustainable in rice production. The current study from multiple aspects provides valuable insights into the benefits of A-HS in promoting crop growth and development, which could have important implications for agriculture and food security.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 3","pages":"Pages 504-515"},"PeriodicalIF":5.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141133812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vermicompost maintains topsoil fertility by reducing NH3 volatilization and improving 15N/NO3- retention in a saline-alkali soil","authors":"Siping LI,&nbsp;Chong WANG,&nbsp;Huiying HUANG,&nbsp;Jia CAO,&nbsp;Rui XUE,&nbsp;Binglei WANG","doi":"10.1016/j.pedsph.2024.05.008","DOIUrl":"10.1016/j.pedsph.2024.05.008","url":null,"abstract":"<div><div>Nitrogen (N) loss is a major limiting factor affecting agricultural productivity in saline-alkali soils, with ammonia (NH₃) volatilization and N leaching being the main sources of N loss. In this study, the dynamics of NH₃ volatilization were measured using the open static chamber method (sponge sampling), alongside the distribution of ¹⁵N and NO₃^--N concentrations in layers, in a 30-cm soil column experiment with vermicompost addition after incorporation of ¹⁵N-labeled urea in the upper layer (0–10 cm) of a saline-alkali soil. Destructive sampling was conducted on days 20 and 60 of the column experiment to investigate the influencing factors of NH₃ volatilization and ¹⁵N/NO₃^- retention, respectively. The results showed that the addition of vermicompost to saline-alkali soil decreased cumulative NH₃ volatilization by 45.1%, decreased the ¹⁵N concentration in the bottom layer (20–30 cm) by 17.1%, and increased the ¹⁵N concentration in the upper soil by 48.7%. Vermicompost regulated the abundances of <em>amoA</em>, <em>amoC</em>, and <em>nxrA</em> genes, which can decrease NH₃ volatilization by converting substrate NH₄⁺ to NO₃^-. Additionally, Ca²⁺ adsorption is enhanced (increased by 6.2%) by increasing soil cation exchange capacity (increased by 20.6%), thus replacing the adsorption of Na⁺ (decreased by 13.8%) and decreasing the desorption of NH₄⁺. Vermicompost enhanced the adsorption of NO₃^- by increasing Ca²⁺ and Mg²⁺ and decreasing Cl^- by 30.4% in the upper soil. This study concluded that vermicompost addition can inhibit N loss by reducing NH₃ volatilization and improving ¹⁵N/NO₃^- retention in saline-alkali soils.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 678-689"},"PeriodicalIF":5.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141133907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global change factors cause decoupling of nutrient dynamics and asynchrony between microbial communities and ecological functions in a temperate grassland soil","authors":"Yuqian LI ,&nbsp;Junwei MA ,&nbsp;Yijia LI ,&nbsp;Xinyi SHEN ,&nbsp;Xinghui XIA","doi":"10.1016/j.pedsph.2024.05.007","DOIUrl":"10.1016/j.pedsph.2024.05.007","url":null,"abstract":"<div><div>Soil microbial communities and grassland ecosystem processes are increasingly confronted with multiple global change factors (GCFs). There is still a lack of research on how these multiple GCFs interact and impact soil microbial communities and their functions. To address this gap, we conducted a simulation experiment to examine the individual and interactive effects of the four most critical and prevalent GCFs, elevated carbon dioxide concentration (eCO₂), elevated temperature (eT), decreased precipitation (dP), and elevated nitrogen (N) deposition (eN). This study focused on their effects on soil physicochemical properties, bacterial and fungal communities, and extracellular enzyme activities (EEAs) related to carbon (C), N, and phosphorus (P) cycles in a temperate grassland. Results showed that eCO₂, eN, and dP tended to increase EEAs, while having neutral effects on microbial diversity and community composition. On the other hand, eT resulted in decreases in soil pH, total C, total N, EEAs, and microbial diversity, but increases in plant biomass, total P, microbial richness, and network complexity and stability. This shift in the nutrient limitation from P to N under warming conditions resulted in decoupling of nutrients. Neutral or slightly negative relationships were found between enzyme activities and microbial richness, diversity, and dominant species, and the responses of microbial communities and ecological functions were asynchronous under GCFs. Importantly, our results revealed significant higher-order interactions among GCFs and found that they had notable effects on soil physicochemical properties as well as on microbial communities and ecological functions. These findings provide valuable insights and suggestions for ecological adaptations to future global changes.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 627-640"},"PeriodicalIF":5.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141145685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of arsenic uptake and accumulation in rice grains by applying husk-derived silicon in a synergistic way: Evidence from pot and field trials","authors":"Xianghong LI ,&nbsp;Xin WANG ,&nbsp;Xionghui JI ,&nbsp;Jingmin YANG ,&nbsp;Yunping YU ,&nbsp;Rui HUANG ,&nbsp;Bo PENG ,&nbsp;Baoshan XING","doi":"10.1016/j.pedsph.2024.05.009","DOIUrl":"10.1016/j.pedsph.2024.05.009","url":null,"abstract":"<div><div>Arsenic (As) contamination in paddy soils has posed a prominent threat to rice production in Asia. Recycling of silicon (Si) from Si-rich combusted rice husk (CRH) could serve as a sustainable strategy to mitigate rice As uptake through their shared transport pathway. Root (soil) application of CRH alone, however, was insufficient to decrease inorganic As (iAs) in polished rice below Chinese food standards (0.2 mg kg^-1). In this study, an aqueous Si solution derived from CRH was used for synergistic foliar application over the highest Si-demanding stage (reproductive stage) of rice, following root application of Si, to investigate rice As uptake in both pot and field experiments. In the pot experiment, on the basis of root application of CRH, Si supplementation before the reproductive stage of rice led to a 51% decrease in As concentration on root surface along with a prominent reduction of Fe plaque due to enhanced root suberization, relative to single root application of CRH treatment. In parallel, the expression of <em>OsLis6</em> gene in the root was downregulated by 91% than that with only root application of CRH. These changes decreased As influx into root by 56% and led correspondingly to 41% lower As transfer to the straw, as compared with root application of CRH treatment. In node I, the expression of <em>OsLis6</em> decreased concurrently by 71%, leading ultimately to 28% lower iAs accumulation in grains than that with root application of CRH alone. In the field experiment, with single foliar Si, the mitigation of grain iAs occurred only at lower soil As level of 40 mg kg^-1, while promoted iAs unloading into grains was determined under higher soil As level (80 mg kg^-1) relative to the control without Si application. It was, therefore, concluded that the mitigation of grain iAs accumulation with soil application of CRH can be strengthened critically by synergistic supply of foliar Si, serving as a more reliable pathway to secure rice production in As-contaminated paddy fields.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 763-774"},"PeriodicalIF":5.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141132255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological mechanisms of lignin- and humus-derived small molecule addition promoting straw conversion into soil organic matter in a sodic soil","authors":"Jingwang LI ,&nbsp;Lin CHEN ,&nbsp;Fengxia YUE ,&nbsp;Congzhi ZHANG ,&nbsp;Donghao MA ,&nbsp;Guixiang ZHOU ,&nbsp;Jiangli WANG ,&nbsp;Changdong HAN ,&nbsp;Biao FENG ,&nbsp;Jiabao ZHANG","doi":"10.1016/j.pedsph.2024.05.012","DOIUrl":"10.1016/j.pedsph.2024.05.012","url":null,"abstract":"<div><div>Straw return is the main practice used to increase soil organic matter (SOM) in agricultural ecosystems. To increase the efficiency of straw conversion to SOM, a large number of microbial inoculants have been developed. However, their effects are poor because of the complex water and temperature conditions, especially under sodic conditions. Small molecules can rapidly shift soil microbial communities and improve their ability to transform exogenous organic matter into SOM, providing a new direction for promoting high-efficiency straw conversion into SOM. In this study, we conducted a ¹³C-labeled straw degradation experiment using small molecules derived from lignin (LSMs) and humus (HSMs) as activators, investigating their effects on the microbial communities and formation of newly formed mineral-associated (¹³C-MAOM) and particulate (¹³C-POM) organic matter from ¹³C-labeled straw in both sodic and non-sodic soils. The ¹³C-labeled straw was mainly converted into ¹³C-MAOM, accounting for 73.97%–92.67% of the newly formed SOM. Biopolymer-derived small molecules decreased the exchangeable sodium percentage (ESP), but increased contents of ¹³C-MAOM and ¹³C-POM by shifting microbial communities, strengthening microbial cross-trophic interactions, enhancing enzyme activities, and increasing microbial residues in both soils. Addition of HSMs had greater impacts on ¹³C-MAOM formation than LSM addition. The ¹³C-MAOM and ¹³C-POM formation negatively correlated with ESP, but positively correlated with microbial cross-trophic interactions and enzyme activities in both soils. Our results suggest that biopolymer-derived small molecules promote ¹³C-MAOM and ¹³C-POM formation associated with microbial cross-trophic interactions between protistan predators and primary decomposers. Our study provides scientific support for future attempts to stimulate microbial cross-trophic interactions for boosting SOM accumulation under stressed conditions.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 603-616"},"PeriodicalIF":5.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141139178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus reward mechanisms of an arbuscular mycorrhizal fungus and a dark septate endophyte to plant carbon allocation: Synergism or competition?","authors":"Yinli BI ,&nbsp;Linlin XIE ,&nbsp;Xiao WANG ,&nbsp;Yang ZHOU","doi":"10.1016/j.pedsph.2024.05.010","DOIUrl":"10.1016/j.pedsph.2024.05.010","url":null,"abstract":"<div><div>Combined inoculation with dark septate endophytes (DSEs) and arbuscular mycorrhizal fungi (AMF) has been shown to promote plant growth, yet the underlying plant-fungus interaction mechanisms remain unclear. To elucidate the nature of this symbiosis, it is crucial to explore carbon (C) transport from plants to fungi and nutrient exchange between them. In this study, a pot experiment was conducted with two phosphorus (P) fertilization levels (low and normal) and four fungal inoculation treatments (no inoculation, single inoculation of AMF and DSE, and co-inoculation of AMF and DSE). The ¹³C isotope pulse labeling method was employed to quantify the plant photosynthetic C transfer from plants to different fungi, shedding light on the mechanisms of nutrient exchange between plants and fungi. Soil and mycelium δ¹³C, soil C/N ratio, and soil C/P ratio were higher at the low P level than at the normal P level. However, soil microbial biomass C/P ratio was lower at the low P level, suggesting that the low P level was beneficial to soil C fixation and soil fungal P mineralization and transport. At the low P level, the P reward to plants from AMF and DSE increased significantly when the plants transferred the same amount of C to the fungi, and the two fungi synergistically promoted plant nutrient uptake and growth. At the normal P level, the root P content was significantly higher in the AMF-inoculated plants than in the DSE-inoculated plants, indicating that AMF contributed more than DSE to plant P uptake with the same amount of C received. Moreover, plants preferentially allocated more C to AMF. These findings indicate the presence of a source-sink balance between plant C allocation and fungal P contribution. Overall, AMF and DSE conferred a higher reward to plants at the low P level through functional synergistic strategies.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 5","pages":"Pages 869-878"},"PeriodicalIF":7.3,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141134344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Snow removal promotes microbial-mediated organic carbon stabilization within soil aggregates in a peatland of Northeast China","authors":"Jiawen YAN ,&nbsp;Lianxi SHENG ,&nbsp;Siyuan LU ,&nbsp;Xiaofei YU ,&nbsp;Yahya KOOCH ,&nbsp;Yuanchun ZOU","doi":"10.1016/j.pedsph.2024.05.011","DOIUrl":"10.1016/j.pedsph.2024.05.011","url":null,"abstract":"<div><div>Global climate change exerts profound effects on snow cover, with consequential impacts on microbial activities and the stability of soil organic carbon (SOC) within aggregates. Northern peatlands are significant carbon reservoirs, playing a critical role in mitigating climate change. However, the effects of snow variations on microbial-mediated SOC stability within aggregates in peatlands remain inadequately understood. Here, an <em>in-situ</em> field experiment manipulating snow conditions (<em>i.e</em>., snow removal and snow cover) was conducted to investigate how snow variations affect soil microbial community and the associated SOC stability within soil aggregates (&gt; 2, 0.25–2, and &lt; 0.25 mm) in a peatland of Northeast China. The results showed that snow removal significantly increased the SOC content and stability within aggregates. Compared to the soils with snow cover, snow removal resulted in decreased soil average temperatures in the topsoil (0–30 cm depth) and subsoil (30–60 cm depth) (by 1.48 and 1.34°C, respectively) and increased freeze-thaw cycles (by 11 cycles), consequently decreasing the stability of aggregates in the topsoil and subsoil (by 23.68% and 6.85%, respectively). Furthermore, more recalcitrant carbon and enhanced SOC stability were present in microaggregates (&lt; 0.25 mm) at two soil depths. Moreover, reductions in bacterial diversity and network stability were observed in response to snow removal. Structural equation modeling analysis demonstrated that snow removal indirectly promoted (<em>P</em> &lt; 0.01) SOC stability by regulating carbon to nitrogen (C:N) ratio within aggregates. Overall, our study suggested that microaggregate protection and an appropriate C:N ratio enhanced carbon sequestration in response to climate change.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 751-762"},"PeriodicalIF":5.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141140501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen fertilization management is required for soil phosphorus mobilization by phoD-harboring bacterial community assembly and pqqC-harboring bacterial keystone taxa","authors":"Lin YANG ,&nbsp;Runze WANG ,&nbsp;Jingwei SHI ,&nbsp;Rui WANG ,&nbsp;Shengli GUO","doi":"10.1016/j.pedsph.2024.05.004","DOIUrl":"10.1016/j.pedsph.2024.05.004","url":null,"abstract":"<div><div><em>phoD</em> and <em>pqqC</em> gene occurrence in bacteria allows them to mobilize phosphorus (P) by mineralizing organic P (Po) and solubilizing inorganic P (Pi), respectively. Community characteristics of <em>phoD</em>- and <em>pqqC</em>-harboring bacteria (<em>phoD</em>- and <em>pqqC</em>-HB, respectively) mediate P cycling. However, whether the microbial community assembly and keystone taxa of <em>phoD</em>- and <em>pqqC</em>-HB regulate P availability and distinct regulatory pathways between these two genes remain unclear. In this study, soil microbial community characteristics and P availability were investigated in four long-term (38-year) fertilization regimes: control with no fertilizer (CK), P fertilizer (PF), nitrogen (N) and P fertilizers (NP), and N fertilizer, P fertilizer, and manure (NPM). The N addition treatments (NP and NPM) significantly changed the community composition and increased the abundances of <em>phoD</em>- and <em>pqqC</em>-HB compared to the no-N addition treatments (CK and PF). Stochastic processes dominated the community assembly of both <em>phoD</em>- and <em>pqqC</em>-HB, and the relative contributions of stochasticity increased with N addition. Furthermore, the N addition treatments resulted in greater network complexity and higher abundances of keystone taxa of <em>phoD</em>- and <em>pqqC</em>-HB compared to those of the no-N addition treatments. The keystone taxa implicated in P cycling were also associated with carbon (C) and N cycling processes. Microbial community composition and assembly processes were the main factors driving labile Pi for <em>phoD</em>-HB, whereas keystone taxa contributed the most to labile Pi for <em>pqqC</em>-HB. These results emphasize that distinct mechanisms of <em>phoD</em>- and <em>pqqC</em>-HB regulate P availability under fertilization management and underline the significance of microbial community assembly and keystone taxa in soil ecological functions, offering fresh perspectives on comprehending the biological processes facilitated by microorganisms in enhancing soil quality.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 655-666"},"PeriodicalIF":5.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141041769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal patterns in diversity, complexity and community assembly of soil microorganisms in a subtropical coastal wetland","authors":"Guiping YE ,&nbsp;Nan YANG ,&nbsp;Ziyang HE ,&nbsp;Ping YANG ,&nbsp;Ruichao YE ,&nbsp;Miaohua JIANG ,&nbsp;Dan WANG ,&nbsp;Dingding CAO ,&nbsp;Wenbin ZHANG ,&nbsp;Xiangying WEI ,&nbsp;Yongxin LIN","doi":"10.1016/j.pedsph.2024.05.001","DOIUrl":"10.1016/j.pedsph.2024.05.001","url":null,"abstract":"<div><div>Soil microbiomes are significant for biodiversity, crucial for ecosystem functions, and vital for the health of various organisms. Nevertheless, the impacts of season and plant species shifts on soil microbial diversity and community assembly are still poorly understood. This study explored soil bacterial, fungal, and protistan communities during summer and winter in a coastal wetland affected by <em>Spartina alterniflora</em> invasion and subsequent <em>Cyperus malaccensis</em> or <em>Kandelia obovata</em> restoration. The results showed that bacterial, fungal, and protistan diversity were 2.63%, 40.3%, and 9.90% higher in winter than in summer, respectively. Plant species had a distinct impact on microbial diversity. Notably, <em>K. obovata</em> restoration significantly increased bacterial diversity, but decreased protistan diversity, with no effect on fungal diversity when compared to <em>S. alterniflora</em> invasion. Season and plant species both significantly influenced the community structure of bacteria, fungi, and protists. However, protistan community structure was more sensitive to season compared to the structure of bacterial and fungal communities. The complexity of co-occurrence networks within or among bacteria, fungi, and protists was higher in winter than in summer. Bacterial and protistan community assembly was primarily driven by stochastic processes, while fungal assembly was dominated by deterministic processes. Bacterial and protistan community assembly exhibited lower stochasticity in winter compared to summer, suggesting a more deterministic assembly of communities during winter. Our findings highlight the critical role of season and plant species in regulating microbial communities, revealing higher microbial diversity, network complexity, and determinism in community assembly during winter compared to summer in a subtropical coastal wetland.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 728-740"},"PeriodicalIF":5.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141057625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial distribution patterns and driving factors related to nitrogen cycling in karst Tiankeng soil","authors":"Xiaohui WANG,&nbsp;Junbo YANG,&nbsp;Keyi WANG,&nbsp;Danjuan ZENG,&nbsp;Ling MO,&nbsp;Gaozhong PU","doi":"10.1016/j.pedsph.2024.05.003","DOIUrl":"10.1016/j.pedsph.2024.05.003","url":null,"abstract":"<div><div>Soil nitrogen (N) cycling is one of the most critical biogeochemical cycles, and N cycling-related microorganisms are the primary driving force behind N cycling in natural environments. The large karst sinkholes in China, known as Tiankengs, harbor abundant unique biological resources due to their particular environmental conditions. However, N cycling-related microorganisms in Tiankeng soils and their connection to ecosystem processes remain poorly studied. In this study, we investigated the distribution patterns and genomic diversity of N cycling-related microorganisms both inside and outside the Luohun cave Tiankeng in Guizhou, China, utilizing high-throughput sequencing and other techniques. The results indicated that the diversities and abundances of denitrifying bacteria, ammonia-oxidizing bacteria, and ammonia-oxidizing archaea communities inside the Tiankeng were higher than those outside the Tiankeng; however, the microbial network relationships were more fragile inside the Tiankeng. The most abundant species of denitrifying bacteria, ammonia-oxidizing bacteria, and ammonia-oxidizing archaea inside the Tiankeng were unclassified_p_Proteobacteria (47.8%), unclassified_k_norank (AOB, OTU121, 37.3%), and unclassified_g_norank_f_norank_o_norank_c_environmental_samples (55.7%), respectively; outside the Tiankeng, they were unclassified_k_norank_d_bacteria (54.5%), unclassified_k_norank (AOB, OTU121, 48.1%), and unclassified_k_norank (AOA, OTU70, 49.6%), respectively. Additionally, the N content inside the Tiankeng was significantly lower (<em>P</em> &lt; 0.05) under the influence of these N cycling-related microorganisms, whereas the nutrient contents were higher than that outside the Tiankeng. To the best of our knowledge, this is the first report on the crucial microbial distribution patterns driving N cycling in karst Tiankengs and provides new insights into the structure and potential functions of N cycling-related microorganisms in the unique ecological environment of fragile Tiankeng ecosystems.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 4","pages":"Pages 706-714"},"PeriodicalIF":5.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}